Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,185 G→A 17.3% intergenic (+243/‑891) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,1850GA17.3% 216.2 / 34.3 103intergenic (+243/‑891)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (49/36);  new base A (6/12);  total (55/48)
Fisher's exact test for biased strand distribution p-value = 7.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01

Only 100 of 114 total aligned reads displayed.

AATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGA  >  CP000730/76047‑76324
                                                                                                                                          |                                                                                                                                           
aaTATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGta                                                                                                                                           <  2:284609/141‑1 (MQ=255)
aaTATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGAGTTAGTAATCCAGGTACTACTAACACAAAGGCAATTAATTTAAAAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGta                                                                                                                                           <  2:194544/141‑1 (MQ=255)
   ataACTAATATTGCTGCACCTGTATCACTTCGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTACTAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGACTCTATAGTATaa                                                                                                                                        <  2:491584/141‑1 (MQ=255)
     aaCTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAtt                                                                                                                                      >  1:602122/1‑141 (MQ=255)
           tatTGCTGCACCTGTATCACTTAGAAAAGCTGCCCAAGGTGATAGAGAGCGAGGGAATACTGAGACGAAGGGGAGTGATGTAAGAATTAGAGCAACTGTTAGGGTCGGTTGCACAGGTGAATCTACAGGAAACttttgaaa                                                                                                                                >  2:70113/1‑137 (MQ=255)
             ttGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATGATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTaaaaaa                                                                                                                              >  2:254338/1‑141 (MQ=255)
                   cACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTACAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAg                                                                                                                        >  1:81576/1‑141 (MQ=255)
                    aCCTGTTTCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACGAACACATAGGCAATTAATTTAATAATTATAGCATATTATTGGTTTTGTTGCAAAGGTGAATCTATAGTATAATTTTAACAAAAAGGAGt                                                                                                                       <  2:441429/141‑1 (MQ=38)
                     ccTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtc                                                                                                                      >  2:92884/1‑141 (MQ=255)
                       tGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtctt                                                                                                                    <  1:504653/141‑1 (MQ=255)
                        gTATCACTTAGAACAGGTAACGAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAGTTTTAACAAAAAGAAGgcttc                                                                                                                   >  1:343857/1‑141 (MQ=38)
                             aCTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTAt                                                                                                              >  1:496913/1‑141 (MQ=255)
                             aCTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGCTTTCAGTAt                                                                                                              >  2:31412/1‑141 (MQ=255)
                                tAGGACATCTAACCAAAGTGTTAGTAGTCCAGGGAATACGAACACAAAGGCAATTAATTTAATAATTATAGCGAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCGTCTGTATGaa                                                                                                           <  2:313260/141‑1 (MQ=37)
                                 aGAACAGCTAACCAAAGTGTTACGAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAAc                                                                                                          <  1:87189/141‑1 (MQ=255)
                                  gAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACt                                                                                                         >  1:598675/1‑141 (MQ=255)
                                     cAGCTAACTAAAGTGTTAGTAATCCAGGGAATACTACCACAAAGCCAATTAATTTAATAATTATAGCAAAATATAGGTTGTGTTGCAAAGTTGAATCGATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAtt                                                                                                      <  2:57972/141‑1 (MQ=37)
                                     cAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAAGACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGGTGCAAAGTTGAATCTATAGAATAATTTTAACAAAACTGAGTCTTCTGAATGAACGAtt                                                                                                      >  2:498507/1‑141 (MQ=255)
                                     cAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAtt                                                                                                      >  1:559443/1‑141 (MQ=255)
                                       gCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTc                                                                                                    <  2:552835/141‑1 (MQ=255)
                                          aaCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGa                                                                                                 <  1:282016/141‑1 (MQ=255)
                                          aaCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGa                                                                                                 >  2:511566/1‑141 (MQ=255)
                                           aCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAACAAGGAGTCTTCTGTATGAACTATTTCAGat                                                                                                >  1:255164/1‑141 (MQ=255)
                                           aCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAgga                                                                                                >  1:210876/1‑139 (MQ=255)
                                           aCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGATCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAGAAAGGAGTCTTCTGTATGAACTATTTCAGat                                                                                                >  1:108027/1‑141 (MQ=255)
                                               aaGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATaa                                                                                            <  1:581292/141‑1 (MQ=255)
                                                aGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATaaa                                                                                           <  1:259586/141‑1 (MQ=255)
                                                     tAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAATAGTTGTCTGCTGTATGAACTATTTCAGATATAAACAAtt                                                                                      >  1:622334/1‑141 (MQ=255)
                                                     tAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGCGTCTTCCGTATGAACTATTTCAGATATAAACAAtt                                                                                      >  1:618928/1‑141 (MQ=255)
                                                      aGTAATCCAGGGAATACTAACACAAATGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAttt                                                                                     <  1:508452/141‑1 (MQ=255)
                                                      aGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCAGTTGCAAAGATGAATCTGTAGTATAATGTTAACAAAAAGGAGGCTTCTGTATGAACTATTTCAGATATAAACAAttt                                                                                     >  2:222742/1‑141 (MQ=25)
                                                          aTCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACa                                                                                 >  2:8503/1‑141 (MQ=255)
                                                            ccAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAg                                                                               >  1:542632/1‑141 (MQ=255)
                                                             cAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATg                                                                                                             >  1:632330/1‑110 (MQ=255)
                                                             cAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATg                                                                                                             <  2:632330/110‑1 (MQ=255)
                                                               gggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGAt                                                                            >  1:184015/1‑141 (MQ=255)
                                                                ggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATg                                                                           <  1:38296/141‑1 (MQ=255)
                                                                ggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATg                                                                           >  1:426699/1‑141 (MQ=255)
                                                                ggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATg                                                                           >  1:353690/1‑141 (MQ=255)
                                                                 gAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGt                                                                          <  1:614740/141‑1 (MQ=255)
                                                                  aaaaCTAACAAAAAGGGAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGtt                                                                         <  2:333569/138‑1 (MQ=255)
                                                                      cTAACACAAAGGCAATTAATTTAATAATTCTAGAAAAATATAGGACCTGATGCAAAGTTGGATCTATAGTATTAGTTTAATAATAAGGAGTCTTCGGTATGTACTATTTCAGATATAAAGAATTTAATAAGGATGTTATCa                                                                     >  2:131571/1‑141 (MQ=255)
                                                                        aaCACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACt                                                                   <  2:85143/141‑1 (MQ=255)
                                                                         acacAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTg                                                                  <  2:396282/141‑1 (MQ=255)
                                                                              aaGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                             <  2:553893/141‑1 (MQ=255)
                                                                                     ttaatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGct                                                      >  2:27230/1‑141 (MQ=255)
                                                                                        atttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctact                                                   >  1:44522/1‑141 (MQ=255)
                                                                                                 ttATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGat                                          >  1:69941/1‑141 (MQ=255)
                                                                                                      cataatatgcgattCTGTTGAACCGATCAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCa                                     <  2:498824/129‑1 (MQ=1)
                                                                                                       cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                    >  1:28420/9‑141 (MQ=1)
                                                                                                       cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                    <  2:524564/133‑1 (MQ=255)
                                                                                                       cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                    >  2:40398/9‑141 (MQ=11)
                                                                                                       agtaaatgaGGTTCTGTTGCCAAGTTTAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAt                                    <  1:314489/133‑1 (MQ=11)
                                                                                                       aaaaaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAACGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                    <  2:39227/140‑1 (MQ=255)
                                                                                                          ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                                 <  1:524731/136‑1 (MQ=2)
                                                                                                          aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGa                                 <  1:563822/136‑1 (MQ=2)
                                                                                                          aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTAGCTAAGATATGCATTGa                                 >  1:120563/6‑141 (MQ=2)
                                                                                                           taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                                <  1:233363/137‑1 (MQ=9)
                                                                                                           taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                                <  2:207550/137‑1 (MQ=9)
                                                                                                           taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                                <  2:549279/137‑1 (MQ=9)
                                                                                                           aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                                <  1:337501/137‑1 (MQ=2)
                                                                                                           aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                                >  2:553635/5‑141 (MQ=2)
                                                                                                           aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGCCTACTATCTAAGATATGCATTGAg                                >  2:555053/5‑141 (MQ=2)
                                                                                                           aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTAAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAGGATATGCATTGAg                                >  2:367056/5‑141 (MQ=11)
                                                                                                            aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                               <  1:310155/138‑2 (MQ=9)
                                                                                                            aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                               <  1:569225/138‑2 (MQ=9)
                                                                                                             ggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                              <  1:79436/139‑3 (MQ=9)
                                                                                                             ataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGtt                              >  2:267952/1‑141 (MQ=38)
                                                                                                              gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGTTGTTGGCTACTATCTAAGATATGCATTGAGTTa                             >  1:182319/2‑141 (MQ=2)
                                                                                                              gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATATAAGAGATGCATTGAGTTa                             >  2:432402/2‑141 (MQ=0)
                                                                                                              gaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgcta                             >  1:201234/2‑138 (MQ=2)
                                                                                                               aGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGTAATATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTGTCTAAGATATGCATTGAGGTAt                            >  1:118596/1‑141 (MQ=1)
                                                                                                               aGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTAt                            >  2:35738/1‑141 (MQ=0)
                                                                                                               aGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCCCTGTAGCCGTTCGATACTATCTACGATCTGCCTTGAGTGAt                            >  2:514170/1‑141 (MQ=11)
                                                                                                                ggTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATc                           <  1:92884/141‑1 (MQ=35)
                                                                                                                     tGTTGCAAAGTTGAATGTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGGTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGat                      >  1:398555/1‑141 (MQ=17)
                                                                                                                     tGTTACAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGat                      <  2:81576/141‑1 (MQ=25)
                                                                                                                       ttGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatat                    >  1:281001/1‑141 (MQ=35)
                                                                                                                         gCAAAGTTGAATCTATAGGATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatatat                  >  2:61211/1‑141 (MQ=21)
                                                                                                                            aaGTTGAATCTATAGCATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTg               >  2:574360/1‑141 (MQ=25)
                                                                                                                             aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa              >  1:459923/1‑141 (MQ=35)
                                                                                                                             aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTGCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa              <  1:254338/141‑1 (MQ=25)
                                                                                                                             aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCAGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa              <  1:473210/141‑1 (MQ=25)
                                                                                                                                tGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAat           <  2:254148/141‑1 (MQ=34)
                                                                                                                                   atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCCTTGAGCTATCGTGATATATCTGCAATAtt        >  1:62764/4‑141 (MQ=1)
                                                                                                                                   atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt        >  1:63015/4‑141 (MQ=2)
                                                                                                                                   atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt        >  2:316892/4‑141 (MQ=0)
                                                                                                                                   atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGAAATGCATTGAGCAATCGTGATATATATGGAATAtt        >  2:156656/4‑141 (MQ=1)
                                                                                                                                   aTCTATAGTATAATTTTAACAGAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTCAGATATGCATTGAGTTATCGTGGTATATCTGAAGTAtt        >  1:346279/1‑141 (MQ=255)
                                                                                                                                   aTCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATAtt        >  1:326516/1‑141 (MQ=35)
                                                                                                                                    tttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATGTCTGAAATATTa       <  1:472470/139‑1 (MQ=9)
                                                                                                                                     ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa      <  2:333149/140‑1 (MQ=12)
                                                                                                                                     ttataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTaa      <  1:659973/140‑1 (MQ=17)
                                                                                                                                      tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGACCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAg     <  1:527840/141‑1 (MQ=12)
                                                                                                                                      tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGCAATATTAAg     >  2:104434/1‑141 (MQ=12)
                                                                                                                                      tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAg     >  1:501789/1‑141 (MQ=12)
                                                                                                                                       ataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGTTATATCTGAAATATTAAgg    >  2:503125/1‑141 (MQ=17)
                                                                                                                                        taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAggg   >  1:256160/1‑141 (MQ=17)
                                                                                                                                         aGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGa  <  1:529675/141‑1 (MQ=32)
                                                                                                                                         aGTAAAATTTTAACAAAAAGGAGTCTAACATCTGACCTAAACCACAATTAAACGATATAAACAGGACGTTATCACTGTAGCTGTTGCCGACTATCCAAGTTAGGCATGGTGGTATCGTGATATATCTGAAATATTAAGGGa  <  2:321580/141‑1 (MQ=2)
                                                                                                                                          |                                                                                                                                           
AATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGA  >  CP000730/76047‑76324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: