Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 707,879 A→T 100% L11I (TTA→ATA)  USA300HOU_0653 ← ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730707,8790AT100.0% 82.1 / ‑6.5 28L11I (TTA→ATA) USA300HOU_0653ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (8/19);  total (9/19)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAA  >  CP000730/707748‑708012
                                                                                                                                   |                                                                                                                                     
aGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGAtt                                                                                                                              >  2:42150/1‑141 (MQ=255)
aGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGACCCATGATACCAATTATTTCGCCGCGTACAGGTATCGATAAGGAAATGGTATTAAGTACATGCTTATTACCTATAAACAGAtt                                                                                                                              >  2:157441/1‑141 (MQ=255)
 gCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGAttt                                                                                                                             <  1:231072/141‑1 (MQ=255)
      aaaTTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATc                                                                                                                        <  1:45169/141‑1 (MQ=255)
             ccAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGtt                                                                                                                 <  2:96502/141‑1 (MQ=255)
              cAATTGAGGAGTGGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGttt                                                                                                                <  2:119710/141‑1 (MQ=255)
                      gACTTGATGAGCGACGATTTATCAGGTACATCCGGGCCCATGATTCCTATTATTTCGACGCGTACTGGTATCAGTAAGGAAATGTTTTGAAGTACATGCTTATTACCTATAAATAGATTTAATTCTTTTGTTTCTAACaaa                                                                                                        <  2:15479/141‑1 (MQ=255)
                        cctGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTCGTTTCTAACAAACg                                                                                                      <  1:187161/139‑1 (MQ=255)
                          tGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGtt                                                                                                    >  1:218305/1‑141 (MQ=255)
                          tGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGtt                                                                                                    <  1:84434/141‑1 (MQ=255)
                          tGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTGTTAAGTACAGACGTTTTACCTATAATCATAGTTATATCTTCTGTTTCTAACAAACttt                                                                                                    >  1:274070/1‑141 (MQ=255)
                                        ttACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAAtt                                                                                      <  1:155597/141‑1 (MQ=255)
                                             aGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGAGTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAg                                                                                 >  1:267687/1‑141 (MQ=255)
                                                                         attTCGACGCGTAGTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTataat                                                     <  2:135361/141‑1 (MQ=255)
                                                                              gccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACt                                                >  2:221056/1‑141 (MQ=255)
                                                                               ccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTg                                               >  2:272422/1‑141 (MQ=255)
                                                                                       tGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATAtt                                       <  1:263392/141‑1 (MQ=255)
                                                                                           aTCGATAAGGAAATGTTTTAAAGAACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATc                                   <  2:273249/141‑1 (MQ=255)
                                                                                              gctAAAGAAATGTATTTCAGTGCTGTCGTAGTGCCTAAAAACGTATTTGAATCTGTTGTTGCTAACAAACGTTTATACCTCCTAGTTAAAAGTTTAGGCTAACCTAATGAATTGTACAATAAACTGATAATATTTATCATg                                >  2:167163/3‑141 (MQ=255)
                                                                                                   gtaaaGGTTTTTAACTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                           <  1:10856/139‑1 (MQ=255)
                                                                                                   ggAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                           <  2:126150/141‑1 (MQ=255)
                                                                                                     aaaTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                         <  2:169921/141‑1 (MQ=255)
                                                                                                     aaaTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGGTTATACCTCCTAATTAAAAGTTTAGGCGAACCTAATTAATTGTATAATAAACTGAGAATATGTATCATGTCAAGTa                         <  1:42150/141‑1 (MQ=255)
                                                                                                     aaaTGTTTTTAAGGACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGTATATTTATCATGTCAAGTa                         <  1:157441/141‑1 (MQ=255)
                                                                                                      aaTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaa                        <  2:191271/141‑1 (MQ=255)
                                                                                                                        cTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAg      <  1:49209/141‑1 (MQ=255)
                                                                                                                        cTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAg      >  1:131777/1‑141 (MQ=255)
                                                                                                                            ttaCCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATACTAAACCGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACaa  <  2:33984/141‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                     
AGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAA  >  CP000730/707748‑708012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑