Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | CP000730 | 1,744,454 | C→T | 100% | G73D (GGC→GAC) | apt ← | adenine phosphoribosyltransferase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | CP000730 | 1,744,454 | 0 | C | T | 100.0% | 167.8 / ‑6.5 | 57 | G73D (GGC→GAC) | apt | adenine phosphoribosyltransferase |
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (27/29); minor base A (1/0); total (28/29) | |||||||||||
Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTA > CP000730/1744319‑1744588 | tcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATc < 1:354804/141‑1 (MQ=255) aTTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCAtt < 2:251205/141‑1 (MQ=255) aTTAACACACGGTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCAtt < 1:211076/141‑1 (MQ=255) acacacGTTGACCTGGTTTAATTGCCTCTTTGTGCGTTGTTAAAACATTTGTAGCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATGTCCCTTCTTTTCTAACAGGTGCAAAGACAATCCCCCTTgcac > 2:126446/1‑138 (MQ=255) acacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATaa > 2:311544/1‑141 (MQ=255) cacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAg > 1:181864/1‑141 (MQ=255) ccGTTGACCTGGTTTAATTGCATCTTCGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGc < 2:115413/140‑1 (MQ=255) cGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCt > 2:653478/1‑141 (MQ=255) gTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTa > 1:209922/1‑141 (MQ=255) gACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAg < 1:311544/141‑1 (MQ=255) aCCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCCAAGTCAATCCCCATTGAATAAGCTACAgg > 2:377413/1‑141 (MQ=255) tAATTGCATCTTTGTGCATTGTTTAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCaa < 1:302449/141‑1 (MQ=255) ttGCATCTTTGAGCATTGTTAAAGCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGa < 1:522480/141‑1 (MQ=255) tCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAg > 2:438320/1‑141 (MQ=255) tttGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGcc < 2:287920/141‑1 (MQ=255) ttGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCGTACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAGCAGGTGCAAAGTCAATCCACATTGAATAAGCTACAGGGCAGCCAGTGGTAAAGCCa > 1:610288/1‑141 (MQ=255) gtgCATTGTTAAAACATTTGTACCATCTTCTAGGTCATACTCATAACGAATGACTTCACGAGATAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATCAAGCCAcg > 1:265532/1‑141 (MQ=255) tgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTGAATCCCCATTGAATAAGATCCAGGACAGCCAATGATAAAGCAAcca > 2:155358/1‑139 (MQ=255) tgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCAcgc > 2:316877/1‑141 (MQ=255) gCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATCATAAAGCCAcgcg > 2:481221/1‑141 (MQ=255) gCATTGCTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTACAAAGTGACTCCCCAGTGGATAAGCTACCGGACAGCAAGTGATACAGCTCcgcg > 2:602987/1‑141 (MQ=255) ttGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCtt < 2:622260/141‑1 (MQ=255) ttGTTAAAACATTTGCACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCtt < 2:622322/141‑1 (MQ=255) ttAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAg < 1:549823/141‑1 (MQ=255) aaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTcc < 1:556910/141‑1 (MQ=255) gTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACg > 1:308024/1‑141 (MQ=255) tCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACa < 2:181864/141‑1 (MQ=255) tCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACa < 1:355301/141‑1 (MQ=255) cttgctCATACTCATAACGAACGACTTCACGCGTTATTTTCCCTTCTTTTCTAACAGGTGCGAAGTCAATCCCCATTGAAGAAGCTACAGGACAGCCAATGATATAGCCTCGCGCTTCAGGTCCTTCAACGATAGCAACAt < 2:175527/136‑1 (MQ=255) ctcggTCATGCTCATAACGAATGACTTCACGATGTCATTTCCCTTCTTTGCTAACAGGTGCAAAGTCAATCCCCTTTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAt < 2:610288/138‑1 (MQ=255) cTAGGTCATCCTCCTAACGAATGCCTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAt < 1:523829/141‑1 (MQ=255) tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTg < 1:94210/141‑1 (MQ=255) tCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATACCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACACGCTTCAGGTCCTACAACGATATCAACAGCTCTGACtttt > 2:514026/1‑141 (MQ=255) tCATAACGAATGACTGCACGAGGTACTTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCAGTGACTAAGCTACAGGACAGCCAATGATAAAGCCCCGCGCTTCAGATCCTACAACGATATCAAccactcgacctttt > 2:2692/1‑128 (MQ=255) cATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTAAATCCCAATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTg > 2:238601/1‑141 (MQ=255) aTAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTATCAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGc < 2:341285/141‑1 (MQ=255) aaCGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACATGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGt > 1:459580/1‑141 (MQ=255) cGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAt < 2:487948/141‑1 (MQ=255) tGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTa < 1:303898/141‑1 (MQ=255) gccTTAACGAGGTAATTTCCCTTCTTTTCAAACAGAGGCAAAGTCAATCCTCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTAc < 2:459580/139‑1 (MQ=255) aCTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACa > 2:450694/1‑141 (MQ=255) cACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGCATACGCACCAGGCCAGTCATTGATAAAGCCACTGTCTTTACGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAttt < 1:296954/141‑1 (MQ=255) cACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAttt < 1:592389/141‑1 (MQ=255) gAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCGACGCGCTTCAGGTCCTACAAGGATATAAACATCTTTGTGTTTTGCGTAGTCTACAATTTTAt > 1:287850/1‑141 (MQ=255) gAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCCCTGTCTTTTGCGTATTCTACAATTTTAt > 2:102594/1‑141 (MQ=255) gAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCACTTCCTACAACGATATCAACATCCCCGTCTATGCCGCACGCCACAATTTTAt > 1:138446/1‑141 (MQ=255) tgTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAGCTCTGTCTTTTGCGTATTCTACAATTTTATCt < 1:260447/140‑1 (MQ=255) ggTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAACCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTCGCGTATTATACAATTCGATCt > 1:33719/1‑141 (MQ=255) ggTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATACCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTACTACAACGATATCAACATCTCTGTCTTTTGCGTGTTCTACAATTCTATCt > 2:623429/1‑141 (MQ=255) gTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTTTTTTTTACTTGTTATATCATTTTATATg > 1:2176/1‑141 (MQ=255) gTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCtt > 1:447023/1‑140 (MQ=255) tAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGt < 1:438320/141‑1 (MQ=255) tttCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGTATAAACTACCGGACAGCCAGTGATAAAGCTGCCCGCAACAAGGAATGCAACGTAAACAACTCCGCGGTCTTTCGCGTATTCTCCAATTTTTTCTGtttc > 2:140173/1‑139 (MQ=255) tCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAACGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTATACAATTTTATCTGTTGCATAGCCATATGc > 2:491555/1‑141 (MQ=255) cAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACACCGACATCAACATCTCCGTCCTTCGCGTCTTCTCCAATCTTATCTGTTGCACAGCCATATGCTTCAc < 2:490459/141‑1 (MQ=255) cAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAc < 1:537219/141‑1 (MQ=255) gCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTGTATGCAACATAGCCATATGCTTCACCATTa < 2:308024/141‑1 (MQ=255) | TCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTA > CP000730/1744319‑1744588 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |