Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,454,551 G→T 4.3% M23I (ATG→ATT USA300HOU_2315 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,454,5510GT 4.3% 278.9 / 3.4 93M23I (ATG→ATTUSA300HOU_2315hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base G (45/44);  new base T (2/2);  total (47/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-02

CGGCTTGTAATATGTATAATTGTTACTAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCA  >  CP000730/2454416‑2454689
                                                                                                                                       |                                                                                                                                          
ctgCTTGTAATATGTATAATTGTTAATAAAAGATATGTTGCTTGTTACCTAATTGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGt                                                                                                                                        <  1:245071/138‑1 (MQ=255)
cGGCTTGTAAGATGTATAATTGTTACTAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGt                                                                                                                                        <  2:452512/140‑1 (MQ=255)
      gTAATATGTATAATTGTTACTAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCa                                                                                                                                  >  2:64011/1‑140 (MQ=255)
        aaTATGTATAATTGTTACTAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATCATGAAGAACAATAAACTTTCTGGTTATCAATGGGCAATGACGATTTTCGGCTACCTGGACATGGCAAGGTCGTTATCCAtt                                                                                                                                >  2:499580/1‑140 (MQ=255)
          tatGTATAATTGTTACTAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATTGCGTTATCCATTAt                                                                                                                              >  1:610774/1‑140 (MQ=255)
           atGTATAATTGTTACTAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATg                                                                                                                             >  1:254927/1‑140 (MQ=255)
           atGTATAATTGTTACTAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATg                                                                                                                             <  1:79686/140‑1 (MQ=255)
             gTATAATTGTTACTAAAAGATATTTTGCTTGTTACCAAATGGAGGTTAAATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCTTTTTgtt                                                                                                                           >  2:300117/1‑138 (MQ=255)
                 aaTTGTTACTAAAAGATATTCTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCaga                                                                                                                       >  1:443401/1‑140 (MQ=255)
                  aTTGTTACTAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCagag                                                                                                                      <  2:144298/140‑1 (MQ=255)
                   ttGTTACTAAAATATATTTTGCTTGTTTCCTAATGGAGGTTACATTTCATGAAGAACAATAAAATTTCTGGTTTTCAAGGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAgaga                                                                                                                     <  1:343816/140‑1 (MQ=255)
                   ttGTTACTAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAgaga                                                                                                                     <  1:7171/140‑1 (MQ=255)
                    tGTTACTAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGAt                                                                                                                    <  2:46966/140‑1 (MQ=255)
                     gTTACTAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTGTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCAATTATGCGCAGAGAtt                                                                                                                   >  2:458538/1‑140 (MQ=255)
                     gTTACTAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTAGCCATTATGCCCAGAGAtt                                                                                                                   >  2:366126/1‑140 (MQ=255)
                       tACTAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTTTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTc                                                                                                                 >  2:238449/1‑140 (MQ=255)
                       tACTAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTc                                                                                                                 >  1:702202/1‑140 (MQ=255)
                       tACTAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTAAATGGGCAATGAGGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATGTc                                                                                                                 >  2:620571/1‑140 (MQ=255)
                         cTAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGTCGTTATCCATTATGCTCAGAGATTTCCa                                                                                                               >  2:212182/1‑140 (MQ=255)
                         cTAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCa                                                                                                               >  2:646039/1‑140 (MQ=255)
                          tAAAAGTTATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAGATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAg                                                                                                              >  2:458429/1‑140 (MQ=255)
                          tAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGCCGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAg                                                                                                              >  2:489042/1‑140 (MQ=255)
                             aaGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAACTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCt                                                                                                           <  2:399557/140‑1 (MQ=255)
                               gttatTTTGCTTGTTACCTAATGGAGTTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCtat                                                                                                         <  1:217543/138‑1 (MQ=255)
                                   ttttGCTTGTTACCTAGTGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAAtt                                                                                                     <  2:4673/140‑1 (MQ=255)
                                   ttttGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGTTTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAAtt                                                                                                     <  2:593393/140‑1 (MQ=255)
                                   ttttGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCtctattg                                                                                                     >  2:288350/1‑137 (MQ=255)
                                   ttttGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAAtt                                                                                                     <  1:392923/140‑1 (MQ=255)
                                   ttttGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTATCTAAtt                                                                                                     >  1:128539/1‑140 (MQ=255)
                                   ttttGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGACGTTATCCATTATGCTCAGAGATTTCCAGTCTATAAtt                                                                                                     >  1:469184/1‑140 (MQ=255)
                                      tgattGTTACATAAGGGGGGGTACATTTACTGAAGAAGAAAAAGATTCCTGGCTTTCAATGGGCAATGACGATTTTCGGCTTCTGTGTCATTACAATGGCGTGATACCTTATGCGAAGAGATTTCCAGTCTATAATTTgt                                                                                                  >  2:669216/4‑140 (MQ=255)
                                       gCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGgtg                                                                                                 <  1:428701/140‑1 (MQ=255)
                                       gCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTATGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGgtg                                                                                                 >  1:712137/1‑140 (MQ=255)
                                       gCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTACGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAACTgagc                                                                                                 >  2:540664/1‑137 (MQ=255)
                                       gCTTGTGACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGgtg                                                                                                 <  1:13627/140‑1 (MQ=255)
                                             tACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATCAGGATTGTCGTCTTCTGTGTCATTACAATGGCGTGATCCAGTATGCGCAGAGATTTCCAGTCTATAATTGGTGTCAAac                                                                                           <  2:206239/140‑1 (MQ=255)
                                              aCCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAaca                                                                                          >  2:589936/1‑140 (MQ=255)
                                                  aaTGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACttt                                                                                      >  2:219134/1‑140 (MQ=255)
                                                  aaTGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACttt                                                                                      <  1:518324/140‑1 (MQ=255)
                                                    tGGAGGTTACATATAATGAATAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTGATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTtat                                                                                    <  1:616132/140‑1 (MQ=255)
                                                      gaagTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTatat                                                                                  <  2:141428/137‑1 (MQ=255)
                                                      gAGGTTACAGATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCTTTATCCATTATGCTCAGATATTTCCAGTCTATAATTGGTGTCAAACACTTTatat                                                                                  <  2:220755/140‑1 (MQ=255)
                                                       aGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATAtt                                                                                 >  1:152221/1‑140 (MQ=255)
                                                        ggTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTAGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATAttt                                                                                >  1:570253/1‑140 (MQ=255)
                                                           tACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGaa                                                                             >  2:638110/1‑140 (MQ=255)
                                                            aCATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAg                                                                            <  2:305744/140‑1 (MQ=255)
                                                            aCATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAg                                                                            >  2:488592/1‑140 (MQ=255)
                                                             tatatAATGATGAACAATAAAATTTTTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGt                                                                           <  1:308772/139‑1 (MQ=255)
                                                             cATATTATGAAGAACATTAAAATTTCTTGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGt                                                                           <  1:33345/140‑1 (MQ=255)
                                                                ataATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTAc                                                                        <  2:405582/140‑1 (MQ=255)
                                                                 taATGAAGAACAATAAAATTTCTGGTTTTCAATCGGCAATGACGATATTCGACTTCTTTTTCATAACAATGGCGTCATCTATTATGCTCTGAGTTTACCAGTCTAGAAGTTGTGTCTAACATTTTATTTTTGTATTTACa                                                                       <  2:355293/140‑1 (MQ=255)
                                                                     gaagcaCAATAAGTGTTCTGGTTTTCAATGGGTAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATc                                                                   <  2:112101/140‑1 (MQ=255)
                                                                        gaaCAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTAATTACAATGGCGTTATCCATTATGCTCAGAGATTTCAAATCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATATAg                                                                >  2:244921/1‑140 (MQ=255)
                                                                         aaccacaaaaTTTCTTGTTTTCCATGGGCCATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGTTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGc                                                               <  2:415360/134‑1 (MQ=255)
                                                                         aaCAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGCTCTAGc                                                               >  1:487830/1‑140 (MQ=255)
                                                                            aaaaaaaTTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCAcc                                                            <  2:565751/137‑1 (MQ=255)
                                                                            aaTAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCAcc                                                            <  2:104571/140‑1 (MQ=255)
                                                                            aaTAAAATTTCTGGATTTCAATGGGCAATGACGAATATCGACTTCTTTGTCATCACAATGGCGTTATCCATTATGCACCAAGATTTCAAGTAGATAATTCGGATCACACACTTTCTATTTGTAGTTACAGATATAGccac                                                            >  2:55299/1‑137 (MQ=255)
                                                                             aTAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCa                                                           >  1:146818/1‑140 (MQ=255)
                                                                             aTAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTAAAGACATAGccgca                                                           <  2:608706/140‑5 (MQ=255)
                                                                             aTAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAATTACACATCTAGCAACa                                                           <  2:608633/140‑1 (MQ=255)
                                                                              tAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTCCAATGGCGTTATCCATTATGCTCAGAGATTTCCAGACGATAATTAGTGTCAAACACTTTAAATTTGAAGTTACAAATCTAGCACCAt                                                          >  1:639920/1‑140 (MQ=255)
                                                                              tAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCNTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGAACTTGCACCAt                                                          >  2:374710/1‑140 (MQ=255)
                                                                              tAAAATTTCTGGTTTTCAATGGGCAATGACGAGTTTCGTCTTCTTGGTCATTACAATGGCGTTATCCCTTAGTCGCAGAGATTCCTAATCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCAt                                                          <  1:169941/140‑1 (MQ=255)
                                                                               aaaaTTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTAACAGATCTAGCACCAtt                                                         >  2:464270/1‑140 (MQ=255)
                                                                                 aaTTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTaa                                                       >  2:103639/1‑140 (MQ=255)
                                                                                   tttCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACGATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAAtt                                                     <  2:496703/140‑1 (MQ=255)
                                                                                    ttCTGGTTTTCAATGGTCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATtg                                                    <  1:470889/140‑1 (MQ=255)
                                                                                      cTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATTCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATtgct                                                  <  1:387555/140‑1 (MQ=255)
                                                                                      cTGGTTTTCAAGGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGCTGTCAATCACTTTATATTTGAAGTTACAGATCTAGCACCATTAATtgct                                                  <  2:119536/140‑1 (MQ=255)
                                                                                       tGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATTGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCAt                                                          <  1:226476/131‑1 (MQ=255)
                                                                                       tGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATTGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCAt                                                          >  2:226476/1‑131 (MQ=255)
                                                                                        ggTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATTGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATtgctgc                                                <  2:610774/140‑1 (MQ=255)
                                                                                        ggTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATtgctgc                                                >  2:386089/1‑140 (MQ=255)
                                                                                         gTTTTCAATGGTCAATGACGATTTCCGTCTTCTTTGTCATTACAATGGCGGTATCCAGTATGCTCTGAGATTTCCAGTGTATAAGTTGTGTCTAACATTTTAGATGTGAAGTTACAGATCTAGCACCATTAAGTTCTGCa                                               <  2:197671/140‑1 (MQ=255)
                                                                                          ttttCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCaa                                              >  1:382657/1‑140 (MQ=255)
                                                                                               aaTGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCAtt                                         >  2:344631/1‑140 (MQ=255)
                                                                                                 tGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTg                                       >  1:255023/1‑140 (MQ=255)
                                                                                                   ggCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACATATCTAGCCCCATCAATTTCTGCAATCCTTTGta                                     >  1:131115/1‑140 (MQ=255)
                                                                                                      aaTGCCTATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATAc                                  <  2:560465/140‑1 (MQ=255)
                                                                                                      aaTGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATAc                                  <  2:404083/140‑1 (MQ=255)
                                                                                                         gACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTATATAATTGGTGTCAAACACTTTATATACGAAAATATATATCTAGCACCATAAATTGCTGCAATCATTTGTATACTCg                               >  1:343577/1‑140 (MQ=255)
                                                                                                              ttttCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGCTCTAGCACCAGTAATTGCTGCAATCATTTGTATACTCGTTTTc                          >  1:198003/1‑140 (MQ=255)
                                                                                                              ttttCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTc                          >  1:103812/1‑140 (MQ=255)
                                                                                                                 tCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCaaa                       <  1:488592/140‑1 (MQ=255)
                                                                                                                      ttcttTGTCATTACAGTGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATaa                  >  1:540497/1‑140 (MQ=255)
                                                                                                                             ttaTTACAGTGGCGTTATCCATCAAGCTCCGAGATTTCCAGTCTATAATTGGTGTCAAATACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTAGTTTCCAAATATAAAAAGGTc           <  1:374348/138‑1 (MQ=255)
                                                                                                                             tCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTc           >  2:44758/1‑140 (MQ=255)
                                                                                                                              cATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTcc          <  1:589936/140‑1 (MQ=255)
                                                                                                                                     aTGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGc   <  1:406489/140‑1 (MQ=255)
                                                                                                                                     aTGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGc   <  1:120976/140‑1 (MQ=255)
                                                                                                                                      tGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTTGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCa  <  2:429603/140‑1 (MQ=255)
                                                                                                                                      tGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCa  >  2:523238/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                          
CGGCTTGTAATATGTATAATTGTTACTAAAAGATATTTTGCTTGTTACCTAATGGAGGTTACATATAATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCA  >  CP000730/2454416‑2454689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: