Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,087 A→G 59.8% C155C (TGT→TGC USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,0870AG59.8% 339.9 / inf 678C155C (TGT→TGCUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (147/125);  new base G (230/173);  total (378/300)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 681 total aligned reads displayed.

TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAT  >  CP000730/35947‑36226
                                                                                                                                            |                                                                                                                                           
ttcttATATAGAGCGTAAATACATTCAATACGTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGa                                                                                                                                             <  2:556779/138‑1 (MQ=255)
  tttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATGTTTCGATGTACAATGACa                                                                                                                                           >  1:338743/1‑141 (MQ=255)
   ttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAg                                                                                                                                          >  1:440633/1‑141 (MQ=255)
   ttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAg                                                                                                                                          >  1:578755/1‑141 (MQ=255)
     atataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTc                                                                                                                                        <  2:20982/141‑1 (MQ=255)
     atataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTc                                                                                                                                        <  1:134253/141‑1 (MQ=255)
     atataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCATCCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTc                                                                                                                                        >  1:182833/1‑141 (MQ=255)
       ttatagCGTAAATACCCTCAATTTCTTTTAAAGTATTTGTTGCTGCTTTTATCCTTTGATATTTTGTTTTTCTTACTTTAATATGACGGTGATCTTGCTCAATCAGGTTATTCAGATATTTCGATGTACATTGtcagtcag                                                                                                                                      <  2:86640/137‑1 (MQ=14)
            gCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgttta                                                                                                                                 <  2:505831/141‑1 (MQ=255)
              gTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATGTCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGGTATTCAGATATTTGGATGTACAATGACAGTAGGgtttaag                                                                                                                               >  2:239161/1‑141 (MQ=14)
              gTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag                                                                                                                               >  2:402369/1‑141 (MQ=255)
               tAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagt                                                                                                                              >  2:230437/1‑141 (MQ=255)
                aaaTACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtt                                                                                                                             <  1:101004/141‑1 (MQ=255)
                 aaTACATTCAATACCTTTTAAAGTATTCTTTGGCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATCACGGTGCTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAGTGCAGTCAGgtttaagttt                                                                                                                            >  1:90592/1‑141 (MQ=11)
                 aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                                                                                                            >  1:206566/1‑141 (MQ=255)
                   tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCCTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa                                                                                                                          >  1:278653/1‑141 (MQ=255)
                   tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACACTGGCAGTCAGgtgtaagtttaa                                                                                                                          >  2:388575/1‑141 (MQ=255)
                   tACAATCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGCTATTTCGATGTACAATGACAGACAGgtttaagtttaa                                                                                                                          >  1:470615/1‑141 (MQ=14)
                    acactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaa                                                                                                                         >  2:660218/5‑141 (MQ=255)
                    acacTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGCTGTACAATGGCAGTCAGGTTTAAGTTTaaa                                                                                                                         >  1:215146/1‑141 (MQ=255)
                         cAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATGCCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATGTCAATGTATAATGGCAGTAAGGTTTAAGTTTAAAAGCtt                                                                                                                    >  1:346271/1‑141 (MQ=14)
                           aTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTa                                                                                                                  >  2:93876/1‑141 (MQ=255)
                            tACCTTTTAAAGTATTCTTTGCTGTATTGAATCGTTGGTACCTTGTCTTTCTTACTTTACTATGACGGTGATCTTGCTCAATGAGGTTCTTCCGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTaa                                                                                                                 >  1:323679/1‑141 (MQ=14)
                             aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAt                                                                                                                <  1:547318/141‑1 (MQ=255)
                             aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAt                                                                                                                >  1:508812/1‑141 (MQ=255)
                               cTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTAAGATATTTCGATGTACAATGGAAGTCAGGTTAAAGTTTAAAAGCATTAAtaa                                                                                                              >  2:596894/1‑139 (MQ=14)
                                  ttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGGGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTaattaatt                                                                                                           >  2:12686/1‑141 (MQ=255)
                                  ttAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTattt                                                                                                           <  2:380101/141‑4 (MQ=255)
                                       gTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTaacc                                                                                                      <  1:595871/141‑4 (MQ=255)
                                        tATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAGGTTTAAAAGCTTTAATTACTTTAACCa                                                                                                     >  1:55593/1‑141 (MQ=255)
                                        tATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCa                                                                                                     >  2:581605/1‑141 (MQ=255)
                                         aTTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAt                                                                                                    >  1:247179/1‑141 (MQ=255)
                                           tatttaacgggcggATACGTTGATCTCCTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTg                                                                                                  <  1:651667/128‑1 (MQ=14)
                                           tCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTg                                                                                                  <  1:149277/141‑1 (MQ=39)
                                                   gTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGCTCTTGCTCAATGCGGTTATTCAGATATTTCGATGTACAATGGCCGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTCCCTTc                                                                                          >  2:297314/1‑141 (MQ=14)
                                                   gTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAAGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTc                                                                                          >  1:411092/1‑141 (MQ=255)
                                                     aTTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGt                                                                                        <  2:634991/141‑1 (MQ=255)
                                                        caTACTTTGACACCTTGTCTTCCTCACTTTAATATGACGGTGATCTTGCCCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGa                                                                                     <  2:166184/140‑1 (MQ=14)
                                                         acaCTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTTATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCTGGTTTAAGTTTAAAAGCTTTACTTACTTTAACCATTGCTACCTTCGTTGaa                                                                                    <  2:117765/139‑1 (MQ=14)
                                                           aCTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAgg                                                                                  >  1:228108/1‑141 (MQ=255)
                                                            cTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGt                                                                                 <  1:571510/141‑1 (MQ=255)
                                                            cTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGt                                                                                 >  1:235783/1‑141 (MQ=39)
                                                             tttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTATAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTg                                                                                <  1:458950/141‑1 (MQ=255)
                                                                gATATCTTGTCTTTCTTACTTTAATATGACGGTGATGTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCt                                                                             <  1:370706/141‑1 (MQ=255)
                                                                 aTACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGACTg                                                                            >  2:448586/1‑141 (MQ=38)
                                                                  tatcTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTg                                                                                >  1:197616/4‑136 (MQ=255)
                                                                   atcTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTgtt                                                                          >  1:313479/3‑139 (MQ=255)
                                                                     cTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTTCTTTAACCATTGCAACCTTCGTTGAAGGTGCCTGATCt                                                                        <  1:428516/141‑1 (MQ=255)
                                                                      ttGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTg                                                                       >  2:28104/1‑141 (MQ=255)
                                                                        ttctttcttACTTTAATATGACTGTGATCTTGCTCAATGATGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAATCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTa                                                                     <  2:413937/140‑1 (MQ=11)
                                                                         tctttcttACTTTAATCTGGCCATGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTaa                                                                    <  2:136305/141‑1 (MQ=11)
                                                                            ttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTAAAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTATCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTa                                                                 >  2:321692/1‑141 (MQ=14)
                                                                              cttCCTTTAATATGACGGTGATCCTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAcc                                                               <  1:117847/141‑1 (MQ=255)
                                                                              cttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCCTTGCTACCTTCGCTGAAGGTGCCTGATCTGTTATTAcc                                                               >  2:537536/1‑141 (MQ=255)
                                                                              catACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATGTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAcc                                                               >  2:577494/3‑141 (MQ=255)
                                                                                 tcTTTAATATGACGGTGATCTTGCTCAATGGGGGTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCttt                                                            <  2:435626/140‑1 (MQ=255)
                                                                                  cTTGAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTTATTACCtttt                                                           >  1:263551/1‑141 (MQ=25)
                                                                                   tttAATAAGACGGTGATCTAGCTTAATGAAGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTg                                                          <  2:247987/141‑1 (MQ=11)
                                                                                     tAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTgcg                                                        >  2:27030/1‑139 (MQ=255)
                                                                                       atatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAggc                                                      <  1:640054/141‑2 (MQ=255)
                                                                                       atatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTCCGTCGTAGGAGCCTGATCTGTAACTACCTTTTGAggc                                                      <  1:660815/141‑2 (MQ=11)
                                                                                           gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTACGTACCTTTTGAGACTTAc                                                  >  2:613034/1‑141 (MQ=11)
                                                                                           gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                  <  1:69154/141‑1 (MQ=34)
                                                                                            aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                 >  1:84159/1‑141 (MQ=21)
                                                                                            aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                 >  2:193501/1‑141 (MQ=255)
                                                                                               gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGCTTACCaaa                                              >  1:564064/1‑141 (MQ=12)
                                                                                               gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                              <  2:585560/141‑1 (MQ=32)
                                                                                                tGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAt                                             >  2:340107/1‑141 (MQ=17)
                                                                                                   tCTTGCTCAATGAGGTTATTCGGAACTGTCGGTGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAGCAAATTGt                                          >  2:643674/1‑141 (MQ=11)
                                                                                                   tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCATTTGAGGTTTACCAAATTGt                                          >  1:36707/1‑141 (MQ=12)
                                                                                                   tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                          >  2:569943/1‑141 (MQ=32)
                                                                                                     ttGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGttt                                        >  2:311600/1‑141 (MQ=17)
                                                                                                     ttGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGttt                                        >  2:523885/1‑141 (MQ=12)
                                                                                                      tgctcgttcgggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTa                                       <  2:199582/131‑1 (MQ=11)
                                                                                                      tGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTACAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTa                                       <  1:384544/141‑1 (MQ=11)
                                                                                                       gCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTaa                                      >  1:498680/1‑141 (MQ=32)
                                                                                                       gCTCAATGAGGTTATTCAAATATTTGCATGGACAAGGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTaa                                      <  1:619131/141‑1 (MQ=11)
                                                                                                        cTCAATGAGGTTATTCAGAGATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                     >  1:356405/1‑141 (MQ=12)
                                                                                                           aaTGTGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATgag                                  <  2:287281/141‑1 (MQ=11)
                                                                                                              gAGGTTATTCAGATATTTCGATGTACAATGGCAGTCTGGTTTAAGGATAAAAGCTTTAATGAGTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACg                               <  2:69248/141‑1 (MQ=11)
                                                                                                                 gTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                            >  1:602675/1‑141 (MQ=17)
                                                                                                                  ttATTCTGATATGTCGATGTACAATGACAGTCAGGTTGAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                           <  2:361332/141‑1 (MQ=11)
                                                                                                                      tCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaa                       <  2:622169/141‑1 (MQ=12)
                                                                                                                      tCAGATATTTCGATGTACAATGGCAGTCAGGTTGTTGTTTACGCGGGGGCTGGACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaa                       <  1:482658/141‑1 (MQ=11)
                                                                                                                       cAGATATTTCGATGTACAAAGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCGGTTGAGGTTTACCAAATTGTTTAATGAGACGCTTGATaaa                      >  2:476432/1‑141 (MQ=255)
                                                                                                                       cAGATATTTCGATATACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATaaa                      >  2:490478/1‑141 (MQ=11)
                                                                                                                          atatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGc                   >  1:540961/1‑141 (MQ=17)
                                                                                                                          atatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAACTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGc                   >  1:301179/1‑141 (MQ=12)
                                                                                                                           tatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                  <  1:28933/141‑1 (MQ=17)
                                                                                                                           tatTTCGAGGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                  <  2:179861/141‑1 (MQ=12)
                                                                                                                             tttCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCata                >  2:142093/1‑141 (MQ=12)
                                                                                                                              tttgATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCatat               <  1:530245/138‑1 (MQ=12)
                                                                                                                               tCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTTCCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg              <  2:152169/141‑1 (MQ=11)
                                                                                                                                 gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt            <  1:400523/141‑1 (MQ=17)
                                                                                                                                 gATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt            >  1:165367/1‑141 (MQ=32)
                                                                                                                                   tGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGa          >  2:109174/1‑141 (MQ=12)
                                                                                                                                     tACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTCTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAATGCATATGCTGAAt        >  1:450402/1‑141 (MQ=255)
                                                                                                                                      aCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGGCGTTTGATAAACGCATAGTCTGAATg       >  2:546038/1‑141 (MQ=11)
                                                                                                                                      aCAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTCAATg       <  2:462744/141‑1 (MQ=17)
                                                                                                                                        aaTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt     >  2:348740/1‑141 (MQ=14)
                                                                                                                                            |                                                                                                                                           
TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAT  >  CP000730/35947‑36226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: