Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,555,359 A→G 13.8% intergenic (+55/+51) fer → / ← USA300HOU_1423 ferredoxin/hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,555,3590AG13.8% 63.7 / 3.7 29intergenic (+55/+51)fer/USA300HOU_1423ferredoxin/hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base A (11/14);  new base G (2/2);  total (13/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

GAAGATGCAATTGATGGATGCCCTACAGATTCTATTAAAATTGCAGACGAATCATTTGATGGGGACGCTTTAAAATTTGAATAATTGATTTTTTAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTTTATTATTATGTGTATATTATTTTGCTTTAATTTGATTAAATTCTTTTCAAGAAATTCGCAAGCCTTCTATATAGTAAAATAAATACAATAGAAATAACGATACCTTTAATAAT  >  CP000730/1555221‑1555487
                                                                                                                                          |                                                                                                                                
gAAGATGCAATTGATGGATGCCCTACAGATTCTATTAAAATTGCAGACGAATCATTTGATGGGGACGCTTTAAAATTTGAATAATTGATTTTTTAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATa                                                                                                                                <  1:128568/141‑1 (MQ=255)
 aaGATGCAATTGATGGATGCCCTACAGATTCTATTAAAATTGCAGACGAATCATTTGATGGGGACGCTTTAAAATTTGAATAATTGATTTTTTAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGGTAg                                                                                                                               >  1:154842/1‑141 (MQ=255)
 aaGATGCAATTGATGGATGCCCTACAGATTCTATTAAAATTGCAGACGAATCATTTGATGGGGACGCTTTAAAATTTGAATAATTGATTTTTTAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGGTAg                                                                                                                               >  1:203540/1‑141 (MQ=255)
               ggatgCCCTACAGATTCTATTAAAATTGCAGACGAATCATTTGATGGGGACGCTTTAAAATTTGAATAATTGATTTTTTAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTtta                                                                                                                 <  2:229558/141‑1 (MQ=255)
                  tgtcttACAGATTCTATTAAAATTGCAGACGAATCATTTGATGGGGACGCTTTAAAATTTGAATAATTGATTTTTTAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTttatta                                                                                                              <  2:99127/136‑1 (MQ=255)
                           gctccTATTAAAATTGCAGACGAATCATTTGATGGGGACGCTTTAAAATTTGAATAATTGATTTTTAAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTTTATTATTATGTGta                                                                                                     <  2:213249/137‑1 (MQ=255)
                           gATTCTATTAAAATTGCAGACGAATCATTTGATGGGGACGCTTTAAAATTTGAATAATTGATTTTTTAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTTTATTATTATGTGta                                                                                                     <  2:45010/141‑1 (MQ=255)
                                       aTTGCAGACGAATCATTTGATGGGGACGCTTTAAAATTTGAATAATTGATTTTTTAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTTTATTATTATGTGTATATTATTTTGCt                                                                                         <  1:35063/141‑1 (MQ=255)
                                            aGACGAATCATTTGATGGGGACGCTTTAAAATTTGAATAATTGATTTTTTAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTTTATTATTATGTGTATATTATTTTGCTTTAAt                                                                                    >  1:38953/1‑141 (MQ=255)
                                                       ttGATGGGGACGCTTTAAAATTTGAATAATTGATTTTTTAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATAGTAACCTGCTTTTTATTATTATGTGTATATTATTTTGCTTTAATTTGTTTAAAtt                                                                         >  2:133830/1‑141 (MQ=255)
                                                             gggACGCTTTAAAATTTGAATAATTGATTTTTTAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGGTAGTCACCTGCTTTTTATTATTATGTGTATATTATTTTGCTTTAATTTGATTAAATTCTTTTc                                                                   <  2:154842/141‑1 (MQ=255)
                                                             gggACGCTTTAAAATTTGAATAATTGATTTTTTAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGGTAGTCACCTGCTTTTTATTATTATGTGTATATTATTTTGAGTTAAGTTGATTAAATTCTTTTc                                                                   <  2:203540/141‑1 (MQ=255)
                                                               gACGCTTTAAAATTTGAATCATTGATTTTTTAGACCACTGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTTTATTATTATGTGTATATTATTTTGCTTTAATTTGATTAAATTCTTTTCaa                                                                 >  1:42782/1‑141 (MQ=255)
                                                               gACGCTTTAAAATTTGAATAATTGATTTTTTAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTTTATTATTATGTGTATATTATTTTGCTTTAATTTGATTAAATTCTTTTCaa                                                                 >  1:32759/1‑141 (MQ=255)
                                                                  gCTTTAAGATTTGAATAATTGATTGTTTAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTTTATTATTATGTGTATATTATTTTGCTTTAATTTGATTAAATTCTTTTCAAGaa                                                              >  1:182293/1‑141 (MQ=255)
                                                                  gCTTTAAAATTTGAATAATTGATTTTTTAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTTTATTATTATGTGTATATTATTTTGTTTTTATTTGATTAAATTGTTTTTAAGaa                                                              >  1:193148/1‑141 (MQ=255)
                                                                        aaaTTTGAATAAGTGATTTTTTAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTTTATTATTATGTGTATATTATTTTGCTTTAATTTGATTAAATTCTTTTCAAGAAATTCGc                                                        <  2:251714/141‑1 (MQ=255)
                                                                           tttGAATAATTGCTTTTTTAGACAACAGTTCTATAGAAAAAGCAGCCAAGCTATAGTTTATTGATAGTCACCTGCTTTTTATTATTATCTGTATATAATTTTGCTTTAATTTGATTAAATACTCTTAATGAACTTCGcccg                                                     >  1:273029/1‑138 (MQ=255)
                                                                                  aatTGATTTTTTAGACAAATGTTCTATAAAAAAAGCAGGCAAGCTATCGTTGATTGAAAGTAACATAATATTTATCATTATGTGTATATTATTTTGCCTTAATTTGATTAAATTCTTTTCAAGAAATTCGCAAgaaaaaaa                                              <  2:28943/141‑8 (MQ=255)
                                                                                   atTGATTTTTTAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTTTATTATTATGTGTATATTATTTTGCTTTAATTTGATTAAATTCTTTTCAAGAAATTCGCAAGCCTTatat                                             >  1:167817/1‑141 (MQ=255)
                                                                                         tttttAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTTTATTATTATGTGTATATTATTTTGCTTTAATTTGATTAAATTCTTTTCAAGAAATTCGCAAGCCTTCTATATAGTa                                       <  2:27258/141‑1 (MQ=255)
                                                                                          ttttAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTTATAGTCACCTGCTTTTTATTATTATGTGTATATTATTTTGCTTTAATTTGATTAAATTCTTTTCAAGAAATTCGCAAGCCTTCTATATAGTaa                                      <  2:42782/141‑1 (MQ=255)
                                                                                          ttttAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTTTATTATTATGTGTATATTATTTTGCTTTAATTTGATTAAATTCTTTTCAAGAAATTCGCAAGCCTTCTATATAGTaa                                      <  2:32759/141‑1 (MQ=255)
                                                                                            ttAGTCATATGTTCTATAGAAAAAGCAGTCAAGCTATCGTTTATTGATATTCACCTGCTTTTTATTATTATGTGTATATTATTTTGCTTTAATTTGATTAAATTCTTTTCAAGAATTTCGCAAGCCTTCTATATAGTaaaa                                    <  1:143180/141‑1 (MQ=255)
                                                                                             tAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTTTATTATTATGTGTATATTATTTTGCTTTAATTTGATTAAATTCTTTTCAAGAAATTCGCAAGCCTTCTATATAGTaaaaa                                   >  1:99400/1‑140 (MQ=255)
                                                                                                     gtttCTATAGAAAAAGCAGTAAAGCTATCGTTTATTGATAGTCACCTCCTTTTTACTATTATGTGTATATTATTTTGCTTTAATTTGATTAAATTCTTTTCAAGAAATCCGCAAGCCTTCTATATAGTAAAATAAATACaa                           <  2:118085/139‑1 (MQ=255)
                                                                                                                     cAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTTTATTTTTATGTGTATATTATTTTGCTTTAATTTGATTAAATTCTTTTCAAGAAATTCGCAAGCCTTCTATATAGTAAAATAAATACAATAGAAATAACGATAcc           >  2:214705/1‑141 (MQ=255)
                                                                                                                     cAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTTTATTATTATGTGTATATTATTTTGCTTTAATTTGATTAAATTCTTTTCAAGAAATTCGCAAGCCTTCTATATAGTAAAATAAATACAATAGAAATAACGATAcc           >  2:214822/1‑141 (MQ=255)
                                                                                                                              tATCGTTTATTGATAGTCACCTGCTTTTTATTATTATGTGTATATTATTTTGCTTTAATTTGATTAAATTCTTTTCAAGAAATTCGCAAGCCTTCTATATAGTAAAATAAATACAATAGAAATAACGATACCTTTaataat  <  1:26426/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                
GAAGATGCAATTGATGGATGCCCTACAGATTCTATTAAAATTGCAGACGAATCATTTGATGGGGACGCTTTAAAATTTGAATAATTGATTTTTTAGACAAATGTTCTATAGAAAAAGCAGGCAAGCTATCGTTTATTGATAGTCACCTGCTTTTTATTATTATGTGTATATTATTTTGCTTTAATTTGATTAAATTCTTTTCAAGAAATTCGCAAGCCTTCTATATAGTAAAATAAATACAATAGAAATAACGATACCTTTAATAAT  >  CP000730/1555221‑1555487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑