Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 71,990 A→G 9.1% N328N (AAT→AAC)  arcD1 ← arginine/ornithine APC family amino acid‑polyamine‑organocation transporter, antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073071,9900AG 9.1% 117.4 / 3.9 44N328N (AAT→AAC) arcD1arginine/ornithine APC family amino acid‑polyamine‑organocation transporter, antiporter
Reads supporting (aligned to +/- strand):  ref base A (18/22);  new base G (2/2);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACCAAGCTCCTAAAACAGAGATAATTAAGCCTATATTAACTAACACTGAACCCCAA  >  CP000730/71851‑72128
                                                                                                                                           |                                                                                                                                          
gtgtATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAGTAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTAt                                                                                                                                           <  1:263360/141‑1 (MQ=255)
  gtATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTTACCTGAGCTTTATTCTTAttt                                                                                                                                         >  2:148026/1‑141 (MQ=255)
    atatGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTc                                                                                                                                       <  1:91906/141‑1 (MQ=255)
       tGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCttt                                                                                                                                    >  1:105862/1‑141 (MQ=255)
          aaTTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGc                                                                                                                                 >  1:83502/1‑141 (MQ=255)
              aaGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAAc                                                                                                                             >  1:97711/1‑141 (MQ=255)
                         tGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTGTTTTCTTTAGCAAACCATTTCGGGaa                                                                                                                  >  1:58590/1‑141 (MQ=255)
                         tGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTGTTTTCTTTAGCAAACCATTTCGGGaa                                                                                                                  >  1:102785/1‑141 (MQ=255)
                         tGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGCaa                                                                                                                  >  2:265062/1‑141 (MQ=255)
                              cAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTc                                                                                                             <  2:213014/141‑1 (MQ=255)
                                aGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAGAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCa                                                                                                           <  2:236080/141‑1 (MQ=255)
                                           aaaCTGGTAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTAc                                                                                                <  1:329544/141‑1 (MQ=255)
                                              cTGCTAGGCACTATCTGTAAACCCGATTCGAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACaat                                                                                             <  1:77530/141‑1 (MQ=39)
                                                     gCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGt                                                                                      <  1:314529/141‑1 (MQ=255)
                                                     gCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGCAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGt                                                                                      <  1:248142/141‑1 (MQ=255)
                                                              gTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCa                                                                             >  1:53977/1‑141 (MQ=255)
                                                                aaaCAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATACTTGCCGTGTCGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAAGGGTAATTCACCAGc                                                                           <  1:179537/141‑1 (MQ=38)
                                                                  acaacaTACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTa                                                                         <  2:97711/141‑1 (MQ=255)
                                                                    aacaTACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGt                                                                       <  2:342393/141‑1 (MQ=255)
                                                                    aacaTACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGt                                                                       <  1:108004/141‑1 (MQ=255)
                                                                    aacaTACCAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGt                                                                       <  2:177068/141‑1 (MQ=255)
                                                                       aTACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATATTTAGCTACCACGAATGGTAATTCACCAGCGAGTAAt                                                                    <  2:201222/141‑1 (MQ=255)
                                                                                   aaTAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAAc                                                        >  1:162525/1‑141 (MQ=255)
                                                                                         tGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCCTTTCAGGAAACGACCAAATAATGTTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACCAAGCt                                                  >  1:64856/1‑141 (MQ=37)
                                                                                          gAACTAATATATTAGTAATAATTAAAACGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACGAAGCTc                                                 >  2:213955/1‑141 (MQ=255)
                                                                                            aCTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTGTTGTCCATCCTAACCAAGCACCt                                               >  1:28079/1‑141 (MQ=255)
                                                                                            aCTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACCACGCTCCt                                               >  2:174400/1‑141 (MQ=255)
                                                                                                atatatTAGTAATAATTAAAGCGTAGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTGACCAGCTAGTAATGTCCATCCTAACAAAGCTCCTaaac                                           >  2:230163/1‑140 (MQ=255)
                                                                                                       aGTAATTATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATTGTAATTCACCAGCTAGTAATGTCCATCCTAACCAAGCTCCTAAAACAGAGAt                                    <  2:314456/141‑1 (MQ=255)
                                                                                                       aGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACCAAGCTCCTAAAACAGAGAt                                    <  2:83502/141‑1 (MQ=255)
                                                                                                        ttaataaTTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACCAAGCTCCTAAAACAGAGATa                                   <  2:197030/140‑1 (MQ=255)
                                                                                                        gTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACCAAGCTCCTAAAACAGAGATa                                   <  2:345002/141‑1 (MQ=255)
                                                                                                         taataaTTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACCAAGCTCCTAAAACAGAGATaa                                  >  2:340585/1‑141 (MQ=255)
                                                                                                               ttAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATACTCCCCACTCGCCTAAAACAGAGATAATGAAGc                            >  1:161327/1‑141 (MQ=38)
                                                                                                                tAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACCAAGCTCCTAAAACAGAGATAATTAAGcc                           <  2:53977/141‑1 (MQ=255)
                                                                                                                       gtgcttggAGCCTTATTCTTATTTTCTTGAGCAAACCATATCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACCAAGCTCCTAAAACAGAGATAATTAAGCCTATATta                    <  2:204705/135‑1 (MQ=255)
                                                                                                                         gACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACCAAGCTCCTAAAACAGAGATAATTAAGCCTATATtaac                  <  1:123594/141‑1 (MQ=255)
                                                                                                                          aCCGGAGCTTTATTCTTATTTTCTTTAGCAAACAATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACCAAGCTCCTAAAACAGAGATAATTAAGCCTATATtaact                 >  2:56962/1‑141 (MQ=255)
                                                                                                                               aGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACCAAGCTCCTAAAACAGAGATAATTAAGCCTATATTAACTAacac            <  2:215078/141‑1 (MQ=255)
                                                                                                                                     aTTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACCAAGCTCCTAAAACAGAGATAATTAAGCCTATATTAACTAACACTGAAcc      >  1:258130/1‑141 (MQ=255)
                                                                                                                                       tCTTGTTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACCAAGCTCCTAAAACAGAGATAATTAAGCCTATATTAACTAACACTGAAcccc    <  2:58590/141‑1 (MQ=255)
                                                                                                                                       tCTTGTTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACCAAGCTCCTAAAACAGAGATAATTAAGCCTATATTAACTAACACTGAAcccc    <  2:102785/141‑1 (MQ=255)
                                                                                                                                         ttATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACATTGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACCAAGCTCCTAAAACAGAGATAATTAAGCCTATATTAACTAACACTGAACCCCaa  >  1:119441/1‑141 (MQ=255)
                                                                                                                                         ttATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACCAAGCTCCTAAAACAGAGATAATTAAGCCTATATTAACTAACACTGAACCCCaa  >  1:119459/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAACCATTTCGGGAAAAGTCCATCTTTAGCTACAATGAATGGTAATTCACCAGCTAGTAATGTCCATCCTAACCAAGCTCCTAAAACAGAGATAATTAAGCCTATATTAACTAACACTGAACCCCAA  >  CP000730/71851‑72128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑