Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,289,325 C→A 100% Q55H (CAG→CAT USA300HOU_2153 ← hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,289,3250CA100.0% 175.6 / NA 64Q55H (CAG→CATUSA300HOU_2153hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (36/28);  total (36/28)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACAATTTTTAACGTACTGA  >  CP000730/2289191‑2289461
                                                                                                                                      |                                                                                                                                        
cTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGAcc                                                                                                                                     <  1:665271/140‑1 (MQ=255)
    ctctACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTCTCTGATCACCAATa                                                                                                                                 >  2:503304/1‑140 (MQ=255)
      ctACCAAGTTGTTCTAACGCACGTCTAATTTATGGTTGGAATATTACTATTAAAGAAAAGACACACAATTGAATAACGATATCGAATAATTTAAATGTTGAAGTCAAGTTCAATATCATACTTATCTGATGACAAATaaa                                                                                                                               >  1:137638/1‑139 (MQ=255)
            aGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATACCAATTAc                                                                                                                         >  2:584517/1‑140 (MQ=255)
            aGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTAc                                                                                                                         >  2:45805/1‑140 (MQ=255)
              ttgttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTa                                                                                                                       >  2:625493/1‑140 (MQ=255)
               ttttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTaa                                                                                                                      <  1:594095/138‑1 (MQ=255)
               tgttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTaa                                                                                                                      <  1:594652/140‑1 (MQ=255)
               tgttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTaa                                                                                                                      <  2:217056/140‑1 (MQ=255)
               tgttCGAACGCACGTCTACTTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAACTTCAATATCATACTTATCTGATGACCAATAACAATTACTaa                                                                                                                      <  2:556073/140‑1 (MQ=255)
                 ttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAata                                                                                                                    >  2:403662/1‑140 (MQ=255)
                   cTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGGCAAGTTCAATATCATACTTATCCGATGACCAATAACAATTACTAATATc                                                                                                                  >  2:101285/1‑140 (MQ=255)
                     aaCGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATccc                                                                                                                <  1:663104/140‑1 (MQ=255)
                       cGCACGTCTAATTCCTGGTTGGAATATTACTATTATAGCTAATACCCCCCGTTGTATAGCGATATCGAATAATTTTGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCTATAACAATTTCTAATATCCCtt                                                                                                              <  2:269449/140‑1 (MQ=255)
                              cTAATTTCTGGTTGGCATATTACTATTAAAGCTAATAACTCCCAATGAATAACGCTATCTAATAATTTAGATTTTGCAGTCAAGTTCAATTACCTACTTATTTGATTGCCAATAACAATTGCTAAAGTCCCTTTCAGTaa                                                                                                       >  2:317034/1‑140 (MQ=255)
                              cTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACATTTTTTAATATCCCtttttatat                                                                                                       >  2:683696/1‑134 (MQ=255)
                               tAATTTCTGGTTGGAATATTACTATTAACGCTAATTCCCCCTATTGATTAACGATATCGAATAGTATTGTAGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAAt                                                                                                      <  1:716107/140‑1 (MQ=255)
                               tAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAAAGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGAACAATAACAATTACTAATATACCTTTAAGAAAt                                                                                                      >  1:254882/1‑140 (MQ=255)
                                  tttCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCAGTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGt                                                                                                   <  2:667039/140‑1 (MQ=255)
                                       ggTTGGAATATTACTATTAAATCTAATCCCCTCCATTGAATAAGGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATGTGAAGACCAATAACAATTACTAATTTCCCTTTTTGTAATTGTATCGc                                                                                              <  2:358702/140‑1 (MQ=255)
                                       ggTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGc                                                                                              <  1:45805/140‑1 (MQ=255)
                                          tGGAAAATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCttt                                                                                           <  2:274264/140‑1 (MQ=255)
                                              atatTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGtt                                                                                       >  1:131540/1‑140 (MQ=255)
                                              atatTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGtt                                                                                       >  1:405989/1‑140 (MQ=255)
                                                 ttACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTccc                                                                                    <  1:625493/140‑1 (MQ=255)
                                                  tACCATTAAACATAATACCCCCCAATAAATAACCATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAACAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCt                                                                                   <  2:288821/140‑1 (MQ=255)
                                                   aCTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCtt                                                                                  >  1:311454/1‑140 (MQ=255)
                                                   aCTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCtt                                                                                  >  1:82891/1‑140 (MQ=255)
                                                        tAAAGCTAATACCCCCCATTGAATAACGATATCGAATTATTTAGATGTTGCAGTCAAGTTCAATATCATTCTTATTTGATGATTAATTACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGa                                                                             <  1:69094/140‑1 (MQ=255)
                                                         acaGCGAATGCCCCCCATTGAATAACGAGATCGAATAATTTAGATGTTGCAGTCAAGTTCAACTTCATACTAATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCGCTTAAAGAc                                                                            <  2:526592/138‑1 (MQ=255)
                                                          aaGCTAATACCCCCCATTGAATAACTATATCGAATAATTTACATGTTGCAGTCAAGATCAATAACAAACTTAACAGATGACCAATAACAATTACTAAAATCCCTTAAAGAAAATGTATCGCTTTAGTTCCCTTAAAGAcc                                                                           >  1:30816/1‑140 (MQ=255)
                                                           aGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCg                                                                          <  1:627557/140‑1 (MQ=255)
                                                               aaTACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACAAATAACAATTACTAATATCCCTTTAAGTAATTGTATAGCTTTATTTCCCTTAAAGACCGtgat                                                                      >  1:8849/1‑140 (MQ=255)
                                                                aTACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGtgatg                                                                     >  2:141980/1‑140 (MQ=255)
                                                                aTACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGtgatg                                                                     >  2:142255/1‑140 (MQ=255)
                                                                aTACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGtgatg                                                                     >  1:702566/1‑140 (MQ=255)
                                                                   ggccccATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCTCTTAAAGACCGTGATgaga                                                                  >  2:188065/3‑140 (MQ=255)
                                                                   ccccccATTGAATAACGGTATCGAATAATTTAGATGTTACAGTGAAGTTCAATATCATACTTATCTGATGACCAATAACATTTACTAATATCCCTTTGAGTAATTGTAGAGTTTTACATCCTTTAAAAACCGtcttgcga                                                                  >  2:160580/1‑133 (MQ=255)
                                                                   ccccccATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATgaga                                                                  >  1:727144/1‑140 (MQ=255)
                                                                   ccccccATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATgaga                                                                  >  2:258636/1‑140 (MQ=255)
                                                                   ccccccATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATAGCTTTAGTTCCCTTAAAGACCGTGATgaga                                                                  >  1:341896/1‑140 (MQ=255)
                                                                     ccccATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCATGATGagaag                                                                >  2:344489/1‑140 (MQ=255)
                                                                     ccccATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACGTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGagaag                                                                >  1:444570/1‑140 (MQ=255)
                                                                         aTTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCGATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATaa                                                            >  2:513164/1‑140 (MQ=255)
                                                                          ttGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATaaa                                                           >  1:265686/1‑140 (MQ=255)
                                                                              aTAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCTTTAAAAACCGTGATGAGCAGATAAAGTAc                                                       >  2:632432/1‑140 (MQ=255)
                                                                                 aCGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCTCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATa                                                    <  1:241860/140‑1 (MQ=255)
                                                                                   gATATCGAATAATTTAAATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATAcc                                                  <  1:600973/140‑1 (MQ=255)
                                                                                     tatCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCACTTAAAGACCGTGATGAGAAGATAAAGTACATACCaa                                                <  2:35445/140‑1 (MQ=255)
                                                                                      atCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACAAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCaaa                                               <  2:109878/140‑1 (MQ=255)
                                                                                       tCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTAACGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAAc                                              >  1:588389/1‑140 (MQ=255)
                                                                                         gAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTa                                            >  1:68313/1‑140 (MQ=255)
                                                                                                  aGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAAt                                   >  2:625148/1‑140 (MQ=255)
                                                                                                   gATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTAATGAGAAGATAAAGTACATACCAAACTATCAGTAAATc                                  >  1:67329/1‑140 (MQ=255)
                                                                                                    aTGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTATAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCa                                 <  2:466134/140‑1 (MQ=255)
                                                                                                    aTGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCa                                 <  2:459799/140‑1 (MQ=255)
                                                                                                    aTGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCa                                 <  1:506120/140‑1 (MQ=255)
                                                                                                    aTGTTGCAGGTAAGTTGAATATCATACTTCTCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTATAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCa                                 <  2:460505/140‑1 (MQ=255)
                                                                                                        tGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTATTATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGa                             <  2:481413/140‑1 (MQ=255)
                                                                                                            gTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTCTCAGTAAATCAAGGATACt                         >  1:347912/1‑140 (MQ=255)
                                                                                                            gTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATAAAGGATACt                         >  1:51122/1‑140 (MQ=255)
                                                                                                                  ttCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACTGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTAc                   <  1:37594/140‑1 (MQ=255)
                                                                                                                   tCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACa                  <  1:693774/140‑1 (MQ=255)
                                                                                                                    cAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACaa                 >  1:44698/1‑140 (MQ=255)
                                                                                                                                   ctgatgACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCTAGGATACTCGTTACAATTTTTAACGTACTGa  <  2:120727/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                        
CTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACAATTTTTAACGTACTGA  >  CP000730/2289191‑2289461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: