Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,883,654 C→T 100% G522D (GGT→GAT)  fmtB1 ← cell surface anchored protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,883,6540CT100.0% 84.6 / ‑6.5 29G522D (GGT→GAT) fmtB1cell surface anchored protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (19/9);  total (19/10)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATCTTGTGTATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTACCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAATTGTTCACATATTCAACACCTTCAG  >  CP000730/1883517‑1883789
                                                                                                                                         |                                                                                                                                       
aaTCTTGTGTATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGGAGTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTAtctc                                                                                                                                      <  1:269841/141‑1 (MQ=255)
 aTCTTGTGTATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGTACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTAtctcc                                                                                                                                     <  2:205692/141‑1 (MQ=255)
      gtgtATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATCTCTTAAAACGAGTATTTTATCAGGCATAAGTCGTGCCGGAGCGTTTGCTGTATCTCATCCat                                                                                                                                >  1:65949/1‑140 (MQ=255)
      gtgtATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCCCCAg                                                                                                                                >  2:135069/1‑141 (MQ=255)
          ttatGTTTTATATGTTGATTTCTCGTTAAATTGTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACt                                                                                                                            <  2:40986/140‑1 (MQ=255)
                    taCGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGtt                                                                                                                  >  1:263096/1‑141 (MQ=255)
                     aCGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTa                                                                                                                 >  2:121540/1‑141 (MQ=255)
                             gTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGa                                                                                                         >  2:18636/1‑141 (MQ=255)
                               ctcGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATTTATAATCCATTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGAtt                                                                                                       <  1:136032/141‑1 (MQ=255)
                               ctcGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGAtt                                                                                                       >  1:189405/1‑141 (MQ=255)
                                ttgTTAAATGTTTCTGTTCTTGGTGTCTGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCATTACTTTTAATTGTTATCACTCGAttt                                                                                                      <  2:65390/139‑1 (MQ=255)
                                tcGTTAAATTCTACTGTCTTTGTTGTCGGCAAATGATTTACGCGTAATTTAGATATTAATTCTATTATTTTATCAGGCATAAGTCGTGCCGGAGATTTTGTTGTATCTCCTCCAGTACTTTTAATTGTTATCACTCGAttt                                                                                                      <  2:250658/141‑1 (MQ=255)
                                  gTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTtgc                                                                                                    <  1:278073/141‑1 (MQ=255)
                                     aaaTGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTtgctgc                                                                                                 <  1:182197/141‑1 (MQ=255)
                                         gTTACTGTTCTTGGTGTCGGCACATTATTTAAACGTAATTTCTATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCTCACTATTTTCTGaatca                                                                                             >  2:228449/1‑141 (MQ=255)
                                              tGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGGTTTGCTGCATCctattt                                                                                        >  1:160874/1‑139 (MQ=255)
                                                       tgtCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCat                                                                               >  2:165421/1‑141 (MQ=255)
                                                             cacaTTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTACTGCCTCCATTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCAtt                                                                         <  2:245279/141‑1 (MQ=255)
                                                                  tattTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACAt                                                                    >  1:6350/1‑141 (MQ=255)
                                                                         acGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGcc                                                             >  1:189450/1‑141 (MQ=255)
                                                                         acGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGcc                                                             >  1:80598/1‑141 (MQ=255)
                                                                                  ttatCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGtt                                                    <  2:286400/140‑1 (MQ=255)
                                                                                  atatCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGtt                                                    >  2:158897/1‑141 (MQ=255)
                                                                                     tcgtAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACt                                                 <  2:13533/138‑1 (MQ=255)
                                                                                               aGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCt                                       >  2:41987/1‑141 (MQ=255)
                                                                                                gtcttttATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCtt                                      <  1:121540/138‑1 (MQ=255)
                                                                                                gTATTTTATCAGGCATACCCCGTTCCTGTGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTCACATCAACGCCTGAATTGTTACTTGGAAAATCtt                                      >  2:203528/1‑141 (MQ=255)
                                                                                                   ttttATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAg                                   >  2:260958/1‑141 (MQ=255)
                                                                                                            gCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAAt                          >  2:112568/1‑141 (MQ=255)
                                                                                                             cATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAAtt                         >  2:23066/1‑141 (MQ=255)
                                                                                                                                    cTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGAAGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAATTGTTCACATATTCAACACCTTCAg  >  1:270035/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                       
AATCTTGTGTATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTACCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAATTGTTCACATATTCAACACCTTCAG  >  CP000730/1883517‑1883789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑