Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,834,119 G→T 67.9% intergenic (+68/‑46) USA300HOU_1710 → / → USA300HOU_1711 hypothetical protein/class V aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,834,1190GT67.9% 17.1 / 19.5 25intergenic (+68/‑46)USA300HOU_1710/USA300HOU_1711hypothetical protein/class V aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (4/4);  new base T (10/7);  total (14/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-01

ATTTCAAATGCTGTAAGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATGAGAGAAATTCAAGCACCTATGGTTG  >  CP000730/1833989‑1834252
                                                                                                                                  |                                                                                                                                      
aTTTCAAAGCCTGTAAGAAATAATGGGTCAATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCTACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCt                                                                                                                              <  2:126341/141‑1 (MQ=255)
          cTGTAAGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTAGAGCAGATAGCATTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGa                                                                                                                    >  1:64747/1‑141 (MQ=255)
               aGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCt                                                                                                               >  1:117871/1‑141 (MQ=255)
                              aTAAAACTCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCa                                                                                                <  1:237582/141‑1 (MQ=255)
                              aTAAAAATCTATCCCATCATTCAGGCCAAATAAAGTCGTAAAGTGAAGACGTTTGTTAGACAATAAAGCGTTATCACTTTTTTAGGCATTTAAATTTTTATAAAATAGGATGAAATAGTTAAATATTATTTCTTGGAGACa                                                                                                >  2:87068/1‑141 (MQ=255)
                                  aaaTCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCAtata                                                                                            <  2:250654/141‑1 (MQ=255)
                                         ccccATCCTTCAGGCCAAATAAAATCGTTAAGGCATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATCATCATAAAATTTGTTTGCTTGGGGAAATCTTATTTATAGGAGGCATATAAATGtat                                                                                     <  1:134478/140‑1 (MQ=255)
                                           ccATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATATGAGGCATATAAATGtatta                                                                                   >  2:197960/1‑141 (MQ=255)
                                               catTCAGGGCCAATAAAATTGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTatcat                                                                               <  2:121351/141‑1 (MQ=255)
                                               catTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTatcat                                                                               <  1:279914/141‑1 (MQ=255)
                                                    aGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAAcc                                                                          >  1:224109/1‑141 (MQ=255)
                                                                  cGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAacac                                                           >  1:267053/1‑141 (MQ=255)
                                                                  cGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAacac                                                           >  1:55558/1‑141 (MQ=255)
                                                                                      tCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAGATCTTATTTATAGGAGGCATATAAGTGTATTATCATCAACGGGTGTTATTAACACCTGGCCCAACCCCTGGACCt                                       >  1:17345/1‑141 (MQ=255)
                                                                                      tCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGCGGCATATAAATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCt                                       >  1:17332/1‑141 (MQ=255)
                                                                                      tCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCt                                       >  2:219877/1‑141 (MQ=255)
                                                                                         aCAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGAt                                    <  1:244459/141‑1 (MQ=255)
                                                                                                     tATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATgaga                        <  2:64747/141‑1 (MQ=255)
                                                                                                       tCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTCCCTGATGCCATTATgagaga                      <  2:116162/141‑1 (MQ=255)
                                                                                                           ttttttAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGACATTATGAGAGAAAtt                  >  1:174518/1‑141 (MQ=255)
                                                                                                            tttttAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATGAGAGAAATTc                 >  1:18239/1‑141 (MQ=255)
                                                                                                            tttttAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATGAGAGAAATTc                 >  1:10436/1‑141 (MQ=255)
                                                                                                             ttttAGGCTTCTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATAACCTGCGTGTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATGAGAGAAATTCa                >  1:203379/1‑141 (MQ=255)
                                                                                                                   gCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATGAGAGAAATTCAAGCAcc          <  2:13313/141‑1 (MQ=255)
                                                                                                                           aTTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATGAGAGAAATTCAAGCACCTAtggttg  <  2:267053/141‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                      
ATTTCAAATGCTGTAAGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATGAGAGAAATTCAAGCACCTATGGTTG  >  CP000730/1833989‑1834252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: