BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,174	T→C	25.4%	K126K (AAA→AAG)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,174	0	T	C	25.4%	530.5 / 282.2	476	K126K (AAA→AAG)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (165/185); new base C (50/71); total (217/259)
Fisher's exact test for biased strand distribution p-value = 2.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.27e-01

Only 100 of 490 total aligned reads displayed.

ATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAT  >  CP000730/36034‑36314

 tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGtt                                                                                                                                             >  1:446265/1‑141 (MQ=255)

   tGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTa                                                                                                                                           <  1:507716/141‑1 (MQ=35)

    tcggggttacTTGCTCAATGGTGTTATTCTGTTATTTTGATCTACATTGACAGTTAGGGTTAAGTTTAAAAGCTTTAATTATTTTAGCCACTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAc                                                                                                                                          <  2:549027/132‑1 (MQ=11)

    gACGGTGATCTTGGTCAAGGAGTTTATTCAGATATTTCGATGGACAAGGACAGTTAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCATTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAc                                                                                                                                          <  2:275585/141‑1 (MQ=11)

    gACGGTGATCTTGCTCAATGATGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                                                                                                          <  1:85568/141‑1 (MQ=255)

    gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                                                                                                          <  1:69154/141‑1 (MQ=34)

    gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                                                                                                          <  1:568524/141‑1 (MQ=35)

     aaagTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAATAGCTTTCATTACTTTACCCATTGCTACATTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                                         >  2:130072/4‑141 (MQ=11)

     aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                                         >  2:537363/1‑141 (MQ=25)

     aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                                         >  1:325658/1‑141 (MQ=21)

     aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                                         >  2:193501/1‑141 (MQ=255)

     aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                                         >  2:28933/1‑141 (MQ=21)

        gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                                      <  2:585560/141‑1 (MQ=32)

         tGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAt                                                                                                                                     >  2:340107/1‑141 (MQ=17)

         tGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCCTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAt                                                                                                                                     <  1:557856/141‑1 (MQ=12)

           aTCTTGCTCAATGAGGGTATGCGTATATTTCGATGTACTATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTg                                                                                                                                   <  2:103932/141‑1 (MQ=11)

            tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                                  >  1:276590/1‑141 (MQ=17)

            tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                                  >  2:579720/1‑141 (MQ=255)

            tCTTACTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTACTTACCTCTGCAGATTTACCAAATTGt                                                                                                                                  >  2:112103/1‑141 (MQ=11)

               tgctcgttcgggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTa                                                                                                                               <  2:199582/131‑1 (MQ=11)

                 cTCAATGAGGTTATTCAGAGATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                                                                                                             >  1:356405/1‑141 (MQ=12)

                    aaTGAGGTTATTCAAATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgag                                                                                                                          <  2:29749/141‑1 (MQ=12)

                     aTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTCACCAGTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgaga                                                                                                                         <  1:256542/141‑1 (MQ=11)

                      tGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGAc                                                                                                                        <  1:458130/141‑1 (MQ=11)

                       gAGGTTATTCAGATATTTCGATGTACAATGGCAGTCTGGTTTAAGGATAAAAGCTTTAATGAGTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACg                                                                                                                       <  2:69248/141‑1 (MQ=11)

                        gggTTATTCAGATATTTTGATGCACAATGTCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                                      <  2:520849/140‑1 (MQ=255)

                        aGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGt                                                                                                                      <  2:478841/141‑1 (MQ=11)

                         ggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                                                                                                                     <  1:125682/141‑1 (MQ=17)

                          gTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                                                                                                                    >  1:636335/1‑141 (MQ=12)

                          gTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGttt                                                                                                                    >  2:661603/1‑141 (MQ=255)

                           ttATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTg                                                                                                                   >  1:175517/1‑141 (MQ=17)

                             aTTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGAt                                                                                                                 >  2:406067/1‑141 (MQ=12)

                               tCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaa                                                                                                               <  2:622169/141‑1 (MQ=12)

                               ccAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaa                                                                                                               >  2:31315/2‑141 (MQ=12)

                                cAGATATTTCGATATACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATaaa                                                                                                              >  2:490478/1‑141 (MQ=11)

                                 aGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCACTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAAc                                                                                                             <  2:28392/141‑1 (MQ=11)

                                 aGATAGTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAAc                                                                                                             <  1:134343/141‑1 (MQ=17)

                                  gATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACg                                                                                                            >  2:444342/1‑141 (MQ=12)

                                   atatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGATTACCCTGTTGTTTAATGAGACGTTTCATAAACGc                                                                                                           >  2:331138/1‑141 (MQ=11)

                                   atatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGc                                                                                                           >  1:574863/1‑141 (MQ=11)

                                    tatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                                                          <  1:28933/141‑1 (MQ=17)

                                      tttCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCata                                                                                                        >  2:142093/1‑141 (MQ=12)

                                        tCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg                                                                                                      >  1:230824/1‑141 (MQ=11)

                                           aTGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg                                                                                                   <  2:571986/141‑1 (MQ=12)

                                           aTGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACGTTTTCAGGCTTACCAAATTGTTTAATGAAACGTTTGATAAACGCATATGCTg                                                                                                   >  2:527009/1‑141 (MQ=11)

                                            tGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGa                                                                                                  >  1:521681/1‑141 (MQ=17)

                                            tGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGa                                                                                                  <  1:102195/141‑1 (MQ=32)

                                               gcactGACAGTCAGGTTCAAGCTTAAAAGCTTTACTTATTTCAGCTATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTCATAAACGCATATGCTGAATg                                                                                               <  1:468713/137‑1 (MQ=11)

                                               aCAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTCAATg                                                                                               <  2:462744/141‑1 (MQ=17)

                                                cAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGCCGTTTGATAAACGCATATGCTGACTgt                                                                                              >  2:180450/1‑140 (MQ=255)

                                                cAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGaagaa                                                                                              >  2:23161/1‑138 (MQ=17)

                                                  aTGACAGTCATGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAtt                                                                                            <  1:647560/141‑1 (MQ=17)

                                                  aTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAtt                                                                                            <  1:539893/141‑1 (MQ=32)

                                                   tGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAttt                                                                                           >  1:656752/1‑140 (MQ=255)

                                                    gACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAt                                                                                          <  2:152553/141‑1 (MQ=255)

                                                                                      tttAACCATTGCTACCTTCGTTGAAGTTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                                        <  2:245874/141‑1 (MQ=11)

                                                                                       ttcaccATTGCTACCTTCGTTGAAGGTGCCTAATTTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatgt                                                       <  1:394622/137‑1 (MQ=9)

                                                                                        tAGCCATTGCTGCCTTCGTTTAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTCTGATAAACGCATATGCTGAATGCTTATCTCGTTTCTTACGCAACCAAATATCTAATCTATGTc                                                      <  2:89011/141‑1 (MQ=11)

                                                                                           ccATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATCATTATCTCGTTGCTTCCGCAACCAAATATCTAATGTATGTcccc                                                   >  2:3520/1‑140 (MQ=17)

                                                                                            cATTGCTACCTTCGTTGAAGGTGTCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                                  <  1:244566/141‑1 (MQ=17)

                                                                                              ttGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCCCTg                                                >  2:205126/1‑141 (MQ=11)

                                                                                               tGCTACCTGAGTTGAAGTTGCCTGAGCTTTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAATCAAATATCTAATGTATGTCGCTCTgt                                               <  2:42009/141‑2 (MQ=11)

                                                                                                 cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTAt                                             >  2:121433/1‑141 (MQ=17)

                                                                                                  tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCGGGATc                                            >  2:544211/1‑141 (MQ=17)

                                                                                                  tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACAAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATTTCGTTTCTTACGCAACCAACAATCCAATGTATGTTCCACTGCATc                                            >  2:433642/1‑141 (MQ=11)

                                                                                                  tACCTTCGTTGAAGATGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc                                            >  1:436854/1‑141 (MQ=17)

                                                                                                  tACCTTCGGTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc                                            <  2:660267/141‑1 (MQ=17)

                                                                                                   gccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                           >  1:478424/2‑141 (MQ=255)

                                                                                                   aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATTAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTTCTTACGCAACCAAATATCTCATGTATGTCTCTCTgactca                                           >  1:213094/1‑136 (MQ=11)

                                                                                                   aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                           <  1:588281/141‑1 (MQ=17)

                                                                                                   aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                                           <  2:226601/141‑1 (MQ=32)

                                                                                                    ccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTTCTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                          >  1:625525/1‑141 (MQ=11)

                                                                                                    ccTTCGTTGAAGGTGCCGGCTCTGTAATTACCTTTTGAGGTCTACCAGATTGTTCAATGAGACGTTTGATAAACGCATCTGCTGAATGATGATCTCGTGGCTTACGCAACCAAATATCTAATGTATGTACCTCTGCAGCaa                                          <  2:211052/141‑1 (MQ=11)

                                                                                                     cTTCGTTGAATGTGACTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAAt                                         <  2:360736/141‑1 (MQ=12)

                                                                                                       tCGTTGAAGGTCCCTGATCTGTAATTACCTTTTGATGTTTACCAAATTTTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATgg                                       <  1:197708/141‑1 (MQ=255)

                                                                                                            gAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGa                                  >  1:112321/1‑141 (MQ=32)

                                                                                                              aggtttcTGAACTGTAACTTCCTTTTGATGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGata                                <  1:313558/135‑1 (MQ=11)

                                                                                                               ggTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTAGCAATGGCACGatat                               <  2:454066/141‑1 (MQ=255)

                                                                                                                  gCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCGAAGACACGATATaaa                            >  1:416134/1‑141 (MQ=11)

                                                                                                                    cTGATCTGAAATTACCTTTTTAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATa                          <  2:268784/141‑1 (MQ=12)

                                                                                                                      gATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAGCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAGTGTATGTCCCTCTGCATCGATTGCACGATATAAATAGc                        <  1:373778/141‑1 (MQ=255)

                                                                                                                       aTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCt                       <  2:503960/141‑1 (MQ=32)

                                                                                                                          tGTAATTACCTTTTTATGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCa                    <  2:64111/141‑1 (MQ=12)

                                                                                                                          tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCa                    <  2:250711/141‑1 (MQ=32)

                                                                                                                           gTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAt                   >  1:609394/1‑141 (MQ=12)

                                                                                                                            tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGGTCCAtt                  <  2:369609/141‑1 (MQ=17)

                                                                                                                            tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtt                  <  1:379549/141‑1 (MQ=17)

                                                                                                                            tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCAtt                  >  2:598454/1‑141 (MQ=32)

                                                                                                                            tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAAACAAATATCTAATCTATGTCCCTCTATATCAATGGCACGATATATATAGCTCCAtt                  >  1:514479/1‑141 (MQ=11)

                                                                                                                              aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtttt                >  2:162240/1‑141 (MQ=17)

                                                                                                                               ctACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTc               >  1:298697/2‑141 (MQ=12)

                                                                                                                                tACCTTTTGAGGTTTACCAAATTGTTTAATGAGCCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATTTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTcc              <  2:388073/141‑1 (MQ=17)

                                                                                                                                 aCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCt             <  1:594379/141‑1 (MQ=17)

                                                                                                                                  ccTTTTGAGTCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtt            <  1:351301/141‑1 (MQ=12)

                                                                                                                                    ttttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCCCGATATAAATAGCTCCATTTTCCcttt          >  2:8243/1‑141 (MQ=12)

                                                                                                                                    ttttGAGGCTTACCAAATTGGTTAATGAGACGTTGGAAGAACTCGTATGCTGAAGGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCCCTGTATCAATGGCACGATATAAATAGCTCCATTATCCtttt          >  2:608846/1‑141 (MQ=11)

                                                                                                                                     tttAAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTa         <  1:282688/141‑1 (MQ=17)

                                                                                                                                     cttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCCACGGCACGATATCAATAGCTCCCTTTTCCTTTTa         >  2:412998/2‑141 (MQ=255)

                                                                                                                                        gAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAttt      <  1:545517/141‑1 (MQ=17)

                                                                                                                                         aGGCTTACCAAATTGTTTAATGATACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtttt     <  1:155730/141‑1 (MQ=12)

ATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAT  >  CP000730/36034‑36314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`