Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,373,081 A→G 7.0% I232T (ATT→ACT)  USA300HOU_2234 ← NCS2 family nucleobase:cation symporter‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,373,0810AG 7.0% 154.4 / 3.2 57I232T (ATT→ACT) USA300HOU_2234NCS2 family nucleobase:cation symporter‑2
Reads supporting (aligned to +/- strand):  ref base A (29/24);  new base G (2/2);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01

GTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGTG‑ATGATCATACCGATAAAGATAGAACCCGGCAATTTAATTGCATATAAAATAACTGTAA  >  CP000730/2372943‑2373210
                                                                                                                                          |                                                                                                                                  
gTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATa                                                                                                                                  <  1:647563/141‑1 (MQ=255)
gTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTAAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATa                                                                                                                                  >  2:573985/1‑141 (MQ=255)
  aGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACt                                                                                                                                >  1:340059/1‑141 (MQ=255)
   gCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACtt                                                                                                                               <  1:527817/141‑1 (MQ=255)
     aaCAGCTACTAATGTACCAGCTGTATCAAAGGAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTgg                                                                                                                             <  1:50513/141‑1 (MQ=255)
         gCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATc                                                                                                                         <  1:106207/141‑1 (MQ=255)
         gCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATc                                                                                                                         >  2:95244/1‑141 (MQ=255)
         gCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGATAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATc                                                                                                                         >  2:238219/1‑141 (MQ=255)
            actaATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTa                                                                                                                      >  1:588589/1‑141 (MQ=255)
                 tGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACAGAACGTTGGTTCAATGCTTGGAATCTTGCCcac                                                                                                                 >  1:397052/1‑141 (MQ=255)
                  gTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCcaca                                                                                                                >  2:220578/1‑141 (MQ=255)
                       aGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATAcc                                                                                                           >  1:191055/1‑141 (MQ=255)
                            taacAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACTTTGGTTTTATGCGTGGAATCTTACCCACAATACCAGATg                                                                                                      <  1:324056/138‑1 (MQ=255)
                            tATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATg                                                                                                      <  2:347462/141‑1 (MQ=255)
                              acAAAGAAATCAATAAAAAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGc                                                                                                    <  1:165046/140‑1 (MQ=255)
                                  aGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCAttt                                                                                                >  1:336218/1‑141 (MQ=255)
                                   gAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGCCATTTg                                                                                               >  2:122991/1‑141 (MQ=255)
                                    aaaTCCATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGa                                                                                              <  1:531225/141‑1 (MQ=255)
                                              ataaGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACACACAATACCAGCTGGCATTTGAATGAATcctg                                                                                    >  2:94890/1‑139 (MQ=255)
                                                  gAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGaa                                                                                >  1:457558/1‑141 (MQ=255)
                                                       ttAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATAc                                                                           <  1:617230/141‑1 (MQ=255)
                                                          aaaTAGCAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCaa                                                                        <  2:486577/141‑1 (MQ=255)
                                                          aaaTAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCAAGTGAACATACCaa                                                                        >  2:70980/1‑141 (MQ=255)
                                                           aaTAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAAc                                                                       >  2:352593/1‑141 (MQ=255)
                                                            aTAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTAAACATACCAACg                                                                      >  2:362802/1‑141 (MQ=255)
                                                               aCAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCcaca                                                                                                                >  1:560051/1‑96 (MQ=255)
                                                               aCAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCcaca                                                                                                                <  2:560051/96‑1 (MQ=255)
                                                               aCAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCGCAATACCAGATGGCCTTTGAATCAATCCGGTGAAAATACAAGCGAtt                                                                   >  2:588564/1‑141 (MQ=255)
                                                               aCAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGAtt                                                                   <  1:556155/141‑1 (MQ=255)
                                                               aCAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGGTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGAtt                                                                   <  2:499860/141‑1 (MQ=255)
                                                                cAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTg                                                                  <  2:336218/141‑1 (MQ=255)
                                                                 aaTTAAGAACTGTATCGTTAATAATTGACTTGTGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGc                                                                 <  1:54695/141‑1 (MQ=255)
                                                                  aTTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAGTACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCt                                                                >  2:169927/1‑141 (MQ=255)
                                                                  aTTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAGTACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCt                                                                >  2:544498/1‑141 (MQ=255)
                                                                  aTTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCt                                                                <  1:220578/141‑1 (MQ=255)
                                                                  aTTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCt                                                                <  1:571127/141‑1 (MQ=255)
                                                                       gAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGtg‑at                                                          >  2:18192/1‑141 (MQ=255)
                                                                        accTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAAGCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGtg‑atg                                                         <  2:463242/139‑1 (MQ=255)
                                                                          cTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGTg‑atgat                                                       <  1:85132/141‑1 (MQ=255)
                                                                              aTCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTAGACACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTACTTTG‑ATGATCATa                                                   >  2:76673/1‑141 (MQ=255)
                                                                              aTCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGTG‑ATGATCATa                                                   >  2:322562/1‑141 (MQ=255)
                                                                               tCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACGAAAGATTGCTGTG‑ATGATCATAc                                                  >  2:433690/1‑141 (MQ=255)
                                                                                 gTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGGTGCTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCCGTGAACATACCAACGATTGCTGTG‑ATGATCATACCg                                                >  1:520114/1‑141 (MQ=255)
                                                                                                 ggTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGAGGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGTG‑ATGATCATACCGATAAAGATAGAACCCg                                >  1:256774/1‑141 (MQ=255)
                                                                                                   tCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGTG‑ATGATCATACCGATAAAGATAGAACCCGGc                              >  2:590175/1‑141 (MQ=255)
                                                                                                      ttAAACGCTTCAAATGCAGCCCCGAACGGTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGTG‑ATGATCATACCGATAAAGATAGAACCCGGCAAt                           <  2:303892/141‑1 (MQ=255)
                                                                                                         aaCGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGTG‑ATGATCATACCGATAAAGATAGAACCCGGCAATTTa                        >  1:63674/1‑141 (MQ=255)
                                                                                                          aCGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGTG‑ATGATCATACCGATAAAGATAGAACCCGGCAATTTaa                       <  2:295470/141‑1 (MQ=255)
                                                                                                           cGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGTG‑ATGATCATACCGATAAAGATAGAACCCGGCAATTTAAt                      <  1:576983/141‑1 (MQ=255)
                                                                                                                 aaaTGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACTCACGATTGCTGTG‑ATGATCATACCGATAAAGATAGACCCCGGCAATTTACTTGCatt                >  1:412025/1‑140 (MQ=255)
                                                                                                                  aaTGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACAAACGATTGCTGTG‑ATGGTCATACCGATAAAGATAGAACCCGGCAATTTAATTGCatat               >  1:139811/1‑141 (MQ=255)
                                                                                                                  aaTGCAGCACCGAACGTTGGTTCAATACTTGGAACCTTACCAACACTACCAGATGGCATTTGAACAAGACCCTTGAACAGA‑CACCTGTCGGTCTGCATTATCATACCGATAAAGATAGAACCCGGCAATTTAATTGCatat               <  1:321273/141‑1 (MQ=255)
                                                                                                                      cagcaCCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGTG‑ATGATCATACCGATAAAGATAGAACCCGGCAATTTAATTGCATATaaaa           <  2:664876/141‑1 (MQ=255)
                                                                                                                           ccGAACGTTGGTTCAGTACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGTG‑ATGATCATACCGATAAAGATAGACCCCGGCAATTTAATTGCATATAAAATAACt      <  1:544498/141‑1 (MQ=255)
                                                                                                                           ccGAACGTTGGTTCAGTACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGTG‑ATGATCATACCGATAAAGATAGAACCCGGCAATTTAATTGCATATAAAATAACt      <  1:169927/141‑1 (MQ=255)
                                                                                                                             gAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGTG‑ATGAGCATTCCGATAACGATAGAACCCGGCAATTTAATTGCATGTAAAATAACTGt    >  1:152015/1‑141 (MQ=255)
                                                                                                                               aCGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGTG‑ATGATCATACCGATAAAGATAGAACCCGGCAATTTAATTGCATATAAAATAACTGtaa  >  2:509211/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                  
GTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGTG‑ATGATCATACCGATAAAGATAGAACCCGGCAATTTAATTGCATATAAAATAACTGTAA  >  CP000730/2372943‑2373210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑