BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,940	G→A	27.3%	R204R (CGC→CGT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,940	0	G	A	27.3%	448.8 / 200.2	342	R204R (CGC→CGT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (115/130); new base A (46/47); total (162/180)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

Only 100 of 352 total aligned reads displayed.

TGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT  >  CP000730/35799‑36080

tattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                                             <  1:6332/136‑1 (MQ=11)

 atttaaataaAAAT‑GATGGTTCTTTTGCAAAGT‑AAAAAATAAAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                                                                                            <  1:34827/136‑1 (MQ=11)

  ttttaataaAAAT‑GATTGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGCTCTGAAGAGACCTGCgg                                                                                                                                           >  1:332441/4‑141 (MQ=11)

  ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATAGAGCTAAACACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                                           >  2:433434/4‑141 (MQ=11)

   tCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                                                                                            <  1:260334/141‑1 (MQ=255)

    ttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                                                                                         <  2:19666/140‑1 (MQ=11)

     taataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGttt                                                                                                                                        >  2:295852/1‑140 (MQ=11)

      aataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                                                                                         <  1:486613/141‑1 (MQ=39)

      aataaAAAT‑GATGGTTCTGTAGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTTGTTTAAATAACAA‑CAAAATGGCTAATTTCGTGGCATGGCGAAAATCCGTAGGACTGAAGAGAGCTGCGGATCt                                                                                                                                       >  1:154361/1‑141 (MQ=2)

                gcgtgtgcTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGGTCGTATAACTTGCTAGCATGAT‑GCTAATTTCGTTGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTtatata                                                                                                                              <  2:167492/134‑1 (MQ=1)

                                                 aatcACTAATATATCATGTCAGTTTTCGTTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGATGTTTTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTa                                                                                              <  1:352877/138‑1 (MQ=1)

                                                      ctcaTTTATCGTGACAGTGTTCGCTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGTGCGTAAATACATTCAATACCTTTTAAAGTATTCtt                                                                                         <  1:393780/138‑1 (MQ=0)

                                                                    cAGTGTTCGCTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                           <  2:618958/141‑1 (MQ=11)

                                                                     aGTGTTCGTTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt                                                                          >  2:265416/1‑141 (MQ=11)

                                                                     aGTGTTCGTTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTCTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGACGTATTGATACt                                                                          >  2:384544/1‑141 (MQ=11)

                                                                     aGTGTTCGTTTAACTTGCTAGCATGAT‑GCAAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATGCCTTTTAAAGTATTCTTGGCCGTATTGATACt                                                                          >  2:123859/1‑141 (MQ=11)

                                                                     aGTGTTCGCTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                                                          >  2:444909/1‑141 (MQ=31)

                                                                     aGTGTTCGCTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGGATTCTTTGCTGTATTGATACt                                                                          >  1:286614/1‑141 (MQ=14)

                                                                      gttttCGTTTAACTTGCTAGCATGAT‑GCTTATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                         <  2:137669/138‑1 (MQ=255)

                                                                      gtgtTCGTTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGGGCAAGTCCTGAGGTCTGAAGAAGCCTACGGTTCTTCTTATATACAGCGTAAATACATTCAATACATTTTAAATTATTCTTTGCCGTATTGATACtt                                                                         >  2:109178/1‑141 (MQ=11)

                                                                      gtgtTCGTTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                         >  2:48420/1‑141 (MQ=255)

                                                                      gtgtTCGTTTAAATTGCTAGCATGAT‑GCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                         <  2:209692/141‑1 (MQ=11)

                                                                      gtgtTCGCTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                         >  1:505803/1‑141 (MQ=17)

                                                                       tgtTCGTTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                                                        >  2:545767/1‑141 (MQ=255)

                                                                       tgtTCGTTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGGAAATGCACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                                                        >  2:331683/1‑141 (MQ=255)

                                                                            gtttAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGCATCAAGCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                   <  1:655414/139‑1 (MQ=11)

                                                                             tttAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                                                  <  1:619467/140‑3 (MQ=255)

                                                                             tttAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                                                  <  1:236422/140‑3 (MQ=255)

                                                                             tttAACATGCTAGCATGAT‑GCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                                                  >  2:369623/2‑139 (MQ=11)

                                                                              ttAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                 <  2:498041/141‑1 (MQ=17)

                                                                              ttAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                 <  2:346963/141‑1 (MQ=17)

                                                                               tAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                                >  1:591403/1‑141 (MQ=17)

                                                                                aaCTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                                               <  1:530244/141‑1 (MQ=32)

                                                                                 aCTTGCTAGCATGAT‑GCTAATTTCGTGGCAGGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGt                                                              <  1:128641/141‑1 (MQ=11)

                                                                                  cTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                             <  1:168067/141‑1 (MQ=32)

                                                                                      cTAGCATGCT‑GCTAATTTCGTGGCATGGCGAAATTCCGGAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                         <  2:505002/141‑1 (MQ=12)

                                                                                      cTAGCATGAT‑GCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttc                                                         <  1:364451/141‑1 (MQ=11)

                                                                                      cTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCGATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                         <  1:427960/141‑1 (MQ=17)

                                                                                       tAGCATGAT‑GCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                                                        <  1:420860/141‑1 (MQ=11)

                                                                                         gCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                      >  1:278941/1‑141 (MQ=17)

                                                                                         gCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                      >  1:245874/1‑141 (MQ=17)

                                                                                         gCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                      <  1:252161/141‑1 (MQ=17)

                                                                                           atgat‑gCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACt                                                    >  1:268979/1‑141 (MQ=255)

                                                                                            tgat‑gCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                                                   >  1:553705/1‑141 (MQ=255)

                                                                                            tgat‑gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACtt                                                   >  2:514385/1‑141 (MQ=21)

                                                                                            tgat‑gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACtt                                                   >  1:98433/1‑141 (MQ=21)

                                                                                             gat‑gCTAATTTCGTGTGATGGCGAAAATGCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTGTGATACCTTGTCTTTCTTACttt                                                  <  2:59784/141‑1 (MQ=11)

                                                                                             gat‑gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                  >  1:638843/1‑141 (MQ=21)

                                                                                               t‑gCTAATTTCGGGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTaa                                                <  2:203730/141‑1 (MQ=25)

                                                                                                 gCTAATTTCGTGGCATGGGGAAAATCCGTAGATATGCACAAACTACATACTACTTGCGCAAGGAGCGTAAATCAATTCAATACCTTTTAAAGTATTTTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAat                                               <  2:304882/141‑1 (MQ=2)

                                                                                                   tAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAatat                                             >  2:36175/1‑141 (MQ=11)

                                                                                                   tAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAatat                                             >  1:210343/1‑141 (MQ=255)

                                                                                                   tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTTCTGTATTGATACTTTGATACATTGTCTTTATTGCGTTTatat                                             >  1:52350/1‑141 (MQ=11)

                                                                                                   tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                                             >  1:199582/1‑141 (MQ=21)

                                                                                                       ttCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg                                         >  2:668247/1‑141 (MQ=11)

                                                                                                         cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                       <  2:586383/141‑1 (MQ=38)

                                                                                                          gTGGCATGGCGAAAATCCGTAGATCTCAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                      <  1:359423/141‑1 (MQ=255)

                                                                                                           tGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATAACGACTCTCTTACTTTAATATGACGGTGa                                     <  1:324777/141‑1 (MQ=14)

                                                                                                           tGGCATGGCGAAAATCCGTCGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTTATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                     >  1:148313/1‑141 (MQ=255)

                                                                                                             gCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGGTACTTTGGTATCTTGTCTTTCTTACTTTAATATGACGGTGATc                                   >  2:56558/1‑141 (MQ=11)

                                                                                                              cATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTCATATGGCGGTGATCt                                  >  1:3768/1‑141 (MQ=255)

                                                                                                               aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGAGAGCtt                                 >  2:543198/1‑141 (MQ=255)

                                                                                                                tGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                <  1:500522/141‑1 (MQ=255)

                                                                                                                tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                <  2:401665/141‑1 (MQ=255)

                                                                                                                 ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                               >  2:286034/1‑141 (MQ=37)

                                                                                                                 ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                               <  1:541315/141‑1 (MQ=255)

                                                                                                                  gggAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATGCTTTGATATCTTGTCTTTCTTGCTTTAATATGACGGTGGTCTTGCt                              >  1:103932/3‑141 (MQ=11)

                                                                                                                  gCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                              >  2:218732/1‑141 (MQ=38)

                                                                                                                   cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc                             <  1:296196/141‑1 (MQ=255)

                                                                                                                    gAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAAGACCTTTTAAAGTATTCTTTGCTGTATTGATCCTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCa                            >  2:461413/1‑141 (MQ=255)

                                                                                                                    gAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTAAATACCTTTTAAAGTATACTTTGCCGTATTGATACTTTACTATCTTGTCTTTCTTAATGTCATATGACGCTAATCTTGccaa                            >  2:18742/1‑138 (MQ=11)

                                                                                                                      aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGccaaat                          >  1:178955/1‑136 (MQ=255)

                                                                                                                      aaaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTCCTCAAt                          >  2:53616/1‑141 (MQ=255)

                                                                                                                       aaTCCGTAGATCTGAAGAGACCTACGGTTCTTCTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTAATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                         <  1:445943/141‑1 (MQ=14)

                                                                                                                           cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTCATATGACGGTGATCTTGCTCAATGAGGt                     >  1:464130/1‑141 (MQ=255)

                                                                                                                           cGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACATTAATATGACGGTGATCTTGCTCAAttaatt                     >  1:108249/1‑136 (MQ=11)

                                                                                                                            gTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGtt                    <  1:263966/141‑1 (MQ=39)

                                                                                                                            gTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATGTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGtt                    >  2:651667/1‑141 (MQ=255)

                                                                                                                             tAGAGCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATCCTCTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                   >  1:455462/1‑141 (MQ=255)

                                                                                                                              aGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAt                  <  1:497300/141‑1 (MQ=255)

                                                                                                                                aTCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                <  2:56202/141‑1 (MQ=38)

                                                                                                                                 gcTGAAGAGACCTGCGGTTCTGTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTGTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCa               <  2:75754/140‑1 (MQ=255)

                                                                                                                                  cTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg              <  2:259566/141‑1 (MQ=38)

                                                                                                                                  cTGAACAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg              <  2:426997/141‑1 (MQ=255)

                                                                                                                                   tGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa             >  1:310964/1‑141 (MQ=255)

                                                                                                                                   agAAGAGACCTACTGTTCTTTTTATATAGAGCGTAAATACATTCAATACATTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGCCGGTGATCTTGCTCAATGAGGTTATCCAGa             <  2:406750/140‑1 (MQ=14)

                                                                                                                                      atagaCCTTCGGTTCTTTTTATATAGAGCGTAAATACTCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGatat          <  2:631063/139‑1 (MQ=255)

                                                                                                                                      agagACCTGCGGTTCTTTTTATAGAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGatat          >  2:477495/1‑141 (MQ=255)

                                                                                                                                      agagACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGatat          <  2:102586/141‑1 (MQ=255)

                                                                                                                                       gagaCCTTGGGTTTTTTTGATATAGAGCGTAAATACATTCAATATCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt         <  2:568771/141‑1 (MQ=11)

                                                                                                                                       gagaCCTGCGGTTCTTTTTATATATAGCGTAAATACACTCAATACCTTTTAAATTATTCTTTGCTGTATTGATACTTTGATACCGTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt         <  2:236850/141‑1 (MQ=255)

                                                                                                                                        agaCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAttt        >  2:246740/1‑141 (MQ=255)

                                                                                                                                         gaCCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGCGGTTATTCAGATATTTc       >  1:585560/1‑141 (MQ=38)

                                                                                                                                         gaCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTc       <  1:13669/141‑1 (MQ=255)

                                                                                                                                           ccTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa     <  1:612278/141‑1 (MQ=255)

                                                                                                                                            cTGCGTTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt    <  1:44277/141‑1 (MQ=255)

                                                                                                                                             tGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATg   >  1:404839/1‑141 (MQ=37)

                                                                                                                                             tGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATg   >  2:634511/1‑141 (MQ=255)

                                                                                                                                             tGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTCTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAGCTTGCTCAATGAGGTTATTCAGATATTTCGATg   >  2:373778/1‑141 (MQ=255)

                                                                                                                                              gCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCGATGAGGTTATTCAGATATTTCGATGt  >  1:75216/1‑141 (MQ=255)

TGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGAT‑GCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT  >  CP000730/35799‑36080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`