Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,644,319 C→T 8.7% K72K (AAG→AAA)  accB ← biotin carboxyl carrier subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,644,3190CT 8.7% 123.4 / 2.3 46K72K (AAG→AAA) accBbiotin carboxyl carrier subunit
Reads supporting (aligned to +/- strand):  ref base C (26/16);  new base T (2/2);  total (28/18)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

TGCCTCTAAAATACACACTGTTGTTTCATTTGAAACAGTGTCCCCAACTTGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCATTTGTGAGATTTGTGGCGTGATAATCTCAGTTTCTTTTTCTTTCTTAAGCGTCACTTTGCCTTTAG  >  CP000730/1644181‑1644458
                                                                                                                                          |                                                                                                                                           
tGCCTCTAAAATACACACTGTTGTTTCATTTGAAACAGTGTCCCCAACTTGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAATCGGCtt                                                                                                                                           >  2:178563/1‑141 (MQ=255)
tGCCTCTAAAATACACACTGTTGGTTCATTTGAAACAGTGTCCCCAACTTGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCtt                                                                                                                                           <  2:200486/141‑1 (MQ=255)
   ctctAAAATACACACTGTTGTTTCATTTGAAACAGTGTCCCCAACTTGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTgg                                                                                                                                        >  2:276036/1‑141 (MQ=255)
    tctAAGCTACACACTGTTGTTTCATTTGAAACAGTGTCCCCAACTTGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGa                                                                                                                                       <  2:64698/141‑1 (MQ=255)
     ctAAAATACACACTGTTGTTTCATTTGAAACAGTGTCCCCAACTTGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAg                                                                                                                                      >  1:250515/1‑141 (MQ=255)
     ctAAAATACACACTGTTGTTTCATTTGAAACAGTGTCCCCAACTTGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAg                                                                                                                                      >  1:73997/1‑141 (MQ=255)
       aaaaTACACACTGTTGTTTCATTTGAAACAGTGTCCCCAACTTGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCCTTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCt                                                                                                                                    >  1:101212/1‑141 (MQ=255)
          aTACACACTGTTGTTTCATTTGAAACAGTGTCCCCAACTTGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTTATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCa                                                                                                                                 >  1:139430/1‑141 (MQ=255)
                      gttTCATTTGAAACAGTGTCCCCAACTTGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTa                                                                                                                     >  1:50404/1‑141 (MQ=255)
                           aTTTGAAACAGTGTCCCCAACTTGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTGTCAGt                                                                                                                >  2:37049/1‑141 (MQ=255)
                                    aGTGTCCCCAACTTGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGc                                                                                                       >  1:259771/1‑141 (MQ=255)
                                     gtgtCCCCAACTTGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCt                                                                                                      >  2:33376/1‑141 (MQ=255)
                                     gtgtCCCCAACTTGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCt                                                                                                      >  1:156166/1‑141 (MQ=255)
                                        tCCCCAACTTGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGa                                                                                                   >  1:270996/1‑141 (MQ=255)
                                         ccccACCTTGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGag                                                                                                  <  2:109941/141‑1 (MQ=255)
                                         ccccAACTTGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGag                                                                                                  >  2:75928/1‑141 (MQ=255)
                                                 tGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATGAAATGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGaggcataggc                                                                                          >  2:141797/1‑141 (MQ=255)
                                                   cacaTATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCCTCGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTCGCTTGTGCTTGtggcctatgcgt                                                                                        >  1:232684/1‑139 (MQ=255)
                                                    acaTATGCTTGTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGTTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCAtt                                                                                       >  1:207823/1‑141 (MQ=255)
                                                    acaTATGCTTATTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTACTTGTTTTGTGATTATCTGAAGTTGGTTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCAtt                                                                                       >  1:207836/1‑141 (MQ=255)
                                                             tcttcGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCt                                                                              <  2:163979/141‑1 (MQ=255)
                                                                  gTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAAc                                                                         >  2:69802/1‑141 (MQ=255)
                                                                  gTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGAATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAAc                                                                         >  2:228216/1‑141 (MQ=255)
                                                                   tCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGATCTCAGGTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACt                                                                        <  1:55755/141‑1 (MQ=255)
                                                                       gagaTGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCa                                                                    <  2:156166/141‑1 (MQ=255)
                                                                        agaTGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCAt                                                                   >  1:285306/1‑141 (MQ=255)
                                                                           tGGCGATTTGTAAAATGTACCTACCATAGGTGCATTCATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTGTTATTGCTATCAGGTGATGGTGCTTGAGGCATAGGCATCGCCGCAGCTTCAACTGGCATTtg                                                                >  1:126917/1‑141 (MQ=255)
                                                                            ggCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATCGGCATTGCCGCAGCTTCAACTGGCATTtgt                                                               >  2:194842/1‑141 (MQ=255)
                                                                            ggCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCACTtgg                                                               >  2:80062/1‑140 (MQ=255)
                                                                                     tAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGAGTATCTGAAGTTGGCGTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCATTTGTGAGATTtgt                                                      <  1:21232/141‑1 (MQ=255)
                                                                                         aTGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCATTTGTGAGATTTGTGGCg                                                  <  2:50404/141‑1 (MQ=255)
                                                                                                 accATAGGTGCATTAAGTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCATGAGGCATAGGCATTGCCGCAGCTTCGACTGGCATTTGTGAGATTTGTGGCGTGATAAtc                                          >  1:147426/1‑141 (MQ=255)
                                                                                                     tAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCATTTGTGAGATTTGTGGCGTGATAATCTCAg                                      <  2:176098/141‑1 (MQ=255)
                                                                                                                aTTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCATTTGTGAGATTTGTGGCGTGATAATCTCAGtttctttttat                           >  2:161762/1‑139 (MQ=255)
                                                                                                                     tttGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCATTTGTGAGATTTGTGGCGTGATAATCTCAGTTTCTTTTtctttctt                      <  2:73997/141‑1 (MQ=255)
                                                                                                                            ttATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCATTTGTGAGATTTGTGGCGTGATAATCTCAGTTTCTTTTTCTTTCTTAAGCGTc               >  1:95274/1‑141 (MQ=255)
                                                                                                                              ttCTGAAGTTGGCTTTGGAGCTTCAGTGTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCATTTGTGAGATTTGTGGCGTGATAATCTCAGTTTCTTTTTCTTTCTTAAGCGTCAc             <  2:126917/140‑1 (MQ=255)
                                                                                                                                 tGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCATTTGTGAGATTTGTGGCGTGATAATCTCAGTTTCTTTTTCTTTCTTAAGCGTCACttt          >  1:232551/1‑141 (MQ=255)
                                                                                                                                 tGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCATTTGTGAGATTTGTGGCGTGATAATCTCAGTTTCTTTTTCTTTCTTAAGCGTCACttt          <  2:21636/141‑1 (MQ=255)
                                                                                                                                   gaGTTGGCCTTGGAGCTTCAGCTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCATTTGTGAGATTTGTGGCGTGATAATCTCAGTTTCTTTTTCTTTCTTAAGCGTGACTTTGc        <  1:178563/140‑1 (MQ=255)
                                                                                                                                    ggTTGGCTTTGGAGCTTCATTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCATTTGTGAGATTTGTGGCGTGATAATCTCAGTTTCTTTTTCTTTCTTAAGCGTCACTTTGcc       <  2:132319/140‑1 (MQ=255)
                                                                                                                                     gTTGGCTTTGGAGCTTCCGTTGTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCATTTGTGAGATTTGTGGCGTGATAATCTCAGTTTCTTTTTCTTTCTTAAGCGTCACTTTGCCt      <  1:145645/141‑1 (MQ=255)
                                                                                                                                     gTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCATTTGTGAGATTTGTGGCGTGATAATCTCAGTTTCTTTTTCTTTCTTAATCTTCACTTTGCCt      >  1:250460/1‑141 (MQ=255)
                                                                                                                                       tGGTTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCATCTTCAACTGGCATTTGTGAGATTTGTGGCGTGATAATCTCAGTTTCTTTTTCTTTCTTAAGCGTCACTTTGCCttt    <  2:207823/141‑1 (MQ=255)
                                                                                                                                       tGGTTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCATTTGTGAGATTTGTGGCGTGATAATCTCAGTTTCTTTTTCTTTCTTAAGCGTCACTTTGCCttt    <  2:207836/141‑1 (MQ=255)
                                                                                                                                         gCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTTCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCATTTGTGAGATTTGTGGCGTGATAATCTCAGTTTTTTTTTCTTTCTTAAGTGTCACTTTGCCTTTAg  <  1:141797/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
TGCCTCTAAAATACACACTGTTGTTTCATTTGAAACAGTGTCCCCAACTTGCACATATGCTTCTTCGTCTGGAGATGGCGATTTGTAAAATGTACCTACCATAGGTGCATTAATTGTTTTGTGATTATCTGAAGTTGGCTTTGGAGCTTCAGTTTTATTGCTATCAGTTGATTGTGCTTGAGGCATAGGCATTGCCGCAGCTTCAACTGGCATTTGTGAGATTTGTGGCGTGATAATCTCAGTTTCTTTTTCTTTCTTAAGCGTCACTTTGCCTTTAG  >  CP000730/1644181‑1644458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑