Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 221,865 C→A 7.4% G475V (GGC→GTC)  USA300HOU_0203 ← PTS family glucose/glucoside (glc) porter component IIABC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730221,8650CA 7.4% 204.4 / 5.4 85G475V (GGC→GTC) USA300HOU_0203PTS family glucose/glucoside (glc) porter component IIABC
Reads supporting (aligned to +/- strand):  ref base C (44/32);  new base A (3/3);  total (48/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-01

TCTTTATCGTCTTCCATAGTAGTAGGATTTTCTACTACTTGACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCTTCTAATACTGCATATGGTAATTCAGTT  >  CP000730/221727‑222002
                                                                                                                                          |                                                                                                                                         
tCTTTATCGTCTTCCATAGTAGTAGGATTTTCTACTACTTGACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGcc                                                                                                                                          <  1:303034/140‑1 (MQ=255)
tCTTTATCGTCTTCCATAGTAGTAGGATTTTCTACTACTTGACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGcc                                                                                                                                          >  1:698710/1‑140 (MQ=255)
 cTTTATCGTCTTCCATAGTAGTAGGATTTTCTACTACTTGACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCt                                                                                                                                         >  1:508241/1‑140 (MQ=255)
   ttATCGTCTTCCATAGTAGTAGGATTTTCTACTACTTGACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTaa                                                                                                                                       >  1:697396/1‑140 (MQ=255)
   ttATCGTCTTCCATAGTAGTAGGATTTTCTACTACTTGACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAAATTTAGGACCAAAAATTGCTTGCATATTATTGCCGAGTTCTAATACACCAGATGCGCCTaa                                                                                                                                       >  1:625508/1‑140 (MQ=255)
    tATCGTCTTCCATAGTAGTAGGATTTTCTACTACTTGACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTTCATATTATTGCCGACTTCTAATACACCAGATGCGCCTaaa                                                                                                                                      >  2:115727/1‑140 (MQ=255)
      tagTCTACCATAGAAGTAGGATTTTATACTACTTAACCATTAATCATATGTAGCAATTAATGTTTGATTTGGTCAGATTTAGGACAAAAAATAGCTTGCATATTATTGCCGACTTCTAATACACCAGATTCGCCTaaaac                                                                                                                                    >  2:58600/3‑138 (MQ=255)
        gTCTTCCATCGTAGAAGGATTTTCTACTACTTGACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGGCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCTGACTTCTAATCAACCAGCTGCGCCTAAATCtt                                                                                                                                  >  2:85064/1‑140 (MQ=255)
         tCTTCCATAGTAGTAGGATTTTCTACTACTTGACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCttt                                                                                                                                 >  1:548037/1‑140 (MQ=255)
                    agtagGATTTTCTACTACTTGACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGaa                                                                                                                      >  1:50148/1‑140 (MQ=255)
                     gtagGATTTTCTACTACTTGACCATTCATAATCTTTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAAc                                                                                                                     <  1:307415/140‑1 (MQ=255)
                       agaaaatcctactaCTTGACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACAt                                                                                                                   >  1:89432/9‑140 (MQ=255)
                        ggtttttttACTATTTGACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATc                                                                                                                  <  1:37940/137‑1 (MQ=255)
                         gtttttCTACTACTTGACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGAGTACTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCa                                                                                                                 <  1:684101/138‑1 (MQ=255)
                           ttttCTACTACTTGACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACAAGATGCGCATAAATCTTTCAAACTAGGACAATCAAc                                                                                                               >  1:711394/1‑140 (MQ=255)
                           ttttCTACTACTTGACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGATTGCACAAAAAGGGCGACTTCTAATACACCAGATGCGCCTACATCTTTCAAACCAGGAACATCAAc                                                                                                               >  1:528413/1‑140 (MQ=255)
                           ttttATACGACTTGAGCATTGATAATATGTTGAATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCCGCTACATCTTTCAAAACAGGAACAGCAAc                                                                                                               >  2:90372/1‑140 (MQ=255)
                              tCTACTACTTGACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACttt                                                                                                            >  2:25436/1‑140 (MQ=255)
                                  ctactTGACCATTCATAATCTTTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGAt                                                                                                        >  2:549709/1‑140 (MQ=255)
                                  ctactTGACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGAGGCGCCTAAATCTTTCAAACCAGGAACATTAACTTTAGAt                                                                                                        >  1:564756/1‑140 (MQ=255)
                                  ctactTGACCATTCATAATCTAGCACTTTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGAt                                                                                                        >  1:173857/1‑140 (MQ=255)
                                   tactTGACCATTCATAATCTGTTGCATTTCCTGTTTGAGTTGGTCAGATTTAGGACCAAAAATTGGTTGCATATTATTGCAGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCGGGAACATCACCtgtcaatt                                                                                                       >  1:208079/1‑133 (MQ=255)
                                        gACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCg                                                                                                  <  2:87043/140‑1 (MQ=255)
                                         aCCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGt                                                                                                 <  1:25436/140‑1 (MQ=255)
                                              tCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACt                                                                                            >  1:301945/1‑140 (MQ=255)
                                               cATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCCAAAATTGCTTGCATATTATTGCCGACTTCTAGTACACCAGATGCGCCTAAATGTTTCAAACCAGGAACATCAACTTTAGATTTGTCGCTAACtt                                                                                           >  2:38411/1‑140 (MQ=255)
                                                    tCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAAATTTGTCGTTAACTTCAaca                                                                                      >  1:323503/1‑140 (MQ=255)
                                                    tCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATAAACTTTAGATTTGTCGTTAACTTCcaca                                                                                      >  2:411271/1‑140 (MQ=255)
                                                       gtggCATTTCATGTTTGACTTCGTCAGATTTATGACAAAAAATTGCTTGCATACTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGt                                                                                   <  1:710620/137‑1 (MQ=255)
                                                       gTTGCATTTCATGTTTGTTTTGGTCAGATTTAGGCCCAAAAATTGCTTGCATACTATTGCCGACTTCTAATACACCAAATGCGGCTACATCTTTCAATCCAGGAACATCAATTTTAGATTTGTCGTGAACTTCAACACGt                                                                                   <  2:607505/140‑1 (MQ=255)
                                                       gTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGt                                                                                   >  2:218133/1‑140 (MQ=255)
                                                         tGCATTTCATGTTTTATTTGGTTAGATTTAGGACCAAATATTGCTTGCATATTATTGCCGACTTCTAATACACCAGTTGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCatacgtcta                                                                                 <  1:457452/140‑9 (MQ=255)
                                                         tGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAACATTTCTTTCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTCCAAACCAGTATCATCAACTTTAGATTTGTCGTTAACTTAAACACGtcg                                                                                 <  2:708855/140‑3 (MQ=255)
                                                         tGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAg                                                                                 >  2:130772/1‑140 (MQ=255)
                                                          gCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGACTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGa                                                                                >  1:92950/1‑140 (MQ=255)
                                                          gCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGACTAAATCTTTCAAACCAGGAACATCAACTTTAAATTTGTCGTTAACTTCAACACGTAGa                                                                                >  1:179856/1‑140 (MQ=255)
                                                          gCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATAGTATTGCCGACTTCTAATACACCAGATGCGACTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGa                                                                                >  2:535389/1‑140 (MQ=255)
                                                            aTTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTAAACACGTAGACg                                                                              >  2:42777/1‑140 (MQ=255)
                                                               tCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACgtgt                                                                           >  2:591510/1‑140 (MQ=255)
                                                                 aTGTTTGATTTGGTCAGATTTAGGACCAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGAt                                                                        <  1:92893/140‑1 (MQ=255)
                                                                  tGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGAt                                                                        <  1:570129/140‑1 (MQ=255)
                                                                   gTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGAATTGTCGTTAACTTCAACACGTAGACATTTGATa                                                                       >  1:341298/1‑140 (MQ=255)
                                                                          ttGTTCAGATTTAGTACCAAAAATTGCTGGCATATTATTGCCGACTTCGAATACACCAGATGCTCCTAAATCTTTCAAACCAGGAACAGCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGt                                                                <  1:327332/140‑1 (MQ=255)
                                                                              tCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTaa                                                            >  1:139823/1‑140 (MQ=255)
                                                                               cAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTATaaa                                                           >  2:725827/1‑140 (MQ=255)
                                                                                  aTTTATGACCAAAAATTGCTTGCATATGATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAaatgt                                                        <  2:10401/140‑1 (MQ=255)
                                                                                       ggACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTTTAaatgtttaat                                                   >  2:241761/1‑140 (MQ=255)
                                                                                       ggACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAaatgtttact                                                   >  2:126068/1‑138 (MQ=255)
                                                                                         aCCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAaatgtttaatgt                                                 <  2:600213/140‑1 (MQ=255)
                                                                                          ccAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAaatgtttaatgtt                                                >  2:604432/1‑140 (MQ=255)
                                                                                           cAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAaatgtttaatgttt                                               <  1:16272/140‑1 (MQ=255)
                                                                                             aaaaTTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCATACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGtttgc                                             <  1:142686/140‑1 (MQ=255)
                                                                                              aactttgttgcATATTATTGCCGACTTCTAATATCCCAGATGCTCCCATACCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGtttgct                                            <  2:218519/133‑1 (MQ=37)
                                                                                               aaTTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTCGACGTGTGATACAAGCGTCTAAATGTTTAATGtttgctt                                           >  2:213209/1‑140 (MQ=255)
                                                                                                  tgcttgcATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCATGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGtttgctttgc                                        <  2:39541/140‑1 (MQ=255)
                                                                                                      tgcAAATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACCTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCAcc                                    >  2:441550/1‑140 (MQ=255)
                                                                                                       gcATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACACGCGTCAAAATGTTTAATGTTTGCATTGCCAccc                                   <  1:533729/140‑1 (MQ=255)
                                                                                                             tattGCCGACTTCTAATACACCAGCTGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAATCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCt                             <  2:50148/140‑1 (MQ=255)
                                                                                                              attGCCGACTTCTAATACACCAGATGCGCCTACATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCGAAATGTTTAAAGTTTGCTTTGGCACACATAGCta                            >  2:617207/1‑139 (MQ=255)
                                                                                                              attGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCtt                            <  1:218133/140‑1 (MQ=255)
                                                                                                              attGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCtt                            >  2:560194/1‑140 (MQ=255)
                                                                                                               ttGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCTTc                           <  2:606212/140‑1 (MQ=255)
                                                                                                                ttcctaCTTCTAATCCCCCGGATGCGCATAAATCTTTTAAACCACGAACATCAACTTTAGATGTGGCCTTAAGTTAAAGACGTAAACGTGTGTTTCAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCTTAtcttct                          <  2:43064/135‑1 (MQ=255)
                                                                                                                 gCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCATACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGATTCTa                         >  1:143304/1‑140 (MQ=255)
                                                                                                                 gCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAATATTAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCTTCTa                         <  1:96692/140‑1 (MQ=255)
                                                                                                                     aCTTTTAATACACTCGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCTTCTAATAc                     <  1:58600/140‑1 (MQ=255)
                                                                                                                     aCTTCTTATACCCCATTTGCCCCTAAATCTTTTAAATCAGGAGCATCATCTTTCTCTTTGTCGTTAACTTCAACACGTAGACATGTTATTCAAGCGTCTAAATGTTTAATTTTTGCTTTGTCACCCATAGCTTCTAATAc                     <  2:310536/140‑1 (MQ=255)
                                                                                                                       ttCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCACCACGTAGACGTGTGATACAAGAGTCTAAATGTTTAATGTTTGCTTTGCCACCGATAGCTTGTAATACTg                   >  1:58915/1‑140 (MQ=255)
                                                                                                                        tCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCTTCTAATACTGc                  >  1:476184/1‑140 (MQ=255)
                                                                                                                        tCTAATACACCAGATGCGACTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCTTCTAATACTGc                  <  2:92950/140‑1 (MQ=255)
                                                                                                                        tCTAATACACCAGATGCGACTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCTTCTAATACTGc                  <  2:179856/140‑1 (MQ=255)
                                                                                                                        tCTAATACACCAGATGCGACTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGACACCCATAGCTTCTAATACTGc                  <  1:535389/140‑1 (MQ=255)
                                                                                                                           aaTACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACCCGTAAACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCAACCGTTACTTCTAATACTGCata               >  1:93262/1‑140 (MQ=37)
                                                                                                                           aaTACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCTTCTAATACTGCata               >  1:293171/1‑140 (MQ=255)
                                                                                                                            aTACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATCTTTGCTTTGCCACCCACAGCATCTAATAATGcccaa              >  1:248369/1‑136 (MQ=255)
                                                                                                                            aTACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACGTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCTTCTAATACTGCatat              <  1:133429/140‑1 (MQ=255)
                                                                                                                             tACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATCATAGAGAATAAATTTTTAATGTTGCCTTGTCCACCCATAGCTTCTAATTCTGTATTTg             <  2:697396/140‑1 (MQ=255)
                                                                                                                             tACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCTTAGCTTCTAATACTGCATATg             >  2:311166/1‑140 (MQ=255)
                                                                                                                             aacacCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCGTCTAATACTGCATATg             <  2:625508/139‑1 (MQ=255)
                                                                                                                              acacCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGCTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCTTCTAATACTGCATATgg            >  1:485332/1‑140 (MQ=255)
                                                                                                                               ccccAGATGCGCCTAAATCTTTCAAACCACGAACATCAACTTTATATTTGTCGTTAACTTCAACATGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCTTCTAATACTGCATATGGt           <  1:18620/138‑1 (MQ=255)
                                                                                                                                   agctGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTCGTCGTTAACTTCAACACGTAGACGTGTGATGCAAAAGTCTAAAAGTCTAAGGTTTGCTCTGCCGCCCATAGCTTCTAATACTGCAAACGGTAAtt       <  2:697389/137‑1 (MQ=255)
                                                                                                                                    gATGCGCCTAAATCTATCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCTTCTAATACTGCATATGGTAATTc      >  1:539387/1‑140 (MQ=255)
                                                                                                                                     aTGCGGCTAATTCTTTCAAACCAGGAACATCCACTTTCGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACCGGCGTTTAGATGTTTAATGTTTTTTTTGCCACCCATAGCTTCTAATACTGCATATGGTAATTCa     <  2:645690/140‑1 (MQ=255)
                                                                                                                                     aTGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACATTTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCTTCTAATACTGCATATGGTAATTCa     >  1:31722/1‑140 (MQ=255)
                                                                                                                                      tGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCTTCTAATACTGCATATGGTAATTCAg    <  1:549709/140‑1 (MQ=255)
                                                                                                                                      tGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTCTGCCACCCATAGCTTCTAATACTGCATATGGTAATTCAg    <  2:298923/140‑1 (MQ=255)
                                                                                                                                       gcgcCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTACCTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCGTTGCCACCCATAGCTTCTAATACTGCATATGGTAATTCAGt   >  1:28565/1‑140 (MQ=255)
                                                                                                                                       gcgcCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCTTCTAATACTGCATATGGTAATTCAGt   <  2:301945/140‑1 (MQ=255)
                                                                                                                                       gcgcCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGATTCTAATACTGCATATGGTATTTCAGt   >  1:78/1‑140 (MQ=255)
                                                                                                                                        cgcCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCTTCTAATACTGCATATGGTAATTCAGtt  >  2:635596/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                         
TCTTTATCGTCTTCCATAGTAGTAGGATTTTCTACTACTTGACCATTCATAATCTGTTGCATTTCATGTTTGATTTGGTCAGATTTAGGACCAAAAATTGCTTGCATATTATTGCCGACTTCTAATACACCAGATGCGCCTAAATCTTTCAAACCAGGAACATCAACTTTAGATTTGTCGTTAACTTCAACACGTAGACGTGTGATACAAGCGTCTAAATGTTTAATGTTTGCTTTGCCACCCATAGCTTCTAATACTGCATATGGTAATTCAGTT  >  CP000730/221727‑222002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: