Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,232,465 G→C 100% A124P (GCG→CCG)  USA300HOU_1157 → non‑specific serine/threonine protein kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,232,4650GC100.0% 240.4 / ‑6.5 77A124P (GCG→CCG) USA300HOU_1157non‑specific serine/threonine protein kinase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base C (36/39);  minor base T (1/0);  total (37/40)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAAGATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCAT  >  CP000730/1232330‑1232599
                                                                                                                                       |                                                                                                                                      
gaagatactttttACTACTTAGAAATGAAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACATAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCa                                                                                                                                    <  1:17226/129‑1 (MQ=255)
gaaaaTGACGCTTACTACTCAATAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTGTACGAATCAAATATTGGATGGCATTAAACATCCGCa                                                                                                                                    <  2:294356/140‑1 (MQ=255)
      gACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTCTATTAAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGAATGGCATTTAACATTCGCATGatat                                                                                                                              >  1:421584/1‑140 (MQ=255)
      gACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTTGATGGAATTAAACTTCCGCATGataa                                                                                                                              >  1:211761/1‑139 (MQ=255)
       aCTGTTACTACTTAGTAATGGCATACATCAAAGGTCCGACTTTGTCAGAGTATATTCAAGGTCATGGGCCATTAATTTTTGACACATCGATTAATTTGACTAATCAAATATTGGATGGCATTAAACATCCGCATGATATg                                                                                                                             <  2:242872/140‑1 (MQ=255)
       aCTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATg                                                                                                                             >  1:279095/1‑140 (MQ=255)
            tactacTTAGTAATGGAATATATCGAAGCTCCGACTTTGTCTGAGTATATTGAATGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTAt                                                                                                                        >  1:469932/1‑140 (MQ=255)
            tactacTTAGTAAAGGAATATATCGAAGGAAAGACTTTACAAGCCAACAATAAAAGTCAGGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTAt                                                                                                                        >  2:512718/1‑140 (MQ=255)
              ctactTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTg                                                                                                                      <  2:573974/140‑1 (MQ=255)
              ctactTAGAAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTg                                                                                                                      <  1:695855/140‑1 (MQ=255)
                atttAGTAATGGAATATATCGAAAGTCCGACTTAGTCTGAGTATATTGGAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTa                                                                                                                    <  1:380261/138‑1 (MQ=255)
                actTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTa                                                                                                                    <  2:67385/140‑1 (MQ=255)
                    aGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTGCaga                                                                                                                >  2:576305/1‑138 (MQ=255)
                    aGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATa                                                                                                                >  1:570747/1‑140 (MQ=255)
                     ggaaTGGAATATATCGAAAGTCCCACTTTGTCTAAGTATATGGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTGTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATag                                                                                                               <  2:173653/138‑1 (MQ=255)
                     gTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATag                                                                                                               <  1:418322/140‑1 (MQ=255)
                     gTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATag                                                                                                               <  1:609257/140‑1 (MQ=255)
                      tAATGGAAGATATCGAAGGTCCTACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTCGATGGCATTAAACATCCGCATTATATGCGTATTGTACATaga                                                                                                              <  2:107910/140‑1 (MQ=255)
                        aTGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAgaga                                                                                                            <  1:599612/140‑1 (MQ=255)
                              tatatCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCACATGATAATCGTAAGGGACATAGAGATATTaa                                                                                                      >  1:674360/1‑140 (MQ=255)
                              tatatCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTGCACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTaa                                                                                                      >  1:358047/1‑140 (MQ=255)
                               atatCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAg                                                                                                     >  2:63831/1‑140 (MQ=255)
                                  tCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCca                                                                                                  >  2:714976/1‑140 (MQ=255)
                                  tCGAAGGTCCCACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCca                                                                                                  <  1:295142/140‑1 (MQ=255)
                                    gAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGGGATATTAAGCcaca                                                                                                >  2:19383/1‑140 (MQ=255)
                                    gAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCcaca                                                                                                >  1:321412/1‑140 (MQ=255)
                                    gAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCcaca                                                                                                >  1:321497/1‑140 (MQ=255)
                                     aaGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACaa                                                                                               >  2:32224/1‑140 (MQ=255)
                                      aGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATAAATTGGTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACaaa                                                                                              >  2:477427/1‑140 (MQ=255)
                                       ggTCCGACTTTGTCTGAGTGCAGTGAAAGTCAGGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACaaaa                                                                                             <  2:507226/140‑1 (MQ=255)
                                        gTCCGACTTTGTCTGAGTATATTGAATGTCATGGGCCGTTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCTTGATATGCGTTTTGTACATAGAGATATTAAGCCACAAAat                                                                                            <  1:282917/140‑1 (MQ=255)
                                        gTCCGACTTAGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATGAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAat                                                                                            <  1:310292/140‑1 (MQ=255)
                                          ccGACTTTGTCTGGGAATATTGAAAGGCGTGGGCCATGAAGTATTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatat                                                                                          >  2:425407/1‑140 (MQ=255)
                                          ccGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatat                                                                                          >  1:178021/1‑140 (MQ=255)
                                          ccGACTTGGTCTGAGTATATTGAAAGTCATGGGCAATTAAGTGTTGACAGAGAGATAAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatat                                                                                          <  1:20276/140‑1 (MQ=255)
                                           cGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAACCATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACCAActata                                                                                         >  2:29544/1‑140 (MQ=255)
                                           cGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatata                                                                                         >  2:284923/1‑140 (MQ=255)
                                            gACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTTCTCTCGCGAAGCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatatat                                                                                        <  1:167395/140‑1 (MQ=255)
                                             aCTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATAtt                                                                                       <  1:661457/140‑1 (MQ=255)
                                                 tGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAAtt                                                                                   >  1:255866/1‑140 (MQ=255)
                                                  gTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCAACAAAATATATTAATTg                                                                                  >  1:299958/1‑140 (MQ=255)
                                                           tatTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATa                                                                         >  1:344643/1‑140 (MQ=255)
                                                            atTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATAGGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCCATaa                                                                        >  2:324561/1‑140 (MQ=255)
                                                             ttGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGAAGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATAAATTAATTGACAGCAATaaa                                                                       >  2:233338/1‑140 (MQ=255)
                                                                      aTGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGaaa                                                              >  1:3876/1‑140 (MQ=255)
                                                                      aTGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAAAAATAATAAGCAAAAAAATAAATAAAAAAACAGCAATAAAACGATGaaa                                                              >  1:286956/1‑140 (MQ=255)
                                                                         ggCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTGCTCGACAAAGAGATCTTAAGCCACAAAATATATTAATTGACAGCAATAAAAAGTTGAAAata                                                           >  1:346668/1‑140 (MQ=255)
                                                                          gCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACACAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAatat                                                          <  2:375329/140‑1 (MQ=255)
                                                                            cATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATAttt                                                        <  1:375942/140‑1 (MQ=255)
                                                                               tAAGTTTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGAt                                                     <  1:578334/140‑1 (MQ=255)
                                                                                 aGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGAttt                                                   <  2:55451/140‑1 (MQ=255)
                                                                                 aGTGTTGACACAGCGATAAATTTTACGAATCAAATATTGGATGGCATCAAACATCGGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGAttt                                                   <  1:536164/140‑1 (MQ=255)
                                                                                     ttGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGTGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGa                                               <  1:477427/140‑1 (MQ=255)
                                                                                       gACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAAt                                             <  2:644461/140‑1 (MQ=255)
                                                                                            aGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTa                                        <  2:279095/140‑1 (MQ=255)
                                                                                             gCGATTATTTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTaa                                       <  2:393902/140‑1 (MQ=255)
                                                                                             gCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATCGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAACATATTTGATTTTGGAATTGCTaa                                       <  2:519991/140‑1 (MQ=255)
                                                                                             gCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACAACCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTCAAAATATTTGATTTTGGAATTGCTaa                                       <  2:435800/140‑1 (MQ=255)
                                                                                               gATGAATATTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGGGTAGTGGACAGAGAGACATTAACCCACAAAATATATTAATTGACATCAAGAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAg                                     <  1:166255/140‑1 (MQ=255)
                                                                                                aTTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGc                                    >  1:623105/1‑140 (MQ=255)
                                                                                                   aaTTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCttt                                 <  2:623347/140‑1 (MQ=255)
                                                                                                   aaTTTTACGAATCAAATATTGGATGGCATTAAACATCCGCACGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCttt                                 >  2:124701/1‑140 (MQ=255)
                                                                                                        tACGAATCAAATATTGGATGGCATAAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTg                            <  1:183470/140‑1 (MQ=255)
                                                                                                          cGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTgag                          <  1:63831/140‑1 (MQ=255)
                                                                                                            aaTCAAATATTGGAAGGAAATAAATACCCGAATGATATGCGTATTAGACATAGAGATAGTAAGCCACAAAATATATTAATTGACAGCAATAAGACGTTGTAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGAc                        <  1:233338/140‑1 (MQ=255)
                                                                                                              tCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTGTGGAATTGCTAAAGCTTTAAGTGAGACGt                      >  1:222077/1‑140 (MQ=255)
                                                                                                               cAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTc                     >  1:325681/1‑140 (MQ=255)
                                                                                                               cAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTc                     <  1:721499/140‑1 (MQ=255)
                                                                                                                 aaTATTGGATGGCATTAAATATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCtt                   <  1:337845/140‑1 (MQ=255)
                                                                                                                  atatTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATAGCTAAAGCATAAAGTGAGACGTCttt                  >  1:80365/1‑140 (MQ=255)
                                                                                                                  atatTGGATGGCATTAAACATCCGCATGATATGAGTAGTGTACGTCGAGATATTAAGCCACAACATATATTAATTGACAGCAATAAAACGTTGAAAAGATTTGATATTGGAATTGCAACAGCTTTAAGTGAGACGTCtct                  >  1:283358/1‑138 (MQ=255)
                                                                                                                      tGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGCCGTCTTTAACt              >  2:104125/1‑140 (MQ=255)
                                                                                                                              aTTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACCAAATATGTTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTaa      >  1:21404/1‑140 (MQ=255)
                                                                                                                                 aaaCTTCCGCATGATATGCATAATGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGAAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCa   <  1:6022/140‑1 (MQ=255)
                                                                                                                                  aaCATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTAAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGGCTTTAACTCAGACGAATCAt  >  2:545297/1‑140 (MQ=255)
                                                                                                                                  aaCATCCGCATGATATGCGTATTGTAAATAGAGATATTAAGCCACAAAATATATTAATTGAAAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCAt  <  1:185821/140‑1 (MQ=255)
                                                                                                                                  aaCATCCCCATGATATGCTTATTGTACATAGAGATATCAATCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCAt  <  2:37858/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
GAAGATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCAT  >  CP000730/1232330‑1232599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: