Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,180 C→T 27.7% intergenic (+238/‑896) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,1800CT27.7% 192.9 / 59.6 95intergenic (+238/‑896)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (40/28);  new base T (12/14);  total (53/42)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

TGAATTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATT  >  CP000730/76040‑76318
                                                                                                                                            |                                                                                                                                          
tGAATTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATAAAGGTTCTGTTGCAAAGTTGAATc                                                                                                                                            >  1:39227/1‑141 (MQ=255)
       aaTATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGta                                                                                                                                     <  2:284609/141‑1 (MQ=255)
       aaTATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGAGTTAGTAATCCAGGTACTACTAACACAAAGGCAATTAATTTAAAAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGta                                                                                                                                     <  2:194544/141‑1 (MQ=255)
          ataACTAATATTGCTGCACCTGTATCACTTCGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTACTAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGACTCTATAGTATaa                                                                                                                                  <  2:491584/141‑1 (MQ=255)
            aaCTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAtt                                                                                                                                >  1:602122/1‑141 (MQ=255)
                  tatTGCTGCACCTGTATCACTTAGAAAAGCTGCCCAAGGTGATAGAGAGCGAGGGAATACTGAGACGAAGGGGAGTGATGTAAGAATTAGAGCAACTGTTAGGGTCGGTTGCACAGGTGAATCTACAGGAAACttttgaaa                                                                                                                          >  2:70113/1‑137 (MQ=255)
                    ttGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATGATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTaaaaaa                                                                                                                        >  2:254338/1‑141 (MQ=255)
                          cACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTACAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAg                                                                                                                  >  1:81576/1‑141 (MQ=255)
                           aCCTGTTTCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACGAACACATAGGCAATTAATTTAATAATTATAGCATATTATTGGTTTTGTTGCAAAGGTGAATCTATAGTATAATTTTAACAAAAAGGAGt                                                                                                                 <  2:441429/141‑1 (MQ=38)
                            ccTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtc                                                                                                                >  2:92884/1‑141 (MQ=255)
                              tGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtctt                                                                                                              <  1:504653/141‑1 (MQ=255)
                               gTATCACTTAGAACAGGTAACGAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAGTTTTAACAAAAAGAAGgcttc                                                                                                             >  1:343857/1‑141 (MQ=38)
                                    aCTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTAt                                                                                                        >  1:496913/1‑141 (MQ=255)
                                    aCTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGCTTTCAGTAt                                                                                                        >  2:31412/1‑141 (MQ=255)
                                       tAGGACATCTAACCAAAGTGTTAGTAGTCCAGGGAATACGAACACAAAGGCAATTAATTTAATAATTATAGCGAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCGTCTGTATGaa                                                                                                     <  2:313260/141‑1 (MQ=37)
                                        aGAACAGCTAACCAAAGTGTTACGAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAAc                                                                                                    <  1:87189/141‑1 (MQ=255)
                                         gAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACt                                                                                                   >  1:598675/1‑141 (MQ=255)
                                           aCAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAt                                                                                                 >  2:545556/1‑141 (MQ=255)
                                            cAGCTAACTAAAGTGTTAGTAATCCAGGGAATACTACCACAAAGCCAATTAATTTAATAATTATAGCAAAATATAGGTTGTGTTGCAAAGTTGAATCGATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAtt                                                                                                <  2:57972/141‑1 (MQ=37)
                                            cAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAAGACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGGTGCAAAGTTGAATCTATAGAATAATTTTAACAAAACTGAGTCTTCTGAATGAACGAtt                                                                                                >  2:498507/1‑141 (MQ=255)
                                            cAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAtt                                                                                                >  1:559443/1‑141 (MQ=255)
                                              gCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAtttg                                                                                              >  1:4824/1‑140 (MQ=255)
                                              gCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTc                                                                                              <  2:552835/141‑1 (MQ=255)
                                                 aaCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGa                                                                                           >  2:511566/1‑141 (MQ=255)
                                                 aaCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGa                                                                                           <  1:282016/141‑1 (MQ=255)
                                                  aCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAACAAGGAGTCTTCTGTATGAACTATTTCAGat                                                                                          >  1:255164/1‑141 (MQ=255)
                                                  aCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAgga                                                                                          >  1:210876/1‑139 (MQ=255)
                                                  aCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGATCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAGAAAGGAGTCTTCTGTATGAACTATTTCAGat                                                                                          >  1:108027/1‑141 (MQ=255)
                                                      aaGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATaa                                                                                      <  1:581292/141‑1 (MQ=255)
                                                       aGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATaaa                                                                                     <  1:259586/141‑1 (MQ=255)
                                                         tgtTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACa                                                                                   <  2:473022/141‑1 (MQ=255)
                                                            tAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAATAGTTGTCTGCTGTATGAACTATTTCAGATATAAACAAtt                                                                                >  1:622334/1‑141 (MQ=255)
                                                            tAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGCGTCTTCCGTATGAACTATTTCAGATATAAACAAtt                                                                                >  1:618928/1‑141 (MQ=255)
                                                             aGTAATCCAGGGAATACTAACACAAATGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAttt                                                                               <  1:508452/141‑1 (MQ=255)
                                                             aGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCAGTTGCAAAGATGAATCTGTAGTATAATGTTAACAAAAAGGAGGCTTCTGTATGAACTATTTCAGATATAAACAAttt                                                                               >  2:222742/1‑141 (MQ=25)
                                                                 aTCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACa                                                                           >  2:8503/1‑141 (MQ=255)
                                                                   ccAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAg                                                                         >  1:542632/1‑141 (MQ=255)
                                                                    cAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATg                                                                                                       >  1:632330/1‑110 (MQ=255)
                                                                    cAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATg                                                                                                       <  2:632330/110‑1 (MQ=255)
                                                                      gggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGAt                                                                      >  1:184015/1‑141 (MQ=255)
                                                                       ggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATg                                                                     <  1:38296/141‑1 (MQ=255)
                                                                       ggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATg                                                                     >  1:353690/1‑141 (MQ=255)
                                                                       ggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATg                                                                     >  1:426699/1‑141 (MQ=255)
                                                                        gAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGt                                                                    >  1:666831/1‑141 (MQ=255)
                                                                        gAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGt                                                                    <  1:614740/141‑1 (MQ=255)
                                                                         aaaaCTAACAAAAAGGGAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGtt                                                                   <  2:333569/138‑1 (MQ=255)
                                                                             cTAACACAAAGGCAATTAATTTAATAATTCTAGAAAAATATAGGACCTGATGCAAAGTTGGATCTATAGTATTAGTTTAATAATAAGGAGTCTTCGGTATGTACTATTTCAGATATAAAGAATTTAATAAGGATGTTATCa                                                               >  2:131571/1‑141 (MQ=255)
                                                                               aaCACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACt                                                             <  2:85143/141‑1 (MQ=255)
                                                                                acacAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTg                                                            <  2:396282/141‑1 (MQ=255)
                                                                                     aaGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                       <  2:553893/141‑1 (MQ=255)
                                                                                            ttaatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGct                                                >  2:27230/1‑141 (MQ=255)
                                                                                               atttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctact                                             >  1:44522/1‑141 (MQ=255)
                                                                                                        ttATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGat                                    >  1:69941/1‑141 (MQ=255)
                                                                                                             cataatatgcgattCTGTTGAACCGATCAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCa                               <  2:498824/129‑1 (MQ=1)
                                                                                                              cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                              <  2:524564/133‑1 (MQ=255)
                                                                                                              cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                              >  1:28420/9‑141 (MQ=1)
                                                                                                              cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                              >  1:541637/9‑141 (MQ=11)
                                                                                                              cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                              >  2:40398/9‑141 (MQ=11)
                                                                                                              agtaaatgaGGTTCTGTTGCCAAGTTTAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAt                              <  1:314489/133‑1 (MQ=11)
                                                                                                              aaaaaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAACGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                              <  2:39227/140‑1 (MQ=255)
                                                                                                                gttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                            <  1:369284/135‑1 (MQ=9)
                                                                                                                 ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                           <  1:524731/136‑1 (MQ=2)
                                                                                                                 aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGa                           <  1:563822/136‑1 (MQ=2)
                                                                                                                 aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTAGCTAAGATATGCATTGa                           >  1:120563/6‑141 (MQ=2)
                                                                                                                  taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                          <  1:233363/137‑1 (MQ=9)
                                                                                                                  taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                          <  2:207550/137‑1 (MQ=9)
                                                                                                                  taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                          <  2:549279/137‑1 (MQ=9)
                                                                                                                  aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                          <  1:337501/137‑1 (MQ=2)
                                                                                                                  aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                          >  2:553635/5‑141 (MQ=2)
                                                                                                                  aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGCCTACTATCTAAGATATGCATTGAg                          >  2:555053/5‑141 (MQ=2)
                                                                                                                  aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTAAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAGGATATGCATTGAg                          >  2:367056/5‑141 (MQ=11)
                                                                                                                   aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                         <  1:569225/138‑2 (MQ=9)
                                                                                                                   aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                         <  1:310155/138‑2 (MQ=9)
                                                                                                                    ggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                        <  1:79436/139‑3 (MQ=9)
                                                                                                                    ataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGtt                        >  2:267952/1‑141 (MQ=38)
                                                                                                                     taGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTa                       >  1:608031/1‑141 (MQ=38)
                                                                                                                     gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGTTGTTGGCTACTATCTAAGATATGCATTGAGTTa                       >  1:182319/2‑141 (MQ=2)
                                                                                                                     gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATATAAGAGATGCATTGAGTTa                       >  2:432402/2‑141 (MQ=0)
                                                                                                                     gaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgcta                       >  1:201234/2‑138 (MQ=2)
                                                                                                                      aGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGTAATATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTGTCTAAGATATGCATTGAGGTAt                      >  1:118596/1‑141 (MQ=1)
                                                                                                                      aGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTAt                      >  2:35738/1‑141 (MQ=0)
                                                                                                                      aGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCCCTGTAGCCGTTCGATACTATCTACGATCTGCCTTGAGTGAt                      >  2:514170/1‑141 (MQ=11)
                                                                                                                       ggTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATc                     <  1:92884/141‑1 (MQ=35)
                                                                                                                       agTTCGGTGGCAAAGTTGAATCGGGAGTAGAATTGTAACAAAAAGGAGTCTTCTGTTTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATc                     <  1:284666/140‑1 (MQ=11)
                                                                                                                            tGTTGCAAAGTTGAATGTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGGTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGat                >  1:398555/1‑141 (MQ=17)
                                                                                                                            tGTTACAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGat                <  2:81576/141‑1 (MQ=25)
                                                                                                                              tttcAAAGTTTAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTATATGAACTATTTCAGATATAAACAATTTAACAAAGGTTTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGatat              <  2:605721/138‑1 (MQ=1)
                                                                                                                              ttGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatat              >  1:281001/1‑141 (MQ=35)
                                                                                                                                gCAAAGTTGAATCTATAGGATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatatat            >  2:61211/1‑141 (MQ=21)
                                                                                                                                   aaGTTGAATCTATAGCATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTg         >  2:574360/1‑141 (MQ=25)
                                                                                                                                    aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa        >  1:459923/1‑141 (MQ=35)
                                                                                                                                    aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTGCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa        <  1:254338/141‑1 (MQ=25)
                                                                                                                                    aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCAGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa        <  1:473210/141‑1 (MQ=25)
                                                                                                                                       tGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAat     <  2:254148/141‑1 (MQ=34)
                                                                                                                                          aTCTATAGTATAATTTTAACAGAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTCAGATATGCATTGAGTTATCGTGGTATATCTGAAGTAtt  >  1:346279/1‑141 (MQ=255)
                                                                                                                                          aTCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATAtt  >  1:326516/1‑141 (MQ=35)
                                                                                                                                            |                                                                                                                                          
TGAATTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATT  >  CP000730/76040‑76318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: