| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,345,220 | A→G | 18.8% | intergenic (+32/‑18) | USA300HOU_1256 → / → USA300HOU_1257 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,345,220 | 0 | A | G | 18.8% | 66.6 / 9.7 | 32 | intergenic (+32/‑18) | USA300HOU_1256/USA300HOU_1257 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (13/13); new base G (3/3); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
ATGAATTCGAGAGCGAAACAGGAAATCTTGTTGTAGATATACAGACTGATTTTGCTGTTTATTCGTTTGATGAAACTGAGATAAAAAGTATTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATTTTATGCACAAATGAATATAATAATAAGAAAAATAATTCATCTTTATTACCAAGTTATACTAT > CP000730/1345082‑1345359 | aTGAATTCGAGAGCGAAACAGGAAATCTTGATGTAGATATACAGACTGATTTTGCTGTTTATTCGTTTGATGAAACTGAGATAAAAAGTATTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGATaat > 2:174293/1‑140 (MQ=255) ttCGAGAGCGAAACAGGAAATCTTGTTGTAGATATACAGACTGATTTTGCTGTTTATTCGTTTGATGAAACTGAGATAAAAAGTATTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGa > 1:95341/1‑141 (MQ=255) agagCGAAACAGGAAATCTTGTTGTAGATATACAGACTGATTTTGCTGTTTATTCGTTTGATGAAACTGAGATAAAAAGTATTAGATTATTAAAATAATTACAAAATAGCATACTTTCTACAAAAAGCTAAAAAAGAAATg > 1:146002/1‑141 (MQ=255) aaCAGGAAATCTTGTTGTAGATATACAGACTGATTTTGCTGTTTATTCGTTTGATGAAACTGAGATAAAAAGTATTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTGAAAAAGAAATGCTTGaaa > 1:220266/1‑141 (MQ=255) aaCAGGAAATCTTGTTGTAGATATACAGACTGATTTTGCTGTTTATTCGTTTGATGAAACTGAGATAAAAAGTATTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTGAAAAAGAAATGCTTGaaa > 1:25135/1‑141 (MQ=255) aaCAGGAAATCTTGTTGTAGATATACAGACTGATTTTGCTGTTTATTCGTTTGATGAAACTGAGATAAAAAGTATTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTGAAAAAGAAATGCTTGaaa > 1:226513/1‑141 (MQ=255) aGGATATCTTGTTGTAGATATACAGACTGATTTTGCTGTTTATTCGTTTGATGAAACTGAGATAAAAAGTATTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGa < 2:181045/141‑1 (MQ=255) aaaTCTTTTTGTAGATATACCGACTGATTTTGCTGTTTATTCGTTTGATGAAACTGAGATAAAAAGTATTAGATTATTAAAAGAATTACAAAATAGCATCCTTTCTACAAAAAGCTACAAAAGAAATGCTTGAAATGATaa > 1:180794/1‑141 (MQ=255) gATATACAGACTGATTTTGCTGTTTATTCGTTTGATGAAACTGAGATAAAAAGTATTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTaaaaa > 2:279511/1‑140 (MQ=255) ttttGCTGTTTATTCGTTTGATGAAACTGAGATAAAAAGTATTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGa > 2:114339/1‑141 (MQ=255) tttGCTGTTTATTCGTTTGATGAAACTGAGATAAAAAGTATTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGaa < 2:252024/141‑1 (MQ=255) ttGCTGTTTATTCGTTTGATGAAACTGAGAAAAAAAGTATTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGaat < 1:225871/141‑1 (MQ=255) tgaAACTGAGATAAAAAGTATTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTc < 1:233951/141‑1 (MQ=255) tgaAACTGAGATAAAAAGTATTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTc < 1:170289/141‑1 (MQ=255) aCTGAGATAAAAAGTATTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAACGGCagat > 1:103006/1‑141 (MQ=255) aCTGAGATAAAAAGTATTAGATTATTAAAATAATTACAAAATAGCATCCGTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCatat > 1:151048/1‑141 (MQ=255) aTAAAAAGTATTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATTTTATg < 2:80372/141‑1 (MQ=255) aaaaaaGTCTTGTATTATTAAAATAATTAAAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATTTTATGc < 2:190999/140‑1 (MQ=255) tATTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATTTTATGCACAAATg > 2:158301/1‑141 (MQ=255) aTTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATTTTATGCACAAATGa < 1:270974/141‑1 (MQ=255) aTTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATTTTATGCACAAATGa < 1:158301/141‑1 (MQ=255) attaAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATTTTATGCACACATGAATataata > 2:209747/1‑141 (MQ=255) taatTACAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATTTTATGCACAAATAAATATAACAATACGaa > 2:147853/1‑141 (MQ=255) aCAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATTTTATGCACAAATGAATATAATAATAAGAAAaata > 2:143841/1‑141 (MQ=255) aCAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATGAGATGCACAAATGAATATAATAATAAGAAAaata > 1:193762/1‑141 (MQ=255) aaaTAGCATCCTTTCTACAAAAAGCTGAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATTTTATGCACAAATGAATATAATAATAAGAAAAATAAtt < 2:226513/141‑1 (MQ=255) aaaTAGCATCCTTTCTACAAAAAGCTGAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATTTTATGCACAAATGAATATAATAATAAGAAAAATAAtt < 2:25135/141‑1 (MQ=255) aaaTAGCATCCTTTCTACAAAAAGCTGAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATTTTATGCACAAATGAATAAAAAAATAAGAAAAATAAtt < 2:220266/141‑1 (MQ=255) aaTAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATTTTATGCACAAATGAATATAATAATAAGAAAAATAAtta > 2:35301/1‑140 (MQ=255) ttCTACAAAAAGTTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATTTTATGCACAAATGAATATAATAATAAGAAAAATAATTCATCTTTATTAc < 1:120334/141‑1 (MQ=255) cTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATTTTATGCACAAATGAATATAATAATAAGAAAAATAATTCATCTTTATTACCAAGTTATACta < 1:286816/141‑1 (MQ=255) tAAAAAAGAAATGCTTGAAATGATAAGTAATAGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATTTTATGCACAAATGAATATAATAATAAGAAAAATAATTCATCTTTATTACCAAGTTATACtat < 1:30070/141‑1 (MQ=255) tAAAAAAGAAATGCTTGAAATGATAAGGAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATTTTATGCACAAATGAATATAATAATAAGAAAAATAATTCATCTTTATTACCAAGTTATACtat < 2:95341/141‑1 (MQ=255) | ATGAATTCGAGAGCGAAACAGGAAATCTTGTTGTAGATATACAGACTGATTTTGCTGTTTATTCGTTTGATGAAACTGAGATAAAAAGTATTAGATTATTAAAATAATTACAAAATAGCATCCTTTCTACAAAAAGCTAAAAAAGAAATGCTTGAAATGATAAGTAATGGTAAAATGACGATGAAATTGAATAATGTTAAGCAAAAGCGTCATATTTTATGCACAAATGAATATAATAATAAGAAAAATAATTCATCTTTATTACCAAGTTATACTAT > CP000730/1345082‑1345359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |