Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 110,660 T→A 16.5% I173I (ATT→ATA USA300HOU_0109 → staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730110,6600TA16.5% 165.6 / 23.9 72I173I (ATT→ATAUSA300HOU_0109staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (26/33);  new base A (8/4);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.48e-01

GTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGA  >  CP000730/110523‑110796
                                                                                                                                         |                                                                                                                                        
gTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAAAATAGCGACTTTAAAAATTTGACAAAATATAAAGACGGAGATATTTc                                                                                                                                        >  2:131413/1‑140 (MQ=255)
  tAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCat                                                                                                                                      >  1:28343/1‑140 (MQ=255)
   aaaaTGGTCGATAATAAAATCATTCCAACAAAATAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCata                                                                                                                                     <  1:598781/140‑1 (MQ=255)
    aactGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATCAAAAAAGAAATCGAAAACTCTAAGTTCTTCGTTCAATATCGCGACTTTAACAATTTGAAAACTTATAAAACCGGAGATATTTCctat                                                                                                                                    >  2:141774/4‑140 (MQ=17)
    aaaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCatat                                                                                                                                    <  1:645112/140‑1 (MQ=255)
      aTGGTCGATAATAAAATCATTCCAACAAAAGAAATCAGATATGAATAAATAAAAAAAGAAATCGAAAACTTTACGTGCGTTGTTCAATATGCAGACTTTGAAAATGGCAAAAATGATAAAAAAGGGGACAtttaaaaaaa                                                                                                                                  >  2:546563/1‑133 (MQ=9)
       tGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGATATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTCTAAAGACGGAGATATTTCATATAAt                                                                                                                                 >  1:439229/1‑140 (MQ=255)
       tGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAAt                                                                                                                                 >  2:610214/1‑140 (MQ=255)
        ggTCGATAATAAAATCATTCCAACAAAAGNAATTAAAGATGNAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGCCGACTTTTAAAATTTGAAAAAGTACAAAAACGGAGATATGTCANATaagc                                                                                                                                >  2:17522/1‑138 (MQ=17)
            aataataAAATCATTCCAACACGAGAAAAAAAAGATGAAAAAATAAAAAAAGAAATCTAAAACTTGAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCaga                                                                                                                            <  1:194774/139‑1 (MQ=17)
             ataataAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATCTGGCGCCTATAAACGTTTGCCCAATGATAAAGACGGAGATATTTCATATAATCCagag                                                                                                                           <  1:350002/140‑1 (MQ=14)
             ataataAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCagag                                                                                                                           <  1:469277/140‑1 (MQ=255)
              taataaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAgg                                                                                                                          <  1:611363/140‑1 (MQ=255)
                  aaaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATACAAAAAGAAATCGAACACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                      >  1:141113/1‑140 (MQ=255)
                     aTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAAATATAAAGACGGAGATATTTCATATAATcacgagtagaccaa                                                                                                                   >  2:610984/1‑127 (MQ=17)
                           ccAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAAAGGTGCACAGGTATTc                                                                                                             >  2:432240/1‑140 (MQ=255)
                             aacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGCAAACTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCgg                                                                                                           <  2:476326/140‑1 (MQ=255)
                                   aGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                     <  1:436556/140‑1 (MQ=255)
                                     aaaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAatat                                                                                                   <  2:353231/140‑1 (MQ=255)
                                       aTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAAACGGAGATATTTCATATAATCCAGAGATGCCGAGTTATTCGGCTAAATAACa                                                                                                 >  2:260746/1‑140 (MQ=255)
                                       aTTAAAGATAAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTATTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGATGCCGAGTTATTCGGCTAAATATCa                                                                                                 >  2:338750/1‑140 (MQ=255)
                                          aaaGATGAAAAAATAACAAGAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                              >  1:405838/1‑140 (MQ=255)
                                          aaaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                              >  1:552465/1‑140 (MQ=255)
                                              aTGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                          <  1:703166/140‑1 (MQ=255)
                                                 aaaaaaTAAAAAAAGAAATCGAAAAATTCAAGTTCTTCATTCAATAGTGAGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAat                                                                                       <  1:561128/140‑1 (MQ=14)
                                                   aaaaaaaaaaATNAAAAGAAAAACTTTAANTAATTTGTTCAATATTGCNACATTAAAAATTTAAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCGGAGTTATTCGGCTAAATATCAATTAACTAatga                                                                                     <  1:17522/140‑1 (MQ=14)
                                                      aTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                                  <  2:631985/140‑1 (MQ=255)
                                                           aaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAATTTTTAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtata                                                                             <  1:258139/140‑1 (MQ=255)
                                                                 aaTCGAAAACTTTAAGTTCTTTGTTCAATATTGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                       <  2:433052/140‑1 (MQ=255)
                                                                        aaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATAAATTAACTAATGATGGTTATAATGTAAAACAAtt                                                                >  2:116802/1‑140 (MQ=255)
                                                                          cTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                              <  2:28343/140‑1 (MQ=255)
                                                                           ctttaGTTCTTTGTACAACATGGCGACTTTAAACATTTGAAAAATGATAAAGACGGAGATAGTTCATATAATCCAGAGGTGCCCACCTATTCGGCTAAATATCAACTACCTATTGATGATTATAATGTAAAACAATTACg                                                             <  2:442362/136‑1 (MQ=17)
                                                                            ttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                            >  1:661565/1‑140 (MQ=255)
                                                                            ttAAGTGCTGTGTGGAAAGTGGCCAGTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                            <  2:604233/140‑1 (MQ=17)
                                                                             tAAGTTCTTTGTTCAATATTGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCa                                                           <  2:664099/140‑1 (MQ=255)
                                                                             tAAGTTCTTCGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCa                                                           <  1:10695/140‑1 (MQ=255)
                                                                             tAAGTACTTTGTTCAATATAGCGACTTTAAAAATTTTAAAAATTATAAATATGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATGCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCa                                                           <  1:10763/140‑1 (MQ=17)
                                                                              aaGTTCTTTGTTCAATATGGCTACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa                                                          <  2:557728/140‑1 (MQ=255)
                                                                               aGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAAGCaaa                                                         >  2:722130/1‑140 (MQ=255)
                                                                               aGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATAACAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaa                                                         >  2:256915/1‑140 (MQ=255)
                                                                                   cTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgat                                                     <  1:603487/140‑1 (MQ=255)
                                                                                   cTTTGTTCAATATGGCGACGTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgat                                                     <  1:216365/140‑1 (MQ=255)
                                                                                         tCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                               <  1:187970/140‑1 (MQ=255)
                                                                                         tCAATATGGAGATTTCAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGAAGAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                               <  1:20988/140‑1 (MQ=17)
                                                                                                    aCTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATAAACCGACGAGt                                    >  2:575040/1‑140 (MQ=255)
                                                                                                      tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                                  <  2:595658/140‑1 (MQ=11)
                                                                                                      tttAAAAATTTGAAAAATTATAAAGACGGATATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                                  <  1:95532/140‑1 (MQ=255)
                                                                                                        tAAAAATTTGAAAAATTATAAAGACGGAGATAGTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAGTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAg                                <  2:405838/140‑1 (MQ=255)
                                                                                                         aaagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGGGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTAAAATGAAAAACAATTACGCAAAAGATATGATATACCGACGAGAAAAGc                               >  1:256829/13‑140 (MQ=11)
                                                                                                         aaagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                               <  1:397488/128‑1 (MQ=11)
                                                                                                         aaaaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                               >  1:99895/1‑140 (MQ=255)
                                                                                                         aaaaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTATGCTAAATATCAATTAACTAATAATGATTATAATGTACAACAGTTACGCAAAAGATATGATCTACCGACAACTAAAGc                               >  2:171744/1‑140 (MQ=17)
                                                                                                         aaaaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATACAGAGGTGCCGAGTTATTTGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAAAATTAAGCAAGAGGTAAGTAATACCGACGAGTAAAgt                               >  1:14434/1‑139 (MQ=18)
                                                                                                          aagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                              >  2:227635/12‑140 (MQ=11)
                                                                                                           agatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAAAGTAAAACAATTAAGCAAAAAGTATGATATACAGACGAGAAAAGCTc                             >  1:188842/11‑140 (MQ=11)
                                                                                                            gatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCGCTTGACTGATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                            >  1:168002/10‑140 (MQ=11)
                                                                                                            gatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAGGAAAAAGATATGATATAACGAAGAGTAAAGCTcc                            >  1:216453/10‑140 (MQ=11)
                                                                                                            gatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGccac                            >  2:446556/10‑137 (MQ=11)
                                                                                                            gatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                            <  1:434495/131‑1 (MQ=14)
                                                                                                            aaTTTTAAAAAGTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                            <  2:389372/140‑1 (MQ=255)
                                                                                                            aaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGGGGTGCCGAGTTATTCGGCTAAATACCAAGTAACTAATGATGATTATAATGTAAAACAATTACGCAACAGATATGATATACCGACGAGTAAAGCTcc                            <  2:269228/140‑1 (MQ=255)
                                                                                                            aaTTTAAAAAATTAAAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTACTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                            <  1:131225/140‑1 (MQ=255)
                                                                                                               ttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaaa                         >  1:10908/1‑140 (MQ=255)
                                                                                                                 aatgaaTTATAAAGATGGAGACATATCATAAAATCCAGAGGGGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGt                       <  2:243777/136‑1 (MQ=17)
                                                                                                                  atgaaTTATAAAGATGAAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGAAAAGCTCCAAAGtt                      >  1:432976/4‑140 (MQ=14)
                                                                                                                   aaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCTAAGTTa                     >  2:524688/1‑140 (MQ=255)
                                                                                                                      aTTATAAAGACGGAGATATTTCATATAATCAAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATTCAAACGAGTAAAGCTCCaacaatctaa                  >  2:724624/1‑132 (MQ=17)
                                                                                                                           aaaGATGGAGCTATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa             >  2:492527/1‑140 (MQ=255)
                                                                                                                                 ggggaTATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAGTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGCTATTGTTAAAAGGTTc       <  2:439229/137‑1 (MQ=255)
                                                                                                                                 ggAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTc       <  1:610214/140‑1 (MQ=255)
                                                                                                                                 ggAGATATTTAATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTATAAGGTTc       >  2:703309/1‑140 (MQ=255)
                                                                                                                                  gaaatatatCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGATATACCGACGAGTCAATCTCCAAAGTTATTGTTAAAAGGTTCa      <  2:424402/137‑1 (MQ=17)
                                                                                                                                      tatTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAgggc  >  2:429218/1‑139 (MQ=255)
                                                                                                                                      tatTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAgagg  >  2:706894/1‑137 (MQ=255)
                                                                                                                                         |                                                                                                                                        
GTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGA  >  CP000730/110523‑110796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: