BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,530,723	G→A	58.5%	T188I (ACA→ATA)	USA300HOU_1396 ←	glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,530,723	0	G	A	58.5%	5.9 / 21.7	23	T188I (ACA→ATA)	USA300HOU_1396	glycosyltransferase
Reads supporting (aligned to +/- strand): ref base G (5/4); new base A (9/4); total (15/8)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

GGTATCTTTTCACGTACTTTTGCAAATGTTTCTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTGTGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCATGTGTTTCTTGTGCTAAAGATTTACTCACACTTGTCACAATATCACTTTT  >  CP000730/1530597‑1530853

ggTATCTTTTCACGTACTTTTGCAAATGTTTCTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGtt                                                                                                                      <  2:98978/141‑1 (MQ=255)

     cTTTTCACGTACTTTTGCAAATGTTTCTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGaaa                                                                                                                 <  1:23213/141‑1 (MQ=255)

           aCGTACTTTTGCAAATGTTTCTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTGTGTTATGTTTAGTTGGAAACTCAtt                                                                                                           >  2:177122/1‑141 (MQ=255)

                     gCACATGTTTCTATATCAGTATGTACGCGTTTTACTTGTCTAAATTTCGACACATTTATTAACACTTTTTCATCTCGTGCTATACCAAATTGTGATTTTAATGCTGTGTTATGTCTCGTAGGAAGCTGATTTTCACGTACa                                                                                                 <  2:199332/141‑1 (MQ=255)

                               cTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATaaa                                                                                       >  1:59941/1‑141 (MQ=255)

                                          tCTATGCGTTTTACTTGTCTAAAATTCAATACATGTATTAACACTTTTTCATCTGATGCTATACCAAATTATGATTTTAATGCTATATTATGTTTAGTTGGAAACTCATTTTCACATACAAAATTATAAAACGGAATAAtt                                                                            >  2:157895/1‑141 (MQ=255)

                                            tATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTCTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATTAATCGGTATAAtttt                                                                          >  2:14747/1‑140 (MQ=255)

                                                   tttACTTGGCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTGTGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTa                                                                   <  1:128776/141‑1 (MQ=255)

                                                         tGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCa                                                             <  1:70495/141‑1 (MQ=255)

                                                           tCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTCTAACAAATTGTGATTTTACTGCTATGTTATGTTTCGTTGGCAACTAATTATCACGTACTAAATTATAAATCGGTATATTTTCTTTGTTAGTTTCaat                                                           >  2:162664/1‑141 (MQ=255)

                                                           tCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCaat                                                           >  2:58390/1‑141 (MQ=255)

                                                           tCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCaaa                                                           >  2:44539/1‑140 (MQ=255)

                                                                      aTACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTGTGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTGAGTTTCAATAATCTCAtgtg                                                <  1:177122/141‑1 (MQ=255)

                                                                      aTACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCAtgtg                                                >  1:5752/1‑141 (MQ=255)

                                                                       tACATGTATTAACACTTTTTTTTTTTTTTTTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTTTTTGTTAGTTTCTATATTTTTATgtgt                                               >  2:33998/1‑141 (MQ=255)

                                                                        aCATGTATTAACACTTTTTTTTTTTTTTTTATACCAAATTGTGATTTTAATGCTTTGTTATGTTTAGTTGGAAACTCATTTTCACGTACACAATTATAAATCTGTATAATTTCTTTGTTAGTTTTATTTATTTTTTGTGtt                                              >  2:174313/1‑141 (MQ=255)

                                                                                tAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCATGTGTTTCTTGTGc                                      <  1:58390/141‑1 (MQ=255)

                                                                                   cacTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTGTGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCATGTGTTTCTTGTGCTaa                                   >  1:232516/1‑141 (MQ=255)

                                                                                           cATCTGGTGCTATACCAAATTGTGATTTTAATGCTGTGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCATGTGTTTCTTTTGCTAAAGATTTAc                           >  1:241567/1‑141 (MQ=255)

                                                                                                          cAAATTGTTATTTTAAGGTTGTTTTATGTTTAGGTGGTAACTTATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCATGTGTTTCTTGTGCTAAAGATTTACTCACACTTGTCACaa            <  1:213497/141‑1 (MQ=255)

                                                                                                          cAAATTGTGATTTTAATGCTGTGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCATGTGTTTCTTGTGCTAAAGATTTACTCACACTTGTCACaa            >  1:138344/1‑141 (MQ=255)

                                                                                                                  gATTTTAATGCTGTGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCATGTGTTTCTTGTGCTAAAGATTTACTCACACTTGTCACAATATAACtt    >  2:132835/1‑141 (MQ=255)

                                                                                                                    ttttAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCATGTGTTTCTTGTGCTAAAGATTTACTCACACTTGTCACAATATCACtttt  >  1:88488/1‑141 (MQ=255)

GGTATCTTTTCACGTACTTTTGCAAATGTTTCTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTGTGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCATGTGTTTCTTGTGCTAAAGATTTACTCACACTTGTCACAATATCACTTTT  >  CP000730/1530597‑1530853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`