Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,784,962 C→T 5.0% G40D (GGC→GAC)  thrS ← threonine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,784,9620CT 5.0% 235.9 / 2.6 80G40D (GGC→GAC) thrSthreonine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (38/37);  new base T (2/2);  total (41/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

ATCGCGTGTGCCATTAAATGTGCAGTAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATATTAATTTGTTCCATGTTAAACCCTCCTATTTGCTT  >  CP000730/1784827‑1785101
                                                                                                                                       |                                                                                                                                           
aTCGCGTGTGCCATTAAATGTGCAGTAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGTCGGc                                                                                                                                         >  1:655294/1‑140 (MQ=255)
aTCGCGTGTGCCATTAAATGTGCAGTAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGTCGGc                                                                                                                                         >  1:610736/1‑140 (MQ=255)
 tCGCGTGTGCCATTAAATGTGCAGTAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGcc                                                                                                                                        >  2:450846/1‑139 (MQ=255)
 tCGCGTGTGCCATTAAATGTGCAGTAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCa                                                                                                                                        >  2:513452/1‑140 (MQ=255)
      tgtgCCATTAAATGTGCAGTAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGc                                                                                                                                   <  1:132554/140‑1 (MQ=255)
      tgtgCCATTAAATGTGCAGTAGAATGACCTAATACCTCTTACGCTTCTTCACTTCCTTGTGTCACAATTTCAATTGATCCATCAGTTTCAATCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGc                                                                                                                                   <  1:322903/140‑1 (MQ=255)
       gtgCCATTAAATGTGCAGTAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCt                                                                                                                                  <  2:572592/140‑1 (MQ=255)
         gCCATTAAATGTGCAGTAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCttt                                                                                                                                >  2:405177/1‑140 (MQ=255)
          ccATTAAATGTGCAGTAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACCATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCACGTTAAATTTGCCGGCAACAGCtttt                                                                                                                               >  1:7691/1‑140 (MQ=255)
           cATTAAATGTGCAGTAGAATGACGTAATACCTTTATCGCTTCTTCACTACCTGTTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCttttt                                                                                                                              <  1:134066/140‑1 (MQ=255)
              tAAATGTGCAGTAGCATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTAc                                                                                                                           <  2:654712/140‑1 (MQ=255)
              tAAATCTGCAGTAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTAc                                                                                                                           <  2:577996/140‑1 (MQ=255)
                 aggtgCAGTAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTa                                                                                                                        <  2:680679/138‑1 (MQ=255)
                 aTGTGCAGTAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAGGCCCGGTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTa                                                                                                                        >  1:345725/1‑140 (MQ=255)
                   gtgCAGTAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAAt                                                                                                                      >  1:200022/1‑140 (MQ=255)
                   gtgCAGTAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAAt                                                                                                                      >  2:462463/1‑140 (MQ=255)
                   gtgCAGTAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAAt                                                                                                                      <  2:121448/140‑1 (MQ=255)
                     gCAGTAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAATTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATcc                                                                                                                    >  1:492785/1‑140 (MQ=255)
                         tAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAAATGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGa                                                                                                                >  1:483936/1‑140 (MQ=255)
                         tAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTAAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAGTATACAAGTTGCCCGTTAAATCTGCCGGCAACAGCTTTTTTACGTAATCCAGGa                                                                                                                >  2:400311/1‑140 (MQ=255)
                         tAGAATGACGTAATACCTCTAACGCTTATTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTAAGTAATCCaagc                                                                                                                >  2:646885/1‑137 (MQ=255)
                           gAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTACATCTACAAGTTGCACGTTAAATTTGACGGCAAAAGCTTTTTTACTTAATCCAAGACt                                                                                                              >  1:642196/1‑140 (MQ=255)
                              tGACTTAATACCTCTATCGCTTCTTCTCTGCCTGGTGTCACAATTTCAATTGATCCATCAGTTTCTAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAat                                                                                                           <  1:108972/140‑1 (MQ=255)
                              tGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAat                                                                                                           <  1:59794/140‑1 (MQ=255)
                              tGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAat                                                                                                           <  1:135918/140‑1 (MQ=255)
                              tGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAat                                                                                                           <  1:59692/140‑1 (MQ=255)
                               gACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAatt                                                                                                          >  1:248264/1‑140 (MQ=255)
                               gACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAatt                                                                                                          <  2:462588/140‑1 (MQ=255)
                               gACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAatt                                                                                                          >  1:276368/1‑140 (MQ=255)
                               gACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAATTGACCGTTAAATTTTCCGGCAACAGCTTTTTTACGTAATCCAGGAATAatt                                                                                                          >  1:588927/1‑140 (MQ=255)
                                    aaTACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAGTCTACACGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGGAATGCAGGACTActtgactg                                                                                                     >  2:384077/1‑140 (MQ=255)
                                     aTACCTCTAACGCTTCTGCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGTCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATtgt                                                                                                    <  1:174987/140‑1 (MQ=255)
                                      tatctctAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATtgtg                                                                                                   <  2:177556/137‑1 (MQ=255)
                                      tACCTCTAACTCTTCGTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATtgtg                                                                                                   <  2:343276/140‑1 (MQ=255)
                                        ccTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCt                                                                                                 <  1:324631/140‑1 (MQ=255)
                                         ctctAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCta                                                                                                <  2:724311/140‑1 (MQ=255)
                                         ctctAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCta                                                                                                <  2:157085/140‑1 (MQ=255)
                                               cGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCCTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCtt                                                                                          <  2:663020/140‑1 (MQ=255)
                                                       cACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCagtagta                                                                                  >  2:523067/1‑140 (MQ=255)
                                                        aCTTCCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGTCCGTTAAATGTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCagtagtag                                                                                 <  1:408636/140‑1 (MQ=255)
                                                         cTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCagtagtagt                                                                                <  2:313974/140‑1 (MQ=255)
                                                              tGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCtt                                                                           >  2:717382/1‑140 (MQ=255)
                                                                gtgtCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTa                                                                         >  2:54214/1‑140 (MQ=255)
                                                                     acaATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCaaa                                                                    >  1:643546/1‑140 (MQ=255)
                                                                              aTTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCttttt                                                           >  1:263848/1‑140 (MQ=255)
                                                                              aTTGATCCATAAGTTTCAAGCGGTTTAGTTCGCTCTTCAAGTTGCCGTTTCAATTTGCCGCCATCAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACATTTATCAAACGCCttttt                                                           >  2:152760/1‑140 (MQ=255)
                                                                                 gATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAAAAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTttatt                                                        >  2:295593/1‑140 (MQ=255)
                                                                                   tCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTAc                                                      >  1:624526/1‑140 (MQ=255)
                                                                                      aTCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCAt                                                   <  1:298994/140‑1 (MQ=255)
                                                                                      aTCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGCCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCAt                                                   >  1:96987/1‑140 (MQ=255)
                                                                                         ggTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTg                                                <  2:248264/139‑1 (MQ=255)
                                                                                         aGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTg                                                <  1:667918/140‑1 (MQ=255)
                                                                                         aGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTg                                                <  2:276368/140‑1 (MQ=255)
                                                                                          gTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTgg                                               <  1:526489/140‑1 (MQ=255)
                                                                                          gTTTCAAGCGGTTTAGTTAAATCTACAAGTTGACCGTTAAATTTGCCGGCAAGACCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCATCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTgg                                               <  2:398592/140‑1 (MQ=255)
                                                                                               aaGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAAtt                                          >  1:84552/1‑140 (MQ=255)
                                                                                                     tttAGTTAAATCTACAAGTTGCCCGTTAAATTTGTCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAatat                                    <  2:610736/140‑1 (MQ=255)
                                                                                                     tttAGTTAAATCTACAAGTTGCCCGTTAAATTTGTCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAatat                                    <  2:655294/140‑1 (MQ=255)
                                                                                                      ttAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATAtt                                   >  1:331607/1‑140 (MQ=255)
                                                                                                       tAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACGTTTTTCAAACGCCCTTTTATTACCATCTGGAAATTAAATATTa                                  >  1:312793/1‑140 (MQ=255)
                                                                                                          ttAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATATTAAtt                               <  1:483395/140‑1 (MQ=255)
                                                                                                             aaTCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATATTAATTTGt                            <  1:660583/140‑1 (MQ=255)
                                                                                                             aaTCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTGTGTACGTCATCCAGGACTAATTGATTGTGCTATATCTTCAGTATTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGACTATTCAttttt                            <  2:267344/140‑3 (MQ=255)
                                                                                                               tCTACAAGGTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATATTAATTTGTTc                          <  1:298316/140‑1 (MQ=255)
                                                                                                                  aCAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATATTAATTTGTTCCAt                       >  1:44742/1‑140 (MQ=255)
                                                                                                                     aGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATATTAATTTGTTCCATGtt                    <  2:529680/140‑1 (MQ=255)
                                                                                                                      gTTGCCCGTTAAATTTGCCGGCAACAGTTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATATTAATTTGTTCCATGTTa                   <  1:615694/140‑1 (MQ=255)
                                                                                                                      gTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACTCCTTTTTTTTACCATCTGGAAATTGAATATTAATTTGTTCCATGTTa                   >  1:275375/1‑140 (MQ=255)
                                                                                                                      gTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACTATCTGGAAATTACATATTGATTTGTTCCATGTTa                   >  2:363726/1‑140 (MQ=255)
                                                                                                                      gTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATATTAATTTGTTCCATTTTa                   >  2:188808/1‑140 (MQ=255)
                                                                                                                      gTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATATTAATTTGTTCCATGTTa                   <  1:104794/140‑1 (MQ=255)
                                                                                                                      gTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATATTAATTTGTTCCATGTTa                   >  1:640298/1‑140 (MQ=255)
                                                                                                                      gTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAATACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATATTAATTTGTTCCATGTTa                   >  1:319864/1‑140 (MQ=255)
                                                                                                                       ttGCCCGTTAAATTTGCCGGCAACAGCTTTTTTCCGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATATTAATTTGTTCCATGTTaa                  <  2:603885/140‑1 (MQ=255)
                                                                                                                            cGTTAAATTTGCCGGCAACAGCTTTTTTTTTTTTTTTAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTCCCATCTGGAAATTGAATATTAATTTGTTCCATGTTAAACcct             >  2:110860/1‑140 (MQ=255)
                                                                                                                            cGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCCGAACGAATTGGTTTTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATATTAATTTGTTCCATGTTAAAcctt             >  2:363777/1‑138 (MQ=255)
                                                                                                                             gTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATATTAATTTGTTCCCTGTTAAACcctc            >  2:213804/1‑140 (MQ=255)
                                                                                                                              ttAAATTTGCCTGCAACATCTTTTTTACGTAATCCAGGACTAATTGATTTTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATATTAATTTGTTCCATGTTAAACcctcc           <  1:657927/140‑1 (MQ=255)
                                                                                                                                    ttGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATATTAATTTGTTCCATGTTAAACCCTCCTATTTg     >  2:107754/1‑140 (MQ=255)
                                                                                                                                    ttGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTCTTATTACCATCTGGAAATTGAATATTAATTTGTTCCATGTTAAACCCTCCTATTTg     >  1:717624/1‑140 (MQ=255)
                                                                                                                                       ccGGCAACAGCTTTTTTTCGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATATTAATTTGTTCCATGTTAAACCCTCCTATTTGCtt  <  2:36984/140‑1 (MQ=255)
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ATCGCGTGTGCCATTAAATGTGCAGTAGAATGACGTAATACCTCTAACGCTTCTTCACTACCTGGTGTCACAATTTCAATTGATCCATCAGTTTCAAGCGGTTTAGTTAAATCTACAAGTTGCCCGTTAAATTTGCCGGCAACAGCTTTTTTACGTAATCCAGGACTAATTGATTGTGCTATATCTTCAGTAGTAGTACCTTTATCAAACGCCTTTTTATTACCATCTGGAAATTGAATATTAATTTGTTCCATGTTAAACCCTCCTATTTGCTT  >  CP000730/1784827‑1785101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑