Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,574 A→T 4.7% intergenic (‑23/‑500) USA300HOU_0026 ← / → USA300HOU_0028 IS431mec transposase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,5740AT 4.7% 386.0 / 5.1 150intergenic (‑23/‑500)USA300HOU_0026/USA300HOU_0028IS431mec transposase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (72/70);  new base T (4/4);  total (76/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

ACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGT  >  CP000730/36438‑36712
                                                                                                                                        |                                                                                                                                          
acGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATta                                                                                                                                        <  2:293047/141‑1 (MQ=32)
acGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATta                                                                                                                                        <  2:519267/141‑1 (MQ=32)
  gTTCCCTTAATTCTTCAGATATACCACGATACCTTAATGTATATCTTAGCTAGAAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATtata                                                                                                                                      <  1:349663/141‑1 (MQ=11)
     cccTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACATAAGACTCCTTTTTGTTAAAATTATACta                                                                                                                                   <  1:269732/141‑1 (MQ=17)
         tAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATaaa                                                                                                                               <  1:636726/141‑1 (MQ=33)
               ttCAGTTATATCACGATAGCTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAAc                                                                                                                         <  2:53165/141‑1 (MQ=255)
                tCAGATATATCACGATAGCTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGCTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACt                                                                                                                        <  2:596093/141‑1 (MQ=255)
                   gATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTg                                                                                                                     >  1:105887/1‑141 (MQ=35)
                    atatatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGc                                                                                                                    >  1:151888/1‑141 (MQ=35)
                    atatatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGc                                                                                                                    <  2:151888/141‑1 (MQ=35)
                       tatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAAc                                                                                                                 >  1:199032/1‑141 (MQ=35)
                       tatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAAc                                                                                                                 >  1:211530/1‑141 (MQ=35)
                         tCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGGAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAg                                                                                                               <  2:429423/141‑1 (MQ=25)
                            cGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAc                                                                                                            <  2:660108/141‑1 (MQ=35)
                              atagctcgatcgatatCTTAGTGAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTGCATACAGAAGACGCCTTTTNGTTAATATTTTATTATATATTCAACTTTGCAACAGAAcct                                                                                                          <  2:347130/129‑2 (MQ=11)
                              agagctaaaTGCAGATCTTAGAAAGGAGCCCACGGCAACCGCTATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTCCATACAGAAGACGCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCg                                                                                                          <  1:123033/134‑1 (MQ=11)
                              aTAGCTGAATGCATAGCTTATATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAATACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCg                                                                                                          <  2:13521/141‑1 (MQ=11)
                              aTAACTCAATGACTAGCTTAGATAGTAGCCAACAGCTACAGTGATAACATTCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGGCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcct                                                                                                          <  2:436827/141‑2 (MQ=11)
                               taggtCAATGTATATATTAGACAGTAGCCAACGGCTCCAGTGCTAAAATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctc                                                                                                         <  1:302909/137‑3 (MQ=11)
                               tagcgcAATGCATATCTTAGATAGTAGCCAACGGCTACAGTTATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                         <  2:316804/136‑1 (MQ=255)
                               tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                         <  1:72600/138‑1 (MQ=12)
                               cagcttaaTGCATATATTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTTCTTTTTGTTCAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                         <  1:24872/135‑1 (MQ=11)
                               aagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAATCGt                                                                                                         <  1:480658/138‑1 (MQ=255)
                               aagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTATTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                         <  1:399491/138‑1 (MQ=255)
                                agcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTATATTTATATTATAAATACAACTTTTCAACATAACCGta                                                                                                        >  2:170807/3‑141 (MQ=11)
                                aaCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGta                                                                                                        >  2:631284/1‑141 (MQ=38)
                                aaCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGta                                                                                                        <  2:185170/141‑1 (MQ=37)
                                 gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACGTCCTTGTTAAATTGTTTATATCTGAAATAGGTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAAAACCGtat                                                                                                       >  1:155309/2‑141 (MQ=11)
                                 gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                       >  1:222228/2‑141 (MQ=12)
                                 gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                       >  1:306638/2‑141 (MQ=12)
                                  cTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAATTTATACTATAAATTCAACTTTGCAACAGAACCGtatt                                                                                                      >  2:567772/1‑141 (MQ=255)
                                  cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCACCAGAACCGtatt                                                                                                      >  1:125607/1‑141 (MQ=12)
                                  cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATAGTTTATATCTGCAATAGTTCATACAGACGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGATCCGtatt                                                                                                      >  2:74901/1‑141 (MQ=11)
                                     aaTGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATg                                                                                                   >  2:245572/1‑141 (MQ=40)
                                      atgcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttat                                                                                                  <  2:137032/137‑5 (MQ=255)
                                      aTGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTCATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAGTATGCTATAAGTTCAACTTTTCACCAGAACCGTATTATgg                                                                                                  >  2:23050/1‑141 (MQ=14)
                                       tgcttatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                 <  1:547354/137‑6 (MQ=255)
                                       gtcatatCTTAGATAGTAGCCAACGGCTACAGGGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgt                                                                                                 <  1:620980/138‑11 (MQ=11)
                                       gggatacCTTGGATAGTAGCCAACAGCTACAGGGATAACATCCTGGTTAAATTGTTTATATCTGAAATGGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttacta                                                                                                 <  2:218062/138‑11 (MQ=11)
                                       cgcatgtCTTAGATGGTAGCCGACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAGTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                 <  1:561582/135‑6 (MQ=11)
                                        gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatga                                                                                                <  2:581934/139‑7 (MQ=255)
                                        gcatatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactat                                                                                                <  1:623996/139‑12 (MQ=11)
                                        gcatatCTTAGATAGTAGCCAACAGCTACAGTGACAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGATTCCTTTTTGTTAATATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactat                                                                                                <  1:405534/139‑12 (MQ=11)
                                         tataTCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAAt                                                                                               <  1:37897/141‑1 (MQ=255)
                                         catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTCAATTGTTTATATCTGAGATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAGCAGAACTGtattttatgaa                                                                                               >  2:27103/2‑134 (MQ=11)
                                         catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgat                                                                                               >  1:117612/2‑134 (MQ=255)
                                          atatCTTAGATATTAGCCAACGGTTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATa                                                                                              <  2:548214/141‑1 (MQ=255)
                                          atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatt                                                                                              >  1:550434/1‑128 (MQ=11)
                                          atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatt                                                                                              >  1:238331/1‑128 (MQ=11)
                                          atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGTACCGTATTATGGAATa                                                                                              <  1:398122/141‑1 (MQ=255)
                                          atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTGTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTAGATTATAAATTCAACTTTGCAACAGAAcctcctaacggtatt                                                                                              >  2:523521/1‑128 (MQ=11)
                                           tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatta                                                                                             >  2:517247/1‑127 (MQ=11)
                                           tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatatg                                                                                             <  2:50853/141‑15 (MQ=11)
                                           tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatatg                                                                                             >  1:184231/1‑127 (MQ=11)
                                            atCTTAGATAGTAGCCAACGGCTACCGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtac                                                                                            <  1:25144/141‑11 (MQ=11)
                                            atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATaga                                                                                            <  1:101632/141‑1 (MQ=255)
                                                tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTGTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc                                                                                        <  2:92445/141‑15 (MQ=11)
                                                 gaggagtggCCAATGGCTTCGGTGATAACATCCTTTTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGt                                                                                       <  2:293886/137‑1 (MQ=17)
                                                      gtagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTa                                                                                  <  1:258182/141‑1 (MQ=255)
                                                       tagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACGAAACCGTATTATGGAATAGAGATGTTGATaa                                                                                 >  2:70012/1‑141 (MQ=255)
                                                        agCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAAc                                                                                >  1:38468/1‑141 (MQ=255)
                                                           cAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAAAGATGTTGGTAACAtt                                                                             >  1:416867/1‑141 (MQ=255)
                                                             aCGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTta                                                                           <  1:565821/141‑1 (MQ=255)
                                                               ggCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATGATTTCAACGTTGCAACGTAACGGTGGTATGGGGTAGATATGGTGGTAACagtaagg                                                                         >  1:138467/1‑135 (MQ=11)
                                                               ggCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTtgta                                                                         >  2:104980/1‑141 (MQ=255)
                                                               ggCTACAGTGATAACATCCTTGTTAAATTGTTTATATATGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTtata                                                                         >  2:659180/1‑141 (MQ=255)
                                                               ggCCACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTtata                                                                         <  2:447601/141‑1 (MQ=255)
                                                                     aGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGAt                                                                   >  1:203910/1‑141 (MQ=255)
                                                                      gTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATc                                                                  <  1:436815/141‑1 (MQ=255)
                                                                                ccTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTa                                                        >  2:354674/1‑141 (MQ=255)
                                                                                    gTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGtt                                                    <  1:639338/141‑1 (MQ=255)
                                                                                         aTTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAAtt                                               >  2:157050/1‑141 (MQ=255)
                                                                                           tGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTTTTATTTTACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAGCATTTATACAGGATCATTATACTTAAGTTTAATTTc                                             >  2:477669/1‑141 (MQ=25)
                                                                                               tataTCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGtta                                         <  1:127539/141‑1 (MQ=255)
                                                                                               tataTCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGtta                                         >  2:179134/1‑141 (MQ=255)
                                                                                               tataTCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGtta                                         <  1:49888/141‑1 (MQ=255)
                                                                                                 tatCTGAAATAGTTCATACAGAAGACTCCTTTGTGGTAAAATTATACTATAAATTTGAGTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGttatt                                       >  1:455541/1‑141 (MQ=37)
                                                                                                      gAAATAGTTCATACAGAAGACACCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATCGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGa                                  >  1:345919/1‑141 (MQ=255)
                                                                                                       aaaTAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGaa                                 <  1:58399/141‑1 (MQ=255)
                                                                                                         acaGTTCATACATACGACTCCTTTTTGTTAAACTTATACTATAAATTCAACCTTGCAACAGAACCGTATTATGGAATAGAGATGTTGTAAACATTCATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAAcc                               <  2:608323/139‑1 (MQ=255)
                                                                                                         aTAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAAcc                               >  1:568912/1‑141 (MQ=255)
                                                                                                         aTAGTTCATACAGAAGACCCCTTTTTGTTAATATTATACTATTAACTCAACTTTGCAACAGAACCGTATTATGGAATAGAGCTGTTGGTAACATTTATACAGGATCATTATTCTTAAGTTCGATTTCGTTATTACAGAAcc                               <  2:51926/141‑1 (MQ=255)
                                                                                                          tAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACca                              >  1:71494/1‑141 (MQ=255)
                                                                                                           aGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAGCTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATAATTATACTTAAGATTATTTTCGTTATTACAGAACcac                             >  2:433088/1‑141 (MQ=255)
                                                                                                           aGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcac                             >  2:423636/1‑141 (MQ=255)
                                                                                                            gTTCATACAGAAGACTCCTTTTTTTTTTTTTTTTACTATAAATTCAACTTTGCAACAGATCCGTTTTATGGAATAGATCAGTTGGTAGCATTTCTACAGTTTCATTATACTTAAGTTTAATTTCGttattttataacttca                            >  2:112467/1‑130 (MQ=19)
                                                                                                            gTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcaca                            <  2:211530/141‑1 (MQ=255)
                                                                                                            gTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTAAAAATTGGGACACAACAGACTGCGCGAGTAGCGTTGTTGGTAACATTTATACAGGATCATCATACTTAAGTTTAATTTCGTTATTACAGAACcaca                            >  1:488661/1‑141 (MQ=21)
                                                                                                             ttCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTGTGCAGGTTCATTATACTTAAGTTTAATTTCGTTATTACAGAACcacac                           >  1:55333/1‑141 (MQ=255)
                                                                                                                    agaagaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATAATTAAGTTTAATTTCGTTATTACAGAACCACACATTCCaa                    >  1:418187/1‑141 (MQ=255)
                                                                                                                     gaagaCTCCTTTTTTTTTTTTTTTTACTATAAATTCAACTTTAAAACAGAATCGTATTATGGAGTAGAGATGTTGGTAACTTTTATACAGGATCATTATACATAAGTTTAATTTTTTTTTTATTGATCCACAACTTCCTAc                   >  2:165202/1‑141 (MQ=255)
                                                                                                                      aagaCTCCTTTTTGTTTAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTTTTACTGAACCACACATTCAAAcc                  >  2:239196/1‑141 (MQ=255)
                                                                                                                       agaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCa                 <  1:484384/141‑1 (MQ=255)
                                                                                                                         aCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCATATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCaga               >  1:557435/1‑141 (MQ=255)
                                                                                                                            ccTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCACCCagaagg            >  2:23305/1‑140 (MQ=255)
                                                                                                                            ccTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCagaaga            >  1:113443/1‑141 (MQ=255)
                                                                                                                             cTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAagag           >  2:344306/1‑141 (MQ=255)
                                                                                                                               ttttGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGaa         <  1:64662/141‑1 (MQ=255)
                                                                                                                                    ttAAAATTGTACTCTAAATTCAACATTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCGTTATACTTAAGATTAATTTCGTTATTACAGAACCACACATGACAACAAGAAGAGAAAGtct    >  1:426540/1‑139 (MQ=255)
                                                                                                                                     tAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTATTTTCGTTATTACAGAACTACACATTCCAACCAGAAGAGAAAGTTTg   >  2:246603/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                          
ACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGT  >  CP000730/36438‑36712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: