Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,946,057 G→A 8.9% intergenic (+108/+366) USA300HOU_1807 → / ← epiG hypothetical protein/lantibiotic epidermin immunity protein F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,946,0570GA 8.9% 184.4 / 2.1 75intergenic (+108/+366)USA300HOU_1807/epiGhypothetical protein/lantibiotic epidermin immunity protein F
Reads supporting (aligned to +/- strand):  ref base G (41/26);  new base A (2/6);  total (43/32)
Fisher's exact test for biased strand distribution p-value = 6.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

ACGCTTCATCAACAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCGCGCATGTCGTCTTATTTTTACATTTATATAATAACACGC  >  CP000730/1945919‑1946193
                                                                                                                                          |                                                                                                                                        
aCGCTTCATCAACAACCTCACGATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGa                                                                                                                                         >  2:156762/1‑140 (MQ=255)
aCGCTTCATCAACAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCCATTAACGGa                                                                                                                                         >  2:35143/1‑140 (MQ=255)
aCGCTTCATCAACAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTAATTTTTATATCAATTAGCggc                                                                                                                                         >  2:359820/1‑139 (MQ=255)
  gCTTCATCAACAAAAACACAATTGATTAAACCTTAAACTCACATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCGTACAAACCAACCAGCCCGGTATATATTGAAACGTCATTTTTATTTTAATTAGCGGaaa                                                                                                                                       <  2:119048/140‑1 (MQ=255)
  cattCATAAACAAAATCACAATTGATTAAACCTTTAACTCAAATTTCCTTAATTCATACAACATTAATAACTAACCTTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGaaa                                                                                                                                       <  2:561301/138‑1 (MQ=255)
   attCATCAACAAAATCACAATTGATTAAACCTTAACCTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTGCTCACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGaaaa                                                                                                                                      <  2:98043/139‑1 (MQ=255)
     tcatcaACAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACTTAGCCCATAATATATTAAAATGTCATTTTTATTTCAATTAGCGGAAAATa                                                                                                                                    >  1:213425/1‑140 (MQ=255)
     tcatcaACAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACAACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATa                                                                                                                                    >  2:472261/1‑140 (MQ=255)
      catcaACAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATaa                                                                                                                                   >  2:206710/1‑140 (MQ=255)
      catcaACAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGAAAAATaa                                                                                                                                   >  2:707169/1‑140 (MQ=255)
       atcaACAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATaaa                                                                                                                                  >  2:378708/1‑140 (MQ=255)
             aagaTCACAATTGATTCAACCTTAAACCCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCAGCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAAcc                                                                                                                            <  2:486849/137‑1 (MQ=255)
              aaaTCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCa                                                                                                                           >  1:600256/1‑140 (MQ=255)
              aaaTCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCa                                                                                                                           <  1:273434/140‑1 (MQ=255)
                   aggattgtatacaCCTTAACAGCATATTTCCTCAATTTAGAAAATATGAATAAATGAAGCTTTCTTACATTCCACCGAGCTCATTATGTATTAATGCGTCTTTTTTATTTCAATTAAAGATAAATAAAAAAACCATTCCg                                                                                                                      <  2:406757/128‑1 (MQ=255)
                   acacTTAATTAAAACTAAAAATCAAATTTCCTTAATACCTAAAAAATTAATAACTAACCCTTTCTTACACACAACCTAGGCCATAATATATTAAAACGTCTTTTTTATTTCAATTAGAGAAAAAAAAAAAAACCATTCCg                                                                                                                      <  2:634708/140‑1 (MQ=255)
                   acaATTGGTTAAACCACAAACTCAAATTACCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAACCCACCTATCCCATAATATATTAAAACGTCATTTTTATTTCAATTAACTAAAAATAAAAAAACCATTCCg                                                                                                                      <  2:142234/140‑1 (MQ=255)
                    caATTGATTAAAACTTAACCTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTAAATTAGCGGAAAATAAAAAAACCATTACGc                                                                                                                     >  2:322328/1‑140 (MQ=255)
                     aaTTGAATAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCa                                                                                                                    <  1:281318/140‑1 (MQ=255)
                      aTTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCAt                                                                                                                   >  1:435488/1‑140 (MQ=255)
                      aTTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGAAAAATAAAAAAACCATTCAGCAt                                                                                                                   >  1:276864/1‑140 (MQ=255)
                          aTTAAACCTTAAACTCAAATTCCCCTCACCCATAAAACATTAATAACTAACCCCTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCTCATTAAc                                                                                                               <  2:577396/140‑1 (MQ=255)
                              aaccagaaaCTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCGAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAAAAAAAAAAAAAAAAACCATTCCGCATTAACTTTg                                                                                                           <  2:540760/134‑1 (MQ=255)
                              aaCCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTg                                                                                                           >  1:500328/1‑140 (MQ=255)
                                ccTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGc                                                                                                         >  2:168014/1‑140 (MQ=255)
                                  ttAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCaa                                                                                                       >  2:612659/1‑140 (MQ=255)
                                    aatcTCATATTTCCTTGATTACTAGAACATTAATAACTTACCCGTGCTTACTAACCACCTATCCCATATTAGATGAAAACGTTATTTTTTTTTCAATTAGAAAAAAAAAAAAAAACCATTCCGCATTAACTTTGGCAAGt                                                                                                     <  2:160308/137‑1 (MQ=255)
                                       cTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTga                                                                                                  >  1:717602/1‑139 (MQ=255)
                                         cAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGaa                                                                                                >  1:479266/1‑140 (MQ=255)
                                         cAAATTTCCTTAATTCATAAAAAATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGaa                                                                                                >  2:424646/1‑140 (MQ=255)
                                            aTTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATgg                                                                                             <  1:472261/140‑1 (MQ=255)
                                            aTTTCCTTAATTCATAAAACAATAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATgg                                                                                             <  1:113984/140‑1 (MQ=255)
                                             tttCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGt                                                                                            >  1:243638/1‑140 (MQ=255)
                                             tttCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGAATTAACTTTGGCAAGTTGGAATGGt                                                                                            >  1:59617/1‑140 (MQ=255)
                                              ttCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAACATAAAACAACCCTTCCGCATTAACTTTGGCAAGTTGGAATGAtt                                                                                           >  1:312009/1‑140 (MQ=255)
                                              ttCCTTAATTCATAAAACATTAATAAATAACCCTTTCTTACAAACCACCTAGACCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTACCTTTGGCAAGTTGAAAAGGtt                                                                                           >  2:598049/1‑140 (MQ=255)
                                                 catAATTCAAAAAACATTAATAACTAGCCCTGTCATAAAAACCACCTAAACCATAATATATTAAAACGTCATTTTTATTTCAATTATCAGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTaaa                                                                                        <  2:444738/138‑1 (MQ=255)
                                                           aaaaCATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATAtttt                                                                              >  2:317774/1‑140 (MQ=255)
                                                           aaaaCATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATAtttt                                                                              >  1:213426/1‑140 (MQ=255)
                                                           aaaaCATTAATAACAAACCCATTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATACAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATAtttt                                                                              <  1:487286/140‑1 (MQ=255)
                                                              aCATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGCATGGTTAAAAAAATATTTTAGa                                                                           >  1:670790/1‑140 (MQ=255)
                                                              aCATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTaaa                                                                           >  1:370199/1‑138 (MQ=255)
                                                              aCATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACAATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGa                                                                           >  1:37087/1‑140 (MQ=255)
                                                                aTTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAg                                                                         <  1:546682/140‑1 (MQ=255)
                                                                  taataaCTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCTGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGTTTAAAACAATATTTTTAAAGct                                                                       >  2:132578/1‑139 (MQ=255)
                                                                   tataaTTAACCTTTTCTTACAAACCAACTAGCCCATAATAAATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCa                                                                      <  1:35143/139‑1 (MQ=255)
                                                                   aataaCTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCa                                                                      >  1:468134/1‑140 (MQ=255)
                                                                     taactaacCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGc                                                                    >  2:674331/1‑140 (MQ=255)
                                                                     taactaacCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTAAATTAGCGGGAAATAAACAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAACAAATTTTAGaacaagac                                                                    >  2:212144/1‑134 (MQ=255)
                                                                     taactaacCCTTTCTTACAAACCACATAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAACTATTTTAGAAGCCAga                                                                    >  2:614460/1‑139 (MQ=255)
                                                                       actaacCCTTTCTTACAAACCACCTAGCCCATAATATATTAATACGTCATTTTTATTTCAATTAGAGAAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGt                                                                  <  2:233708/140‑1 (MQ=255)
                                                                        ctaacCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCCAGTCGGAATGGTTAAAAAACTATTTTAGAAGCCAGCGtt                                                                 >  2:341880/1‑140 (MQ=255)
                                                                           acCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGCTGGCATGATTAAAGAAATATTTTAGAAGCCAGCGTTTgg                                                              >  2:384199/1‑140 (MQ=255)
                                                                            cccTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCAAACGTTTgga                                                             >  1:14874/1‑139 (MQ=255)
                                                                                ttCTTACAAACCACCTAGCCCATAATATATTAAATCGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGgcttgc                                                         <  1:177016/140‑1 (MQ=255)
                                                                                    tACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAACTATTTTAGAAGCCAGCGTTTGGCTTGCTTat                                                     >  2:545699/1‑139 (MQ=255)
                                                                                         accaccTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAgacgac                                                >  1:484068/1‑140 (MQ=255)
                                                                                                  cccATAATATAGTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTcgcg                                       <  2:183203/140‑1 (MQ=255)
                                                                                                   ccATAATCTATTAAATCGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCgcgc                                      <  2:502950/140‑1 (MQ=255)
                                                                                                   ccATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCgcgc                                      <  1:424646/140‑1 (MQ=255)
                                                                                                   ccATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCgcgc                                      <  2:479266/140‑1 (MQ=255)
                                                                                                   ccATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATGTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCgcgc                                      <  1:209590/140‑1 (MQ=255)
                                                                                                    cATAATGTATTAATACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCGCGCa                                     <  2:213744/140‑1 (MQ=255)
                                                                                                                  aCGTCATTTTTATTTCAATTAGCGGAACATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCGTGCATGTCGTCTTAtttt                       >  2:549308/1‑140 (MQ=255)
                                                                                                                  aCGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCGCGCATGTCGTCTTAtttt                       >  2:395562/1‑140 (MQ=255)
                                                                                                                  aCGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCGCGCATGTCGTCTTAtttt                       >  2:656692/1‑140 (MQ=255)
                                                                                                                   cGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCGCGCATGTCGTCTTAttttt                      <  1:156762/140‑1 (MQ=255)
                                                                                                                     tCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCGCGCATGTCGTCTTATTTTTAc                    <  2:312009/140‑1 (MQ=255)
                                                                                                                      cATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAAATTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCGAGCATGTCGTCTTATTTTTACa                   >  1:549132/1‑140 (MQ=255)
                                                                                                                       aTTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCGCGCATGTCGTCTTATTTTTACAt                  >  1:301752/1‑140 (MQ=255)
                                                                                                                       aTTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCGCGCATGTCGTCTTATTTTTACAt                  >  1:143109/1‑140 (MQ=255)
                                                                                                                        tttttATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCGCGCATGTCGTCTTATTTTTACAtt                 >  1:726305/1‑140 (MQ=255)
                                                                                                                            tATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCGCGCATGTCGTCTTATTTTTACATTtata             <  1:359820/140‑1 (MQ=255)
                                                                                                                             aTTTTAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCGCGCATGTCGTCTTATTTTTACATTtatat            <  1:322328/140‑1 (MQ=255)
                                                                                                                                tCAATTAGCGGAAAATAAAAAAACCATTCCTCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCGCGCATGTCGTCTTATTTTTACATTTATataat         <  2:250354/140‑1 (MQ=255)
                                                                                                                                       gCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCGCGCATGTCGTCTTATTTTTACATTTATATAATAACACGc  <  1:612659/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                        
ACGCTTCATCAACAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATAATATATTAAAACGTCATTTTTATTTCAATTAGCGGAAAATAAAAAAACCATTCCGCATTAACTTTGGCAAGTTGGAATGGTTAAAAAAATATTTTAGAAGCCAGCGTTTGGCTTGCTTAGACGACATGTTCGCGCATGTCGTCTTATTTTTACATTTATATAATAACACGC  >  CP000730/1945919‑1946193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: