Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 111,512 A→G 32.1% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730111,5120AG32.1% 87.3 / 63.3 84S194S (TCA→TCGUSA300HOU_0110possible staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (27/30);  new base G (11/16);  total (38/46)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

TACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAA  >  CP000730/111374‑111649
                                                                                                                                          |                                                                                                                                         
tACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATCTAGCAATTTTAAAGATTTAATGAATTCTATAGATGGAGTTATATCATATAATCCAGAGGTGCCGAGTTATTTAg                                                                                                                                          >  2:32055/1‑140 (MQ=25)
tACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGAGGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAg                                                                                                                                          <  2:472869/140‑1 (MQ=255)
  cTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCt                                                                                                                                        <  2:437373/140‑1 (MQ=255)
   tGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCta                                                                                                                                       <  1:727685/140‑2 (MQ=255)
   tGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCta                                                                                                                                       >  1:360067/1‑139 (MQ=255)
   tGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTc                                                                                                                                       <  2:218750/140‑1 (MQ=255)
   tGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTc                                                                                                                                       <  2:427190/140‑1 (MQ=255)
      agAAGTTAAAAAAAAAAACATAAAAAAAAAAATCGAAAACTATAAGTTTTTTGCGCAATATAGCAATTTTAAAGAATTAAGGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                    <  1:64018/139‑1 (MQ=17)
       gAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAata                                                                                                                                   <  2:611753/140‑1 (MQ=255)
        aaGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTTTTTTTATATCAATATTAAAGATTTAATGAATTATAAAGATGGAGATATATAATATAATCAAGAGATGCCGAGTTATTCGGCtagatgt                                                                                                                                  >  2:3408/1‑134 (MQ=18)
        aaGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAatat                                                                                                                                  >  1:393678/1‑140 (MQ=255)
          gTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGACGTGCCGAGTTATTCGGCTAAATATCa                                                                                                                                <  1:646586/140‑1 (MQ=255)
           ttAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTAAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCAGAGTTATTAAGCTCAATATCac                                                                                                                               >  2:475107/1‑139 (MQ=255)
               agaagaAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                                                           <  1:30120/140‑1 (MQ=255)
               agaaaaAAAAAGAAAAAAAGAAATCGAAAAGTTTAAGTTTTTTGCGGAAGAGAACAAGTTCAAAGAGTTAATTAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTGATTCGGCTAAATATCAATTaa                                                                                                                           <  2:211249/140‑1 (MQ=17)
                gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                                                          >  2:69643/1‑140 (MQ=255)
                gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAACTTTTTGCGCAATACAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATACCATATAATCCAGAGGTCCCGAGTTATTCAGCTCAATATCAATTAAc                                                                                                                          >  2:589343/1‑140 (MQ=21)
                  agaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTa                                                                                                                        <  1:261914/140‑1 (MQ=255)
                  agaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTa                                                                                                                        <  1:3237/140‑1 (MQ=255)
                  agaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTa                                                                                                                        >  2:190929/1‑140 (MQ=255)
                   gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCAAAATATAAATTAACTaa                                                                                                                       >  2:26519/1‑140 (MQ=255)
                   gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATAATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTaa                                                                                                                       >  1:328228/1‑140 (MQ=255)
                      aaCATAAAAAAAGAAATCGAAAACATTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATCATACCGAGGTGCCGAGTTATACGGCTAAATATCAATTAACTAatga                                                                                                                    >  2:151286/1‑140 (MQ=18)
                       aaaTAAAAAAATAAATCGATAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgat                                                                                                                   <  1:157450/138‑1 (MQ=255)
                       aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATCGACATGTTTAAGATGTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTCTTCGGCTAAATATCAATTAACTAatgat                                                                                                                   >  2:697947/1‑140 (MQ=14)
                       aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                                                                   >  1:637115/1‑140 (MQ=255)
                       aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgat                                                                                                                   >  2:378464/1‑140 (MQ=255)
                       aCATAAAAAAAGAAATCGAAAACTGTAAGTTTTTTGCGCAATATAGAAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATGATATAATACAGGTGTAGCGAGTTATTCGGCTAAATATCAATTAACTAatggt                                                                                                                   >  2:329573/1‑138 (MQ=17)
                         aTAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                                                                 <  2:656636/140‑1 (MQ=255)
                          tAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATTAATTAACTAATgatgat                                                                                                                >  1:154000/1‑140 (MQ=255)
                           aaaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAtt                                                                                                               <  2:674695/140‑1 (MQ=255)
                             aaaaaGAAATCTAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                             <  1:686695/140‑1 (MQ=255)
                             aaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATGATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                             <  1:154713/140‑1 (MQ=255)
                                aataaagccaaaaCTTTAATTTTTTTTCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAAt                                                                                                          <  1:161266/131‑1 (MQ=17)
                                  gAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGAAATCTTAAAGATTTAATGAATTATAATGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGt                                                                                                        <  1:491478/140‑1 (MQ=255)
                                   aaaTCGAAAACTTTAAGTTTTTTACGCAATATAGCAATTTTAAAGATTTAATGAATTAGAAAGATGGAGATATATCATATAATCCATAGGTGCCTAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTa                                                                                                       <  2:41700/140‑1 (MQ=255)
                                    aaTCGAACACTTTAAGTTTTTTGGGGAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                      <  2:507742/140‑1 (MQ=255)
                                    aaTCGAAAACTTTAAGTTTTTTGCTCAATATAGCAATTTTAAAGATTTAATAAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGACGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                      >  2:587002/1‑140 (MQ=255)
                                    aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAAGATGATTATAATGTaa                                                                                                      >  1:153753/1‑140 (MQ=255)
                                    aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                      <  2:676522/140‑1 (MQ=255)
                                    aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGAAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                      <  2:372350/140‑1 (MQ=255)
                                    aaTCAAAAACTTTAGTTTTGATGAGAAATATAGCAATTTTAAAGATTTAATGAATGATAAAGATGGAGATATATCATATAATCCAGAGGTGCGGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                      <  2:658367/140‑1 (MQ=18)
                                     aTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGGTTATAATGTaaa                                                                                                     >  2:247139/1‑140 (MQ=255)
                                     aTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaa                                                                                                     >  1:706045/1‑140 (MQ=255)
                                      tCGAGAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGTGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                    <  2:529003/140‑1 (MQ=18)
                                      tCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                    <  2:698098/140‑1 (MQ=255)
                                      tCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCACAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                    >  1:335543/1‑140 (MQ=255)
                                      tCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATAATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                    >  2:159456/1‑140 (MQ=255)
                                          aaaCTTTAAGTTTTTTGCGCAATATAGTAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                                                <  1:276314/140‑1 (MQ=255)
                                          aaaCTTTAAGTTTTTTGCGAAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                                                <  1:614723/140‑1 (MQ=255)
                                             cTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                                                             <  1:98595/140‑1 (MQ=255)
                                                 aaGTTTTTTGCTCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                         <  1:190929/140‑1 (MQ=255)
                                                 aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                         >  2:398837/1‑140 (MQ=255)
                                                 aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                         <  1:97154/140‑1 (MQ=255)
                                                  aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaaa                                                                                        >  1:666693/1‑140 (MQ=255)
                                                          gcgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGATATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGAAAAACAATTACGTAAAAGatataa                                                                                >  1:487511/1‑138 (MQ=255)
                                                          gcgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATAGGa                                                                                >  1:610777/1‑140 (MQ=255)
                                                               atatAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAc                                                                           >  2:397488/1‑140 (MQ=255)
                                                                tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATAcc                                                                          >  2:599751/1‑140 (MQ=255)
                                                                  tcgCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATAGAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccga                                                                        <  2:389283/138‑3 (MQ=11)
                                                                         tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATAATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATaa                                                                 >  1:550683/1‑140 (MQ=255)
                                                                         ttaaacgATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATaa                                                                 <  2:384589/134‑1 (MQ=255)
                                                                               gATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                           <  2:538574/140‑1 (MQ=255)
                                                                               gATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTCATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGAGcc                                                           >  1:470542/1‑140 (MQ=255)
                                                                                     aTGAATTATAAAGATGGAGATATCTTGACGAAGCCATAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGAAATGAAATACCAACAAATAAAGCGACGAAGCt                                                     >  1:172920/1‑140 (MQ=17)
                                                                                      tGAATTATAAAGATTGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtg                                                    <  2:513770/140‑1 (MQ=255)
                                                                                         aTTATAAAGATGGATACTTACCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTACAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttg                                                 <  1:636107/140‑1 (MQ=14)
                                                                                           tataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTgttgtt                                               <  2:505298/140‑1 (MQ=255)
                                                                                                       gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAgg                                   <  1:378464/140‑1 (MQ=255)
                                                                                                         tatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGCTGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCTGCGAAGATGTTGTTGAAAGGTACAGGGa                                 >  2:590323/1‑140 (MQ=255)
                                                                                                             tCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTGGAAAGGTACAGGGAAttt                             >  2:238481/1‑140 (MQ=255)
                                                                                                                    aTCCAGAGGTGCCAAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                      <  1:449343/140‑1 (MQ=255)
                                                                                                                      cctgagGTGCCGAGTTATTCAGCTCAATAACCATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATTACATACCAACAAAGAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                    <  1:115183/137‑1 (MQ=17)
                                                                                                                      ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                    <  2:704754/140‑1 (MQ=255)
                                                                                                                       cAGATGTGACGAGTTATTCAGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAACGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                   <  1:246021/140‑1 (MQ=255)
                                                                                                                       cAGAGGTGCCGAGTTATACATCTCAAGATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                   <  1:14088/140‑1 (MQ=255)
                                                                                                                         gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGCTTATAATGTAAAACAATTACGTAAAAGATTTGCCATACCAACAACTAAAGCGCCGAAGATGTTCTTGAAAGGTACAGGCAATTAAAAAGAttactc                 >  1:596776/1‑136 (MQ=14)
                                                                                                                           ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAGTATAATGTAAAACAATTACGTGAAAGATATGACATACCAACAACTAAAACGCCGAAGCTGTTGTTGAAAGGTACAGCGAATTTAAAAAGTTCATCAg               >  1:230037/1‑140 (MQ=17)
                                                                                                                                  aGTTATTCAGCTCAATATCAATTAACTAATTATGATTATAATGTAAAACAATTATGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGTTTCATCAGTTGGata        <  2:271393/140‑1 (MQ=255)
                                                                                                                                     tataCAGCTCAAAAAGAATGGACTAAGGATGATTATAAGGTAAATCAGGAACGGAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaa     >  2:719225/1‑140 (MQ=14)
                                                                                                                                      aTTCAGCTCAATGTCAATTAACAAATGATAATTATAATGTAAAACAATTACGTAAAAGACATGACATAGCTACTAATAAAGCGCCGAAGCTGTTGTTGAAAAGTAGAGGGAATTTAAAAGGTTCATCAGTTGGATATaaa    <  2:459651/140‑1 (MQ=17)
                                                                                                                                      aTTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaa    <  1:575460/140‑1 (MQ=255)
                                                                                                                                        tCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaa  >  2:348057/1‑140 (MQ=255)
                                                                                                                                        tCAGCTCAATATCAATTAACTAATAATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaa  >  1:464136/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                         
TACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAA  >  CP000730/111374‑111649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: