| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 757,864 | T→C | 9.1% | T380T (ACT→ACC) | USA300HOU_0707 → | ABC superfamily ATP binding cassette transporter, ABC protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 757,864 | 0 | T | C | 9.1% | 141.3 / 6.8 | 57 | T380T (ACT→ACC) | USA300HOU_0707 | ABC superfamily ATP binding cassette transporter, ABC protein |
| Reads supporting (aligned to +/- strand): ref base T (33/18); new base C (3/2); total (37/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.84e-01 | |||||||||||
GATAGATTGGTATTAAATGATTTAAATTTGGAAATATTTAAAGGTGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATAGAAAATAATACTATTGAAGAAGTGCTAGACGAAGTAGG > CP000730/757730‑758004 | gATAGATTGGTATTAAATGATTTAAATTTGGAAATATTTAAAGGTGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATTCCCGCTTGTACATCTTATTGCAGGAGTTGATCAGCCAACAATAGGTACTATAAGt > 1:549937/1‑141 (MQ=255) tGGTATTAAATGATTTAAATTTGGAAATATTTAAAGGTGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAAcc < 2:294412/141‑1 (MQ=255) ggTATTAAATGATTTAACTTTGGAAATAGTTAAAGGTGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATGAGCCAGCAATAGGTACTATAAATAGAAAACa > 1:147625/1‑141 (MQ=255) aTTAAATGATTTAAATTTGGAAATATTTAAAGGTGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCg < 2:138054/141‑1 (MQ=255) aaTGATTTAAATTTGGAAATATTTAAAGGTGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGAt < 2:430114/141‑1 (MQ=255) aaTGATTTAAATTTGGAAATATTTAAAGGTGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGAt < 1:92400/141‑1 (MQ=255) aTGATTTAAATTTGGAAATATTTAAAGGTGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGAtt > 1:205409/1‑141 (MQ=255) aTTTAAATTTGGAAATATTTAAAGGTGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTGAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTaa > 2:16074/1‑141 (MQ=255) aTTTAAATTTGGAAATATTTAAAGGTGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTaa > 1:439666/1‑141 (MQ=255) aTTTAAATTTGGAAATATTTAAAGGTGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGGGATTTaa > 2:234672/1‑141 (MQ=255) aaaTTTGGAAATATTTAAAGGTGATCAACTTGCACTTGTTGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCCAGCGTTTATCAGACAACAATAGGTACTATAAGTACAAACCCGCGTGATTTAAatat < 2:2328/141‑1 (MQ=255) aaTTTGGAAATATTTAAAGGTGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAAtata > 1:55306/1‑141 (MQ=255) tttGGAAATATTTAAAGGTGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAgg < 2:429716/141‑1 (MQ=255) tGGAAATATGTGAAGGTGATCAAAGTGCTCTTGTAGGTCCAAGCGGGGCCGGTAACTCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCGACAATAGGGGCTATAAGTACAAACCAACGTGATTTAAGTATAGGaa > 1:358278/1‑141 (MQ=255) ggAAATATTTAAAGGTGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAGCCCAGCGTGATTTAAATATAAGAAt > 2:419660/1‑141 (MQ=255) ggAAATATTTAAAGGTGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACCATAAGTACAAACCAGCGTGATTTAAATATAGGAAt > 2:318685/1‑141 (MQ=255) ggAAATATTTAAAGGTGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACCATAAGTACAAACCAGCGTGATTTAAATATAGGAAt > 2:318581/1‑141 (MQ=255) gAAATATTTAAAGGTGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGGGGTTATCAGCCAACAATAGGGACTATAAGTACAAACCAGCGAGATTTAAAGATAGGAATa > 1:197528/1‑141 (MQ=255) aaGGTGATCAAATTGCATTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGCAACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCATCGTGATTTAAATATAGGAATACTTAGTCAAc < 1:88350/141‑1 (MQ=255) tGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGcc < 1:329254/141‑1 (MQ=255) gATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTCAATCCACTTTGACACATCTTATTGCAGGTGTTTCTCAGCCAACAATAGGTACTATAAGTACAAACCGGCGCGGTTTAAATATAGGCATACTTAGTCAAAAGccc > 2:149625/1‑140 (MQ=255) gATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCa > 1:136844/1‑141 (MQ=255) gATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATAAGCCAACAATAGGTACTGTAAGTACAACCCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCa > 2:249830/1‑141 (MQ=255) aTCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTACATATAGGAATAATTAGTCAACAGCCaa > 1:154547/1‑140 (MQ=255) aTCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATAGTTAGTCAACAGCCat > 1:359338/1‑141 (MQ=255) aCTTGAAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAg < 2:404781/141‑1 (MQ=255) gTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAAGAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGAGCTTCta > 2:194465/1‑141 (MQ=255) ccAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCGACGGTAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCtata < 2:539255/141‑1 (MQ=255) ccAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCtata > 1:431040/1‑141 (MQ=255) aaGCGGGGCAGGTAAATCCACTTTGACACACCTTATTGCCGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATaaa < 1:186653/141‑1 (MQ=255) aGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATaaaa > 2:58940/1‑141 (MQ=255) ggggCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAagag > 1:46928/1‑141 (MQ=255) gggCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAgcga > 1:383886/1‑141 (MQ=255) cAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTAAAAAAGAGAata > 1:318911/1‑141 (MQ=255) ggTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACGCAAATGCGTGATTTAAATATATGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATAtt < 1:268498/141‑1 (MQ=255) aaTCGACTTTGAGACATCTTATTGCAGGTGTTTATCAGCCAACAATCGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCATCAGCCGTATATTTTCAGTGCTTCTATAGAAGAGAATATTACGa > 1:395715/1‑141 (MQ=255) cacaTCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGata > 1:306014/1‑141 (MQ=255) acaTCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTAAAAACGAGAATATTACGATGTTTAAAGatat > 2:45912/1‑141 (MQ=255) aTCTTATTGCAAGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATAg < 2:519965/141‑1 (MQ=255) cTTATTGCAGGTGTTTATCAGCCAACAATAGGTACCATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATAGaa < 1:318581/141‑1 (MQ=255) cTTATTGCAGGTGTTTATCAGCCAACAATAGGTACCATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATAGaa < 1:318685/141‑1 (MQ=255) ttATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATAGaaa > 2:209469/1‑141 (MQ=255) aTTGCAGGTGTTTATCAGCCAACAATAGGTACCATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGTTATAGaaaaa > 2:52841/1‑140 (MQ=255) tGCAGGTGTTTATCAGCCAACCATAGGTACTAAAAGTACAAACCAGCGAGATAAAAAGATAGGAATACTTAGTAAACAGCAAAATATTTTCAGTGCTTCCATAAAAGAGAAGATTACGACGTTTAAAGATCTACAAaataa > 2:194462/1‑141 (MQ=255) ggTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATAGAAAATAATACt < 1:399180/141‑1 (MQ=255) gtTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGAGATAGAAAATAATACGAtt > 2:243463/1‑141 (MQ=255) cAGCCAACAATAGCTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATAGAAAATAATACTATTgaagaa < 2:549937/141‑1 (MQ=255) aGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATAGAAAATAATACTATTGaagaag < 2:348216/141‑1 (MQ=255) caacaaTAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATAGAAAATAATACTATTGAAGAAGTGc > 1:10582/1‑141 (MQ=255) aacaaTAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATGGGAAATAATACTATTGAAGAAGTGca > 1:154117/1‑140 (MQ=255) aacaaTAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATAG‑AAATAATGCTATTGAAGAAGTGCTa > 1:154171/1‑141 (MQ=255) aaTAGGTACTATAAGTACACACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATAGAAAATAATACTATTGAAGAAGTGCTAGa > 1:335675/1‑141 (MQ=255) tAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATAGAAAATAATACTATTGAAGAAGTGCTAGACg < 1:398910/141‑1 (MQ=255) tAGGTACAATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATAGAAAATAATACTATTGAAGAAGTGCTAGACg > 1:350435/1‑141 (MQ=255) tACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATAGAAAATAATACTATTGAAGAAGTGCTAGACGAAGt > 1:457108/1‑141 (MQ=255) tACTATAAGGACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATAGAAAATAATACTATTGAAGAAGTGCTAGACGAAGt < 2:54769/141‑1 (MQ=255) cTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATCGAAAATAATACTATTGAAGAAGTGCTAGACGAAGTAg > 1:290597/1‑141 (MQ=255) tataAGTACAAACCAGCTTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATAGAAAATAATACTATTGAAGAAGTGCTAGACGAAGTAgg < 1:525167/141‑1 (MQ=255) tataAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATAGAAAATAATACTATTGAAGAAGTGCTAGACGAAGTAgg < 2:295763/141‑1 (MQ=255) tataAGTACAAACCAGCGTGATTTAAAAATAGGAATACTTAGGCAACAGCCATATATTTTCAGTGCTTCTCTAAACGAGAATCATCCAAAGTTTAAAGCAGTACAGAATAATACGGTTGAAGAACAGATAGCGGAAGTAgg > 2:246758/1‑141 (MQ=255) | GATAGATTGGTATTAAATGATTTAAATTTGGAAATATTTAAAGGTGATCAAATTGCACTTGTAGGTCCAAGCGGGGCAGGTAAATCCACTTTGACACATCTTATTGCAGGTGTTTATCAGCCAACAATAGGTACTATAAGTACAAACCAGCGTGATTTAAATATAGGAATACTTAGTCAACAGCCATATATTTTCAGTGCTTCTATAAAAGAGAATATTACGATGTTTAAAGATATAGAAAATAATACTATTGAAGAAGTGCTAGACGAAGTAGG > CP000730/757730‑758004 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |