Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,416,4580AT100.0% 124.9 / NA 39I216N (ATT→AAT) USA300HOU_1314oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (19/20);  total (19/20)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTG  >  CP000730/1416321‑1416590
                                                                                                                                           |                                                                                                                                    
ttttAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGt                                                                                                                                   <  2:488637/141‑1 (MQ=255)
    aaCTCAATCATAGTCATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTg                                                                                                                               >  1:292773/1‑141 (MQ=255)
        cAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACaa                                                                                                                           <  2:291759/141‑1 (MQ=255)
         aaTCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATTCTTATGCAAATGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAat                                                                                                                          <  1:128618/141‑1 (MQ=255)
              tAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGAATGCAAATGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTc                                                                                                                     <  1:403772/141‑1 (MQ=255)
                     cccTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACAt                                                                                                              >  2:25569/1‑141 (MQ=255)
                      ccTCTTTAATTGTGTTCTATATTTAAATAGACGTTACGTATACGGATGCAAAGGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAAGGCTTTTACCTTCTTTTAAAACATAATTGTACTGACACTCTTTCAATACATg                                                                                                             >  2:349060/1‑141 (MQ=255)
                                 gtgtTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAt                                                                                                  >  2:522930/1‑141 (MQ=255)
                                         tatGTAATTACACGTTCTGTATACGGATGCAAATGCTCATACTTGAAATGATT‑AATAGTA‑CCTCGTTCAATGATTTGACCTACTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGata                                                                                          <  2:375503/141‑1 (MQ=255)
                                             tAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGCGTTCAGTTAGGTCTGAtaataa                                                                                      >  2:389938/1‑141 (MQ=255)
                                                 tAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAataaataa                                                                                  >  1:315775/1‑141 (MQ=255)
                                                        tCAGTATACGGATGCAAATGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACCTGACTTAAGTTATGTGTGATAATAAATATTGTTTGa                                                                           >  2:280927/1‑141 (MQ=255)
                                                        tCAGTATACGGATGCAAATGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGa                                                                           >  2:148260/1‑141 (MQ=255)
                                                          aGTATACGGATGCAAATGTTAATCCAGGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGtttagcc                                                                         >  2:398829/1‑137 (MQ=255)
                                                           gTATTCGGATGCAAATGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCa                                                                        <  1:251491/141‑1 (MQ=255)
                                                           gTATACGGATGCAAATGCTCATACGTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCa                                                                        <  1:251591/141‑1 (MQ=255)
                                                               aCGGATGCAAATGCTCATACTTGAAATGATTAAACAGCAGCCTAG‑‑CAATGCTCATACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTGTGACCATGtt                                                                    >  1:555016/1‑141 (MQ=255)
                                                                     gCAAATGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATTTGTGATAATAAATAATGTTTGACCATGTTCTAATa                                                              >  1:523554/1‑141 (MQ=255)
                                                                      cAAATGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATAc                                                             <  1:509605/141‑1 (MQ=255)
                                                                      aaaaTGATCATACTTGAAATGATT‑CATATTA‑CCTCGTTCAATGATTTGACCTTCTTTGAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGAGAATAAATAATGTTTGACCATGTTCTAATAc                                                             <  1:206487/140‑1 (MQ=255)
                                                                          tGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAata                                                         >  2:190613/1‑141 (MQ=255)
                                                                          tGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAata                                                         <  1:374460/141‑1 (MQ=255)
                                                                          tGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACAGAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAata                                                         <  1:107594/141‑1 (MQ=255)
                                                                              cATACTTGAAATTATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATCTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCt                                                     <  2:142432/141‑1 (MQ=255)
                                                                              cATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCt                                                     <  1:234793/141‑1 (MQ=255)
                                                                                  cTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGtaa                                                 <  1:447475/141‑1 (MQ=255)
                                                                                          gATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATATTCTAATACAATATGCTGTAATAAATCCa                                         >  1:405002/1‑141 (MQ=255)
                                                                                            tt‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATc                                       >  1:322984/1‑141 (MQ=255)
                                                                                                        cTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTAc                             >  2:424975/1‑141 (MQ=255)
                                                                                                        cTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTAc                             >  1:398247/1‑141 (MQ=255)
                                                                                                         ccGTTCAAAGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAGATTTCAACACATGTCTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTAcc                            <  2:60115/140‑1 (MQ=255)
                                                                                                             tCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCGGTAATAAATCCATCACTTGATTACCGTTc                        >  1:275026/1‑141 (MQ=255)
                                                                                                               aaTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACAATGTTCTAATACAATATGCTGTAATAAATCCCTCACTTGATTCACGTTCaa                      >  1:194997/1‑141 (MQ=255)
                                                                                                                aTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCaaa                     <  2:315775/141‑1 (MQ=255)
                                                                                                                 tGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAg                    <  1:336851/141‑1 (MQ=255)
                                                                                                                  gATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGc                   <  1:25569/141‑1 (MQ=255)
                                                                                                                                 tAAAACATAATTGTACTGACAACATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCAACACTTGATTACCGTTCAAAGCATCCAATGATGCAAc    <  1:388982/141‑1 (MQ=255)
                                                                                                                                  aaaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACt   <  2:329718/141‑1 (MQ=255)
                                                                                                                                   aaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTg  >  1:17357/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                    
TTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATT‑AATATTA‑CCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTG  >  CP000730/1416321‑1416590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑