Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,279,7050GA99.1% 203.4 / ‑6.0 72intergenic (+1373/+6659)mtlD/glmMmannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (36/34);  minor base C (1/0);  total (38/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT  >  CP000730/2279573‑2279841
                                                                                                                                    |                                                                                                                                        
ccGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCCGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTATCTTACGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgttgt                                                                                                                                   >  1:137667/1‑140 (MQ=255)
 cGTTATTAGTTTGTTCTACTTCTTGTTGACTAGTTACATCGTTATCCATTGTCGGACTGTTTTTTGATGCATTTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTgttgtt                                                                                                                                  <  1:14856/140‑1 (MQ=255)
 cGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCATTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGAGGTTTATAATTTGAATCATCAGGTGCTGATGTTAATgttgtt                                                                                                                                  >  1:320555/1‑140 (MQ=255)
  gTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGttt                                                                                                                                 <  2:218445/140‑1 (MQ=255)
        aggtGATTTTACTTCTGGTTTACCACTTACATCGTTTTCCATTGTCGGACCGTTTTTTGCTGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTg                                                                                                                           <  2:70199/137‑1 (MQ=255)
         gTTGATTCTACTTCTGTTTTATTAGTTACTGCGTTATCCATTCTTGGACCTTGGTTTGATGCATCTCCACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGt                                                                                                                          <  1:525481/140‑1 (MQ=255)
         gTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTTCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTATTTCAcaggt                                                                                                                          >  1:286792/1‑136 (MQ=255)
             aTTCTACTTCTGGTTTACTAGTTACATCGTTATCTATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGcc                                                                                                                      <  1:557756/140‑1 (MQ=255)
                 tagttCTGGTTTACTAGTTACATCGTTATACATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTAGCATTTGCATCATCAGTTGCTGTTGTTACTGTTGGTGAACCTGTTGCCGCAt                                                                                                                  >  2:489663/4‑140 (MQ=255)
                  aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATc                                                                                                                 >  1:87937/1‑140 (MQ=255)
                  aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTAGCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATc                                                                                                                 >  2:398755/1‑140 (MQ=255)
                   cTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGAGGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCCTCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCAGCATCa                                                                                                                >  1:238351/1‑140 (MQ=255)
                    ttgtGGTTTCCTCGTTACTTCGTTATCCATTGTCGGACTGTTTGTTGCTGCATCTACACTAGATTTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCAc                                                                                                               <  1:177692/137‑1 (MQ=255)
                    ttCTGGTTTTCTAGTTACATCGTTATTCATTGTCGGACTGTTTGTTGATGCTTCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCAc                                                                                                               <  1:284753/140‑1 (MQ=255)
                    ttCTGGTTTACTAGTTACGTCGTTTTCCATTGTCTGACTGTTTGTTCATGCATCTACACTAGAATTGTTATTAGCTTGCGTTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCAc                                                                                                               <  2:112897/140‑1 (MQ=255)
                    ttCTGGTTTACTAGTTACATCTTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCAc                                                                                                               <  2:494459/140‑1 (MQ=255)
                    ttCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTTTTTTTTGATTCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCAc                                                                                                               <  1:12505/140‑1 (MQ=255)
                    ttCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAGCTGTTGCCGCATCAc                                                                                                               <  1:541040/140‑1 (MQ=255)
                      cTGGTTTACTAGTTACATCGTTATCCATTGTCGGACCGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACGGTCGTGTCTCCTGTTTCCGCATCACta                                                                                                             >  1:502987/1‑140 (MQ=255)
                       tGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtat                                                                                                            >  1:436476/1‑140 (MQ=255)
                         gTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTCCACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtatta                                                                                                          >  1:428401/1‑140 (MQ=255)
                         gTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtatta                                                                                                          >  1:171970/1‑140 (MQ=255)
                            tACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTAttt                                                                                                       >  2:636278/1‑140 (MQ=255)
                            tACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTAttt                                                                                                       >  1:641249/1‑140 (MQ=255)
                             aCTAGTTAAATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGGATTATAAATACCCTGCGGTTTACCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTg                                                                                                      <  1:346166/140‑1 (MQ=255)
                              cTAGTTACATCGTTATCCATTGTCGGATTGTTTGTTGATGCATCTACACTAGAATTTTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTgg                                                                                                     <  1:350771/140‑1 (MQ=255)
                                aGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATTTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgtg                                                                                                   <  1:235631/140‑1 (MQ=255)
                                          ttATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgaga                                                                                         >  2:263845/1‑140 (MQ=255)
                                           tATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTTTCGGAGaa                                                                                        >  2:358534/1‑140 (MQ=255)
                                           tATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGaa                                                                                        >  1:561655/1‑140 (MQ=255)
                                           tATCCATTGTCGGACTGTTTGTTGATGCATCTACAATAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCCCCTGTTGCCGGAGCACTAGTATTTGGTGTTTTCGGAGaa                                                                                        >  2:328637/1‑140 (MQ=255)
                                             tCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGc                                                                                      <  1:79580/140‑1 (MQ=255)
                                              ccATCGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCg                                                                                     <  2:527866/140‑1 (MQ=255)
                                                aTTGTCGGACTGTTTGCTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTc                                                                                   <  1:629041/140‑1 (MQ=255)
                                                  tGTCAGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCCCCTGTTGCCGCATGACTATTATTTGGTGTTGTCGGAGAAGCGTCTg                                                                                 >  1:493832/1‑140 (MQ=255)
                                                    tCGGTCTGTTTGTTGGTGGTTGTACACTAGTATTGTTTTTAGTTTGCGGTTTATCATTTGCATCTTCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCTGTATCACTATTATTTGGAGTTGTCGgacgggagtcttat                                                                               <  2:56957/140‑13 (MQ=255)
                                                    tCGGCCTGTTTGTTGATGCATCTGCACTATAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCt                                                                               <  2:354768/140‑1 (MQ=255)
                                                    tCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCt                                                                               >  1:187275/1‑140 (MQ=255)
                                                         ctttttggtGATGCATTTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAATCGTCTGCTTTGcc                                                                          <  1:143261/137‑1 (MQ=255)
                                                         cTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTGTCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTCTTATTTGATGTTGTCGGAGAAGCGGCTGCTTTTcc                                                                          >  2:641357/1‑140 (MQ=255)
                                                          tgtgtgttGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCTGAGAAGCGTCTGTTTTGCCa                                                                         <  1:459169/140‑1 (MQ=255)
                                                           gtttgttGATGCATCTACACTAGAATGGTTAGTAGCTTGCGGTTGATCAGTTGCAGCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTGGTCGGAGAAGCGTCTGCTTTGCCag                                                                        >  2:375113/1‑139 (MQ=255)
                                                                ttgATGCATCTACACTGGAATTGTTATTAGCTTGCGGTTTTTCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCt                                                                   <  2:340959/140‑1 (MQ=255)
                                                                ttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCt                                                                   >  1:393444/1‑140 (MQ=255)
                                                                 tgATGCATCTACACTAGAATTGTTATTAACTTGCGGTTTATCATTTGCATCATCCGTTGCTGATGTTACTGTTGTTTCAGCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTg                                                                  >  1:469140/1‑140 (MQ=255)
                                                                  gATGTATCTACACTAGAATTGTTATTAGCTTGCGGTTTTTCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgt                                                                 <  2:591243/140‑1 (MQ=255)
                                                                     gCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTCCTGTTGTTTCACCTGTCGCCGCATCACTCTTATTTGGTGTTGTCGGAGAAGCATCTGCTATGCCATTAGCcgtcgt                                                              >  1:47919/1‑140 (MQ=255)
                                                                          tACACTAGAATTGTTATTATCTTTCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAg                                                         <  2:655504/140‑1 (MQ=255)
                                                                          tACACTAGAATTGTTATTAACTTGCGGTGTATCATTTGCATCATCAGTTGCTGAGGTTACTGTTGTTTCACCTGTTGCCGCATCAGTATTATTTGGTGTTGTCGGAGCAGCGTCTGCTTTGCCAGTAGCTGCAGTCTCAg                                                         >  2:482154/1‑140 (MQ=255)
                                                                                  aaTTGTTATTTGCTTGCTGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTa                                                 <  1:154562/140‑1 (MQ=255)
                                                                                    ttGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAgg                                               >  1:265945/1‑140 (MQ=255)
                                                                                      gTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCTATTAGCTGTCGTCTCAGATACGTTAGGtt                                             >  1:274555/1‑140 (MQ=255)
                                                                                         attaGCTTGTGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTc                                          >  1:66362/1‑140 (MQ=255)
                                                                                         attaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTTTTGTCGGAGAAACGTCTGCTTTGCCATTAGCTGTCGTCTAAGATACGTTAGGTTGTc                                          >  1:36343/1‑140 (MQ=255)
                                                                                                    gTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCt                               >  2:671301/1‑140 (MQ=255)
                                                                                                    gTTTATCATTTGCATCATCAGTTGCTCATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCt                               >  2:437170/1‑140 (MQ=255)
                                                                                                     tttCTCATTTGCATCATCATTTGCTTATGTTACTGTTGTTTCTCCTGTTGCCGCATCACTATTATTTGGTGTTGTGGGAGACGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTg                              <  2:38669/140‑1 (MQ=255)
                                                                                                      ttATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTgg                             >  2:667362/1‑140 (MQ=255)
                                                                                                      ttATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTgg                             <  1:364880/140‑1 (MQ=255)
                                                                                                       tATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgt                            <  1:99871/140‑1 (MQ=255)
                                                                                                       tATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgt                            <  1:434647/140‑1 (MQ=255)
                                                                                                         tCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgtgt                          <  1:638000/140‑1 (MQ=255)
                                                                                                         tCAATTGCATCATCAGTTGCTGATGTTACTGTTGTTGCCCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCACATACGTCAATGTGGCCAGTATTTTCTGgtgt                          <  1:720501/140‑1 (MQ=255)
                                                                                                            tttGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCATATACGTTAGGTTGTCCAGCATTTTTTGGTGTTGc                       >  1:348650/1‑140 (MQ=255)
                                                                                                                  tcatcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCCTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGc                 >  2:279886/1‑140 (MQ=255)
                                                                                                                   catcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCa                >  1:564358/1‑140 (MQ=255)
                                                                                                                        gTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGa           >  2:268230/1‑140 (MQ=255)
                                                                                                                               aTGTTACTGTTGTTTCCCCTGTTGCCGCATCACTATTATTTGGTGTTGGCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCt    <  2:171970/140‑1 (MQ=255)
                                                                                                                               aTGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCt    <  2:428401/140‑1 (MQ=255)
                                                                                                                               aTGTTACTGTTGTTTCACCTGTTGCCGCATCACTATAATCTGATGTTGTCGGAGAAGCGCCTGCTTTGCCCTTAGCTGTCGTCTCAGTTACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCGTTTGAATTTgat    >  2:605864/1‑138 (MQ=255)
                                                                                                                                 gTTACTGTTGTTTCCCCTGTTGCCGCATCACAATGATTGGGTGTTGTCGGAGAAGCGTATGGTTTGCCATAAGCTGTAGACTCGGATTAGTTAAGTTGTCCAGTATTTACTGGTGTTGAGTTAGCATTTGAATTTACTGt  >  2:20656/1‑140 (MQ=255)
                                                                                                                                 gTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGt  <  2:555852/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                        
CCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT  >  CP000730/2279573‑2279841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: