Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,021,817 C→T 8.3% V139I (GTT→ATT)  USA300HOU_1873 ← acyl‑CoA hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,021,8170CT 8.3% 203.3 / 6.2 74V139I (GTT→ATT) USA300HOU_1873acyl‑CoA hydrolase
Reads supporting (aligned to +/- strand):  ref base C (41/26);  new base T (3/3);  total (44/30)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

CCTTAACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACCACTT  >  CP000730/2021677‑2021954
                                                                                                                                            |                                                                                                                                         
ccTTAACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCCATTGTTTGTTTAATTTCCATGCGACGTGATTTTCTTCGTTCAACAAGTTGTGGTGCTGTATCATAAAACCATTTTTCAAc                                                                                                                                           >  1:300905/1‑141 (MQ=255)
ccTTAACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGTTGCTGTACCATAAAACCATTTTTCAAc                                                                                                                                           >  2:401374/1‑141 (MQ=255)
ccTTAACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAAc                                                                                                                                           >  2:130467/1‑141 (MQ=255)
 cTTAACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAAca                                                                                                                                          >  2:90510/1‑141 (MQ=255)
   tAACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAAcatc                                                                                                                                        <  2:516808/141‑1 (MQ=255)
    aaCAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAAcatca                                                                                                                                       <  1:225234/141‑1 (MQ=255)
    aaCAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAAcatca                                                                                                                                       >  1:568104/1‑141 (MQ=255)
       aaTCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATATAACCCTTTTTTAACATCAtct                                                                                                                                    >  2:22058/1‑141 (MQ=255)
           tGTCTTTATATTTTTAGTCCCTAATCTGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGCGCTTTTCTTCGTTCAACTCGTTGCGTTGCTGTAGCATAAAGCCACTTTTCAACATCAgcttctt                                                                                                                                >  2:483929/1‑140 (MQ=255)
                ttATATTTTTAGTCTCTAATATGTTGCACTTGGGATCCCCAGTCAATTGTTTGTTAACTGTGAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAGAACCATTTTTCAACATCATCTTCTGGatat                                                                                                                           >  1:658595/1‑141 (MQ=255)
                ttATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGatat                                                                                                                           >  2:146987/1‑141 (MQ=255)
                ttATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGatat                                                                                                                           <  2:633408/141‑1 (MQ=255)
                 tataTTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGAtata                                                                                                                          <  2:444181/141‑1 (MQ=255)
                      ttttCGTCTCTAATATGTTGCACTTGAGCTAAACATTCAATTGTTTGGTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCa                                                                                                                     <  2:202414/141‑1 (MQ=255)
                      ttttAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCa                                                                                                                     >  1:20160/1‑141 (MQ=255)
                      tttgAGTCTCCAATATGTTGCACTTGAGCTAAATATTCAATTGCTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTGCCACACGTTGTGGTGCTGGATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCa                                                                                                                     <  1:181140/141‑1 (MQ=255)
                       tttAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACAcccg                                                                                                                    >  1:227721/1‑139 (MQ=255)
                         tAGTCAATAATATGTTGCACTTGAGATCAATGTTCAATTGATTGTTTACTTTCAATGCGACGTGCTCTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCACCATCATCTTCTTGATATACACCAGGt                                                                                                                  >  1:490897/1‑141 (MQ=255)
                          aGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTa                                                                                                                 >  1:234633/1‑141 (MQ=255)
                           gTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTAc                                                                                                                >  2:378560/1‑141 (MQ=255)
                            tctcTAATATGTTGCACTTGAGCTAAATATTCACTTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACa                                                                                                               >  1:602797/1‑141 (MQ=255)
                              tctAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATg                                                                                                             <  1:146987/141‑1 (MQ=255)
                              tctAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATg                                                                                                             <  2:20160/141‑1 (MQ=255)
                                tAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGtt                                                                                                           <  1:566778/141‑1 (MQ=255)
                                tAATATGTTGCACTTGAGCTAAATACTCAATTGTTTGTTTACTTCCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGtt                                                                                                           <  1:166621/141‑1 (MQ=255)
                                     tGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGt                                                                                                      >  2:314511/1‑141 (MQ=255)
                                       ttGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGttt                                                                                                    <  2:258744/141‑1 (MQ=255)
                                           aCTTGAGCTAAATATTCCATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCt                                                                                                <  2:582202/141‑1 (MQ=255)
                                           aCTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCt                                                                                                >  1:523126/1‑141 (MQ=255)
                                               gAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCACCACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTtcat                                                                                            >  1:131965/1‑141 (MQ=255)
                                               gAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCATCATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTtcat                                                                                            <  1:10779/141‑1 (MQ=255)
                                               gAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTtcat                                                                                            <  1:111562/141‑1 (MQ=255)
                                                  cTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTcatcat                                                                                         >  1:34247/1‑141 (MQ=255)
                                                    aaaTATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCt                                                                                       <  1:119016/141‑1 (MQ=255)
                                                        atTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTaaag                                                                                   >  1:589731/1‑139 (MQ=255)
                                                        atTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACg                                                                                   <  2:286847/141‑1 (MQ=255)
                                                         ttCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGc                                                                                  >  1:492280/1‑141 (MQ=255)
                                                           cAATTGTTTGTCTACTTGCAGTGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAGCCATTTTTCGACATCATCTTCTGGGTATACACGAGGTACATGTTTAGGTTTGCCTTCATCATCTAACAcaa                                                                                >  2:6471/1‑141 (MQ=255)
                                                            agttttttgtttAGTTTGAATGCGACTGGCTTTTCTTCGTTCAAAAGTCTCGGGTGCTGTATCATAAAACCGTTGTTCAAGATCATCTTGTGGATATAAGCCATGTACATGTTTAGGTTTGCCGTCATCATCTAACGcaac                                                                               <  2:20719/136‑1 (MQ=255)
                                                            aaTTGTTTGTTTACTTTCAATGCTACGTGCTTTTTCTCTCTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGcaac                                                                               <  2:433733/141‑1 (MQ=255)
                                                             aTTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGcaaca                                                                              <  1:397746/141‑1 (MQ=255)
                                                              ttgtttgtTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGcaacaa                                                                             >  1:558403/1‑141 (MQ=255)
                                                              ttgtttgtTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGcaacaa                                                                             >  1:97010/1‑141 (MQ=255)
                                                                     tttACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTaa                                                                      >  1:212425/1‑141 (MQ=255)
                                                                        aCTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTa                                                                   >  1:359713/1‑141 (MQ=255)
                                                                        aCTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTa                                                                   >  2:22486/1‑141 (MQ=255)
                                                                          tttCAATGGGACGTGCTTTTCTTCGTTCAACCCGTTGTGGTGCTGGATCATAAAACCCTTTTTCAACATCATCTTCTGGATATACGCCAGGTACATGTTTAGGTTTGCCTTCATCATTTAACGCAACAAATGTTAAGTAAc                                                                 <  1:158864/141‑1 (MQ=255)
                                                                             cAATGCGACGTGCTTTGCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGCACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAAATTa                                                              >  1:371437/1‑141 (MQ=255)
                                                                                tGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATg                                                           <  1:40113/141‑1 (MQ=255)
                                                                                  cGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCCTCTAACGCACCAAATGTTAAGTAACTTAATgag                                                         >  2:251740/1‑139 (MQ=255)
                                                                                        gCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTaaa                                                   >  1:256931/1‑141 (MQ=255)
                                                                                         cTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAAt                                                  <  2:476030/141‑1 (MQ=255)
                                                                                         cTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAAt                                                  >  2:576667/1‑141 (MQ=255)
                                                                                         cTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAAt                                                  <  2:602797/141‑1 (MQ=255)
                                                                                          ttttCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCCTCTTCTGGATATACACCAGGTCCATGTTTAGGTTTGCCTTCATCATCTAACGCAACCAATGTTAAGTAACTTAATGCAGCTAAATc                                                 >  2:125497/1‑141 (MQ=255)
                                                                                                       acacGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACTAGGTACATGTTTAGGTTTGCCTTCAGCATCTAACGCAACAAATGTTAAGTAACTTAATGCATCTAAATCATGCTTGttatta                                    <  1:420938/141‑1 (MQ=255)
                                                                                                       acacGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGttatta                                    >  1:663167/1‑141 (MQ=255)
                                                                                                         acGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTaaa                                  >  1:116610/1‑141 (MQ=255)
                                                                                                            ttGTGGTGCTGACTCATAACACCATTTTTCAACATCATCTTCTGGATATACACCAGGTAGATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACATAATACAGCTAAATCATCCTAGTTATTAAATAc                               >  2:374104/1‑141 (MQ=255)
                                                                                                             tgtgGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATAATGCTTGTTATTAAATACa                              >  2:384480/1‑141 (MQ=255)
                                                                                                             tgtgGCGCTGTATCATAAAACCATTTTTCAACATCATCTTATGGATATACACCAGGTACATGTTTATGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAACTAAATCATGCTTGTTATTCAATaga                              >  1:490494/1‑139 (MQ=255)
                                                                                                              gtggtgCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACAt                             <  2:177564/141‑1 (MQ=255)
                                                                                                              gtggtgCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACAt                             <  2:177405/141‑1 (MQ=255)
                                                                                                               tggtgCTGTATCATAAAACCATTTTTCAACATCCTCTTCTGGATATACACAAACTACATGTCTATTTTTACCTTCATCATCTAACGCAACAAATGTGAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATc                            <  2:658595/141‑1 (MQ=255)
                                                                                                                  tgCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTc                         >  1:665589/1‑141 (MQ=255)
                                                                                                                  tgCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTc                         >  1:327739/1‑141 (MQ=255)
                                                                                                                  tgCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTc                         >  1:69986/1‑141 (MQ=255)
                                                                                                                    cTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCaa                       <  1:561118/141‑1 (MQ=255)
                                                                                                                     tGTAACATAAAACCATGTTTCCACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAAt                      <  1:270750/141‑1 (MQ=255)
                                                                                                                       tATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTc                    >  1:516973/1‑141 (MQ=255)
                                                                                                                       tATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGAACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTc                    >  2:193496/1‑141 (MQ=255)
                                                                                                                         tCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTa                  >  2:182277/1‑141 (MQ=255)
                                                                                                                          cATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTaa                 >  1:413676/1‑141 (MQ=255)
                                                                                                                              aacccttttttCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTg             <  2:25812/135‑1 (MQ=255)
                                                                                                                                aCCATTTTTCTACTTCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCTTCTAACGCAACATATGTTAAGTGGCTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTGAATTCTTATTTgga           >  2:50261/1‑139 (MQ=255)
                                                                                                                                aCCATTTTTCAACATCATCTTCTGGATATACACCAGGTACCTCTTTTGATTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGaa           >  1:628240/1‑141 (MQ=255)
                                                                                                                                aCCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGaa           >  1:3673/1‑141 (MQ=255)
                                                                                                                                    tttttCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCTGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATCTGAACGAc       <  2:492280/141‑1 (MQ=255)
                                                                                                                                        tCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACCACt   >  1:478341/1‑141 (MQ=255)
                                                                                                                                        tCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACCACt   <  1:327856/141‑1 (MQ=255)
                                                                                                                                         cAACACCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACAACtt  >  2:79262/1‑141 (MQ=255)
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CCTTAACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACCACTT  >  CP000730/2021677‑2021954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑