Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,679,462 (A)8→7 100% intergenic (+334/‑55) USA300HOU_2535 → / → USA300HOU_2536 hypothetical protein/possible secretory antigen

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,679,4550A.92.4% 310.3 / 14.8 78intergenic (+327/‑62)USA300HOU_2535/USA300HOU_2536hypothetical protein/possible secretory antigen
Reads supporting (aligned to +/- strand):  ref base A (0/5);  new base . (47/25);  total (47/31)
Fisher's exact test for biased strand distribution p-value = 7.21e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

TTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTCACTTATCA  >  CP000730/2679316‑2679590
                                                                                                                                           |                                                                                                                                       
ttGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTa                                                                                                                                         >  1:699755/1‑140 (MQ=255)
        cTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTCTTGTTGATTGCTTCCATTGTTTTCTaacaaaatt                                                                                                                                 >  2:35542/1‑138 (MQ=255)
          attaCATTTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGAGTGCTTAACTTGTTTTCTAAAAAAAtttg                                                                                                                               <  2:442865/137‑2 (MQ=255)
          aTGACAATTAAGTGGTAAATACCTGAATTTCTACATTCTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAAtttg                                                                                                                               <  2:176337/140‑2 (MQ=255)
           tGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGt                                                                                                                             >  1:656712/1‑140 (MQ=255)
            gACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGtt                                                                                                                            >  1:34125/1‑140 (MQ=255)
              cAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACTATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTAt                                                                                                                          >  1:438544/1‑140 (MQ=255)
              cAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTAt                                                                                                                          >  1:305905/1‑140 (MQ=255)
                 ttaattGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCAt                                                                                                                       <  2:517560/140‑1 (MQ=255)
                  taattGTTAAATACATCAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAAGTCAACACTGTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATa                                                                                                                      <  2:173691/140‑1 (MQ=255)
                    attGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAAt                                                                                                                    >  2:307982/1‑140 (MQ=255)
                     ttGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAAtt                                                                                                                   >  1:565655/1‑140 (MQ=255)
                      tGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTa                                                                                                                  >  2:409386/1‑140 (MQ=255)
                        ttAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTATTATTTCATCAAAGTGTTTTCTAAAAAAATTTGTTATAATAATTaaa                                                                                                                >  1:312792/1‑139 (MQ=255)
                           aaTACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACGGTTTCTAATATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATGATTAACGtt                                                                                                             >  1:167568/1‑140 (MQ=255)
                            aTACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGCTTGGTTACAATGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTg                                                                                                            >  1:198637/1‑140 (MQ=255)
                            aTACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTg                                                                                                            >  1:308430/1‑140 (MQ=255)
                                aTGAATTTTTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATa                                                                                                        <  1:29322/140‑1 (MQ=255)
                                aTGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGaaaa                                                                                                        >  1:152459/1‑138 (MQ=255)
                                  gAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATaaa                                                                                                      <  1:7185/140‑1 (MQ=255)
                                  gAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATaaa                                                                                                      <  1:354945/140‑1 (MQ=255)
                                     tttCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGaa                                                                                                   <  1:575371/140‑1 (MQ=255)
                                           cATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAAt                                                                                             >  1:434625/1‑140 (MQ=255)
                                           cATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAAt                                                                                             >  1:653268/1‑140 (MQ=255)
                                           cATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAAt                                                                                             >  1:540422/1‑140 (MQ=255)
                                            aTTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAAtt                                                                                            <  2:5962/140‑1 (MQ=255)
                                               ttATGAAAAAATACAGTTTTAATACAATTCAACACTTTATATGAGAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAAc                                                                                         <  1:479261/140‑1 (MQ=255)
                                                  tGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTg                                                                                      <  2:577143/140‑1 (MQ=255)
                                                            cATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTAAAAAAAAAAAAAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATaaaa                                                                            <  2:656149/140‑1 (MQ=255)
                                                              tttttATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAAt                                                                          >  2:588193/1‑140 (MQ=255)
                                                               ttttATTACAATTCAACACTTTCTCTGGGAACATCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCTTATTTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATg                                                                         >  1:352560/1‑140 (MQ=255)
                                                                   attaCAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAat                                                                     <  2:305905/140‑1 (MQ=255)
                                                                    ttaCAATTCAACACTTTATATGACAAATTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAata                                                                    >  2:699263/1‑140 (MQ=255)
                                                                     taCAATTCAACACCTTATATGACAACCTCAATGAAGTTAGTTTTAATCCTTATTTCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGTAGATAAAAATGGAatat                                                                   <  2:150229/140‑1 (MQ=255)
                                                                      aCAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAAtata                                                                  >  1:584697/1‑140 (MQ=255)
                                                                      aCAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAAtata                                                                  <  1:330918/140‑1 (MQ=255)
                                                                             aaCACTTTATATGACTACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGAt                                                           >  1:539512/1‑140 (MQ=255)
                                                                             aaCACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGAt                                                           >  1:468050/1‑140 (MQ=255)
                                                                               cacTTTATATGACATCTTCATTACCGTTACTTTGATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGATAAAAAAAAAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATAc                                                         <  2:376978/140‑1 (MQ=255)
                                                                               cacTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATAc                                                         >  1:606853/1‑140 (MQ=255)
                                                                               cacTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATAc                                                         >  1:587299/1‑140 (MQ=255)
                                                                                acTTTATATGACTAGTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACt                                                        >  2:337469/1‑140 (MQ=255)
                                                                                acTTTATATGACAAGTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACt                                                        >  1:388801/1‑140 (MQ=255)
                                                                                acTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACt                                                        >  1:664016/1‑140 (MQ=255)
                                                                                acTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTACTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAACAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACt                                                        >  2:224595/1‑140 (MQ=255)
                                                                                 cTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTa                                                       >  2:115364/1‑140 (MQ=255)
                                                                                   ttATATGACAACTTCATTACAGTTATTTTTATTGTTGATTGCTTACATTGTTTTTTAAAAAAATTTGTTATCATAATTAACTTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAAt                                                     <  1:444342/140‑1 (MQ=255)
                                                                                   ttATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTCGGACAGCAAAATGGAATATAAAAAGATACTAAt                                                     <  2:699755/140‑1 (MQ=255)
                                                                                   ttATATGACAACTTCATTACAGTTACTTTTATCTTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATATTAAt                                                     <  1:494431/140‑1 (MQ=255)
                                                                                      tatGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCg                                                  >  2:437870/1‑140 (MQ=255)
                                                                                       atGACTACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGt                                                 <  2:421552/140‑1 (MQ=255)
                                                                                       atGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGt                                                 >  1:427166/1‑140 (MQ=255)
                                                                                              cTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGACAAAAATGGGATATAAAAAGATACTAATTCGTTTATTaa                                          <  1:661894/140‑1 (MQ=255)
                                                                                                  aTTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAACATTTCTTATCCTAACTAACGTTGAATAAAGACCCAAATGAAATTGGGAGAAGAAAAGGGAAGACAAAAGAATACTAAGTCGATTATTAATTGc                                      >  2:422155/1‑140 (MQ=255)
                                                                                                  aTTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTATTTCGTTTATTAATTGc                                      >  2:222329/1‑140 (MQ=255)
                                                                                                  aTTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGAAATATAAAAAGAAACTAATTCGTTTATTAATTga                                      >  1:82062/1‑139 (MQ=255)
                                                                                                  aTTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGATGAATAAAGAAAAAAATTAAATTGGGAGATAAAAAGGAGATATGAAAAGATACTAATTCGTTTATTAATTGc                                      >  2:685254/1‑140 (MQ=255)
                                                                                                   ttACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCt                                     >  2:332895/1‑140 (MQ=255)
                                                                                                   ttACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCt                                     >  1:383156/1‑140 (MQ=255)
                                                                                                   ttACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATGAATTGCt                                     >  1:384817/1‑140 (MQ=255)
                                                                                                   ttACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAAATACTAATTCGTTTCTTAATTGCt                                     >  2:332896/1‑140 (MQ=255)
                                                                                                   ttACAGTTACTTTTATTGGTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTAAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTgat                                     >  2:83868/1‑138 (MQ=255)
                                                                                                    tACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTACtt                                    >  1:490119/1‑140 (MQ=255)
                                                                                                            cTTTTATTGTTGATTACTTAAAAAATATTAAAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTa                            <  2:673114/140‑1 (MQ=255)
                                                                                                              tttATTGTTGATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACt                          >  1:1995/1‑140 (MQ=255)
                                                                                                                     ttgATTGCTTACATTGATTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTca                   <  2:52180/140‑1 (MQ=255)
                                                                                                                      ttaatactaaCATTATTTTCAAAAAAAATTTGTTATCAAAATTAACAAGGAAAAAAGAAAAAAATTAACTGGGGAGACAAAAATGGAATATAAAAAGATACTAATTCTTTTATTAATTGCTTTTGCAGTACTTTTCCcag                  <  2:352560/131‑1 (MQ=255)
                                                                                                                      ttaTTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATGATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCATTACTTTTCTcag                  <  1:513874/138‑1 (MQ=255)
                                                                                                                      tcaTTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTcag                  <  1:606192/138‑1 (MQ=255)
                                                                                                                       gATTGCTTACATTGTTTTCTAAAAAATTTTGTTATCATAATTAACGTTCAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTcagc                 <  1:165024/140‑1 (MQ=255)
                                                                                                                       gATTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGGAGTACTTTTCTcaag                 >  2:303544/1‑138 (MQ=255)
                                                                                                                        aTTGCTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTcagca                <  1:409386/140‑1 (MQ=255)
                                                                                                                           tcTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGAt             <  2:540422/139‑1 (MQ=255)
                                                                                                                            cTTACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGAtt            <  1:337469/140‑1 (MQ=255)
                                                                                                                              tACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTc          >  2:716030/1‑140 (MQ=255)
                                                                                                                              tACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTc          >  2:104567/1‑140 (MQ=255)
                                                                                                                              tACATTGTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAAGAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTc          >  2:387767/1‑140 (MQ=255)
                                                                                                                                    gTTTTCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAAAGGAATATAAAAAAATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTCACTTAt    >  1:51477/1‑140 (MQ=255)
                                                                                                                                     ttttCTAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTCACTTATc   <  2:346993/140‑1 (MQ=255)
                                                                                                                                      tttCTAAAAAAATTTTTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTCACTTATCa  >  1:538391/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                       
TTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTCACTTATCA  >  CP000730/2679316‑2679590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: