| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,064,126 | G→C | 100% | Y121* (TAC→TAG) | USA300HOU_1000 ← | LytR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,064,126 | 0 | G | C | 100.0% | 68.1 / NA | 21 | Y121* (TAC→TAG) | USA300HOU_1000 | LytR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (11/10); total (11/10) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCTCGACAAATGCTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTC > CP000730/1064005‑1064253 | ccTCGACAAATGCTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCCTAATAGCCAACTTTAGGaa < 2:256032/141‑1 (MQ=255) cGACAAATGCTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACTACCATATGCATGTGCATGGGTAATCTTATCCTAATAGCCAACTTTAGGAATGt < 2:87535/141‑1 (MQ=255) aCAAATGCTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCCTAATAGCCAACTTTAGGAATGTAg > 2:7630/1‑141 (MQ=255) aCAAATGCTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCCTAATAGCCAACTTTAGGAATGTAg > 2:178722/1‑141 (MQ=255) tGCTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCCTAATAGCCAACTTTAGGAATGTAGCTGAt > 1:187681/1‑141 (MQ=255) cATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCCTAATAGCCAACTTTAGGAATGTAGCTGATAGTATc > 1:282073/1‑141 (MQ=255) tatCGACTCTCACATAATAAGCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCCTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCAc < 1:40432/141‑1 (MQ=255) acaTAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCCTACTAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTa > 1:62956/1‑141 (MQ=255) gTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCCTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGAt < 1:192917/141‑1 (MQ=255) ctGGTAGCTTCTATCGAATCCATTGCGGCCATTGGACCACCATATGCATGTGCATGGGTTATCTTATCCTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTttgat < 2:285841/140‑1 (MQ=255) ggTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCCTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTttgattg > 1:120736/1‑141 (MQ=255) gAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCCTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATCCTAATTTGATGTTTTGATTGATTAAAAGTAGtt < 2:265420/141‑1 (MQ=255) ttGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCCTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCa > 1:169120/1‑141 (MQ=255) accaTATGCATGTGCATGGGTAATCTTATCCTAATAGCGATCTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCGACCTAATTTTTTGTTTTGATTGATTGAAAGTAGCTAAATTCACAGCGTCTGATCTaga < 2:116459/141‑1 (MQ=255) tatGCATGTGCATGGGTAATCTTATCCTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATcacgaatgc > 2:213045/1‑133 (MQ=255) atGCATGTGCATGGGTAATCTTATCCTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGtt > 1:81472/1‑141 (MQ=255) gtgcatgGGTAATCTTATCCTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCAt > 1:244193/1‑141 (MQ=255) catgGGTAATCTTATCCTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGGGTGTTCAGCATCCt > 2:225338/1‑141 (MQ=255) tgGGTAATCTTATCCTAATAGCCAACTTTATGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTACATTGTTCAGCATCCTGt < 1:225003/141‑1 (MQ=255) tgGGTAATCTTATCCTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGt < 1:166330/141‑1 (MQ=255) gggTAATCTTATCCTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTc < 1:152436/141‑1 (MQ=255) | CCTCGACAAATGCTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTC > CP000730/1064005‑1064253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |