Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,760,411 G→T 11.8% S127* (TCA→TAA)  radC ← DNA repair protein RadC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,760,4110GT11.8% 165.3 / 4.0 73S127* (TCA→TAA) radCDNA repair protein RadC
Reads supporting (aligned to +/- strand):  ref base G (28/32);  new base T (8/2);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 8.54e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGATGGATGATTATGAACTGCGATGATTGCATTGGCATTTTCTCTCACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTCCCCATCCTTTCCCCTAACTCAAA  >  CP000730/1760273‑1760550
                                                                                                                                          |                                                                                                                                           
ccGGATGGATGATTATGAACTGCGATGATTGCATTGGCATTTTCTCTCACCGCAATACTAAAAATTTCAAGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGaa                                                                                                                                           >  2:624639/1‑141 (MQ=255)
    atggatgATTATGAACTGCGATGATTGCATTGGCATTTTCTCTCACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATTTACCTTTCAAAACACATGTTTCTTTAATCACTACATTTTTTTCATTTa                                                                                                                                       >  2:613544/1‑141 (MQ=255)
    aaggatgTTGATTAACTGCGAGGATTGCATTGGCATTTTCTCTCACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTa                                                                                                                                       >  2:558990/3‑141 (MQ=255)
     tggatgATTATGAACTGCGATGATTGCATTGGCATTTTCTCTCACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTaa                                                                                                                                      >  1:308161/1‑141 (MQ=255)
     tggatgATTATGAACTGCGATGATTGCATTGGCATTTTCTCTCACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTaa                                                                                                                                      <  1:632165/141‑1 (MQ=255)
      ggatgATTATGAACTGCGATGATTGCATTGGCATTTTCTCTCACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAAc                                                                                                                                     >  1:534114/1‑141 (MQ=255)
         tgatTATGAACTGCGATGATTGCATTGGCATTTTCTCTCACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAAt                                                                                                                                  <  1:332166/141‑1 (MQ=255)
          gatTATGAACTGCGATGATTGCATTGGCATTTTCTCTCACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATa                                                                                                                                 >  2:125503/1‑141 (MQ=255)
                gAACTGCGATGATTGCATTGGCATTTTCTCTCACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGa                                                                                                                           >  1:166725/1‑141 (MQ=255)
                  aCTGCGATGATTGCATTGGCATTTTCTCTCACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACa                                                                                                                         <  2:24371/141‑1 (MQ=255)
                   cTGCGATGATTGCATTGGCATTTTCTCTCACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTTTTTTTTTCTATAAAATTACaa                                                                                                                        >  2:20654/1‑141 (MQ=255)
                   cTGCGATGATTGCATTGGCATTTTCTCTCACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACaa                                                                                                                        <  1:399952/141‑1 (MQ=255)
                          gatTGCATTGGCATTTTCTCTCACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTCTAGAAAGCACATAGTTGGAATTTAACAATAAAATGACAAAATGTTc                                                                                                                 >  1:347311/1‑141 (MQ=255)
                              tcATTGGCATTTTCTCTCACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTtgt                                                                                                             <  2:654476/140‑1 (MQ=255)
                                      aTTTTCTCTCACCGCAATACTAAAAATTTCACGTGGATGTAACATCGAACTATTTAATGTACCTTTAGAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAACATAACAAAACGTTCTTCTTTTAAATc                                                                                                     >  2:14533/1‑141 (MQ=255)
                                       ttttCTCTCACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCt                                                                                                    <  2:261380/141‑1 (MQ=255)
                                        tttCTCTCACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTGTTGAATTTAACAATAAAATGACAAACTGTTCTTGTGTTAACTCtt                                                                                                   >  2:570277/1‑141 (MQ=255)
                                        tttCTCTAACCGCAATACTAAAAATTTCGCGTGGATGAACAATCGCACAATTTAATGTACCTTTAAAAACACCGGTTTCTTTAATCACTGCATTTTTTGAATTTACCAATGAAATAACAAAATGTTGTTGAGTGAAATCtt                                                                                                   >  2:422755/1‑141 (MQ=255)
                                         ttCTCTCTCCGTAGTATTATATATTTCACGTGTATGTACAATCGAGCTATTTAATGGACCTTTAAAAACACAGGTTTCTTTATTCAGTTCATGGTGTTAATTTAACAATAAAATGACAAAATGGTCTTGTGTTAAATCttt                                                                                                  <  2:976/141‑1 (MQ=255)
                                         ttCTCTCACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACACTAAAATGACAAAATGTTCTTGTGTTAAATCttt                                                                                                  >  2:26128/1‑141 (MQ=255)
                                          ttcctcACCGCAATACTAAAAATTTCACGTGGATGGACCATCTAACTATTTAATGTTCCTTTAAAAACACACGTCTCTTTAATCACTACATTTTTTGAATTTAATCATAAAATGACAAAATGTTCTTGTGTTAAATCTTTc                                                                                                 <  2:25311/138‑1 (MQ=255)
                                          tctctcACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGCATTTAACAATAACATGACAAAATGTTCTTAGTTTAAAACTTTc                                                                                                 >  2:77694/1‑141 (MQ=255)
                                          tctctcACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTc                                                                                                 >  2:199799/1‑141 (MQ=255)
                                           ctctcACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTTTTTTTTTTATTAAAATTACAAAATGTTCTTGTTTTATATGTTTct                                                                                                >  2:245651/1‑140 (MQ=255)
                                            tctcACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCAt                                                                                               <  1:617005/141‑1 (MQ=255)
                                             ctcACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCAtt                                                                                              <  2:341568/141‑1 (MQ=255)
                                             ctaaCCGCAATACTAATAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCAtt                                                                                              <  2:41016/138‑1 (MQ=255)
                                              tcACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCAttg                                                                                             >  1:598943/1‑141 (MQ=255)
                                                     aaTACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAAGAATAAAATGACAAAATGTTCTTGTGTTAAAACTTTCATTGTTGGaaa                                                                                      >  2:150950/1‑140 (MQ=255)
                                                       tACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACACTAAAGTGACAAGAGGTTCTTGTGTTAAATCTTTCATTGTTGGAATCa                                                                                    >  1:347589/1‑141 (MQ=255)
                                                        aCTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTAAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCat                                                                                   <  1:569345/141‑1 (MQ=255)
                                                         cTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTCCATTTTTGGAATCata                                                                                  >  2:8916/1‑141 (MQ=255)
                                                                  tCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCa                                                                         <  1:375204/141‑1 (MQ=255)
                                                                    aCGTGGATGTACATTCGTACTATTTAATGTTACTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCatattataatcggt                                                                       <  2:162399/141‑11 (MQ=255)
                                                                    aCGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATGATCCGCAAc                                                                       >  1:561228/1‑141 (MQ=255)
                                                                    aCGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAAc                                                                       >  1:338523/1‑141 (MQ=255)
                                                                      gTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTTTTTTTTTTTATAAAATGACAAAATGTTCTTGTGTTATATCTTTCATTGTTGGAATCATATTATCATCAACAt                                                                     >  2:427591/1‑141 (MQ=255)
                                                                       tGGATGTACAATCGAACTATTTAATGTACCTTTAAAACCAAAGGTTTCTTTACTCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAACCTTTCATTGTTGGAATCATATAATCAGCAACATc                                                                    <  1:56814/141‑1 (MQ=255)
                                                                           tggaCAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACtt                                                                <  1:132009/138‑1 (MQ=255)
                                                                           tGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCTCTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCCTAGTTGGAATCATATAATCAGCAACATCACtt                                                                <  2:442164/141‑1 (MQ=255)
                                                                                      aCTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTAt                                                     >  1:152177/1‑141 (MQ=255)
                                                                                        tATTTAATGGACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTAttt                                                   <  2:216914/141‑1 (MQ=255)
                                                                                           ttAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTTTTTTTTTTTATAAAATGACACGACGTTCTTGTGTTATATCTTTCATTGTGGGACTCATATAATCAGCAACATCACTTGGTTACGTTATATTta                                                >  2:663856/1‑141 (MQ=255)
                                                                                            tAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTtat                                               >  1:395493/1‑141 (MQ=255)
                                                                                              aTGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATAc                                             >  1:196731/1‑141 (MQ=255)
                                                                                               tGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTACCAATAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCCGCAACATCACTTGGTTGCGTTATTTTTATACgc                                           >  1:175038/1‑140 (MQ=255)
                                                                                               tGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACg                                            <  1:573322/141‑1 (MQ=255)
                                                                                                    cTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGattat                                       <  2:550716/141‑1 (MQ=255)
                                                                                                      ttAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTAttt                                     <  1:581554/141‑1 (MQ=255)
                                                                                                       tAAAAACACAGGTTTCTTTAATCACTACATTTTTTTTTTTTTATTATAAGATGAAAAAATGTTCTTGTTTTAAATAGTTCGTTGTTGGAATCAAATGATCAGCACCATAACTTGGTTGCGTTATTTTTCTAAGatttcttt                                    >  2:331741/1‑136 (MQ=255)
                                                                                                            ccacaGGTTTCTTTAATCCCTACATTTTTTGAATTTAACAATAAAACGACAAAAGGGTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGct                               <  1:253999/140‑1 (MQ=255)
                                                                                                                    ttCTTTAATCACTACATTTTTTTTTTTTTTTTTTAAAATAACAAAATGTTCTTGTTTTATTTCTTTCATTGTTGGAATCATATGTTCAGTAACATCCCTTAGTTGCGTTATTTTTATACGATTAttttttgttctttttct                       >  2:592723/1‑128 (MQ=255)
                                                                                                                     tCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTcccc                      >  1:626639/1‑141 (MQ=255)
                                                                                                                      cTTTAATCACTACATTTTTTTTTTTTTTTTTTAAAATGAAAAAATGTTCTTGTGTTTAATCTTTCATTGTTGGTATCATATGATCATCAACATCACTTGGTTTCGTTATTTTTATACGATTATTTTCTGCTCTTCTtccct                     >  2:36061/1‑140 (MQ=255)
                                                                                                                      cTTTAATCACTACATTTTTTGAATTTAACAATAAAATGTCAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGGAACATTCCTTGGTTGAGTTATTTTTATACGATTATTTTCATCTTTTCTCACCa                     >  2:153857/1‑141 (MQ=255)
                                                                                                                      cTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTCCCCa                     >  1:456030/1‑141 (MQ=255)
                                                                                                                       tttAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTCCCCAt                    >  1:334946/1‑141 (MQ=255)
                                                                                                                       tttAATCACTACACTTTTTGGATTTAACAATAAAATGACAAACTGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTCCCCAt                    <  1:79389/141‑1 (MQ=255)
                                                                                                                       cttAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTACTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTCCCCAt                    <  2:313390/140‑1 (MQ=255)
                                                                                                                        ttAATCACTTCATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTCCCCATc                   <  2:285489/141‑1 (MQ=255)
                                                                                                                          aaTCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATAAGATAATGTTTTTCTCTTCTCCCCATCCt                 <  2:308161/141‑1 (MQ=255)
                                                                                                                           aTCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTCCCCATCCtt                <  1:401335/141‑1 (MQ=255)
                                                                                                                            ttaCTACATTTTCTAAATTTAACAATAAAATGACAAAATTTTCTTTTGTTAAATCTTTCATTGTTGTAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTCCCCATCCttt               <  2:386915/139‑1 (MQ=255)
                                                                                                                             cACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTCCCCATCCTTTc              <  2:48446/141‑1 (MQ=255)
                                                                                                                             cACTACATTTTTTAAATTTAACAATAAAATGACAAAATGTTCTTTTGTTAAATCTTTCATTGTTGGTATCATATAATCAGCAACATCTCTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTCCCCATCCTTTc              <  2:318161/141‑1 (MQ=255)
                                                                                                                              aCTACATTTTTTGAATTTAACAACAAAAGGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTCCCCATCCTTTcc             <  1:8916/141‑1 (MQ=255)
                                                                                                                                    ttttttTAATTTAGAAATAAAATGACAAAATGTTCTTGTTTTCAATCTTTCATTGTTGGAATCATATAATCTGCAACCTCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTCCTCCCCATCCTTTCCCCTatc       <  2:324734/141‑3 (MQ=255)
                                                                                                                                    ttttttGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTCCCCATCCTTTCCCCTAAc       <  2:324661/141‑1 (MQ=255)
                                                                                                                                    ttttttGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTCCCCATCCTTTCCCCTAAc       >  1:519263/1‑141 (MQ=255)
                                                                                                                                     tttttGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTCCCCATCCTTTCCCCTAACt      <  1:416249/141‑1 (MQ=255)
                                                                                                                                     tttttGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTCCCCATCCTTTCCCCTAACt      <  2:347311/141‑1 (MQ=255)
                                                                                                                                      ttttGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTCCCCATCCTTTCCCCTAACTc     <  2:572030/141‑1 (MQ=255)
                                                                                                                                         tGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTCCCCATCCTTTCCCCTAACTCaaa  <  2:347589/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CCGGATGGATGATTATGAACTGCGATGATTGCATTGGCATTTTCTCTCACCGCAATACTAAAAATTTCACGTGGATGTACAATCGAACTATTTAATGTACCTTTAAAAACACAGGTTTCTTTAATCACTACATTTTTTGAATTTAACAATAAAATGACAAAATGTTCTTGTGTTAAATCTTTCATTGTTGGAATCATATAATCAGCAACATCACTTGGTTGCGTTATTTTTATACGATTATTTTCAGCTCTTCTCCCCATCCTTTCCCCTAACTCAAA  >  CP000730/1760273‑1760550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: