BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,812	G→A	42.2%	intergenic (‑895/‑273)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,812	0	G	A	42.2%	51.4 / 109.9	100	intergenic (‑895/‑273)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (27/30); new base A (26/16); total (53/47)
Fisher's exact test for biased strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

Only 100 of 110 total aligned reads displayed.

CGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACG  >  CP000730/1929673‑1929949

cGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagt                                                                                                                                                >  2:229980/1‑141 (MQ=255)

  ccTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagtag                                                                                                                                              >  2:438189/1‑141 (MQ=255)

  ccTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagtag                                                                                                                                              >  1:619904/1‑141 (MQ=255)

          tttAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCt                                                                                                                                      <  1:377162/140‑1 (MQ=255)

          cTTAAATTAACACTATCTATGCTCCTAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTCTCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCt                                                                                                                                      <  1:508562/141‑1 (MQ=255)

          cTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGGGCCGCCt                                                                                                                                      >  2:425465/1‑141 (MQ=255)

          cTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCt                                                                                                                                      <  1:410201/141‑1 (MQ=255)

           ttAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTGGCCTa                                                                                                                                     >  1:394030/1‑141 (MQ=255)

                  aaCAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCa                                                                                                                              <  2:550975/141‑1 (MQ=255)

                    cAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAgg                                                                                                                            <  1:266783/141‑1 (MQ=255)

                       tatCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGt                                                                                                                         >  1:7212/1‑141 (MQ=255)

                        atCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTc                                                                                                                        >  1:198947/1‑141 (MQ=255)

                         tCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAATGGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCa                                                                                                                       <  1:91965/141‑1 (MQ=255)

                         tCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCa                                                                                                                       <  1:264123/141‑1 (MQ=255)

                         tCTATTCTCAGAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCa                                                                                                                       <  1:524843/141‑1 (MQ=255)

                            aTTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAAtt                                                                                                                    >  2:51669/1‑141 (MQ=255)

                                    aGAATTTGTCCAATTAAGTGTAGCCGATTCAATCTCTCAGCTAGAATCTTATGACTGGTCAGAAGCAATTGACTGGTCTTGTAGATTTGCGGATGGTATAATAGTAGAGGCGCCTATCTCTCAGGCGTCAATTTAGACGCa                                                                                                            <  2:223840/141‑1 (MQ=255)

                                    aGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCa                                                                                                            <  2:120252/141‑1 (MQ=255)

                                       aTTTGTCCAATTAAGTGTAGCCGGTTCCTTCAACTGCCTAGAGTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGGAGAGTCGGCTAGCTCTCTGGCGTCAATTTAGACGCagag                                                                                                         <  2:302490/141‑1 (MQ=255)

                                         ttGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagagag                                                                                                       >  2:52323/1‑141 (MQ=255)

                                         ttGGCCAATGAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTTCTTGTATATGCGCCGAGGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagagag                                                                                                       <  2:198903/141‑1 (MQ=255)

                                           gTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGCAGCAAGTGACTGTGCTTGTATATTTGCCGATGATAAGATAGTAGAGTCGCCTATCTCTCAGGCGACAATTTGGACGCAGAgaggg                                                                                                     >  1:262162/1‑140 (MQ=255)

                                              cAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGgt                                                                                                  <  2:359265/141‑1 (MQ=255)

                                              cAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAAGTTAGACGCGGAGGGGAGgt                                                                                                  >  1:518861/1‑141 (MQ=255)

                                                   aaGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGta‑ta                                                                                            <  1:385609/141‑1 (MQ=255)

                                                   aaGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGta‑ta                                                                                            <  2:334283/141‑1 (MQ=255)

                                                     gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑g                                                                                         <  2:104716/141‑1 (MQ=255)

                                                     gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGGGTA‑TAA‑g                                                                                         >  1:33137/1‑141 (MQ=255)

                                                     gtggAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑g                                                                                         <  2:469274/141‑1 (MQ=255)

                                                                  aaTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAttt                                                                        <  2:55256/141‑1 (MQ=255)

                                                                   aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGGGGAGGTGTA‑TAA‑G‑G‑G‑GGTGC‑TTGtttt                                                                       >  1:67697/1‑141 (MQ=255)

                                                                   aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAtttt                                                                       >  1:617662/1‑141 (MQ=255)

                                                                                aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATa                                                          <  1:40282/141‑1 (MQ=255)

                                                                                aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATa                                                          <  1:40471/141‑1 (MQ=255)

                                                                                 aTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAg                                                         >  1:104477/1‑141 (MQ=255)

                                                                                 aTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAg                                                         >  2:651616/1‑141 (MQ=255)

                                                                                      ataACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCAATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCa                                                    >  1:456894/1‑141 (MQ=255)

                                                                                         aCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCTGtc                                                 >  2:546668/1‑141 (MQ=255)

                                                                                               cAGAAGCAAGTGACTGTGCTTGTTCTTGAACCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGt                                           >  1:661663/1‑141 (MQ=255)

                                                                                               cAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGt                                           >  2:655242/1‑141 (MQ=255)

                                                                                                aGAAGCAAGTGACTGTGTTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTg                                          <  1:501325/141‑1 (MQ=255)

                                                                                                aGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTGGTTCACATCATAGCACCAGGAATCAGTg                                          >  1:255828/1‑141 (MQ=255)

                                                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                        >  1:621917/15‑141 (MQ=255)

                                                                                                  tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                       >  1:443158/14‑141 (MQ=255)

                                                                                                    ggaagtaggtatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                     <  1:82708/130‑1 (MQ=255)

                                                                                                    ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                     >  1:168329/12‑141 (MQ=255)

                                                                                                    ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTT‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTAATAAGTGGCtgt                                     >  1:368623/12‑141 (MQ=255)

                                                                                                    gCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACGTAATAGCAGCAGTCATCAGTGGCtg                                      >  2:189474/1‑141 (MQ=255)

                                                                                                    gCAAGTGACTGTGCTTGTATATTTACCGATGATATAATAGTAGAGTCGCCTATCTCGCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                      >  2:490596/1‑141 (MQ=255)

                                                                                                      aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                   <  1:442291/132‑1 (MQ=255)

                                                                                                      aaGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                    >  1:291877/1‑141 (MQ=255)

                                                                                                       agtagatattcTTCTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                  <  2:500018/131‑2 (MQ=255)

                                                                                                       agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                  <  2:618990/133‑2 (MQ=255)

                                                                                                       agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTGTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                  <  1:430107/133‑2 (MQ=255)

                                                                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                 <  1:543834/134‑3 (MQ=255)

                                                                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                 >  1:45839/8‑139 (MQ=255)

                                                                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGGCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                 <  1:170476/134‑3 (MQ=255)

                                                                                                        gcagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                 >  2:578301/8‑139 (MQ=255)

                                                                                                          gACTGTGCTTGTATATTTGCCGATCATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCCCATCATAGCACCAGTCAGCAGTGGCTGTGCCAt                                <  1:134979/141‑1 (MQ=255)

                                                                                                          agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgct                               >  1:476726/6‑137 (MQ=255)

                                                                                                            atatgagtGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                             <  1:71702/134‑7 (MQ=255)

                                                                                                            atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                             <  1:603429/138‑7 (MQ=255)

                                                                                                             tatgattGTATATTCACAGATGGTATAATAATAGAATCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑T‑T‑GGAGCATCATTGTCGGTCACAGCATAGCCCCAGTCATCAGTGGCCGTGCCGGTGCg                            >  2:41985/6‑141 (MQ=255)

                                                                                                             tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCGGTGGCTGTGCTGTTGCg                            >  1:117086/3‑141 (MQ=255)

                                                                                                              ttgCTTGTATAGTTGCCGATGATATAATAGTAGCGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCg                            <  2:101255/140‑1 (MQ=255)

                                                                                                              gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCg                            >  1:416956/1‑141 (MQ=255)

                                                                                                              atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                           <  2:516675/140‑1 (MQ=255)

                                                                                                               tgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGta                          >  1:32426/1‑140 (MQ=255)

                                                                                                                 cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                        <  1:557506/141‑4 (MQ=255)

                                                                                                                  ttGTATATTCACAGATGGGATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCCGTCATCAGTGGCTGTGCTGTTGCGtatta                       <  1:594485/141‑2 (MQ=255)

                                                                                                                    gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATAATAGCACCAGTCATCAGTGGCCGTGCTGTTGCGtattata                     >  2:374439/1‑138 (MQ=255)

                                                                                                                     tatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtgttatac                    >  1:187644/11‑137 (MQ=255)

                                                                                                                     tatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatat                    >  2:605751/11‑137 (MQ=255)

                                                                                                                     tatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑T‑TGGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                    >  1:451585/11‑137 (MQ=255)

                                                                                                                     tatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGACGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TAATTTTCGTTCACATCATAGCACCAGGCATCAGTGGCTGTGCTGTTGCGtattatac                    >  2:605744/11‑137 (MQ=255)

                                                                                                                     tataTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATTC‑TTATCTTCGTTCGCATCATAGCACCAGTCATCAGTGCCTGTGCCATTGCGttttttt                     <  1:407313/141‑1 (MQ=255)

                                                                                                                      atattcacagatggtATAATAATAGAGTCGCGTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGAGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTACACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatact                   >  1:491760/10‑136 (MQ=255)

                                                                                                                       tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACtt                  >  1:303874/9‑141 (MQ=255)

                                                                                                                       aatTTGCCGATGATATAATAGGAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCt                   <  2:262162/140‑1 (MQ=255)

                                                                                                                        tatcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                 >  2:337992/8‑141 (MQ=255)

                                                                                                                        attcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                 >  2:121783/8‑141 (MQ=255)

                                                                                                                        atTTGCCGATGATATAATAGTAGAGTCGACTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCtt                  <  1:463886/141‑1 (MQ=255)

                                                                                                                            acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGCGGAGGTGCATTAC‑T‑T‑T‑GCTAG‑TCCTTGTCGCTCACATCAGAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg             >  2:640275/4‑141 (MQ=255)

                                                                                                                            acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg             <  1:252100/138‑1 (MQ=255)

                                                                                                                             cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc            <  2:591541/139‑1 (MQ=255)

                                                                                                                             cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc            >  1:9688/3‑141 (MQ=255)

                                                                                                                              cGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTgtt            >  2:606001/1‑139 (MQ=255)

                                                                                                                              cGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc            <  2:40546/141‑1 (MQ=255)

                                                                                                                                 tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg        >  1:183012/4‑141 (MQ=255)

                                                                                                                                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATTATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt       <  2:20863/139‑1 (MQ=255)

                                                                                                                                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTCCGTATTGTACTTCTTGGCTTAgg       >  2:2184/3‑140 (MQ=255)

                                                                                                                                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt       >  2:621136/3‑141 (MQ=255)

                                                                                                                                   gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGCTCACCTCAAAACACCTGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa      >  1:126114/2‑141 (MQ=255)

                                                                                                                                   atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGt       >  2:647888/1‑141 (MQ=255)

                                                                                                                                     ctaataaGAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa    <  1:533308/140‑3 (MQ=255)

                                                                                                                                     ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa    >  1:328466/1‑139 (MQ=255)

                                                                                                                                     ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa    >  1:145633/1‑139 (MQ=255)

                                                                                                                                      taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑GCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATAATTATTGGCttggtaaac   >  1:91844/1‑133 (MQ=255)

                                                                                                                                       aataatACAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg  <  2:430551/141‑1 (MQ=255)

                                                                                                                                        ataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTacctcgacc  >  2:429641/1‑133 (MQ=255)

CGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACG  >  CP000730/1929673‑1929949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`