| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 454,656 | G→A | 100% | V119I (GTT→ATT) | set23 → | staphylococcal exotoxin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 454,656 | 0 | G | A | 98.4% | 167.2 / ‑4.7 | 61 | V119I (GTT→ATT) | set23 | staphylococcal exotoxin |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (23/37); minor base T (1/0); total (24/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTTTTAACCAACAAAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTGTTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCAAAAGGAAGAAGT > CP000730/454522‑454789 | tttttAACCAACAACATCACAAGTTCCAAGTTTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATttagtattagt < 1:130372/140‑1 (MQ=255) aCCAACAAAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTa < 2:290351/140‑1 (MQ=255) caacaaAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATCTTTAGTATAACTGGAGTAAc > 1:357190/1‑140 (MQ=255) caaAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACaaa < 1:657274/140‑1 (MQ=255) aaaTCAATAGTTCCAAGTGTGTTTATTGGGAAAAGATGAAAATAAATACAAAGACAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGAAGATCTAGATGGAAGAATATTAAGTATTAGTGGTGTAACAAAGa > 1:458850/1‑140 (MQ=255) aaTCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGaa > 2:549935/1‑140 (MQ=255) tCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGaaaa > 2:198397/1‑140 (MQ=255) cAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGaaaaa > 2:416169/1‑140 (MQ=255) cAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGaaaaa > 2:584519/1‑140 (MQ=255) aaGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGt < 2:513156/140‑1 (MQ=255) tCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTaaaa < 1:456413/140‑1 (MQ=255) tCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTaaaa < 1:244279/140‑1 (MQ=255) cAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGGGGTGTGACAAAGAAAACGGTAAAGTc > 2:232986/1‑140 (MQ=255) cAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTCAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATc < 1:251720/140‑1 (MQ=255) atGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGATTAGATGTCTTTGCGGTACCATAATTAGTAGATTTAGAAGGAAGAATATTTAGTATTAGGGGTGTAACAGAGAAAAACGTAAAATCAATATTTGAgac > 2:286224/2‑138 (MQ=255) aaaattaaaaTAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATGTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGa < 1:120729/134‑1 (MQ=255) aaGATGAAAATAAATACAAAGAAAAAACAAATGGTTTAGATCTCTTTACTGATCCGGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGa < 1:164311/140‑1 (MQ=255) cgATGAAATTAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACAAGAATTAGTAGATTTAGATGGAAGAATATATAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTagaaa > 2:28378/2‑136 (MQ=255) aaataaaaaaaaaTACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGaa < 2:623047/135‑1 (MQ=255) aGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGaa < 1:326347/140‑1 (MQ=255) aGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGaa < 2:723100/140‑1 (MQ=255) gATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATAAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAGGaaa > 1:627897/1‑139 (MQ=255) attaaaaTAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACg < 1:698398/137‑1 (MQ=255) taCAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACt < 1:275866/140‑1 (MQ=255) aaaaaaGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTATAACAAAGAAAACCTTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACt < 2:662624/137‑1 (MQ=255) cAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAg < 1:92188/140‑1 (MQ=255) gaGAAAATGCACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGtt < 1:673327/139‑1 (MQ=255) aaaaCAGATGGTTTAGATGTCATTGCGGTACCAGAATTAGTAGATATAGATGGAAGAATATTTAGTAATAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTaaaaa < 2:81212/140‑1 (MQ=255) aaaCACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACAAGTTaaaaaa > 1:458685/1‑140 (MQ=255) acacATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAAt > 1:690689/1‑140 (MQ=255) acaTGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTTAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAg > 1:260716/1‑140 (MQ=255) aGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTAGTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAATAACGCCGAACTTACTAGTGAATAAAATAGACGATAAAg < 1:406472/140‑1 (MQ=255) aTGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGAc < 1:549935/140‑1 (MQ=255) ggcattGCGGCACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATCAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGCACTTACTAGTTAACAAAATAGACGATAAAGACgg < 1:322463/136‑1 (MQ=255) gTCTTTGCGGTACCATAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACgg < 1:63280/140‑1 (MQ=255) gTCTTTGCGGTACCAGAATTAGTAGATTTAGATGTAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACgg < 1:288890/140‑1 (MQ=255) gTCTTTGCCGTACCAGAATTAGAAGATTTAGATCGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAACATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACgg < 1:422005/140‑1 (MQ=255) tCTTTGCGGTACCAGAATTAGTAGATTTGGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGt > 2:248940/1‑140 (MQ=255) tCTTTACGGTACCAGAATTAGTAGATTTAGATGGAAGAATAGTTAGTATTGGTGGTGTAACAAAGCAAAACGTAAAATAACTATTGGAATGTCTAAGAACGACGAAATTACTACTTAACAAAATAAACGATAAAGAccgc > 2:134576/1‑137 (MQ=255) tttGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTATCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGttt > 2:473346/1‑140 (MQ=255) cGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACAGTAAAAACGGATTTTca > 1:344147/1‑139 (MQ=255) ccAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATCAAGACGGTTTTTCTATtga < 1:435695/140‑1 (MQ=255) ccAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATtga < 1:114243/140‑1 (MQ=255) aGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATtgatg < 1:577025/140‑1 (MQ=255) aGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTAAAAAAAATAGACGATAAAGACGGTTTTTCTATtgatg < 2:232911/140‑1 (MQ=255) aaTTAGTAGATTTAGATGGAAGAATATTTAGTATCAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTATATTGATGaa > 1:103304/1‑140 (MQ=255) tagtagATTTAGATGGAAGAATATTGCGATGCAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGATTTTCTATTGATGAAttt > 1:352850/1‑140 (MQ=255) agtagATTTATATGGAAGAATATTTAGTAATAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAAATAAATAATAAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAAtttt < 2:375225/140‑1 (MQ=255) agtagATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAAtttt < 2:356104/140‑1 (MQ=255) tagATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTc > 2:448619/1‑140 (MQ=255) gATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCtt < 1:416654/140‑1 (MQ=255) ttttAGATGGAAATATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAACGAATATTTGAGACTAAAAGAAAGCCGTACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCttt < 1:28378/139‑1 (MQ=255) tAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTAtt < 1:198397/140‑1 (MQ=255) aGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTc > 1:68770/1‑140 (MQ=255) ggAAGAATATTTAGTATTAGTAGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCaaaa < 1:498941/140‑1 (MQ=255) gaagaaTATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTGAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCAAAAg > 2:256937/1‑140 (MQ=255) agaaTATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCAAAAGga > 1:313893/1‑140 (MQ=255) agaaTATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTAAAAAGga > 1:654052/1‑140 (MQ=255) atatTTAGTATGAGTGGTTTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCAAAAGgaaga < 2:140119/140‑1 (MQ=255) tatTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGTCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCAAAAGgaagaa < 1:193960/140‑1 (MQ=255) ttttGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCAAAAGGAAGAAgt < 2:357190/140‑1 (MQ=255) tttAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCAAAAGGAAGAAgt < 1:448619/140‑1 (MQ=255) | TTTTTAACCAACAAAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTGTTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCAAAAGGAAGAAGT > CP000730/454522‑454789 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |