Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,416,4580AT100.0% 112.2 / NA 37I216N (ATT→AAT) USA300HOU_1314oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (20/17);  total (20/17)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTC  >  CP000730/1416329‑1416594
                                                                                                                                 |                                                                                                                                        
cAATCCTAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACaa                                                                                                                               <  2:115837/141‑1 (MQ=255)
           aTCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATAc                                                                                                                    <  2:541817/141‑1 (MQ=255)
              ccTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATg                                                                                                                 <  1:517917/141‑1 (MQ=255)
                     aaTTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACATCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAg                                                                                                          >  2:237403/1‑141 (MQ=255)
                        tgtgTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATGTTTCAATACATGCCGTCAGTTa                                                                                                       >  1:214674/1‑141 (MQ=255)
                             tCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAACATTTCAATACATGCCTTCAGTTAtgtgt                                                                                                  >  2:148387/1‑141 (MQ=255)
                                   tttAATGAGGCGGTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGataat                                                                                            <  2:202678/141‑1 (MQ=255)
                                       aTTAGACGTTCAGTATACTGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATaataaat                                                                                        <  2:36915/141‑1 (MQ=255)
                                          aGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATCTCAATACATGACTTAAGTTATGTGTGATAATAaataat                                                                                     >  1:434546/1‑141 (MQ=255)
                                           gACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTTTAATAATAaataatt                                                                                    >  2:199547/1‑140 (MQ=255)
                                            aCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGt                                                                                   <  2:662951/141‑1 (MQ=255)
                                            aCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGt                                                                                   >  1:233012/1‑141 (MQ=255)
                                            aCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTAATGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGt                                                                                   >  2:437345/1‑141 (MQ=255)
                                            aCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATCACCCCGTTCAATGCTTTTACCTTGTTTTAAAACATAATTGTAGTGACAATATTTCGATACATGACTTAAGTTATGTGTGATAATAAATAATGt                                                                                   >  2:435705/1‑141 (MQ=255)
                                                  aGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAcc                                                                             <  1:354750/141‑1 (MQ=255)
                                                            tGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAAt                                                                   <  2:105469/141‑1 (MQ=255)
                                                               aaaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACa                                                                <  2:628756/141‑1 (MQ=255)
                                                                aaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAGTTGTACTGACAATATTTGAATACATGAATTAAGTTATGTGGGATAATAAATAATGTTTGAACATGTTCTAATaaaa                                                               >  2:403445/1‑141 (MQ=255)
                                                                 aTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGAAAATATTTCAATACATGACTTAAGTTATGTGTGTTATTAAATATTGTTTTACCTTGTTataatattat                                                              >  1:7754/1‑137 (MQ=255)
                                                                 aTGCTCATACTTGAAATGATTAATATTAATTCGTTCAATGATTTGAACTTCTTTTAAAACATAATTGTCCTGCCAATATTTCAATACATGACTTAAGTTACGTGTGATAACAAAGAATGTTTGACCATGTTCTCATACAat                                                              >  2:425518/1‑141 (MQ=255)
                                                                     tCATTCTTTAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGc                                                          <  2:65068/141‑1 (MQ=255)
                                                                              aaaTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATaaa                                                 >  2:39098/1‑141 (MQ=255)
                                                                               aaTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAAt                                                >  1:168475/1‑141 (MQ=255)
                                                                                    ttAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATc                                           >  1:235480/1‑141 (MQ=255)
                                                                                    ttAATATTACCTCGGTCAGTGATTTGACCTTCTGTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATc                                           <  1:208221/141‑1 (MQ=255)
                                                                                      aaTATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATCCATGACTTAAGTTATGTGTGCTAATAAATAATGTTTAACCATGTTCTAATACAATATACTGTAATAAATCCTTccc                                         >  2:513583/1‑139 (MQ=255)
                                                                                      aaTATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCAATAAc                                         >  2:657250/1‑141 (MQ=255)
                                                                                         atTGCCTCGTTCGATTGTGTGACCGGCGTTTAAAGCATGATTGTACTGACAATATTGCAAGACATGACTTAAGTGAGGTGTGATAATAAGTAATGGTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTg                                      <  2:634434/141‑1 (MQ=255)
                                                                                              cTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTAATGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTGGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTAc                                 <  1:57249/141‑1 (MQ=255)
                                                                                               tCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTAcc                                <  1:453609/141‑1 (MQ=255)
                                                                                                       tGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATCATAAATAATGTTTGACGATGTTCTAATACAATATGCTGTAATAAATCTATCACTTGATTAGCGGTCAAAg                        >  2:297808/1‑141 (MQ=255)
                                                                                                                 ttcttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAatga              >  1:585071/1‑141 (MQ=255)
                                                                                                                 ttcttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAatga              <  1:249831/141‑1 (MQ=255)
                                                                                                                  tcttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTCCCGTTCAAAGCATCCAatgat             >  2:262499/1‑141 (MQ=255)
                                                                                                                       tAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATAAGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAAc        >  1:624968/1‑141 (MQ=255)
                                                                                                                          aaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTgg     <  1:361670/141‑1 (MQ=255)
                                                                                                                             aTAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTc  <  1:214112/141‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                        
CAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTC  >  CP000730/1416329‑1416594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: