Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,679,267 C→T 12.5% intergenic (+139/‑250) USA300HOU_2535 → / → USA300HOU_2536 hypothetical protein/possible secretory antigen

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,679,2670CT12.5% 182.5 / 4.8 71intergenic (+139/‑250)USA300HOU_2535/USA300HOU_2536hypothetical protein/possible secretory antigen
Reads supporting (aligned to +/- strand):  ref base C (28/33);  new base T (8/2);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 8.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACATGAATACATGGAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATAT  >  CP000730/2679129‑2679404
                                                                                                                                           |                                                                                                                                         
tACATGAATACATGGAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCAt                                                                                                                                         >  2:251999/1‑141 (MQ=255)
   aTGAATACATGGAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTAATTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCAtttt                                                                                                                                      <  2:169789/141‑1 (MQ=255)
       gtACATTGGGTCTAATCATTTCATTTTAGTGCTCTGATTGCTGGGCGCTAGTTTTAATGTTATTGCTACATTTAGTATATGGTGATTTCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTatat                                                                                                                                  <  2:455205/140‑1 (MQ=255)
       aTACATGGAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTatat                                                                                                                                  <  2:472607/141‑1 (MQ=255)
         aCATGGAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATAttt                                                                                                                                >  1:533937/1‑141 (MQ=255)
           aTGGAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTc                                                                                                                              >  1:106179/1‑141 (MQ=255)
            tGGAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCCTTTTTTATTTTcc                                                                                                                             >  1:218035/1‑141 (MQ=255)
             ggAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGATCCATGTACCTTTTTTTTAAATTATTTTTTATTTTccc                                                                                                                            >  2:139148/1‑141 (MQ=255)
             ggAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTccc                                                                                                                            >  1:116673/1‑141 (MQ=255)
             ggAGTCACATCATCTCATGCAAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTccc                                                                                                                            >  1:434210/1‑141 (MQ=255)
                gTCACCTCATCTCATGTGAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTATAATACTATTTTATATTTTCCCACt                                                                                                                         <  2:644813/141‑1 (MQ=255)
                gTCACAGCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACt                                                                                                                         <  1:570192/141‑1 (MQ=255)
                 tCACATTAGCGCATGCTAGCGCTCTGATTCCAGGGCGCTATTTTGAAGGTGATTGCGGCATTTAGTATATTGTGATTGCTAGTTTCTTGCTGTGCGAGATCCTTGTACCTTTTTTTTTCCTTATTTTATATTTccctatta                                                                                                                        <  2:51290/141‑6 (MQ=255)
                   acaTCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAATTCATTTTTTATTTTCCCACTAat                                                                                                                      >  2:521311/1‑141 (MQ=255)
                    catcatCTCATGTTAGCACTCTGATTGCACGGCGTTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAACTCATTTTATATTTTCCCACTAata                                                                                                                     <  2:112006/141‑1 (MQ=255)
                       catcTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATCTTTTCTTCCTATTATcc                                                                                                                  >  2:563837/1‑141 (MQ=255)
                           tcATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTg                                                                                                              <  1:210929/141‑1 (MQ=255)
                            cATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTAACTTTTTTTTAAATCATTTTATATTTTCATCCTAATATCCGCtat                                                                                                             >  1:342137/1‑139 (MQ=255)
                              tGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTa                                                                                                           <  1:311190/141‑1 (MQ=255)
                              tGTTAGCGCTCAGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTa                                                                                                           <  1:6368/141‑1 (MQ=255)
                                    cgcTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTaatcaat                                                                                                     >  1:181769/1‑141 (MQ=255)
                                     gcTTTGGTTGGAGGGCGCTATTTTTTATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTaatcaatc                                                                                                    <  1:219260/141‑1 (MQ=255)
                                     cctctGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTaatcaatc                                                                                                    <  1:375664/140‑1 (MQ=255)
                                       cctGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCt                                                                                                  <  1:156090/140‑1 (MQ=255)
                                          gATTGCAGGTCTCTATTTTTAATGTTATTGCTACATTTAGTATATTGTTATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGAc                                                                                               <  1:37078/141‑1 (MQ=255)
                                           aTTGCAGGGCGCTATTATATATATAAATGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACa                                                                                              <  1:362270/141‑1 (MQ=255)
                                             tGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATc                                                                                            <  2:434210/141‑1 (MQ=255)
                                             tGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCACCCCGACTTCCTCTGTTAATCAATCCTGACATc                                                                                            <  1:324019/141‑1 (MQ=255)
                                                  ggCGCTATTTTTAAGGTTATTGCTACATTGAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGt                                                                                       >  1:358272/1‑141 (MQ=255)
                                                    cgcTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTAt                                                                                     <  2:461139/141‑1 (MQ=255)
                                                       tATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCACTCCTGAAATCCTTGTATCat                                                                                  >  2:381206/1‑140 (MQ=255)
                                                        aTTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACt                                                                                 >  1:218817/1‑141 (MQ=255)
                                                          ttttAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTAt                                                                               <  1:602542/141‑1 (MQ=255)
                                                          ttttAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGAAATCCTTGTATCACTAt                                                                               >  1:511388/1‑141 (MQ=255)
                                                              aaTGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACa                                                                           >  1:35304/1‑141 (MQ=255)
                                                                 gTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTCACATCCTTGTATCACTATGACaatt                                                                        >  1:62274/1‑141 (MQ=255)
                                                                  tcaTTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACaatta                                                                       <  1:149001/139‑1 (MQ=255)
                                                                          aCATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTTATTTATTTTATATTTTCCCACTAATATCCGCTTTTTATCAATCCTTAGATCCTTGTATCACTATGACCATTAATTGTTaa                                                               >  2:375460/1‑141 (MQ=255)
                                                                           cATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTaaa                                                              <  2:230321/141‑1 (MQ=255)
                                                                             tttAGTATATTGTGATTGCTATTTTCTGTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACGAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATa                                                            <  1:590419/141‑1 (MQ=255)
                                                                                 gTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATg                                                        >  1:498022/1‑141 (MQ=255)
                                                                                     atTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAAtt                                                    >  1:365710/1‑141 (MQ=255)
                                                                                         tgATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTTTATTTTTTTTTATTTACTCACTAATATCCTTTTTTCATTATTTATGGTGTTCTTGTATCAATATGACAATTTATTATCAAATACATGTAtttttt                                                >  2:153183/1‑138 (MQ=255)
                                                                                          gATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTTTTTTTTTTTATATTTTCCCACTAATATCCGCTGTTAATCGATCCTGACGTCCTTGTATTCCTCTGCCCATTAATTGTTAAATACATGAGTTTTTAc                                               >  2:193751/1‑141 (MQ=255)
                                                                                             tGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACAt                                             >  1:577413/1‑141 (MQ=255)
                                                                                              gCTATTTTCTTTCAGTTATAGAACCCTGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACTATTAATTGTTAAATACATGAATTTCTACAttt                                           >  2:287109/1‑141 (MQ=255)
                                                                                                 aTTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTTTTTTTTTTGATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGGCATCCTTGTATCACTATTTCAATTAATTGTTAAATACATTTATTTTTACTTTTTAt                                        >  2:426893/1‑141 (MQ=255)
                                                                                                 aTTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTTTATTTTTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTCTCACTATGTCCATTAATTGTTAAATACATTTATTTCTACGTTTTAt                                        >  2:8347/1‑141 (MQ=255)
                                                                                                 aTTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATGGTTAAATACATGAACTTCTACATTTTAt                                        >  2:326895/1‑141 (MQ=255)
                                                                                                  ttttttttCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATg                                       <  2:512887/141‑1 (MQ=255)
                                                                                                  tttgCTTTTCGTTCTACATCCCTGTTCCTTTTCTTTAAATCATTTTATCTTTTCCCCCTAATATCCGCTGTTAATCAATCCTGACATCCTTTTATCACTATGTCAAGTTATTGTTAAATACATGATTTTCTACATTTTATg                                       <  2:652565/141‑1 (MQ=255)
                                                                                                       tttcAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGaaaaa                                  >  1:647470/1‑141 (MQ=255)
                                                                                                        ttcACTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGTCAATTAATTGTTAAATACATGAATTTCTAAAATTaaaaaaaaaa                                 <  2:358272/141‑1 (MQ=255)
                                                                                                         tcAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAAt                                >  1:68742/1‑141 (MQ=255)
                                                                                                         tcAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTAACCATAAAACCATTGATATAGAAAAACATGAATTTCTACATTTTATGAAAAAAt                                >  2:303033/1‑141 (MQ=255)
                                                                                                         tcAGTTATAGAACCATGTACCTTTTTTTTAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATc                               <  1:176140/141‑1 (MQ=255)
                                                                                                                 aGAACCATGTACCTTTTTTTTTTATTTTTTTATATTTTCCCACTAATATCCGCTTTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTATATTTTATGAAAAATTCTAttttt                        >  2:553508/1‑141 (MQ=255)
                                                                                                                    aCCATGTACCTTTTTTTTAAATCATTTTATTTTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTCATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTttctt                     <  2:491895/141‑1 (MQ=255)
                                                                                                                      cttGTTCCTTTTTTTTAAATCATTTTACTTCTACCCACTAATATCCGCTCTTAATCAATCCTGACATTCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTAc                   <  1:172869/139‑1 (MQ=255)
                                                                                                                      cATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTAc                   <  1:202069/141‑1 (MQ=255)
                                                                                                                        tGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCAATTTTTATTACaa                 >  1:585876/1‑141 (MQ=255)
                                                                                                                          tACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAAtt               >  1:263929/1‑141 (MQ=255)
                                                                                                                          tACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAAtt               <  1:206280/141‑1 (MQ=255)
                                                                                                                           aCCTTTTTTTTTTTTTTTTTTATATTTTGCCGCCAATATCCGCTGTTAATGAATCCTGACGTCCTTGTATCACTATTACAATTAATTGTTAAATACATGCATTTTTTCATTTTTTGATAAATTCCATTTTTATTACAAttt              >  2:196297/1‑140 (MQ=255)
                                                                                                                           aCCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATGCATTTTTATTACAAtta              >  1:260603/1‑140 (MQ=255)
                                                                                                                           aCCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTc              <  1:251999/141‑1 (MQ=255)
                                                                                                                           aCCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTc              <  1:574069/141‑1 (MQ=255)
                                                                                                                             cTTTTTTTTAAATCATTTTGTATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAGAAATCCATTTTTATTACAATTCaa            <  1:426893/141‑1 (MQ=255)
                                                                                                                                    tttAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACttt      >  1:144637/1‑141 (MQ=255)
                                                                                                                                     ttAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTATACATTGTATGAAAAAATCCATTTTTATTACAATTCAACACTTta     <  1:459333/141‑1 (MQ=255)
                                                                                                                                        aaTCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTatat  <  2:196570/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TACATGAATACATGGAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATAT  >  CP000730/2679129‑2679404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: