Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,646,1890GC100.0% 170.1 / ‑6.5 53A155G (GCA→GGA) USA300HOU_1531M24B family Xaa‑Pro dipeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (25/26);  minor base A (1/0);  total (26/27)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATAC  >  CP000730/1646054‑1646324
                                                                                                                                       |                                                                                                                                       
tgaTCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCTATCTGCTCCTAAGTCTAGCATTTTGCTTTCTAATATCGCCTTTAATTCTTTTTCAGTCATGCCTCCttt                                                                                                                                     <  1:154382/138‑1 (MQ=255)
  aTCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTACCTTTAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                   >  1:371754/1‑140 (MQ=255)
  aTCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGAAGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATCCCTCCTTTTa                                                                                                                                   >  2:633986/1‑140 (MQ=255)
    cACTTGCAACACCATGTTGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTTTATTATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTaca                                                                                                                                 <  1:190356/140‑1 (MQ=255)
     ccTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaa                                                                                                                                <  1:576425/139‑1 (MQ=255)
     aCTTGCAACACCATGTGGTAATGCACCTCTATGACCAGCTGCTACACTCGTATCGAATGATGGTCAATCTGCTCCTAATTCTAGCATTTTGCTTTATAGTCTTGCCTTTAATTCTTTTTGAGTCGTGCCTAATTTTacaa                                                                                                                                >  2:612029/1‑140 (MQ=255)
         gCAACACCATGTGGTAATGCACCTCTATGACCAGATGTTATAATCGTATCGAATGATGGTTCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGt                                                                                                                            <  1:17946/140‑1 (MQ=255)
         gCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGt                                                                                                                            <  1:488842/140‑1 (MQ=255)
         gCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGt                                                                                                                            <  1:431414/140‑1 (MQ=255)
         gCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGt                                                                                                                            <  1:618886/140‑1 (MQ=255)
           aaCACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTa                                                                                                                          <  2:6202/140‑1 (MQ=255)
            acacCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACATTTaa                                                                                                                         >  2:437510/1‑140 (MQ=255)
                     ggTAATTCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGATTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCGCCTTTTACAACAGTTAAAATATATTc                                                                                                                >  2:327762/1‑140 (MQ=255)
                     ggTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTc                                                                                                                >  2:559389/1‑140 (MQ=255)
                      gTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCa                                                                                                               <  2:446834/140‑1 (MQ=255)
                        aaTGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCata                                                                                                             >  1:606864/1‑140 (MQ=255)
                         aTGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATTCCTCCTTTTACAACAGTTAAAATATATTCatat                                                                                                            >  2:230850/1‑140 (MQ=255)
                          tGCACCTCTATGACCAGATGCTACAATCGTAGCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATg                                                                                                           <  2:408445/140‑1 (MQ=255)
                             aCCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGttt                                                                                                        >  2:654992/1‑140 (MQ=255)
                             aCCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCATCATACTAGCATTTTGCTTTCTAATATTGCCTTTAATTATTTCTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCCTATGttt                                                                                                        >  2:200738/1‑140 (MQ=255)
                                  tATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcatca                                                                                                   >  2:524328/1‑140 (MQ=255)
                                  tATGACCAGATGCTACAAGCGTATCGAATGATGGTCCAGCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcatca                                                                                                   <  2:630508/140‑1 (MQ=255)
                                        cAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAata                                                                                             >  1:259341/1‑140 (MQ=255)
                                          gATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATAtt                                                                                           >  1:645122/1‑140 (MQ=255)
                                              cTACAATCGTATCGAATAATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgct                                                                                       >  2:88864/1‑140 (MQ=255)
                                                 cAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctgct                                                                                    >  1:124596/1‑140 (MQ=255)
                                                 cAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctgct                                                                                    >  2:726666/1‑140 (MQ=255)
                                                                       aTCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGt                                                              <  1:717313/140‑1 (MQ=255)
                                                                          tGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAg                                                           >  1:54507/1‑140 (MQ=255)
                                                                           gCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGc                                                          <  2:68569/140‑1 (MQ=255)
                                                                            cTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAATTTATATTCATATGTTTCATCAACAATATTATCTACTTTTTGAAGTAAAGCAATTTCGTCCGCa                                                         >  1:672461/1‑140 (MQ=255)
                                                                                      tAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTTACGt                                               <  1:495827/140‑1 (MQ=255)
                                                                                       aGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTAAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtc                                              <  1:489133/140‑1 (MQ=255)
                                                                                        gCATTTTGCTTTCTAATATTGACTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtct                                             >  1:376195/1‑140 (MQ=255)
                                                                                         cATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                                            >  1:391482/1‑140 (MQ=255)
                                                                                         cATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCCTTTCCAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTCTTGAATTAAAGCAATTTCGTCAACATCTTTTACGtctc                                            >  1:39963/1‑140 (MQ=255)
                                                                                          aTTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTctct                                           <  1:29723/140‑1 (MQ=255)
                                                                                             ttGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAt                                        <  2:258267/140‑1 (MQ=255)
                                                                                                 tttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACATTTAAAATATATTCATATGTTTCATCGACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCTGCATCTTTGTCGTTTCTAATTTTa                                    >  2:121678/1‑140 (MQ=255)
                                                                                                               tttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGa                      <  1:98829/140‑1 (MQ=255)
                                                                                                                ttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTCAAGCAATTTCGTCAGCCTCTTTGACGTCTCTAATTTTATCTACAGTATTAGaa                     >  1:692212/1‑140 (MQ=255)
                                                                                                                    ttCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGc                 <  1:230850/140‑1 (MQ=255)
                                                                                                                     tgggtttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCt                <  2:725106/136‑1 (MQ=255)
                                                                                                                     tCTTTTTCAGTCATGCCTTCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCt                <  2:340104/140‑1 (MQ=255)
                                                                                                                     tCTTTGTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGTAATTTCGTCAGCATCTTTGAGGGGTTTAATTTTATCTCCAGTATTAGAAATGCt                <  2:480893/140‑1 (MQ=255)
                                                                                                                      cTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtt               >  1:267315/1‑140 (MQ=255)
                                                                                                                       tttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtta              <  2:222009/140‑1 (MQ=255)
                                                                                                                        ttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttat             >  1:344228/1‑140 (MQ=255)
                                                                                                                              gTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGa       <  1:726666/140‑1 (MQ=255)
                                                                                                                              gTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGa       <  2:124596/140‑1 (MQ=255)
                                                                                                                                 aTGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGatat    <  2:195824/140‑1 (MQ=255)
                                                                                                                                  tGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGAtata   >  1:706208/1‑140 (MQ=255)
                                                                                                                                   tcctcctTTTACAACTGTTAAAATATATTCATATGTTTCCCCACGAATATTAGCTGCTTTTTGCATGAAAGCAATTTCGTCAGCATCTTTGGCGTCTCTAATTTTAGCTACAGTATTAGAAATGCTTATTAATGATATAc  <  2:531811/139‑1 (MQ=255)
                                                                                                                                   gCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCTGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATAc  <  1:681965/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATAC  >  CP000730/1646054‑1646324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑