| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,797,772 | G→C | 100% | R7G (CGC→GGC) | USA300HOU_1679 ← | sensor histidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,797,772 | 0 | G | C | 98.1% | 61.6 / ‑6.4 | 21 | R7G (CGC→GGC) | USA300HOU_1679 | sensor histidine kinase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base C (10/10); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCGGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTACATCAACAATT > CP000730/1797661‑1797907 | cTAGAGCTTGTTCGTGTTTTTCTGATCAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATCAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAgg < 1:174972/141‑1 (MQ=255)cTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAgg < 1:272703/141‑1 (MQ=255) tatagCTTGTTCGTGTTTTTCTGATAAAACATATGTTATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGt < 1:6747/138‑1 (MQ=255) ttGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTcc < 1:75983/141‑1 (MQ=255) ttttCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATAATTGTTCTTTAGGTCTTTCCAATTTATAGc > 1:110171/1‑141 (MQ=255) ttCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATGATTAATATACTGCTTATCAATAACATTAAACGGTGATGAAAGTTAATCATTGTTATTTAAGCCATCGCAAGTTATAGCCt > 2:115617/1‑141 (MQ=255) tAAAACCTACGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTATGTCTTTCCAATTTATAACCTAAGCCa > 2:232947/1‑141 (MQ=255) aaaaCATATGTGATTGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGCGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCAc < 1:192447/141‑1 (MQ=255) aTGGAAGCTTGAATAATACTACCTAATCGAACAATACTGATACTTAATATACTGCTTATCAATAACATTAAGTCGTCGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTtaa < 2:273664/141‑1 (MQ=255) aTGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTtaa < 2:236566/141‑1 (MQ=255) aTGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGGGGTGAAACTACATCATTGTTCTTTAGGTCTTTCCAATGTATAGCCTAAGCAACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTAtc > 2:157094/1‑141 (MQ=255) gTAACAAAACGGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTAtctt > 2:33718/1‑141 (MQ=255) aaCAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTAtcttct < 2:268872/141‑1 (MQ=255) aCTGATAATTAATATACTGCTTATCAATAACATTACGCCCTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTAGTGGCTTCTTAGAATTATCTTCTAATTTa > 2:117857/1‑141 (MQ=255) tGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAACCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTACTTTTTAATTTAtc > 2:64786/1‑141 (MQ=255) cTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGa < 2:113284/141‑1 (MQ=255) taaaTAACATTAATGCGTGGTGAAACTTAATCATTGTTCTTTAGGTATTTCCAATTTAAAGCCTAAGCCACGCACAGTTTTAATAAGTTCTACCTCCTCAGGTCAATCTACTAATTTATCTCTTAAATGACTGATATGTaa > 2:129556/3‑140 (MQ=255) tCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTACTTTATCTCTTAAATGACTGATATGTAc > 2:269051/1‑141 (MQ=255) aaTAACATTAAGCCGTGGTGAAAATTCATGATTGGTCTTTATGGCTTTCCAATTTAGAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTACAt > 2:167090/1‑141 (MQ=255) tAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTACATCAACAAtt > 1:246849/1‑141 (MQ=255) tAAGCCGTGGTGAAACCTCCTCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTACATCAACAAtt < 1:235653/141‑1 (MQ=255) | CTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCGGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTACATCAACAATT > CP000730/1797661‑1797907 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |