Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,353 G→A 48.5% intergenic (+411/‑723) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,3530GA48.5% 70.2 / inf 340intergenic (+411/‑723)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (86/88);  new base A (83/81);  total (170/170)
Fisher's exact test for biased strand distribution p-value = 9.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 340 total aligned reads displayed.

ATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCAT  >  CP000730/76215‑76491
                                                                                                                                          |                                                                                                                                           
aTGAATTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTc                                                                                                                                           <  1:245558/141‑1 (MQ=17)
aTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTc                                                                                                                                           <  1:656592/141‑1 (MQ=17)
aTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTc                                                                                                                                           <  2:83380/141‑1 (MQ=17)
   aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAc                                                                                                                                        <  2:349045/141‑1 (MQ=17)
    aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAcc                                                                                                                                       <  1:402472/141‑1 (MQ=17)
    aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                                                                                                       <  1:629438/141‑1 (MQ=255)
    aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                                                                                                       >  2:516439/1‑141 (MQ=12)
      tATTTCATGTATAAACAATTTAACCAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                                                                                                                     <  2:571547/141‑1 (MQ=11)
      tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGt                                                                                                                                     <  2:377452/141‑1 (MQ=17)
      tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                                                                                                                     >  1:378813/1‑141 (MQ=12)
       aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGACCGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                                                                                    <  1:191533/141‑1 (MQ=255)
        tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTg                                                                                                                                   >  1:152817/1‑141 (MQ=12)
         ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTgg                                                                                                                                  <  1:145388/141‑1 (MQ=255)
         ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCGGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTgg                                                                                                                                  >  1:166754/1‑141 (MQ=12)
          tcggATACAAACAATTTAACAAGGATGTTATCACTCTATTTCTGCTCTACTAGCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTAATCATTCAACGATCTACCGTTggg                                                                                                                                 <  2:302909/138‑1 (MQ=11)
          tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCACCGGTCTACCGTTggg                                                                                                                                 >  2:461403/1‑141 (MQ=17)
           cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTGAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGACCGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGt                                                                                                                                >  2:578743/1‑141 (MQ=17)
           cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGt                                                                                                                                >  1:113826/1‑141 (MQ=12)
              atatAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCa                                                                                                                             <  2:43411/141‑1 (MQ=12)
                tatagatgtttacgaaGGTTGTTATCACTGTAGTTGTTGGCTACTATCTAATATATGGATTGGGTTATCGTGATATAGCTGAAATATTAAGGGAACGTTGTGTAAACGTTCATCATTCAACGGTTTACCGTTGGGTTCAAg                                                                                                                           <  2:546745/127‑1 (MQ=11)
                ataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAg                                                                                                                           <  1:438937/141‑1 (MQ=17)
                 taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                                                                                                          >  2:214800/1‑141 (MQ=17)
                 taAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                                                                                                          <  1:86599/141‑1 (MQ=255)
                   aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAGTAGTAAGGGAACGTGGTGTAAACGTTCATCATTCCACGGTCTACCGTTGGGCTCACGAat                                                                                                                        >  1:78900/1‑141 (MQ=11)
                    aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGCATTATTAAGAGAACGTGGTGTAAACGTTCATCATTCTACGATCTACCGTTGGGTTCAAGAata                                                                                                                       >  1:608846/1‑141 (MQ=11)
                     cAATTTAACAAGGATGTTATCACTGTAGCCGTTGTCTACTATCTAAGATGTGCATTGAGCTATCGTGATATAGCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAatat                                                                                                                      >  2:176435/1‑141 (MQ=11)
                     cAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAatat                                                                                                                      >  2:40651/1‑141 (MQ=12)
                     aaaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAGCGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAatat                                                                                                                      <  2:314782/140‑1 (MQ=255)
                      aaTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATg                                                                                                                     >  1:268400/1‑141 (MQ=17)
                      aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATg                                                                                                                     <  2:653543/141‑1 (MQ=12)
                       aTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGc                                                                                                                    <  1:27230/141‑1 (MQ=32)
                       aTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCCTCATTCACCGATCTACCGCTGGGTTCAAGAATAAGc                                                                                                                    >  2:189269/1‑141 (MQ=11)
                        tttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcc                                                                                                                   <  1:504643/141‑1 (MQ=32)
                        tttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcc                                                                                                                   <  2:525867/141‑1 (MQ=32)
                         ttAACGAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGAGATGCATTGAGCTATCGTGATATATCTGAAATAGTAAGAGAACGTGGTGTAAACGTTCATCATTCAGCGAGCTAGCGGTGGGTTCAAGAAGATGccc                                                                                                                  <  2:389791/141‑1 (MQ=11)
                          tAACACGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcccc                                                                                                                 <  2:359563/141‑1 (MQ=255)
                          tAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGACATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGGGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGCTCAAGAATATGcccc                                                                                                                 >  1:648109/1‑141 (MQ=255)
                          tAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcccc                                                                                                                 >  1:601193/1‑141 (MQ=255)
                            aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                               >  1:627575/1‑141 (MQ=255)
                            aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                               >  2:399203/1‑141 (MQ=255)
                            aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                               >  2:68161/1‑141 (MQ=12)
                            aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCACTCAACGAGCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                               >  1:122512/1‑141 (MQ=11)
                            aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAATGAACTAACGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                               >  1:555416/1‑141 (MQ=11)
                               aGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAttt                                                                                                            >  2:281332/1‑141 (MQ=255)
                                 gATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTta                                                                                                          >  1:415148/1‑141 (MQ=18)
                                 gATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTta                                                                                                          >  2:591625/1‑141 (MQ=255)
                                  aTGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGGTCAAGAATATGCCCCAATTTtat                                                                                                         >  2:667994/1‑141 (MQ=21)
                                  aTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                                                                                                         <  2:608930/141‑1 (MQ=255)
                                    gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                                                                                       >  1:334475/1‑141 (MQ=255)
                                     ttATCACTGTAGCTGTTGGCTATTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                                                                                      <  2:256910/141‑1 (MQ=11)
                                       aTCACTGTAGCCGTTGGCTACTATATAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa                                                                                                    >  1:488001/1‑141 (MQ=255)
                                        tCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa                                                                                                   >  2:101316/1‑141 (MQ=25)
                                        tCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTAACGGGATATATGTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa                                                                                                   >  1:294061/1‑141 (MQ=11)
                                          aCTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAGTTTAATATCAAAtt                                                                                                 >  2:229346/1‑141 (MQ=11)
                                             ttAGCTGTTGGGTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                                                                              <  1:634023/140‑1 (MQ=25)
                                             gTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                                                                              >  2:538688/1‑141 (MQ=21)
                                              tAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                                                                             >  1:566536/1‑141 (MQ=35)
                                                gctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                                                                              <  1:450452/135‑1 (MQ=37)
                                                gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                                                                           <  2:479759/141‑1 (MQ=255)
                                                 gtgtcggCTACTATCTAAGATATGCATTGAGTTATCGTGATATATATGAAAAATAAAGGGAACGTGCTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaaaaaaaaaaa                                                                                          <  2:496913/136‑10 (MQ=11)
                                                 ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAGTATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAAGATGCCCCAATTTTATATCAAATTTGgaaga                                                                                          >  2:491595/1‑141 (MQ=11)
                                                  tgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaagaa                                                                                         <  1:135895/140‑1 (MQ=25)
                                                  cGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAAAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatatacaagtcggaaaaa                                                                                         >  2:621674/1‑127 (MQ=11)
                                                       gCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGc                                                                                    <  1:281332/141‑1 (MQ=255)
                                                        ttcctctccaatgtatGCATTGCGCTTTGGGGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGGTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                                                   <  2:166838/128‑1 (MQ=11)
                                                        ctactaTCTAAGATATGCATTTAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                                                   <  1:93692/141‑1 (MQ=25)
                                                        ctactaCTTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                                                   <  2:642830/141‑1 (MQ=11)
                                                        ctacgaTCTAAGATATGCATTCAGCTATCGGGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                                                   <  2:156166/141‑1 (MQ=11)
                                                        caactaTATAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGACAACGGGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCCCATTTGGAAGAAAAAGCa                                                                                   <  2:278703/139‑1 (MQ=11)
                                                         tactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                                 <  1:216659/141‑1 (MQ=34)
                                                         tactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATAAAATTTGGAAGAAAAAGCaa                                                                                  >  2:128795/1‑140 (MQ=25)
                                                         tactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                                 <  2:249535/141‑1 (MQ=255)
                                                         tactaTCTAAGATACGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                                 <  1:356569/141‑1 (MQ=255)
                                                         gactaTCTAAGATAGGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                                 <  2:298592/140‑1 (MQ=255)
                                                          actaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                                                                >  1:178821/1‑141 (MQ=35)
                                                          actaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                                                                <  1:262072/141‑1 (MQ=255)
                                                            taTCTAAGATATGGAGTGAGTTCTTGTGATATATCTGAAATATTAAGAGATCGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa                                                                              <  2:559762/141‑1 (MQ=11)
                                                             aacTAAGATATGACTTGAGCTATCGTGATATATATAAAATAATAAGAGAACATGGTGTAAACGTTCATCATTCAACTATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTTGAAGAAAAAGCATaaaa                                                                             <  2:600177/139‑1 (MQ=11)
                                                               ttAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGTAAATGCATaaaaaa                                                                           <  2:452461/140‑2 (MQ=11)
                                                               tgaaGGTATGTATTGAGGTATCGTGATAGATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaa                                                                           <  2:444428/139‑2 (MQ=11)
                                                                  aGATGTGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGACCCAAATTTATATCAAATTTGGAAGAAGAAGCATaaaaaagct                                                                        >  1:470441/1‑137 (MQ=11)
                                                                  aGATATGCATTGAGCTATCGTGCCTCTGCGTAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct                                                                        >  1:661998/1‑137 (MQ=11)
                                                                    atatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagctta                                                                      <  2:139152/141‑7 (MQ=11)
                                                                     tatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat                                                                     <  1:529147/141‑8 (MQ=1)
                                                                     tatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat                                                                     >  2:22287/1‑134 (MQ=11)
                                                                      atGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTa                                                                    >  2:270975/1‑141 (MQ=40)
                                                                                     gTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATtga                                                     <  1:222742/141‑1 (MQ=39)
                                                                                       gATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGTATATGCCCCAATCTTATATCAAATTTGGAAGAACAAGCATTAAAAAGCTGGTTACAAATTGCGTATggat                                                    >  1:33877/1‑141 (MQ=1)
                                                                                            ttCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACg                                              <  1:574360/140‑1 (MQ=39)
                                                                                            atCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACg                                              >  1:28660/1‑141 (MQ=39)
                                                                                                  aaTATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGACTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACacc                                        >  2:12363/1‑139 (MQ=255)
                                                                                                       tAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCCAATTTGGGAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACATACACaaaagt                                  >  2:513467/1‑139 (MQ=14)
                                                                                                        aaGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATa                                  >  2:606795/1‑141 (MQ=40)
                                                                                                        aaGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATa                                  >  2:340738/1‑141 (MQ=38)
                                                                                                             agcGTGGTGTAAACGTGCATCATAGAACGGAATACTGGTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGATGTACATCAAAATAAAAgg                             <  1:514170/139‑1 (MQ=14)
                                                                                                                             tcatcaTTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTCGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTta             >  2:74668/1‑141 (MQ=255)
                                                                                                                               atcatTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTtata           >  1:589112/1‑141 (MQ=38)
                                                                                                                                   ttCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGt       <  1:545556/141‑1 (MQ=39)
                                                                                                                                     aaaCGGTCCACCGTTGGGTTCAAGAACATGCCCCAATTTTATATCAAATTGGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGc     <  2:353690/140‑1 (MQ=255)
                                                                                                                                        cGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCAt  <  1:235743/141‑1 (MQ=39)
                                                                                                                                          |                                                                                                                                           
ATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCAT  >  CP000730/76215‑76491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: