Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,416,4580AT99.2% 70.0 / ‑6.8 24I216N (ATT→AAT) USA300HOU_1314oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (10/12);  minor base G (1/0);  total (12/12)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTC  >  CP000730/1416326‑1416594
                                                                                                                                    |                                                                                                                                         
aCTCAATTATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGa                                                                                                                                  <  2:158200/141‑1 (MQ=255)
     ttccttgTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAata                                                                                                                             <  2:70755/135‑1 (MQ=255)
     aTCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAata                                                                                                                             <  2:79721/141‑1 (MQ=255)
       cATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATAtt                                                                                                                           <  1:211309/141‑1 (MQ=255)
                cccTCTTTAATTGTGTTCTATATTTAATTAGAAGTCAAATAACCAGACGCAAACATACACCCTTGAATTGATTAATTTTTTATTGTTAAATTTTTTGACTTTCTTTTTAAATATATTTTTACTGACATTATTTCAATACAt                                                                                                                  >  2:93952/1‑141 (MQ=255)
                     tttAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATGGTACTGACAATATTTCAATACATGACtt                                                                                                             <  1:135257/141‑1 (MQ=255)
                     tttAATTGTGTTCTATATTTAATGAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACtt                                                                                                             <  2:4204/141‑1 (MQ=255)
                                      tttAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGataat                                                                                            <  2:280342/141‑1 (MQ=255)
                                        tAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAACAGATAAATACCACCGCGATCAATGATTTTACCTTTTTTTACAAGAAAGTGTTACGTGCAAGAGTTCAATACATGACTTACGAAAGGTGTGaaataca                                                                                          >  2:58966/1‑135 (MQ=255)
                                        tAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACCTAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGAtaataa                                                                                          <  1:9512/141‑1 (MQ=255)
                                          aTTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCATTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATaataaat                                                                                        <  1:204086/141‑1 (MQ=255)
                                                     aGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAcc                                                                             <  2:252743/141‑1 (MQ=255)
                                                           cGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTc                                                                       >  2:197952/1‑141 (MQ=255)
                                                           cGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGGTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGCGTGATAATATATAATGTATGACCATGttt                                                                       >  1:26856/1‑140 (MQ=255)
                                                                 cAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATAc                                                                 >  1:35140/1‑141 (MQ=255)
                                                                               tGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGtaata                                                   >  1:247177/1‑141 (MQ=255)
                                                                                       ctAATATTACCTCGTTCAATGATTTGCCATTCATTTAAAACATAAATGTACTAACAATATTTCAATACATGACTTAAGTTATGTGTGACAATAAAAACTGTTTGACCATGTTCTAATGCAATCTGCTGTAATAAATCTAtt                                           >  2:111397/2‑140 (MQ=255)
                                                                                         aaTATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCAc                                         <  1:44340/141‑1 (MQ=255)
                                                                                                  tCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTAcc                                >  2:174115/1‑141 (MQ=255)
                                                                                                    gTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTTTAATAAATCCATCACTTGATTACCGt                              <  2:173895/141‑1 (MQ=255)
                                                                                                          tGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAGCATGTTCTAATACAATATGCTGTAATAAATCCATCTCTTGGTTACCGTTCAAAg                        >  1:14412/1‑141 (MQ=255)
                                                                                                                     tcttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAatgat             >  1:83070/1‑141 (MQ=255)
                                                                                                                            aaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATGAACTGATTGATATCCATTACTTGATTACCGTTCAACGCATCCAATGATGCAACTg      >  1:230608/1‑141 (MQ=255)
                                                                                                                                aTAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATACAATGATGCAACTGGTTc  >  1:89487/1‑141 (MQ=255)
                                                                                                                                    |                                                                                                                                         
ACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTC  >  CP000730/1416326‑1416594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: