Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,232,465 G→C 100% A124P (GCG→CCG)  USA300HOU_1157 → non‑specific serine/threonine protein kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,232,4650GC100.0% 199.4 / ‑6.5 66A124P (GCG→CCG) USA300HOU_1157non‑specific serine/threonine protein kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (30/34);  minor base A (1/1);  total (31/35)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCATG  >  CP000730/1232332‑1232600
                                                                                                                                     |                                                                                                                                       
agaTGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGCAACGCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGa                                                                                                                                  <  1:552976/141‑1 (MQ=255)
agaTGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGa                                                                                                                                  <  2:517051/141‑1 (MQ=255)
    gACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTCGCTATCATTAAACATACGGATGATATg                                                                                                                              >  2:564032/1‑141 (MQ=255)
       tGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGt                                                                                                                           >  1:49288/1‑141 (MQ=255)
          ttctactTAGTAATGGAACATATCGAAGGTCCGACTTCGTCAGAGTATATTGAAAGTAATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATAAAATATTGGATGGCATTAAACATCCGCATGATATGCGTAtt                                                                                                                        <  1:68150/139‑1 (MQ=255)
               ctTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACa                                                                                                                   <  2:408981/141‑1 (MQ=255)
                  aGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTGATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATag                                                                                                                >  1:44901/1‑141 (MQ=255)
                        ggAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGatat                                                                                                          <  1:94780/141‑1 (MQ=255)
                        ggAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGatat                                                                                                          <  1:471691/141‑1 (MQ=255)
                        ggAATATAGCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGACATTAAGTGTTGACACAGCGATTAATGTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGatat                                                                                                          <  2:199317/141‑1 (MQ=255)
                         gAATATATCGAAGGTACGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCTTGATATGCGTATTGTACTTAGAGATAtt                                                                                                         >  1:311338/1‑141 (MQ=255)
                          aaTATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGAAATTa                                                                                                        >  1:72290/1‑141 (MQ=255)
                            tatatCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATAATACATGGAGATATTAAg                                                                                                      >  2:363037/1‑141 (MQ=255)
                             atatCGAAGGTCCGACTTTGTCTGAGAATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATCTAAAGc                                                                                                     >  1:262438/1‑141 (MQ=255)
                                tCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCcac                                                                                                  >  1:244635/1‑141 (MQ=255)
                                tCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTAACTAGAGATATTAAGCcac                                                                                                  >  1:36140/1‑141 (MQ=255)
                                    aGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATATTACCAATCAAATATTGAATGGCATTAAACATCCGCATGATATCCGCATTGTACATAAAGACATAAAGCCACaaaa                                                                                              >  2:127209/1‑141 (MQ=255)
                                     ggTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAat                                                                                             <  1:292055/141‑1 (MQ=255)
                                        ccGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatata                                                                                          >  2:476342/1‑141 (MQ=255)
                                             tttGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAAt                                                                                     <  2:517554/141‑1 (MQ=255)
                                                  ttGAGTAAATTGAAAGTCATGGGTCATAAAGGGTTGACAAAGCGACTAATTGTACGAATTAAAAATTGGATGGCATTAAAAATCCGTATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACa                                                                                <  1:255299/140‑1 (MQ=255)
                                                      gTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCaa                                                                            >  2:269869/1‑141 (MQ=255)
                                                        ttatTGAAAGTCATGGGCCATTAAGTGTTGACATAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATa                                                                          <  2:313861/140‑1 (MQ=255)
                                                        atatTGAAAGTCATGGGCCATTAAGTGTTGACATAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATa                                                                          <  2:313503/141‑1 (MQ=255)
                                                           ttGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATaaaa                                                                       >  1:65522/1‑141 (MQ=255)
                                                           ttGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATaaaa                                                                       <  1:256799/141‑1 (MQ=255)
                                                               aaGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGCCAGCAATAAAACGtt                                                                   <  1:416986/141‑1 (MQ=255)
                                                                aGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTg                                                                  >  1:187835/1‑141 (MQ=255)
                                                                aGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTg                                                                  >  1:468239/1‑141 (MQ=255)
                                                                  tCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGaa                                                                >  1:8283/1‑141 (MQ=255)
                                                                   cATGGGCCATTAAGTGTTGACACAGCGATTAGTTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGAGTATTGTACATAGAGATATTAAGCCACAAAATATTTTAATTGACAGCAATAAAACGTTGaaa                                                               >  2:273906/1‑141 (MQ=255)
                                                                   cATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCACTAAAACGTTGaaa                                                               >  2:448231/1‑141 (MQ=255)
                                                                   cATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGaaa                                                               <  2:9965/141‑1 (MQ=255)
                                                                     agggCCATTAAGTGATGACAAAGAGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAat                                                             <  1:78537/140‑1 (MQ=255)
                                                                        gCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATAtt                                                          >  1:416584/1‑141 (MQ=255)
                                                                        gCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATAtt                                                          >  2:210065/1‑141 (MQ=255)
                                                                             tAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGAtt                                                     <  2:247559/141‑1 (MQ=255)
                                                                                 tgtTGACACAGCGACTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAAATGACAGCAATAAAACGTTGAAAATATTTGATTTTgg                                                 >  1:310438/1‑141 (MQ=255)
                                                                                 gtggaacacAGGGATTAATTTTACGAGTCAATTCTTGGATGGTACTAGCCATCCGCATGATCTGCGTATCGTACACAGAGATATTAAGCGACAAAATATATTAATTGACAGCAACACAACGTTGAAAATATTTGATTTTgg                                                 <  2:226746/136‑1 (MQ=255)
                                                                                   ttGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGaa                                               >  2:86772/1‑141 (MQ=255)
                                                                                     gACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAAtt                                             >  1:402907/1‑141 (MQ=255)
                                                                                     gACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAAtt                                             >  2:60197/1‑141 (MQ=255)
                                                                                        acaGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAACATATATTAATTGACAGCAATCAAACGTTGAAACTATTTGATTTTGGAATTGcc                                          >  1:9900/1‑140 (MQ=255)
                                                                                         caGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGACATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTa                                         <  1:30962/141‑1 (MQ=255)
                                                                                          aGCGATTAATTTTACGAATCAAATATTGGATAGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAATACGTTGAAAATATTTGATTTTGGAATTGCTaa                                        <  2:280061/141‑1 (MQ=255)
                                                                                           gCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTaaa                                       <  1:564032/141‑1 (MQ=255)
                                                                                                tAATTTTACGAATCAAATATTGGATGGCATTAAACAGCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCttt                                  <  1:239986/141‑1 (MQ=255)
                                                                                                    tttACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGt                              <  2:526733/141‑1 (MQ=255)
                                                                                                             cAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCt                     >  2:32206/1‑141 (MQ=255)
                                                                                                              aaaaATTGGATGCAATAAAACAACCGCATGATATGCGTATTGTACAAAGTCATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCtt                    <  1:435164/141‑1 (MQ=255)
                                                                                                              aaaTATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCtt                    <  2:378872/141‑1 (MQ=255)
                                                                                                              aaaTATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCtt                    <  2:444407/141‑1 (MQ=255)
                                                                                                               aacatTGGATGCAATAAAACAAACGCAAGATATTGCAATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTGGAGAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCttt                   <  1:237453/138‑1 (MQ=255)
                                                                                                               aaTATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCttt                   <  1:99831/141‑1 (MQ=255)
                                                                                                                 aatTGGATGGCACTAAACACCCGCATGATATGCTGATTGTACAAAGAGATATTAAGCCACAAAATACATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTaa                 <  2:311338/140‑1 (MQ=255)
                                                                                                                  atTGGATGGCATTAAACATCCGCATGGTATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAAc                >  2:348737/1‑141 (MQ=255)
                                                                                                                  atTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAAc                >  2:52184/1‑141 (MQ=255)
                                                                                                                    tGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTc              <  2:49288/141‑1 (MQ=255)
                                                                                                                        tGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGAc          >  2:387625/1‑141 (MQ=255)
                                                                                                                           cATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATGAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTaa       <  1:153370/141‑1 (MQ=255)
                                                                                                                             ttAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATc     <  1:60197/141‑1 (MQ=255)
                                                                                                                             ttAAACATCCGCATGATATGCGTATTGTACAGAAAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATc     >  2:371358/1‑141 (MQ=255)
                                                                                                                             atAAAAATCCGCATGATATGCGTATTGTACCTAGAGATATTACGCCACAACATATCTTACTTGACAGCAATAAAACGTTTAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCACACTAATc     <  1:161047/140‑1 (MQ=255)
                                                                                                                              gaaaCATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCa    <  2:489840/140‑1 (MQ=255)
                                                                                                                               aaaCATCCGCATGATATGCGTATTGTACATAGAGATATTAATCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCAt   >  1:385081/1‑141 (MQ=255)
                                                                                                                                aaCATCCGCATGATATGCGTAGTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCAtg  <  2:323198/141‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
AGATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCATG  >  CP000730/1232332‑1232600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: