| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,802,628 | A→T | 7.8% | K321M (AAG→ATG) | USA300HOU_1683 → | APC family amino acid‑polyamine‑organocation transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,802,628 | 0 | A | T | 7.8% | 165.4 / 5.4 | 61 | K321M (AAG→ATG) | USA300HOU_1683 | APC family amino acid‑polyamine‑organocation transporter |
| Reads supporting (aligned to +/- strand): ref base A (28/27); new base T (2/3); total (30/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01 | |||||||||||
GGAATTCCGTTTGCTGCGGGCTTGATTAATTTTGTAGTATTAACCGCTGCTGCTTCATCATGTAACAGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTATTTACGTATGTGACTACCATCTCTACAGTGT > CP000730/1802489‑1802762 | ggAATTCCGTTTGCTGCGGGCTTGATTAATTTTTTAGTTTGTAGCGATGCTGACTTTGCATTTAGCTTTTTTTTATTCTCACATAGCCGTATGCTGTTCAGTTTATCAAGTCAACAACAAGGCCCGCCTACCTTTTCTaa > 2:512252/1‑140 (MQ=255) tgcGGGCTTGATTAATTTTGTAGTATTAACCGCTGCTGCTTCATCATGTAACAGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATg < 2:74194/140‑1 (MQ=255) cGGGCTTGATTAATTTTGTAGTATTAACCGCTGCTGCTTCATCATGTAACAGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGc < 1:254869/140‑1 (MQ=255) ggCTTGTTTAATTTTGTAGTATTACCCGCTGCTGCTTCATCATGTAACAGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCTCCTCCGAACTTTTCTAAGACGAATAAATATGGCGt < 2:86859/140‑1 (MQ=255) gtttGTTTAATTTTGTGGTATTAAGCGCTGCTGCTTCATCATGTAACAGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGtt < 2:125406/138‑1 (MQ=255) ttGATTAAGTTTCTAGTATTAACCGTTGCTGCGTCATCATGTAACAGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTAAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTcc < 2:356476/140‑1 (MQ=255) aTTAATTTTGTAGTATTAACCGCTGCTGCTTCATCATGTAACAGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCcaca < 1:293149/140‑1 (MQ=255) aTTAATTTTGTAGTATTAACCGCTGCTGCTTCATCATGTAACAGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCcaca > 1:458629/1‑140 (MQ=255) aaTTTGGTAGTATTAACCGCTGCTGCTTCATCATGTAACAGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGt > 2:275813/1‑140 (MQ=255) aTTTTGTAGTATTAACCGCTGCTGCTTCATCATGTAACAGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGtg > 2:652204/1‑139 (MQ=255) tAACCGCTGTTGCTTCATCATGTAACAGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCt < 2:702036/140‑1 (MQ=255) gctgctgctTCATCATGTAACAGTTGTATTTTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTtcatc < 1:41381/140‑1 (MQ=255) gctgctgccTCATCATGTAACTGTGGTTTATTCTCAATTAGGTGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTtcatc < 1:325899/140‑1 (MQ=255) gctgctTCATCATGTAACAGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGc < 1:671403/140‑1 (MQ=255) gcggctTCATCATGTAACAGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAAGAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAga > 2:574072/4‑139 (MQ=255) gctTCATCATGTAACAGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCatt < 2:627500/140‑1 (MQ=255) atgctGTAACAGTGGTATATTCTCAAATCGCTGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACAAATAAATATTGCGTTCCACATGTTCCAATCTCTGCTTCATCAGCATTATTAc < 2:245204/136‑1 (MQ=255) atGTAACAGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTtg < 1:351073/140‑1 (MQ=255) gTAACAGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTtgtg > 1:679397/1‑140 (MQ=255) gTAACAGTGGTATATACTCAAATTGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTtgtg > 2:701549/1‑140 (MQ=255) tAACAGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTgg < 2:689852/140‑1 (MQ=255) aggggTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGgcagc < 1:104615/137‑1 (MQ=255) aatGGTATATTCTCAAAAAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTTCTTCATCAGCATTATTACTTGTGgcagc < 1:461446/138‑1 (MQ=255) aGTGGTATATTCTCACATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGgcagc > 1:540836/1‑140 (MQ=255) aGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTATGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGgcagc > 2:560188/1‑140 (MQ=255) aGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGgcagc > 1:714958/1‑140 (MQ=255) aGTGGTATATTCTCAAATAGCAGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTATGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCGGCATTATTACTTGTGgcagc > 2:403373/1‑140 (MQ=255) tGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCAGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCAt < 1:375419/140‑1 (MQ=255) ctcAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTACAATCTTTGCTTCATCAGCATTATTCCTTATGGCAGCATTACTAAACt > 1:88205/1‑140 (MQ=255) cAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCTTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACAAAACtat > 2:230814/1‑140 (MQ=255) cAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTTGCAGCATTACTAAACtat > 1:5380/1‑140 (MQ=255) aaTAGCCGTATGCTGTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTatat < 2:8541/140‑1 (MQ=255) aGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATAtttt > 2:223804/1‑140 (MQ=255) cGTATGCTTTTCGGTTTATCAAGGCAAGAACTGGCACCTCAGGACTTTTCTACGACGGGTATTTGGCGCGTTGCGCGTGTTGTGGTTTTTGCTGCAGCAGCATTGTTACTTGTGGCAGCATTACTAGTCTATATTGTccc < 2:332023/140‑1 (MQ=255) tATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACCAATAAATATGGCGTTCCACATGTTACAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAg > 1:469794/1‑140 (MQ=255) tacTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGGCGAATAAATATGGCGTTCCACATGTTGCAATCTTTACTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGAt > 2:536462/3‑140 (MQ=255) ttttCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTACTTCATCAGCATTATTACTTGTGGCAACATTACTAAACTATATTTTCCCAGATGCg > 1:272338/1‑140 (MQ=255) ggTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTACACTATCTTTTCCCAGATGCGACaaa > 2:235731/1‑140 (MQ=255) ggTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATGGGACaaa > 2:459462/1‑140 (MQ=255) ggTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACaaa > 1:213464/1‑140 (MQ=255) gTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTATGACGAATTAATATTGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACaaaa < 1:55969/140‑1 (MQ=255) gTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACaaaa < 1:648486/140‑1 (MQ=255) tATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCCTTATCACCTGTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTa < 1:683323/140‑1 (MQ=255) tATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAGACTATATTTTCCCAGATGCGACAAAAGTa > 2:557928/1‑140 (MQ=255) aTCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTAt > 1:180772/1‑140 (MQ=255) aGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTATTTAc > 2:627734/1‑140 (MQ=255) gTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCCGCTGCGACAAAGGAAGTTACg > 1:334744/1‑140 (MQ=255) gTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCAACATGTTGCAATCGTTGCTTCATCAGCGTACTTACTTGTGGCAGCATTACTAAACTATATTTTACCAGATGCTACAAACGTCTTTagg > 2:269104/1‑138 (MQ=255) caacaaGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATCTTTTCCCAGATGACACAAAATAATTTACGtcagt > 2:340002/1‑136 (MQ=255) caacaaGCACCTCCAATCTTGTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATCACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTATTTACGTAtgt < 2:92998/140‑1 (MQ=255) caaGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTATTTACGTATGTGAc > 2:175300/1‑140 (MQ=255) aCCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTTCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTATTTACGTATGTGACTACCa > 1:23901/1‑140 (MQ=255) ccTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCCCATGTTGCAATCTTTGCTTCATCAGCATTATTACTTATGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTATTTACGTATGTGACTACCaa > 1:69449/1‑139 (MQ=255) cgccGCACTTTTATAAGAAGAACTGATCTGGCGTTCCGCAGTTCTCGACCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTATTTACGTATGTGACTACCAtc < 2:550637/138‑1 (MQ=255) ttttCTATGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTTCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTATTTACGTATGTGACTACCATCTCTACAgt < 1:560188/140‑1 (MQ=255) ttttCTATGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTATTTACGTATGTGACTACCATCTCTACAgt < 1:403373/140‑1 (MQ=255) tttCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGTTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTATTTACGTATGTGACTACCATCTCTACAgtg < 2:458629/140‑1 (MQ=255) tttCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTTTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTATTTACGTATGTGACTACCATCTCTACAgtg < 2:540836/140‑1 (MQ=255) tttCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTATTTACGTATGTGACTACCATCTCTACAgtg < 2:714958/140‑1 (MQ=255) ttCTAAGACGAATAAATATTGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATCGGACACAAGTATTTACGTATGTTAATACACACTCTACAgggt > 2:42162/1‑140 (MQ=255) ttCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTATTTACGTATGTGACTACCATCTCTACAgtgt < 1:40170/140‑1 (MQ=255) ttCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTATTTACGTATGTGACTACCATCTCTACAgtgt < 1:558228/140‑1 (MQ=255) | GGAATTCCGTTTGCTGCGGGCTTGATTAATTTTGTAGTATTAACCGCTGCTGCTTCATCATGTAACAGTGGTATATTCTCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCTAAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTTGTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTATTTACGTATGTGACTACCATCTCTACAGTGT > CP000730/1802489‑1802762 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |