Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 707,879 A→T 100% L11I (TTA→ATA)  USA300HOU_0653 ← ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730707,8790AT100.0% 151.9 / NA 54L11I (TTA→ATA) USA300HOU_0653ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (26/28);  total (26/28)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TACCGGTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATA  >  CP000730/707741‑708007
                                                                                                                                          |                                                                                                                                
tACCGGTAGCATTAAATTCACCAATTAAAGACTTGATAAGTGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTataa                                                                                                                                >  1:476236/1‑141 (MQ=255)
tACCGGAAGCGTAAAATACACCAATGACAAAGTGGGTAAGGGAAGATTTACCAGGCCCATTCGGGCCCATGATACCAATGATTTCGCCGCGTACTGGTATCGAAAAGGAAATGATTTTACATACATGCTTATTACCTataa                                                                                                                                >  2:323475/1‑141 (MQ=255)
      tAGCATTATATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTGCTGGTATCGATAAGGAAATGTTTCTAAGTACATGCTTATTACCTATAAACAGAt                                                                                                                          >  2:491668/1‑141 (MQ=255)
        gCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGAttt                                                                                                                        >  1:627494/1‑141 (MQ=255)
              aaTTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCGTATTACCTGTAAACAGATTTAAATCt                                                                                                                  >  2:521299/1‑141 (MQ=255)
                ttCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAATAGATTTAAATCttt                                                                                                                <  2:453732/141‑1 (MQ=255)
                      aaTTAAAGACTTGATAAGGGAAGATTCACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTc                                                                                                          <  1:279831/141‑1 (MQ=255)
                           aagccctgatgaGGGAAGATCTACCAGCACTATTCGGGCCCATGCTTCCAATTACTTCGCCGCGTACTGGTATCGCTAAGGAAATGTTTTTAAGTACATGCTGATTACCTATAGACAGATTTAAATCTTTTGTTTCTAACa                                                                                                     <  2:655333/135‑1 (MQ=255)
                              aCTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAAc                                                                                                  <  1:402229/141‑1 (MQ=255)
                                 tGATATGGGAAGACTTACCAGCCCCATGCGGGCCCATGATGCCAATTATTTCGCCGCGTACTGGTATTGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGtt                                                                                               <  2:579929/141‑1 (MQ=255)
                                 tGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGtt                                                                                               >  1:648762/1‑141 (MQ=255)
                                  gATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGttt                                                                                              <  2:536928/141‑1 (MQ=255)
                                  gATAAGGGAAGATGTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTTGGTTGGTGTTTTCTAACAAACGttt                                                                                              <  2:644550/141‑1 (MQ=255)
                                       ggggataTTCACCAGCCCCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATAcc                                                                                         <  2:49808/135‑1 (MQ=255)
                                       gggAAGAGTTACCAGCACCATTCGAGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAACATTTTGTTTCTAACAAACGTTTATAcc                                                                                         >  1:424507/1‑141 (MQ=255)
                                                 aCCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTaa                                                                               >  1:243492/1‑141 (MQ=255)
                                                 aCCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTaa                                                                               >  1:200849/1‑141 (MQ=255)
                                                  ccAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTCTTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTaaa                                                                              <  2:607845/141‑1 (MQ=255)
                                                  ccAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTAATGGTAGCGATAAGGAAATGCTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTaaa                                                                              >  1:129141/1‑141 (MQ=255)
                                                      cACCATGCGGGCCCATGATACCAATTATTTCGCCGCGCACTGGTATCGATAAGGTCATGTTGTTAAGTACATGCTTATTACCTTTAAACAGATTTAAATCTTTTGTTTCTGACAAACGTTTATCCGTGCTAGTTGAAAAtt                                                                          >  1:238481/1‑141 (MQ=255)
                                                              gggCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCTTAATTAAAAGtatattctga                                                                  >  2:635403/1‑135 (MQ=255)
                                                               gccccATGGTAATAATGATGTCGCCGCGTACTGGTATCGGGTAGGAAATAGTTTTACGGACATGCTTATTTCCTATACACAGATTTAAATGTAGTGTTTCTAACAAACGTTTATACCGCCTAATTAAGAGATTAGGCTAAc                                                                 <  2:109562/139‑1 (MQ=255)
                                                                gCCCATGATGCCAATTATTTCGCCGCGTCCAGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGACTAAcc                                                                >  1:490471/1‑141 (MQ=255)
                                                                 cctaTGCTAGCAAATATTTTTCCGCGTACTGGTATTGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCt                                                               <  2:519840/138‑1 (MQ=255)
                                                                 cccATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCt                                                               <  1:610593/141‑1 (MQ=255)
                                                                    aTGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaat                                                            >  2:222791/1‑141 (MQ=255)
                                                                          ccAATTATTTCCACGCGTACTGGTATCGATAAGGAAATGTTTCTAAGTACATGCTTCATACCTATAAACAGATTTAAATCTTTTGTTTCTACCACACGTTTCTACCTCCTAATTAAACTTTTAGGCTAACCtaatgatttt                                                      >  2:53924/1‑135 (MQ=255)
                                                                           ctattattTACCCGTGTACTGATATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGGTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGt                                                     <  2:151485/139‑1 (MQ=255)
                                                                              ttattTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGtata                                                  <  1:222791/141‑1 (MQ=255)
                                                                                    cgccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTAACTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGACTAACCTAATTAATTGTATAATAAAc                                            >  2:492837/1‑141 (MQ=255)
                                                                                     gccgcGTTCTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACt                                           <  2:384137/141‑1 (MQ=255)
                                                                                     gccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACt                                           >  1:617911/1‑141 (MQ=255)
                                                                                       cgcgTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTga                                         >  1:621026/1‑141 (MQ=255)
                                                                                         cgtatgggTAACGAAAAGTAAATGTTTTTAAGGACGAGCTGTTTACATATAAACAGAATTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGACTAACCTAATTAGTAGTATAATTAAcaaaaa                                       >  2:220926/7‑136 (MQ=255)
                                                                                              tGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATAtt                                  <  1:262/141‑1 (MQ=255)
                                                                                               ggTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATAttt                                 >  1:301600/1‑141 (MQ=255)
                                                                                                gTATCGATAAGGAAATTTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTa                                <  1:16375/141‑1 (MQ=255)
                                                                                                     gATAAAGAAATGTTTTTAAGTTCATGGTTATTGCCTATAGACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCCTATTAAAAGTTTAGGCTAACCCACTTAATTGTTTAATAAACTGAGAATATTCATCATg                           <  2:214033/141‑1 (MQ=255)
                                                                                                        aaGGAAATGTTTTTAAGTACACGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCa                        <  1:631454/141‑1 (MQ=255)
                                                                                                           gAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGt                     <  1:667070/141‑1 (MQ=255)
                                                                                                           gAAAGGTTTTTAAGTACATGTTTATTACCTATAAACAGATTTAAATCTCTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTTAGAATATTTATCATGTCAAGt                     <  2:200849/141‑1 (MQ=255)
                                                                                                           aaaaTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTTACGCATTGGTTTTTAACTAACGTTTATACCTCCTAATTACATGTTTAAGCTAACCTAATTAATTGTATAATAAACTAACAATATTTATCATGTCAAGt                     <  2:263464/140‑1 (MQ=255)
                                                                                                            aaaTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                    >  1:565572/1‑141 (MQ=255)
                                                                                                            aaaTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                    <  1:494485/141‑1 (MQ=255)
                                                                                                             aaTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaa                   <  2:627494/141‑1 (MQ=255)
                                                                                                               tGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAt                 >  2:460347/1‑141 (MQ=255)
                                                                                                                 tttttAAGTACATGCTTCTTCACTATTAACATATTTCTCTCATTGGCTTCCTGAAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTc               <  1:652143/141‑1 (MQ=255)
                                                                                                                  ttttAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCg              >  2:74280/1‑141 (MQ=255)
                                                                                                                  ttttAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTACGCTAACCTAATTAATTGTATAATAAACTGACAATATTTATCATGTCAAGTAAAttag              >  2:122843/1‑139 (MQ=255)
                                                                                                                  ttttAAGTACATGCTTATTACCTATAAACAGATGTAAATCTTTTATTTCTAACAACCGTTTAAGCCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCCTGTCAAGTAAATTCg              >  2:471692/1‑141 (MQ=255)
                                                                                                                   tttAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGt             >  2:608203/1‑141 (MQ=255)
                                                                                                                   tttAAGTACACGCTTATTACCTATAACCAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCGAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGt             <  1:42612/141‑1 (MQ=255)
                                                                                                                       aGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGata         <  2:332776/141‑1 (MQ=255)
                                                                                                                         tACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatata       >  2:551829/1‑141 (MQ=255)
                                                                                                                         tACATGCCTACCACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatata       >  2:646615/1‑141 (MQ=255)
                                                                                                                              gCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTATGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataatata  <  2:313969/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                
TACCGGTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATA  >  CP000730/707741‑708007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑