Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,664,466 G→A 52.7% A65T (GCG→ACG)  USA300HOU_2518 → acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,664,4660GA52.7% ‑1.0 / 23.5 20A65T (GCG→ACG) USA300HOU_2518acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (4/5);  new base A (5/5);  total (9/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.23e-02

GCCATTATCAATACTACAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCAAAAGCTAAAGGT  >  CP000730/2664331‑2664606
                                                                                                                                       |                                                                                                                                            
gCCATTATCAATACTACAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTacgacg                                                                                                                                         <  1:275649/141‑1 (MQ=255)
   aTTATCAATACTACAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTc                                                                                                                                      >  2:38060/1‑141 (MQ=255)
      aTCAATACGACAGCTATTTATACTTATGAACCGCAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCGTAGGGTTTGCGACGTTCGGt                                                                                                                                   <  1:175620/141‑1 (MQ=255)
                 aGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGAcc                                                                                                                        <  2:134988/141‑1 (MQ=255)
                  gCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCt                                                                                                                       <  1:168074/141‑1 (MQ=255)
                   cTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCtt                                                                                                                      >  1:46618/1‑141 (MQ=255)
                                  aaCCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCtat                                                                                                       <  1:201412/141‑1 (MQ=255)
                                      acaAACCATAGACGAACGTGTAGCATAGTTTGAAACGAAACAACGTAAGAATGAGCCTATTTTTGTATTTGAGGAAAATGGAAGTATCTTAGGGTTTACGAGGTTCGGTTCATTTAGACCTTGGCCAGCATAcatgtagac                                                                                                   >  2:228805/1‑133 (MQ=255)
                                              tAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCGTTTATGCCTTGGCCGGCATCCCTATATACAATCGaaa                                                                                           >  1:6666/1‑140 (MQ=255)
                                               aGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTAGTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTCTATACAATCGAACa                                                                                          >  2:32656/1‑141 (MQ=255)
                                                     aCGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTAt                                                                                    <  1:18284/141‑1 (MQ=255)
                                                                  gttGAAAGCAAACAACTGAAGCATGAGCCTATCCTTGTATGTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTGCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCt                                                                       <  2:240905/140‑1 (MQ=255)
                                                                           aaacAACGTAAGCATGAGCCTTTCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTaga                                                              <  2:219531/141‑1 (MQ=255)
                                                                               aacGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGaa                                                          >  1:87347/1‑141 (MQ=255)
                                                                                      gCATGGGCCTATCTTTGGATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCCTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTAt                                                   <  2:144765/141‑1 (MQ=255)
                                                                                        aTGAGCCTATCTTTTTATTTGAGGAAAAGGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTACATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTg                                                 <  2:81205/141‑1 (MQ=255)
                                                                                              cTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTc                                           <  1:38060/141‑1 (MQ=255)
                                                                                                               gAAAATGGAAATGCCTTAGAGTTTACGACTTTCGGTTCATTTAGACCTTTTCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTa                          >  2:205816/1‑141 (MQ=255)
                                                                                                                  aaTGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAAtt                       >  1:123273/1‑141 (MQ=255)
                                                                                                                        aGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGaa                 >  1:236211/1‑141 (MQ=255)
                                                                                                                             cTTAGGGTTTTGGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCaaa            <  2:262332/141‑1 (MQ=255)
                                                                                                                                       gCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCAAAAGCTAAAGGt  <  1:210681/141‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                            
GCCATTATCAATACTACAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCAAAAGCTAAAGGT  >  CP000730/2664331‑2664606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: