Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,581 C→T 29.1% intergenic (‑30/‑493) USA300HOU_0026 ← / → USA300HOU_0028 IS431mec transposase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,5810CT29.1% 223.1 / 96.8 150intergenic (‑30/‑493)USA300HOU_0026/USA300HOU_0028IS431mec transposase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (56/50);  new base T (21/23);  total (77/73)
Fisher's exact test for biased strand distribution p-value = 5.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

CCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTTAG  >  CP000730/36443‑36720
                                                                                                                                          |                                                                                                                                           
cccTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACATAAGACTCCTTTTTGTTAAAATTATACta                                                                                                                                           <  1:269732/141‑1 (MQ=17)
    tAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATaaa                                                                                                                                       <  1:636726/141‑1 (MQ=33)
              gATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTg                                                                                                                             >  1:105887/1‑141 (MQ=35)
               atatatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGc                                                                                                                            >  1:151888/1‑141 (MQ=35)
               atatatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGc                                                                                                                            <  2:151888/141‑1 (MQ=35)
                aatatCACGATAGCTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTTATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCa                                                                                                                           <  2:442934/140‑1 (MQ=255)
                  tatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAAc                                                                                                                         >  1:211530/1‑141 (MQ=35)
                  tatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAAc                                                                                                                         >  1:199032/1‑141 (MQ=35)
                    tCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGGAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAg                                                                                                                       <  2:429423/141‑1 (MQ=25)
                       cGATAACTTAATGTATATCTTATATATGAGCCAACGGTTACAGTGATAACATCATTGTTAAATTGTTTATATCTGAAATAGTTCAAACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAc                                                                                                                    <  2:31324/141‑1 (MQ=11)
                         agagctaaaTGCAGATCTTAGAAAGGAGCCCACGGCAACCGCTATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTCCATACAGAAGACGCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCg                                                                                                                  <  1:123033/134‑1 (MQ=11)
                         aTAGCTGAATGCATAGCTTATATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAATACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCg                                                                                                                  <  2:13521/141‑1 (MQ=11)
                         aTAACTCAATGACTAGCTTAGATAGTAGCCAACAGCTACAGTGATAACATTCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGGCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcct                                                                                                                  <  2:436827/141‑2 (MQ=11)
                          taggtCAATGTATATATTAGACAGTAGCCAACGGCTCCAGTGCTAAAATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctc                                                                                                                 <  1:302909/137‑3 (MQ=11)
                          tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                                 <  1:72600/138‑1 (MQ=12)
                          cagcttaaTGCATATATTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTTCTTTTTGTTCAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                                 <  1:24872/135‑1 (MQ=11)
                          aagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTATTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                                 <  1:399491/138‑1 (MQ=255)
                           agctaaaTGCATATATTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTTAAATTATATTATAAATTCAACTTTGCAACATAACCGta                                                                                                                >  2:637716/6‑141 (MQ=11)
                           agcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTATATTTATATTATAAATACAACTTTTCAACATAACCGta                                                                                                                >  2:170807/3‑141 (MQ=11)
                           aaCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGta                                                                                                                <  2:185170/141‑1 (MQ=37)
                            gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACGTCCTTGTTAAATTGTTTATATCTGAAATAGGTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAAAACCGtat                                                                                                               >  1:155309/2‑141 (MQ=11)
                            gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                               >  1:306638/2‑141 (MQ=12)
                            gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                               >  1:222228/2‑141 (MQ=12)
                            gcTCAATCCATATCTTAGATGGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTAAACTTAGCAACAGAACCGtat                                                                                                               >  1:417661/2‑141 (MQ=11)
                             cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCACCAGAACCGtatt                                                                                                              >  1:125607/1‑141 (MQ=12)
                                aaTGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATg                                                                                                           >  2:245572/1‑141 (MQ=40)
                                 atgcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttat                                                                                                          <  2:137032/137‑5 (MQ=255)
                                 aTGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTCATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAGTATGCTATAAGTTCAACTTTTCACCAGAACCGTATTATgg                                                                                                          >  2:23050/1‑141 (MQ=14)
                                  gtcatatCTTAGATAGTAGCCAACGGCTACAGGGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgt                                                                                                         <  1:620980/138‑11 (MQ=11)
                                  gggatacCTTGGATAGTAGCCAACAGCTACAGGGATAACATCCTGGTTAAATTGTTTATATCTGAAATGGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttacta                                                                                                         <  2:218062/138‑11 (MQ=11)
                                  cgcatgtCTTAGATGGTAGCCGACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAGTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                         <  1:561582/135‑6 (MQ=11)
                                   gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatga                                                                                                        <  2:581934/139‑7 (MQ=255)
                                   gcatatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactat                                                                                                        <  1:623996/139‑12 (MQ=11)
                                    catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTCAATTGTTTATATCTGAGATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAGCAGAACTGtattttatgaa                                                                                                       >  2:27103/2‑134 (MQ=11)
                                    catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgat                                                                                                       >  1:117612/2‑134 (MQ=255)
                                     atatCTTAGATATTAGCCAACGGTTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATa                                                                                                      <  2:548214/141‑1 (MQ=255)
                                     atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatt                                                                                                      >  1:550434/1‑128 (MQ=11)
                                     atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatt                                                                                                      >  1:238331/1‑128 (MQ=11)
                                     atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTGTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTAGATTATAAATTCAACTTTGCAACAGAAcctcctaacggtatt                                                                                                      >  2:523521/1‑128 (MQ=11)
                                     atatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatat                                                                                                      <  1:91702/141‑14 (MQ=11)
                                      tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtattt                                                                                                     >  1:429631/1‑127 (MQ=11)
                                      tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatta                                                                                                     >  2:517247/1‑127 (MQ=11)
                                      tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatatg                                                                                                     >  1:184231/1‑127 (MQ=11)
                                      tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatatg                                                                                                     <  2:344940/141‑15 (MQ=11)
                                       atCTTAGATAGTAGCCAACGGCTACCGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtac                                                                                                    <  1:25144/141‑11 (MQ=11)
                                       atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATaga                                                                                                    <  1:101632/141‑1 (MQ=255)
                                          ttAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacact                                                                                                 >  2:521862/1‑128 (MQ=11)
                                           tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTGTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc                                                                                                <  2:92445/141‑15 (MQ=11)
                                            gaggagtggCCAATGGCTTCGGTGATAACATCCTTTTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGt                                                                                               <  2:293886/137‑1 (MQ=17)
                                            aGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGt                                                                                               >  1:475240/1‑141 (MQ=255)
                                                 gtagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTa                                                                                          <  1:258182/141‑1 (MQ=255)
                                                  tagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACGAAACCGTATTATGGAATAGAGATGTTGATaa                                                                                         >  2:70012/1‑141 (MQ=255)
                                                   agCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTTTTTTTTTACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAAc                                                                                        >  2:547997/1‑141 (MQ=17)
                                                   agCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAAc                                                                                        >  2:49888/1‑141 (MQ=255)
                                                   agCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAAc                                                                                        >  1:38468/1‑141 (MQ=255)
                                                      cAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAAAGATGTTGGTAACAtt                                                                                     >  1:416867/1‑141 (MQ=255)
                                                        aCGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTta                                                                                   <  1:565821/141‑1 (MQ=255)
                                                          ggCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATGATTTCAACGTTGCAACGTAACGGTGGTATGGGGTAGATATGGTGGTAACagtaagg                                                                                 >  1:138467/1‑135 (MQ=11)
                                                          ggCTACAGTGATAACATCCTTGTTAAATTGTTTATATATGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTtata                                                                                 >  2:659180/1‑141 (MQ=255)
                                                          ggCCACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTtata                                                                                 <  2:447601/141‑1 (MQ=255)
                                                               cAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTATATTTTTGCTATTAATTCTATTTAGAAGCAGAACGGTAGTCTCGAATATACAAGTTGGTAACGTTtataacgga                                                                            >  2:58186/1‑136 (MQ=9)
                                                               aaggtaTAACATCCTTGTTAAATTGTTTATTTCTGAAATAGTTCATACAGATGTCGCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTAGTATGGAATAGAGATTTTGGTAACATTTATACAGGa                                                                            <  2:77724/136‑1 (MQ=18)
                                                                aGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGAt                                                                           >  1:203910/1‑141 (MQ=255)
                                                                      aaCATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGCTCATtat                                                                     >  1:389997/1‑141 (MQ=255)
                                                                           ccTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTa                                                                >  2:354674/1‑141 (MQ=255)
                                                                              tGTTAAAGTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGt                                                             <  2:397241/141‑1 (MQ=255)
                                                                               gTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGtt                                                            <  1:639338/141‑1 (MQ=255)
                                                                                    aTTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAAtt                                                       >  2:157050/1‑141 (MQ=255)
                                                                                    aTTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAAtt                                                       <  1:516899/141‑1 (MQ=255)
                                                                                         ttATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGtt                                                  >  2:429899/1‑141 (MQ=255)
                                                                                          tataTCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGtta                                                 >  2:179134/1‑141 (MQ=255)
                                                                                          tataTCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGtta                                                 <  1:127539/141‑1 (MQ=255)
                                                                                          tataTCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGtta                                                 <  1:49888/141‑1 (MQ=255)
                                                                                            tatCTGAAATAGTTCATACAGAAGACTCCTTTGTGGTAAAATTATACTATAAATTTGAGTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGttatt                                               >  1:455541/1‑141 (MQ=37)
                                                                                                 gAAATAGTTCATACAGAAGACACCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATCGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGa                                          >  1:345919/1‑141 (MQ=255)
                                                                                                  aaaTAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGaa                                         <  1:58399/141‑1 (MQ=255)
                                                                                                  aaaTAGTTCATACAGAAGACTCCTTTCTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGCTTAATTTCGTTATTACAGaa                                         <  1:404312/141‑1 (MQ=255)
                                                                                                    acaGTTCATACATACGACTCCTTTTTGTTAAACTTATACTATAAATTCAACCTTGCAACAGAACCGTATTATGGAATAGAGATGTTGTAAACATTCATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAAcc                                       <  2:608323/139‑1 (MQ=255)
                                                                                                    aTAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAAcc                                       >  1:568912/1‑141 (MQ=255)
                                                                                                      aGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcac                                     <  1:354674/141‑1 (MQ=255)
                                                                                                       gTTCATACAGAAGACTCCTTTTTTTTTTTTTTTTACTATAAATTCAACTTTGCAACAGATCCGTTTTATGGAATAGATCAGTTGGTAGCATTTCTACAGTTTCATTATACTTAAGTTTAATTTCGttattttataacttca                                    >  2:112467/1‑130 (MQ=19)
                                                                                                       gTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcaca                                    <  2:211530/141‑1 (MQ=255)
                                                                                                        ttCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTGTGCAGGTTCATTATACTTAAGTTTAATTTCGTTATTACAGAACcacac                                   >  1:55333/1‑141 (MQ=255)
                                                                                                        ttCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcactg                                   >  2:666356/1‑139 (MQ=255)
                                                                                                                gaagaCTCCTTTTTTTTTTTTTTTTACTATAAATTCAACTTTAAAACAGAATCGTATTATGGAGTAGAGATGTTGGTAACTTTTATACAGGATCATTATACATAAGTTTAATTTTTTTTTTATTGATCCACAACTTCCTAc                           >  2:165202/1‑141 (MQ=255)
                                                                                                                gaagaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAAc                           >  1:667825/1‑141 (MQ=255)
                                                                                                                 aagaCTCCTTTTTGTTTAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTTTTACTGAACCACACATTCAAAcc                          >  2:239196/1‑141 (MQ=255)
                                                                                                                  agaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCa                         <  1:484384/141‑1 (MQ=255)
                                                                                                                    aCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCATATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCaga                       >  1:557435/1‑141 (MQ=255)
                                                                                                                       ccTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCACCCagaagg                    >  2:23305/1‑140 (MQ=255)
                                                                                                                       ccTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCagaaga                    >  1:113443/1‑141 (MQ=255)
                                                                                                                        cTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAagag                   >  2:344306/1‑141 (MQ=255)
                                                                                                                          ttttGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGaa                 <  1:64662/141‑1 (MQ=255)
                                                                                                                          tttgGTTAAAAATAAACTATAAATTCAACATTGCAACAGAAGCGTATGATGGAATATAGATGTTGGTAACATTTATACAGGATCATTAGACTTAAGTTTAATTTCCTTATTACAGAACCACTCCTTCCAACCataagttag                 <  2:60666/141‑5 (MQ=255)
                                                                                                                              gTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATCGCGGTGTTGGTCCCATTTATACAGGATCATTATACTGCTGTTTAGTTTCGGTATTACAGAACCACGCATTCCAAACAGAAGAGAAAgga             >  2:517734/1‑139 (MQ=37)
                                                                                                                               ttAAAATTGTACTCTAAATTCAACATTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCGTTATACTTAAGATTAATTTCGTTATTACAGAACCACACATGACAACAAGAAGAGAAAGtct            >  1:426540/1‑139 (MQ=255)
                                                                                                                                tAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTATTTTCGTTATTACAGAACTACACATTCCAACCAGAAGAGAAAGTTTg           >  2:246603/1‑141 (MQ=255)
                                                                                                                                tAAAATTACACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATg           <  1:48701/141‑1 (MQ=255)
                                                                                                                                      tataCTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTAtt     <  2:110000/141‑1 (MQ=255)
                                                                                                                                         aCTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTTAg  <  1:547997/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTTAG  >  CP000730/36443‑36720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: