Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,532,2980AT100.0% 250.9 / ‑6.5 83T15T (ACA→ACTUSA300HOU_2392FemAB family peptidoglycan biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (26/55);  minor base G (1/0);  total (28/55)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACG  >  CP000730/2532163‑2532435
                                                                                                                                       |                                                                                                                                         
tgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTTACGTTGACTTCGGATGAGCTCTATGCGTTTACTACaaa                                                                                                                                      <  1:372874/141‑1 (MQ=255)
 ttATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                                     <  2:380107/140‑1 (MQ=255)
 gtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                                     <  1:362304/141‑1 (MQ=255)
 gtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                                     <  1:29241/141‑1 (MQ=255)
 ggaTTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATTCGTTTACTACAAAg                                                                                                                                     <  2:165903/139‑1 (MQ=255)
     ttGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAtt                                                                                                                                 >  2:633588/1‑141 (MQ=255)
        tAATCAGTGTTTGATAAAATAAAAAAATAGCAGTATTAATAAATTCAAAAATTATAGTTCAACTCAGAGAGGGATGGGATAAAAGGAAACCTGCAACGTTGACTTCGGATGAGTTCAATGCGTCTACTAAAAACCAttttt                                                                                                                              >  2:244053/1‑141 (MQ=255)
         aaacAGTTTTTGATAAAATAAAAAAATAGCATTATTACTAACTTACTACATTATCTTTGGATTCAGAGAGGGATGGGATACAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc                                                                                                                             <  2:640267/138‑1 (MQ=255)
           tCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTcac                                                                                                                           <  1:311591/141‑1 (MQ=255)
              gatttcaatagaataaaAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATACAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGAGTTCGGATGAGTTCAATGAGTTTACTACAAAGCATTTTTCACAtt                                                                                                                        >  2:245047/8‑141 (MQ=255)
              gTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACAtt                                                                                                                        <  2:626475/141‑1 (MQ=255)
               tttttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTa                                                                                                                       <  2:267472/141‑1 (MQ=255)
               tttttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTa                                                                                                                       <  1:514/141‑1 (MQ=255)
                 tttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTaca                                                                                                                     <  1:546306/141‑1 (MQ=255)
                  ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGGTGAGTTCAATGCGTTTGCTACAAAGCATTTTTCACATTacaa                                                                                                                    >  1:346930/1‑140 (MQ=255)
                  ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac                                                                                                                    <  1:556102/141‑1 (MQ=255)
                   tGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                                   >  2:99190/1‑141 (MQ=255)
                   tGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                                   <  1:361079/141‑1 (MQ=255)
                   tGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                                   <  1:110430/141‑1 (MQ=255)
                       aaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAAt                                                                                                               <  1:189100/141‑1 (MQ=255)
                        aaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATc                                                                                                              <  2:214979/141‑1 (MQ=255)
                         aataaaaAAATAGCATTATTAATAAATTGAGAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCa                                                                                                             <  2:271367/141‑1 (MQ=255)
                         aataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCa                                                                                                             <  1:57160/141‑1 (MQ=255)
                         aataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCa                                                                                                             <  1:65432/141‑1 (MQ=255)
                         aaaaaaaaaaTAGCATGATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCa                                                                                                             <  2:271290/138‑1 (MQ=255)
                           taaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAACTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGc                                                                                                           >  2:104567/1‑141 (MQ=255)
                           taaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGc                                                                                                           <  2:496516/141‑1 (MQ=255)
                           aaaaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGc                                                                                                           <  2:357271/140‑1 (MQ=255)
                            aaaaaaaTAGCATCATGAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCt                                                                                                          <  2:316953/141‑1 (MQ=255)
                             aaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGCGTTAAATGCGTGTACGACAAAGCATTTTTCACATTACACACAATCAGCTa                                                                                                         >  1:123216/1‑141 (MQ=255)
                             aaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTa                                                                                                         >  1:663022/1‑141 (MQ=255)
                              aaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAt                                                                                                        >  1:232503/1‑141 (MQ=255)
                              aaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAt                                                                                                        <  2:90339/141‑1 (MQ=255)
                                 aagaGCATTATTAATAAATTCAAAAATTATAGTTCAATCCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGGTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCa                                                                                                     <  2:459452/138‑1 (MQ=255)
                                        ttattaATAAATTCAAAAATTATAGTGCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAt                                                                                              <  2:224130/141‑1 (MQ=255)
                                         tattaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATc                                                                                             <  1:646789/141‑1 (MQ=255)
                                           ttaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCAt                                                                                           <  2:279160/141‑1 (MQ=255)
                                              ataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTTTTTTTTTTTTCAATCAGATATTCATTACAATCATaga                                                                                        >  2:479567/1‑141 (MQ=255)
                                                aaaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACATCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTCTTCATTACAATCATAGAGt                                                                                      >  2:105436/1‑141 (MQ=255)
                                                       aaaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTCCTACAAAGCATTTTTAATATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTa                                                                               <  2:260968/141‑1 (MQ=255)
                                                        aaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaa                                                                              >  1:522833/1‑141 (MQ=255)
                                                             ataGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGc                                                                         >  1:484486/1‑141 (MQ=255)
                                                              taGTTCAATTCAGGGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg                                                                        <  1:220361/141‑1 (MQ=255)
                                                                gTTCAATTCAGCGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAt                                                                      <  1:223409/141‑1 (MQ=255)
                                                                gTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAt                                                                      <  1:125182/141‑1 (MQ=255)
                                                                 ttcaattcaaAGAGGGATGGGATAAAATGAAATTTGTAACGTTGACTCCCGATGAGTTCCATGCCTTTACTACAACGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtg                                                                     <  2:679/141‑1 (MQ=255)
                                                                  tCAAGTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACAGGACACACAATCAGCTATTCATTACAATCATCGAGTGGAGTTAAAAGGCGAtgt                                                                    <  1:461473/141‑1 (MQ=255)
                                                                    taTTCCGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGc                                                                  <  2:129026/140‑1 (MQ=255)
                                                                       tCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATc                                                               <  1:636178/141‑1 (MQ=255)
                                                                         agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGGGTTTAATACAAAGAATTGTTCACGTTACACACAATCAGGTATTCATTAAGATCCTCGAGTCGGGTTAAAAGGCGATGTGCCTCtt                                                             >  2:72436/1‑141 (MQ=255)
                                                                          gagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGCTGATTTCAAAGGCGATGTGCATCTTg                                                            >  2:264961/1‑141 (MQ=255)
                                                                          gagagGGATGGGATAAAATGAACTTTGTAACGGTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTg                                                            <  1:425487/141‑1 (MQ=255)
                                                                               ggatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAggg                                                       <  1:312173/141‑1 (MQ=255)
                                                                                gatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                     <  2:381989/141‑1 (MQ=255)
                                                                                 atgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                     <  1:143584/141‑1 (MQ=255)
                                                                                  tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGtt                                                    <  2:232503/141‑1 (MQ=255)
                                                                                  tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGtt                                                    >  1:331758/1‑141 (MQ=255)
                                                                                   gggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTa                                                                                                         >  2:352329/1‑87 (MQ=255)
                                                                                   gggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTa                                                                                                         <  1:352329/87‑1 (MQ=255)
                                                                                   gggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGACGTGCATCTTGTAGGGGTTa                                                   >  2:632861/1‑141 (MQ=255)
                                                                                   gggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGACGATCTGCATCCTGTAGGGGTTa                                                   >  2:253110/1‑141 (MQ=255)
                                                                                      atAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTCCTACAAAGCATTTTTCACATTCCACACAATCATCGATTCATTACAATCAGAGAGGGGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAg                                                >  1:356251/1‑141 (MQ=255)
                                                                                        aaaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTTCAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTCAAAgtt                                              >  2:265747/1‑139 (MQ=255)
                                                                                         aaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                             <  2:400049/141‑1 (MQ=255)
                                                                                          aaTGAACTTTTTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTGTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGa                                            <  2:84226/141‑1 (MQ=255)
                                                                                            tGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACa                                          <  2:121902/141‑1 (MQ=255)
                                                                                               aCTTTGTAACGTTGACTTCGGATGAGTTCAATGCCTTTACCACAAAGCAGTTTTCACATTACACACAAACAGCTATTCATTACAATCATAGAGCTGATTTAAAAGGCGATGTGCATCTTGTATGGGTTAAAGATGACAATg                                       >  2:654769/1‑141 (MQ=255)
                                                                                                     tAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACAAACACTCAGCTATTCATTACAATCTTAGAGTTGATTGAAAAGTCGATGTGCATCTTGGAGGGGTTAAAGATGACAAAGGTCAAg                                 >  2:321783/1‑141 (MQ=255)
                                                                                                         gTTGACTTGGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAt                             <  2:346930/141‑1 (MQ=255)
                                                                                                          ttagcTTCGGATAAGTTCAATCCGTTTACTACAAAGCATTTTTCATATTACACACAATCAGCTATTCATTACAATCCTAGGGTTGTTTTCAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAtt                            <  2:367204/137‑1 (MQ=255)
                                                                                                          tggACTTCGGATGAGTTCTATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAtt                            <  2:367240/139‑1 (MQ=255)
                                                                                                            gACTTCGGATGAACTCAATGCGCTTACTAAAAAGCATATTTCACATTACCCACCCTCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGc                          <  1:468615/141‑1 (MQ=255)
                                                                                                              cTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGGTGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATGGCAg                        >  1:2338/1‑141 (MQ=255)
                                                                                                                tCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGa                      <  2:203581/141‑1 (MQ=255)
                                                                                                                tCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACAAGTACTGCTAGTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGa                      <  1:19117/141‑1 (MQ=255)
                                                                                                                 cGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt                     >  1:669384/1‑141 (MQ=255)
                                                                                                                  ggATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCCTCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATg                    <  2:558185/141‑1 (MQ=255)
                                                                                                                  ggATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATCGCAGGATg                    >  2:104710/1‑141 (MQ=255)
                                                                                                                  cgATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATg                    <  2:28452/140‑1 (MQ=255)
                                                                                                                   gATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCCGGATGc                   >  1:579965/1‑141 (MQ=255)
                                                                                                                   gATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGc                   >  2:46730/1‑141 (MQ=255)
                                                                                                                        gtgcAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTTATTACAATCATAGAGTTGATTTAAATGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAtt              <  1:479567/138‑1 (MQ=255)
                                                                                                                              tGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGa        <  2:170777/141‑1 (MQ=255)
                                                                                                                                    tactacAAAGCATTTTTCACATTACACACAATCAGCTATTCATAACAATCATAGAGTTGATTTAAAACGCGATGTGCATCTTGTAGGAGGAAAAGATGACAGTGGTCAAGTGATTACAGGATGCTTATTTACAGAAgcacg  >  2:23603/1‑141 (MQ=255)
                                                                                                                                    tactacAAAGCATTTTTCACAGTACACACAATCAGCTATTCATTACAATCATATAGTTGAATTAAAAGGCGATGTGCATCTTGAAGGGGGTAAAGATGCCAATGGACAAGTGAATGCCGGATGCTTATTTACAGAAggccg  >  2:225874/1‑137 (MQ=255)
                                                                                                                                       |                                                                                                                                         
TGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACG  >  CP000730/2532163‑2532435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: