Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,814 Δ1 bp 25.6% intergenic (+45/+63) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8140A.25.6% 286.7 / 116.7 125intergenic (+45/+63)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (47/44);  new base . (11/21);  total (59/66)
Fisher's exact test for biased strand distribution p-value = 1.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

TATGCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTAT  >  CP000730/35674‑35953
                                                                                                                                            |                                                                                                                                            
tatGCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATa                                                                                                                                              <  1:397435/141‑1 (MQ=255)
    cAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAg                                                                                                                                          <  1:638975/141‑1 (MQ=255)
     aaaTTTGTCGGAATTTAAAGATGTAGCTGATCTTATTGACTGAGGTTTTAAAGTAGAAGCTATTCAGTATAAAGTTGTTGGCGAAAAATGATTACTTGAACGAGAGCTAAAATAAAATTGTGATCTAATAATAATAGAAgg                                                                                                                                         <  2:172124/141‑1 (MQ=255)
     aaaTTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAgg                                                                                                                                         <  2:54566/141‑1 (MQ=255)
         tattCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAAAAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCt                                                                                                                                     <  2:334676/141‑1 (MQ=255)
          attCTGAATTTAAAGATGTATCTGATCTCATTGACAAAGTATTTAATGTATAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATGACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGGTCTg                                                                                                                                    >  2:168067/1‑141 (MQ=255)
            tCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGtt                                                                                                                                  >  1:341772/1‑141 (MQ=255)
              tGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGc                                                                                                                                <  1:620440/141‑1 (MQ=255)
              tGAATTTAAAGATGGAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGc                                                                                                                                <  2:364720/141‑1 (MQ=255)
                  tttAAAGATATAGACGATCTCATTGACACAGTATTTAACCTAGCAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACCTGGGTTAAAAGAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAg                                                                                                                            <  1:57398/141‑1 (MQ=255)
                      aaGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCCAAGTaaa                                                                                                                        >  2:359423/1‑141 (MQ=255)
                      aaGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTGGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTACTGTTGCAAGGTaaa                                                                                                                        >  2:255109/1‑141 (MQ=255)
                       aGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTcaaa                                                                                                                       >  1:230072/1‑141 (MQ=255)
                       aGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaaa                                                                                                                       >  2:535159/1‑141 (MQ=255)
                       aGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaaa                                                                                                                       >  1:308879/1‑141 (MQ=255)
                           gTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAat                                                                                                                   >  2:393780/1‑141 (MQ=255)
                           gTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCAAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAat                                                                                                                   >  2:107620/1‑141 (MQ=255)
                             aGATTATCTCATTGACAAAGTATTTAATGTAGAAGTTATTCAATTTAAAGTTTTTCGCGAAAAATGAATACTTGAACAAGATCTAAAATAAAATTGTTATCTAATAAAAATAGAAGGTTTTATTGCAAATTAAAAAAatat                                                                                                                 <  2:289650/141‑1 (MQ=255)
                                  atCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAat                                                                                                                   >  1:585516/1‑134 (MQ=255)
                                       aTTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTGAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAAacac                                                                                                       >  2:496999/1‑141 (MQ=255)
                                        ttGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACt                                                                                                      <  1:217905/141‑1 (MQ=255)
                                          gACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTATTTGGCGAAAAATGAGTACTTGAAGAAGAGCGAAAATAAAATTGTGATCTAATAAAAATAGAAGTTTCTGTTGCAACGTAAAAAAATATAGCTAACCACTaa                                                                                                    >  2:595467/1‑141 (MQ=39)
                                              aaGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAGGAGCTAAAATAGAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAGAAAAATATAGCTGACCACTAATTTa                                                                                                >  1:548228/1‑141 (MQ=255)
                                               aGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTAt                                                                                               >  1:504750/1‑141 (MQ=255)
                                                gTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATc                                                                                              >  2:486613/1‑141 (MQ=255)
                                                     tAACGTTGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAAGAGAAGGTTCTGTTTCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTc                                                                                         <  2:273415/141‑1 (MQ=255)
                                                      aaTGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCa                                                                                        <  1:303465/141‑1 (MQ=255)
                                                      aaTGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCa                                                                                        >  1:600351/1‑141 (MQ=255)
                                                      aaTGAAGAAGCTATACAATATAAAGTTTTTGTCGAAAAATGATGACTTGAACAAGAGCACAAAAAAAAATGTGATCTAATAAAAATAGAAGAATCAGTAGCAAGCTAAAAAAATATAGCAAACCACCaaataaccacagca                                                                                        >  2:151499/1‑129 (MQ=255)
                                                       aTGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAg                                                                                       >  2:577636/1‑141 (MQ=255)
                                                             aaGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCg                                                                                 >  1:567723/1‑141 (MQ=255)
                                                             aaGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCg                                                                                 >  1:209768/1‑141 (MQ=255)
                                                              aGCTATTCAGTATAAAGTTGTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAGATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                >  1:400804/1‑141 (MQ=255)
                                                                  aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGATTaa                                                                            >  2:610292/1‑141 (MQ=255)
                                                                    tCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATATAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATATCAGTGTTCGCTTacgt                                                                          >  2:27577/1‑138 (MQ=255)
                                                                     cAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTTTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCCGTGTTCGCTTAACtt                                                                         >  1:291867/1‑141 (MQ=255)
                                                                        tataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCt                                                                      <  1:166699/141‑1 (MQ=255)
                                                                          taAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATACAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTTTCATGTCAGTGTTCGCTTAACTTGCTAg                                                                    <  1:430572/141‑1 (MQ=255)
                                                                          aaaaGTTTTGGGTGAAAAATGATTACATGAACATGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAg                                                                    <  2:375255/140‑1 (MQ=255)
                                                                            aaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAGTTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCa                                                                  >  1:521302/1‑141 (MQ=255)
                                                                            aaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATGGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCa                                                                  >  2:571357/1‑141 (MQ=255)
                                                                            aaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTTCTAGCa                                                                  >  2:200779/1‑141 (MQ=255)
                                                                            aaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCa                                                                  >  1:662746/1‑141 (MQ=255)
                                                                             aGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGCAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCCCTAATTTCTAGTGTCAGTGTTCCATTaagcggcgagcat                                                                 >  2:69154/1‑130 (MQ=25)
                                                                              gTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatg                                                                >  2:101004/1‑141 (MQ=255)
                                                                              gTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCAAGTCGGTGTTCGCTTAACTTGATAGAatg                                                                >  1:13971/1‑141 (MQ=255)
                                                                                    ggCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTa                                                         >  2:534511/1‑141 (MQ=255)
                                                                                     gCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTaa                                                        >  2:122052/1‑141 (MQ=255)
                                                                                     gCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTaa                                                        >  1:568771/1‑141 (MQ=255)
                                                                                           aaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGt                                                  <  2:341772/141‑1 (MQ=255)
                                                                                                  aCTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATgg                                           <  1:147728/141‑1 (MQ=255)
                                                                                                   cTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGc                                          <  2:311479/141‑1 (MQ=255)
                                                                                                   cTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGc                                          <  1:26438/141‑1 (MQ=255)
                                                                                                   cTTGAACAAGAGCAAAAATAAAATTGTGAACTAACAAAAATAGAAATTTCAGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGc                                          <  2:78262/141‑1 (MQ=17)
                                                                                                        aCAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaaaa                                     <  1:541593/141‑1 (MQ=255)
                                                                                                           agagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                  >  1:16745/1‑141 (MQ=255)
                                                                                                            gagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCg                                 >  2:507716/1‑141 (MQ=255)
                                                                                                             agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGGATGGCGAAAATCCGt                                <  1:496999/141‑1 (MQ=255)
                                                                                                             agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGt                                >  2:198377/1‑141 (MQ=255)
                                                                                                                      aaaatTGTGATCTAATAAAACTAGAAGGTTCTGTTGCAAAATAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                       <  2:603107/141‑1 (MQ=255)
                                                                                                                      aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGGTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                     <  1:349442/129‑1 (MQ=11)
                                                                                                                       aaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                      <  1:290451/141‑1 (MQ=255)
                                                                                                                         atTGTTATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTCATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                    <  2:483979/141‑1 (MQ=255)
                                                                                                                         agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATTTCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                  <  1:450627/132‑1 (MQ=11)
                                                                                                                         agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                  <  1:92621/132‑1 (MQ=11)
                                                                                                                         agagtattttaataaAAATGATGGGTCTGGTGCAAAGTAAAAAATATAGCTAATCACTATTATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCGAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                  <  2:69897/132‑1 (MQ=11)
                                                                                                                          gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATGGCTGATCACTACTATATCATGTCGGTGTTCGTTTAGCTTGCTGGCATGATGCTAAGCGCGTGGCATGGCGAAAATCCGTAGGTCTGAAGAGAcc                 <  2:25692/133‑1 (MQ=2)
                                                                                                                           tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                  <  1:535159/141‑1 (MQ=255)
                                                                                                                           tgtgATCTAATAAAAATAGAAGGTTATGTTGCAAAGTAAAAAAGTATAGCTAACCCCTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                  <  1:253781/141‑1 (MQ=255)
                                                                                                                           ggggATCTAATAAAAATAGAAGGTTATGGTGAAAAGTAAAAAAAGATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTGGCTAGCATGATTCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                  <  2:395592/138‑1 (MQ=14)
                                                                                                                           agtattttcataaAAATGATGGTTCTGTTGTAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                <  1:459642/132‑1 (MQ=11)
                                                                                                                           agtattttaataaAAATCATGGTGCTGTTGCAAAGTAAAAAACATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                <  2:22969/134‑1 (MQ=11)
                                                                                                                            gtgATCTAATTAAAATCGCCGGTTCGGTGGGAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCGGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                 <  2:636600/141‑1 (MQ=11)
                                                                                                                            gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTTGTGTATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg               <  1:654593/135‑1 (MQ=11)
                                                                                                                            gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg               <  1:409173/135‑1 (MQ=11)
                                                                                                                            gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATCCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg               <  2:225966/135‑1 (MQ=11)
                                                                                                                            gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAAATTGCTAGCTTGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg               <  2:305666/135‑1 (MQ=11)
                                                                                                                             tgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                <  2:209768/141‑1 (MQ=255)
                                                                                                                             tgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                <  1:534511/141‑1 (MQ=255)
                                                                                                                             tgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                <  2:567723/141‑1 (MQ=255)
                                                                                                                             tattttaataaACATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTAGTGGCATGGCGAATATCTGTAGATCTGGAGAGAACTgg              >  1:166184/6‑140 (MQ=11)
                                                                                                                             tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc              <  1:6332/136‑1 (MQ=11)
                                                                                                                              attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg             <  2:437578/137‑1 (MQ=11)
                                                                                                                              attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg             <  2:275314/137‑1 (MQ=11)
                                                                                                                              atttaaataaAAATGATGGTTCTTTTGCAAAGTAAAAAATAAAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg             <  1:34827/136‑1 (MQ=11)
                                                                                                                               ttttaataaAAATGATTGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTCTGAAGAGACCTGCgg            >  1:332441/4‑141 (MQ=11)
                                                                                                                               ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg            >  1:510624/4‑141 (MQ=11)
                                                                                                                               ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATAGAGCTAAACACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg            >  2:433434/4‑141 (MQ=11)
                                                                                                                               aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc              >  1:618958/1‑141 (MQ=255)
                                                                                                                               aTCTAATAAAAAGATGAGGTTCTGTTGGAAAGTAAAAAAATATAGCTAACCACTCATTTATCAAGTCAGTGGTCGCTTAACGTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc              <  2:237962/141‑1 (MQ=11)
                                                                                                                                tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGGGACCTGCGGt           >  1:12890/3‑141 (MQ=11)
                                                                                                                                tCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg             <  1:260334/141‑1 (MQ=255)
                                                                                                                                tCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg             <  2:346562/141‑1 (MQ=255)
                                                                                                                                 ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt          >  1:201000/2‑141 (MQ=11)
                                                                                                                                 ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt          <  2:19666/140‑1 (MQ=11)
                                                                                                                                  taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGttt         >  2:295852/1‑140 (MQ=11)
                                                                                                                                   aataaAAATGATGGTTCTGTAGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTTGTTTAAATAACAACAAAATGGCTAATTTCGTGGCATGGCGAAAATCCGTAGGACTGAAGAGAGCTGCGGATCt        >  1:154361/1‑141 (MQ=2)
                                                                                                                                    ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt       >  1:439334/1‑141 (MQ=11)
                                                                                                                                     taaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt        <  1:144924/141‑1 (MQ=38)
                                                                                                                                           aaGAATGTTCTGTTGCAAAGTAAAAAAATCTAGCTAACCACTAATTTATCATGTCAGTGTCCGCTTAACTTTCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat  <  2:58412/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TATGCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTAT  >  CP000730/35674‑35953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: