| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,238,307 | C→T | 100% | Q141* (CAA→TAA) | USA300HOU_1162 → | glycerone kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,238,307 | 0 | C | T | 100.0% | 52.6 / NA | 18 | Q141* (CAA→TAA) | USA300HOU_1162 | glycerone kinase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (11/7); total (11/7) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTAAAAATATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGCAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGTGTTGTT > CP000730/1238170‑1238438 | gTAAAAATATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAg > 2:46723/1‑141 (MQ=255) ttGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAg > 1:117193/1‑141 (MQ=255) tGAAAGTGAATCTGATATTAATTCAAGATTGTTATCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGCAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGa < 1:173823/141‑1 (MQ=255) ttaattCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATAcag > 2:146101/1‑139 (MQ=255) ttaattCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAACCTGCAATAGAAAAAGCAAATAATACTg > 2:144982/1‑141 (MQ=255) aattCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACGGaa < 1:26391/141‑1 (MQ=255) aTTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCCATAGAAAAAGCAAATAATACTGAAGATTGtat < 1:222533/141‑1 (MQ=255) ttGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGtata < 2:284887/141‑1 (MQ=255) tCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACat > 1:188056/1‑141 (MQ=255) tgtTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTaaa > 2:248461/1‑141 (MQ=255) gtTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTaaaa < 1:132080/141‑1 (MQ=255) ggCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGa > 1:282135/1‑141 (MQ=255) cATATAAAGCTGTTATGAAACCAGTCGACAGTACAATACTTACAATGGCAAAAGATGCTACGTAAGCTGCAATAGAAACGACAACAACGCCGGAACCACGTCTAGAATGAATGGAGGAAATTATTGTaaagagcgatgcac > 2:104441/1‑130 (MQ=255) ttGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACttat > 2:169033/1‑141 (MQ=255) tACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTa < 1:46723/141‑1 (MQ=255) aaGATGCTGCGTAAGCTGCAATAGAAAAAGCAAGTAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGtgttt > 1:13576/1‑140 (MQ=255) gATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGTgttgtt < 1:548/141‑1 (MQ=255) gATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGTgttgtt > 2:548/1‑141 (MQ=255) | GTAAAAATATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGCAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGTGTTGTT > CP000730/1238170‑1238438 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |