Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 864,289 A→G 7.9% intergenic (+20/‑47) USA300HOU_0807 → / → secG hypothetical membrane protein/possible Sec family Type I general secretory protein SecG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730864,2890AG 7.9% 128.8 / 3.7 51intergenic (+20/‑47)USA300HOU_0807/secGhypothetical membrane protein/possible Sec family Type I general secretory protein SecG
Reads supporting (aligned to +/- strand):  ref base A (23/24);  new base G (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.57e-01

AAAGCTGCACACGGTGCGTACTTAACATTGCCAATTTTAATTGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTAATCATTGATTGTATTGCATTAATAACTGTTGTACTACTCCAAGAAGGTAAAAGCAGTGGACTTTCAG  >  CP000730/864150‑864429
                                                                                                                                           |                                                                                                                                            
aaaGCTGCACACGGTGCGTACTTAGCATTGCCAATTTTAATTGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAAGCGCTAGAAAATTGATTTTAACAATaaa                                                                                                                                             >  1:425910/1‑141 (MQ=255)
aaaGCTGCACACGGTGCGTACTTAACATTGCCAATTTTAATTGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATaaa                                                                                                                                             <  1:267566/141‑1 (MQ=255)
   gCTGCACACGGTGCGTACTTAACATTGCCAATTTTAATTGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAat                                                                                                                                          <  2:586481/141‑1 (MQ=255)
      gCACACGGTGCGTACTTAACATTGCCAATTTTAATTGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATg                                                                                                                                       >  1:145571/1‑141 (MQ=255)
            ggTGCGTACTTAACATTGCCAATTTTAATTGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATAtac                                                                                                                                 <  1:624724/141‑1 (MQ=255)
            ggTGCGTACTTAACATTGCCAATTTTAATTGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATCATAtac                                                                                                                                 >  1:12541/1‑141 (MQ=255)
             gTGCGTACTTAACATTGCCAATTTTAATTGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATAtact                                                                                                                                <  1:48780/141‑1 (MQ=255)
             gTGCGTACTTAACATTGCCAATTTTAATTGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATAtact                                                                                                                                <  1:541998/141‑1 (MQ=255)
             gTGCGTACTTAACATTGCCAATTTTAATTGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATAtact                                                                                                                                <  2:585947/141‑1 (MQ=255)
              tGCGTACTTAACATTGCCAATTTTAATTGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATAtacta                                                                                                                               <  1:226162/141‑1 (MQ=255)
                             gCCAATTTTAATTGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAGCAATAAAAATATGATATACTACTTGTCGTATATAAg                                                                                                                >  1:388846/1‑141 (MQ=255)
                              ccAATTTTAATTGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGCTTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAgg                                                                                                               >  2:348751/1‑141 (MQ=255)
                              ccAATTTTAATTGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAgg                                                                                                               >  1:171774/1‑141 (MQ=255)
                                 aTTTTAATTGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTTTCGTATATAAGGacc                                                                                                            >  1:280695/1‑139 (MQ=255)
                                   tttAATTGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATGTAAGGGACgg                                                                                                          >  2:361866/1‑141 (MQ=255)
                                      aaTTGTTATTATAGGGTCGGTCTCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACggagg                                                                                                       <  2:153041/141‑1 (MQ=255)
                                       aTTGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACggagga                                                                                                      >  1:228541/1‑141 (MQ=255)
                                         tGTTATTATGTGTTGGTGTGGATTAGCGATTTATATGCTTGTTGTTTCTATCAATAGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAAGATGATATACTACTTGTCGTATATAAGGAACGGTGGACa                                                                                                    <  2:83723/141‑1 (MQ=255)
                                            tattatAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAAtt                                                                                                 <  1:311518/141‑1 (MQ=255)
                                              ttatAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTa                                                                                               >  1:218070/1‑141 (MQ=255)
                                                    ggtCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATa                                                                                         <  1:245048/139‑1 (MQ=255)
                                                       ctgTTGTATTAGCGATTTGTATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGACGACAATTTATGCATACAt                                                                                      <  2:324478/139‑1 (MQ=255)
                                                         gTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACAttt                                                                                    <  1:257822/141‑1 (MQ=255)
                                                          ttGTATTAGAGATTTATATGCTTGTTGGTGCTATCAAACGTAAAAGTACATTTAATCTCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACAtttt                                                                                   <  2:106621/141‑1 (MQ=255)
                                                          ttGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAAAACGATATACTACTTGTCGTATCTAAGGAACGGAGGACAATTTATGCATACAtttt                                                                                   >  2:366991/1‑141 (MQ=255)
                                                                  gCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGta                                                                           >  1:232632/1‑141 (MQ=255)
                                                                    gATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAGAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGtatt                                                                         >  2:471445/1‑141 (MQ=255)
                                                                    gATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAGAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGtatt                                                                         >  2:404767/1‑141 (MQ=255)
                                                                       ttATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTattatt                                                                      <  2:504677/141‑1 (MQ=255)
                                                                          tatGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTGAt                                                                   >  1:533295/1‑141 (MQ=255)
                                                                          tatGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTAAt                                                                   <  1:105818/141‑1 (MQ=255)
                                                                                 gttgttTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTAATCattgat                                                            <  1:487086/141‑1 (MQ=255)
                                                                                 gttgttTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTAATCattgat                                                            <  2:284571/141‑1 (MQ=255)
                                                                                   tgttGCTATCATACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTAATCattgattg                                                          <  2:232329/141‑1 (MQ=255)
                                                                                     tttCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACCTTTTTATTCGTATTATTAATCATTGATTGTa                                                        >  2:294452/1‑141 (MQ=255)
                                                                                      ttCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTAATCATTGATTGTAt                                                       >  2:279882/1‑141 (MQ=255)
                                                                                       tCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCTTATTTTTAATCATTGATTGTAtt                                                      >  1:481561/1‑141 (MQ=255)
                                                                                        cTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATGTGATATACTACTTGTCGGAGATAAGGAACGGAAGACAATTTATGAATACATTTTTAATCGTATTATTAATAATTGATGGTAttt                                                     >  2:1701/1‑140 (MQ=255)
                                                                                         tATCAAATGTAAAAGTACATTTAATCTTCAGAAAATTGATTTTAACAATAAAAAAATGATATACTACTTGTTTTATATAAGGAACGGAGGAAAATTTATGCATACATTTTTAATCGTATTATTAATCATTGATTGTATTGc                                                    <  2:500365/141‑1 (MQ=255)
                                                                                          aTCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTAATCATTGATTGTATTGCa                                                   >  1:560333/1‑141 (MQ=255)
                                                                                           tCAAACGTAAAAGTATATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTAATCATTGATTGTATTGCAt                                                  <  2:77243/141‑1 (MQ=255)
                                                                                            cAAACGTAAAAGTACGTTTAATCGCTCGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATTCATACATTTTTAATCGTATTATTCATCATTGATTGTATTGCAtt                                                 <  1:406293/141‑1 (MQ=255)
                                                                                               aCGTAAAAGTAAATCTAATCGTCAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAAGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTAATCATTGATTGTATTGCATtaat                                              <  2:388846/141‑1 (MQ=255)
                                                                                                cGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTAATCATTGATTGTATTGCATtaata                                             <  2:652962/141‑1 (MQ=255)
                                                                                                   aaaaGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTAATCATTGATTGTATTGCATTAATAACt                                          >  1:274947/1‑141 (MQ=255)
                                                                                                      aGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTAATCATTGATTGTATTGCATTAATAACtgtt                                       >  1:536831/1‑141 (MQ=255)
                                                                                                          aaTTTAATCGCTAGAAAATTGACTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGATCGGAGGACAATTTATGTATACATTTTTAATCGTATTATTAATCATTGATTGTATTGCATTAATAACTGTTGtac                                   <  2:154898/140‑1 (MQ=255)
                                                                                                                       gAAAATTGATTTTAACAATAGAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTAATCATTGATTGTATTGCATTAATAACTGTTGTACTACTCCAATAAgg                      <  1:471445/141‑1 (MQ=255)
                                                                                                                       gAAAATTGATTTTAACAATAGAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTAATCATTGATTGTATTGCATTAATAACTGTTGTACTACTCCAAGAAgg                      <  1:404767/141‑1 (MQ=255)
                                                                                                                          aaTTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTAATCATTGATTGTATTGCATTAATAACTGTTGTAATACTCCAAGGAGGTaa                   >  1:335229/1‑141 (MQ=255)
                                                                                                                          aaTTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAAGACACTTTATGCATACATTTTTAATCGTATTATTAATCATTGATTGTATTGCATTTATAACTTTTGTAGTATTCCAAGAAGGTaa                   >  2:189943/1‑141 (MQ=255)
                                                                                                                                ttttAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTAATCATTGATTGTATTGCATTAATAACTGTTGTACTACTCCAAGAAGGTAAAAGCAg             >  2:88012/1‑141 (MQ=255)
                                                                                                                                 tttAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTAATCATTGATTGTATTGCATTAATAACTGTTGTACTACTCCAAGAAGGTAAAAGCAGt            >  1:494210/1‑141 (MQ=255)
                                                                                                                                          aaaaaTATGATATACTACTTGCCGTCTATAAGGAACGGAGGACAATTTATGCATACATTGTTAATCGTATTATTAATCATAGCTTGTATTGCATTAATAACTGTTGTGCTACTCCAAGAAGGTAACATCGATGGACtttac   >  2:372239/1‑139 (MQ=255)
                                                                                                                                           aaaaTATGATATACTACTTGTCGTATATAAGGAACGGAGCACACAATTTTTTTACATTTTTAATCGTATTATTAATCATTGATTGTATTGCATTAATAACTGTTGTACTACTCCAAGAAGGTAAAAGCAGTGGACTTTCAg  >  1:628300/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
AAAGCTGCACACGGTGCGTACTTAACATTGCCAATTTTAATTGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTTGTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAGAAAATTGATTTTAACAATAAAAATATGATATACTACTTGTCGTATATAAGGAACGGAGGACAATTTATGCATACATTTTTAATCGTATTATTAATCATTGATTGTATTGCATTAATAACTGTTGTACTACTCCAAGAAGGTAAAAGCAGTGGACTTTCAG  >  CP000730/864150‑864429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: