Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 8,822 A→T 10.6% E597D (GAA→GAT)  gyrA → DNA topoisomerase (ATP‑hydrolyzing) subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007308,8220AT10.6% 101.5 / 4.8 38E597D (GAA→GAT) gyrADNA topoisomerase (ATP‑hydrolyzing) subunit A
Reads supporting (aligned to +/- strand):  ref base A (14/19);  new base T (2/2);  total (17/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

ACTTTAAGTACACATGACCATGTATTGTTCTTTACTAACAAAGGTCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAGACAACTTCTTAGTGTTTGCAACTAAACGTGGTGTCGTTAAACGTTCAGCATTAAGTAACTTCTCAAGAATAAATAGAAATGGTA  >  CP000730/8682‑8949
                                                                                                                                            |                                                                                                                               
aCTTTAAGTACACATGACCATGTATTGTTCTTTACTAACAAAGGTCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGaa                                                                                                                                 <  1:285126/141‑1 (MQ=255)
aCTTTAAGTACACATGACCATGTATTGTTCTTTACTAACAAAGGTCGTATATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGaa                                                                                                                                 <  1:46139/141‑1 (MQ=255)
            aaTGACCATGTATTGTTCTTTACTAACAAAGGTCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACa                                                                                                                     <  2:226068/140‑1 (MQ=255)
                    tggaTTGTTCTTTACTAACAAAGGGCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGCATAAGATTAAGTCATTAGTACAATGATTGc                                                                                                             <  1:284268/138‑1 (MQ=255)
                    tGTATTGTTCTTTACTAACAAAGGTCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGc                                                                                                             <  2:170404/141‑1 (MQ=255)
                    tGTATTGTTCTTTACTAACAAAGGTCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGc                                                                                                             <  2:51115/141‑1 (MQ=255)
                    tGTATTGTTCTTTACTAACAAAGGTCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTGGAAAATGATGAAGTCATTAGTACAATGATTGc                                                                                                             <  1:69341/141‑1 (MQ=255)
                          gTTCTTTACTAACAAAGGTCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTaa                                                                                                       >  2:225386/1‑141 (MQ=255)
                          gTTCTTTACTAACAAAGGTCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTAATATAACTAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTaa                                                                                                       >  1:281116/1‑141 (MQ=255)
                           ttCTTTACTAACAAAGGTCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGATGTCATTAGTACAATGATTGCTGTTaaa                                                                                                      >  2:58618/1‑141 (MQ=255)
                           ttCTTTACTAACAAAGGTCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGATGTCATTAGTACAATGATTGCTGTTaaa                                                                                                      >  2:263561/1‑141 (MQ=255)
                            tCTTGACTAACAAAGGTCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGCCAGTCTAAAGGTATTCCTGTAGTGCATGCTATTGAACTTGAAAATGATTAAGTCATTAGCACAATGATTGCTGTTAAAg                                                                                                     <  2:126689/141‑1 (MQ=255)
                             cTTTACTAACAAAGGTCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGa                                                                                                    <  2:238054/141‑1 (MQ=255)
                                 aCTAACAAAGGTCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGCTATCAAGACAGGCGAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCtt                                                                                                <  1:65850/141‑1 (MQ=255)
                                         aGGTCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGATGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGa                                                                                        <  1:263561/141‑1 (MQ=255)
                                         aGGTCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGATGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGa                                                                                        <  1:58618/141‑1 (MQ=255)
                                          ggTCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAGGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGaa                                                                                       <  2:166623/141‑1 (MQ=255)
                                           gTCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAg                                                                                      <  2:40665/141‑1 (MQ=255)
                                            tCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAGa                                                                                     >  1:166625/1‑141 (MQ=255)
                                            tCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAGa                                                                                     >  2:94164/1‑141 (MQ=255)
                                             cGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATAAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGCTGAAGTCAGAAGTACCATGCATGCTGTTAAAGACCATGAAAGTgacgac                                                                                    >  2:186314/1‑141 (MQ=255)
                                                 tataCAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGGAGACAAct                                                                                <  2:96615/141‑1 (MQ=255)
                                                    aCAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAGGACAActtct                                                                             <  2:78391/141‑1 (MQ=255)
                                                                        gTGCCTGAGATATCAAGACAGTCTCAAGGTATTCCTGTAGTGAATGCTATTGTCATTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAGACAACTTCTTAGTGTTTGCAACTAAACgg                                                         >  1:209597/1‑140 (MQ=255)
                                                                           ccTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGACTACTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAGACAACTTCTTAGTGTTTGCAACTAAACgtggt                                                      >  1:55709/1‑141 (MQ=255)
                                                                            cTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAGACAACTTCTTAGTGTTTGCAACTAAACgtggtg                                                     <  1:160658/141‑1 (MQ=255)
                                                                                  tATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAGACAACTTCTTAGTGTTTGCAACTAAACGTGGTGTCGTTa                                               <  2:166625/141‑1 (MQ=255)
                                                                                  tATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAGACAACTTCTTAGTCTTTGCAACTCAACGTGGTGTCGTTa                                               <  1:143159/141‑1 (MQ=255)
                                                                                      aacaCAGTCTAAAGGTATTCCTGTAGTCAAAGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAGACAACTTCTTAGTGTTTGCAACTAAACGTGGTGTCATTAAAca                                           <  2:2964/138‑2 (MQ=255)
                                                                                       aGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAGACAACTTCTTAGTGTTTGCAACTAAACGTGGTGTCGTTAAACGt                                          >  2:47086/1‑141 (MQ=255)
                                                                                                  aGGTATTCCTGTAGTCAATGCTATTGAACTTGAAAATGATGAAGGCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAGACAACTTCTTAGTGTTTGCAACTAAACGTGGTGTCGTTAAACGTTCAGCATTAAg                               <  1:151974/141‑1 (MQ=255)
                                                                                                    gTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAGACAACTTCTTAGTGTTTGCAACTAAACGTGGTGTCGTTAAACGTTCAGCATTAAGTa                             <  1:16812/141‑1 (MQ=255)
                                                                                                         ccTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAGACAACTTCTTAGTGTTTGCAACTAAACGTGGTGTCGTTAAACGTTCAGCATTAAGTAACTtc                        <  1:94164/141‑1 (MQ=255)
                                                                                                          cTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAGACAACTTCTTAGTGTTTGCAACTAAACGTGGTGTCGGTGAACGTTCGGCATTAAGTAACTtct                       >  1:6245/1‑141 (MQ=255)
                                                                                                           tGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGCTAAAGACCTTGAAAGTGAAGACAACTTCTTAGTGTTTGCAACTAAACGTGGTGTCGTTAACCGTTCAGCATAAAGTAACtgccc                      >  2:8176/1‑137 (MQ=255)
                                                                                                             tAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAGACAACTTCTTAGTGTTTGCAACTAAACGTGGTGTCGTTAAACGTTCAGCATTCAGTAACTTCTCaa                    >  1:13452/1‑141 (MQ=255)
                                                                                                              aGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAACTGAAGACAACTTCTTAGTGTTTGCAACTAAACGTGGTGTCGTTAAACGTTCAGCATTAAGTAACTTCTCAAg                   >  2:102005/1‑141 (MQ=255)
                                                                                                                            aaCTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAGACAACTTCTTAGTGTTTGCAACTAAACGTGGTGTCGTTAAACGTTCAGCATTAAGTAACTTCTCAAGAATAAATAGAAATg     >  1:107616/1‑141 (MQ=255)
                                                                                                                              cTTGAAAATGATGACGTCAGTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAGACAACTTCTTAGTGTTTGCAACTAAACGTGGTGTCGTTAAACGTTCAGCATTAAGTAACTTCTCAAGAATAAATAGAAATGGt   >  1:157403/1‑141 (MQ=255)
                                                                                                                               ttGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTGGAAAGTGAAGACAACTTCTTAGTGTTTGCAACTAAACGTGGTGTCGTTAAACGTTCAGCATTAAGTAACTTCTCAAGAATAAATAGAAACGGTa  >  2:252818/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                               
ACTTTAAGTACACATGACCATGTATTGTTCTTTACTAACAAAGGTCGTGTATACAAACTTAAAGGTTACGAAGTGCCTGAGTTATCAAGACAGTCTAAAGGTATTCCTGTAGTGAATGCTATTGAACTTGAAAATGATGAAGTCATTAGTACAATGATTGCTGTTAAAGACCTTGAAAGTGAAGACAACTTCTTAGTGTTTGCAACTAAACGTGGTGTCGTTAAACGTTCAGCATTAAGTAACTTCTCAAGAATAAATAGAAATGGTA  >  CP000730/8682‑8949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑