Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,416,4580AT100.0% 142.2 / NA 46I216N (ATT→AAT) USA300HOU_1314oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (23/23);  total (23/23)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTT  >  CP000730/1416324‑1416593
                                                                                                                                      |                                                                                                                                       
tAACTCAATCATAGTAATCCCTCTTTAATTGTTTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTAc                                                                                                                                    <  1:273907/140‑1 (MQ=255)
           tAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGCTGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATAATTTGACCTTCTTTTAAAACAAAATTTTACTAACAATActt                                                                                                                         >  1:257382/1‑140 (MQ=255)
             gTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCa                                                                                                                       >  1:22299/1‑140 (MQ=255)
             gTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGAAAATGATGACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCa                                                                                                                       >  1:346489/1‑140 (MQ=255)
             gTAATCCCTCTTTAATTGTGTTATCTATTTAATTAGACATTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCa                                                                                                                       >  1:466700/1‑140 (MQ=255)
               aaTCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTAATGACAAAATTTCAAt                                                                                                                     >  1:305440/1‑140 (MQ=255)
               aaTCCCTCTTTAATTGTGTTATATATATAATTAAACGATCAGTATTCGGATGCCAATGCTCTTACTTGCTATCCTTAGTATTGCCGCGTTCAATGATTTGACATTCTTTTAAAACATAATTCTAATGACAATATTTCatt                                                                                                                     >  2:291492/1‑138 (MQ=255)
                   ccTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATCATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACAt                                                                                                                 >  2:313950/1‑140 (MQ=255)
                    ctctTTAATTGTTTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATg                                                                                                                <  2:436978/140‑1 (MQ=255)
                       ttttATTGTGTTCTATATTTAATTTGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAACGTTACCCCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACt                                                                                                             <  1:143836/140‑1 (MQ=255)
                                gtTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtg                                                                                                    <  2:664608/140‑1 (MQ=255)
                                    tataTTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgtgtg                                                                                                >  1:183472/1‑140 (MQ=255)
                                     atatTTAATTAAACATATAGAATACGGATGCAAATGCTCATACTTGAAATGATTAATAATACCTCATTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGa                                                                                               <  1:639533/140‑1 (MQ=255)
                                             ttAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAAAATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATaataaat                                                                                       <  2:441524/140‑1 (MQ=255)
                                                gACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAaataat                                                                                    <  1:409908/140‑1 (MQ=255)
                                                 aCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATg                                                                                   >  1:608802/1‑140 (MQ=255)
                                                 aCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATg                                                                                   >  2:48067/1‑140 (MQ=255)
                                                     tCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATCTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                                                               <  1:375506/140‑1 (MQ=255)
                                                       aGTATACGGGTGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATTCATGACTTCAGTTATGTGTTATTATAAATTATGTTTGAc                                                                             <  2:231559/140‑1 (MQ=255)
                                                        gTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAcc                                                                            >  2:321307/1‑140 (MQ=255)
                                                         tatctggATTCAAATGCCCATATTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTGTAAAACATAATTGGACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCa                                                                           <  1:305644/135‑1 (MQ=255)
                                                         tataCGGATTCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCa                                                                           <  1:416209/140‑1 (MQ=255)
                                                         tataCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCa                                                                           <  1:431303/140‑1 (MQ=255)
                                                         tataCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTGATGAGTGATAATAAATAATGTTTGACCa                                                                           >  2:296878/1‑140 (MQ=255)
                                                                 tGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTTAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTaa                                                                   <  1:212207/140‑1 (MQ=255)
                                                                    aaaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATAc                                                                >  1:316853/1‑140 (MQ=255)
                                                                     aaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAGTAAATAATGTTTGACCATGTTCTAATACa                                                               >  1:322054/1‑140 (MQ=255)
                                                                      acgCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTAACCATGTTCTAATACaa                                                              >  1:650570/3‑140 (MQ=255)
                                                                           cATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGc                                                         <  1:224368/140‑1 (MQ=255)
                                                                             tACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAAAACAATATGCTg                                                       >  2:321979/1‑140 (MQ=255)
                                                                                   aaaTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGtaataa                                                 >  1:323740/1‑140 (MQ=255)
                                                                                        aTTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCATTACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCa                                            >  2:395291/1‑140 (MQ=255)
                                                                                            atatTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCAc                                        >  2:314082/1‑140 (MQ=255)
                                                                                            atatTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCAc                                        <  1:474210/140‑1 (MQ=255)
                                                                                                tACCTCGTTCAATGATTCGACCTTCTTTTAAAACATAATTGTACTGACAATATTCCAATACATGACTTAAGTAATGTGTGATAAAAAATAATGTTTGACCATCTTCTAATACAATATGCTGTAATAACTCCATCACTTGa                                    >  2:499976/1‑140 (MQ=255)
                                                                                                   cTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTa                                 <  1:48067/140‑1 (MQ=255)
                                                                                                        tCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGtt                            <  1:382451/140‑1 (MQ=255)
                                                                                                        tCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGtt                            >  1:38682/1‑140 (MQ=255)
                                                                                                            tagattGTCCTTCTTTTAAAACATAATTGGACTGACAATATTTCAATACACAACTTAAGTTATGTGTGATAATAAATAATGTTTGACCCTGTCCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCaaa                        <  2:83908/136‑1 (MQ=255)
                                                                                                             gATTTGAACTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTAAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATTGGATGTAATAAATCCATCACTTGTTTCGCGTTCAAAg                       >  2:304346/1‑140 (MQ=255)
                                                                                                                ttGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACGTGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATAGGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCAt                    <  2:346489/140‑1 (MQ=255)
                                                                                                                ttGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCAt                    <  2:22299/140‑1 (MQ=255)
                                                                                                                             aaaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAAc       <  2:323740/140‑1 (MQ=255)
                                                                                                                                aCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTgg    <  1:74554/140‑1 (MQ=255)
                                                                                                                                 cATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATACGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGt   >  2:489917/1‑140 (MQ=255)
                                                                                                                                  aTAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATATATCCATCACTTGATTACCGTTCAAAGCATCCAATGATTCAACTGGtt  <  2:466700/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                       
TAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTT  >  CP000730/1416324‑1416593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑